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First Detection of Eustrongylides excisus in Mosquito Fish (Gambusia holbrooki) from Lake Eğirdir

Year 2025, Volume: 21 Issue: 3, 203 - 213, 01.09.2025

Hakan Eren , Vedat Yegen , Muhammed Mustafa Sezginer , Cafer Bulut , Mehmet Oğuz Öztürk

https://doi.org/10.22392/actaquatr.1577775

Abstract

In this study, the presence of Eustrongylides excisus, one of the zoonotic nematodes, was determined for the first time in Gambusia holbrooki from Lake Eğirdir. 70 Gambusia holbrooki samples were examined macroscopically for Eustrongylides excisus infection. The four parasite samples were recorded in 4 fish, one in each fish. The parasitic species identification was performed using the classical method on E. excisus samples isolated from the host fish, then PCR was performed on 18S rDNA. As a result of molecular analyses, 18S rDNA loci fragment of E. excisus was determined to be 941 bases long and registered to the NCBI data system with accession number PP333225. The taxonomic position of the present isolates and other Eustrongylides isolates registered in NCBI was evaluated through a phylogenetic analysis. It was determined that the isolates in this study had similar sequences to the Eustrongylides samples previously reported from Türkiye. In addition, the isolates of this study were found to have close similarity to Eustrongylides isolates from countries geographically close to Türkiye, such as Iran and Italy, while the similarity rate with Eustrongylides isolates from countries such as China, Brazil and India was found to be low. As a result of this study, it was determined for the first time that Gambusia holbrooki plays a role as an intermediate host in the life cycle of E. excisus. In addition, the sequences belonging to the 18S rDNA locus of E. excisus samples from host fish were identified and contributed to the determination of the genetic characteristics of the parasite species.

Keywords

DNA Eustronglydes exicus Gambusia holbrooki Lake Eğirdir

Project Number

TAGEM/HAYSÜD/B/23/A6/P4/5991

References

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Eğirdir Gölündeki Sivrisinek Balığı’nda (Gambusia holbrooki) Eustrongylides excisus‘un İlk Tespiti

Year 2025, Volume: 21 Issue: 3, 203 - 213, 01.09.2025

Hakan Eren , Vedat Yegen , Muhammed Mustafa Sezginer , Cafer Bulut , Mehmet Oğuz Öztürk

https://doi.org/10.22392/actaquatr.1577775

Abstract

Bu çalışmada Eğirdir Gölü Gambusia holbrooki'sinde zoonotik nematodlardan biri olan Eustrongylides excisus'un varlığı ilk kez tespit edilmiştir. 70 Gambusia holbrooki örneği Eustrongylides excisus enfeksiyonu açısından makroskopik olarak incelendi. Her balıkta bir tane olmak üzere 4 balıkta dört parazit örneği kaydedilmiştir. Parazit tür teşhisi, konak balıktan izole edilen E. excisus örnekleri üzerinde klasik yöntem kullanılarak yapılmış, daha sonra 18S rDNA üzerinde PCR gerçekleştirilmiştir. Moleküler analizler sonucunda, E. excisus'un 18S rDNA lokus fragmanının 941 baz uzunluğunda olduğu belirlenmiş ve NCBI veri sistemine PP333225 erişim numarası ile kaydedilmiştir. Mevcut izolatların ve NCBI'da kayıtlı diğer Eustrongylides izolatlarının taksonomik konumu filogenetik bir analiz ile değerlendirilmiştir. Bu çalışmadaki izolatların daha önce Türkiye'den bildirilen Eustrongylides örnekleriyle benzer dizilere sahip olduğu belirlenmiştir. Ayrıca bu çalışmadaki izolatların İran ve İtalya gibi Türkiye'ye coğrafi olarak yakın ülkelerden elde edilen Eustrongylides izolatları ile yakın benzerlik gösterdiği, Çin, Brezilya ve Hindistan gibi ülkelerden elde edilen Eustrongylides izolatları ile benzerlik oranının ise düşük olduğu tespit edilmiştir. Bu çalışma sonucunda Gambusia holbrooki'nin E. excisus'un yaşam döngüsünde ara konak olarak rol oynadığı ilk kez tespit edilmiştir. Ayrıca, konak balıklardaki E. excisus örneklerinin 18S rDNA lokusuna ait sekanslar tanımlanmıştır.

Keywords

DNA Eustronglydes exicus Gambusia holbrooki Eğirdir Gölü

Ethical Statement

Balıklar ağdan ölü olarak temin edildiği için etik kurul izni gerekmemektedir.

Project Number

TAGEM/HAYSÜD/B/23/A6/P4/5991

References

  • Akcimen, U., Emre, N., Bulut, C., et al. (2014). An investigation on Eustrongylides excisus (Jagerskiöld, 1909) parasite in fish of Lake Eğirdir. V. Eastern Anatolia Region Fisheries Symposium, May 31- June 02, Elazığ.
  • Akcimen, U., Apaydın-Yağcı, M., Yeğen, V., et al. (2018). First report of Eustrongylides excisus larvae and Lernaea cyprinacea on Eğirdir minnow (Pseudophoxinus egridiri). In Proceedings of the 13th International Symposium on Fisheries and Aquatic Sciences, Ankara, Turkiye.
  • Alp, A., Akyurek, M., Balık, S., et al. (1994). Akşehir Gölü’nün Bazı Biyoekolojik Özelliklerinin Tespiti Projesi Sonuç Raporu. Eğirdir Su Ürünleri Araştırma Enstitüsü Müdürlüğü, Eğirdir, Isparta.
  • Altschul, S. F., Gish, W., Miller, W., et al. (1990). Basic local alignment search tool. J Mol Biol, 215, 403–410.
  • Apaydın-Yagcı, M., Alp, A., Yagcı, A., et al. (2014). Diet and prey selection of pikeperch (Sander lucioperca Linnaeus,1758) population in Lake Egirdir (Turkey). Arch Biol Sci, 66, 1515–1527. https://doi.org/10.2298/ABS1404515Y
  • Apaydın-Yağcı, M., Alp, A., Yağcı, A., et al. (2018). Feeding ecology and prey selection of sand smelt, Atherina boyeri Risso, 1810 in Eğirdir Lake (Southern Anatolia, Turkey). J Appl Ichthyol, 34, 815–824. https://doi.org/10.1111/jai.13676
  • Aydogdu, N., Emre, N., Aco-Alburqueque, R., et al. (2024). Observations on Helminth Parasites in Endemic and Native Fish from Susurluk Basin, Türkiye Using a Genetic-Molecular Approach: With New Host and Geographical Records. Journal of the Hellenic Veterinary Medical Society, 75(2), 7281-7290. https://doi.org/10.12681/jhvms.32892
  • Aydoğdu, A., Emre, Y., Emre, N., et al. (2011). The occurrence of helminth parasites (Nemathelminthes) in some freshwater fish from streams discharging into Antalya Bay in Antalya, Turkey: Two new host records from Antalya. Turkish Journal of Zoology, 35(6), 859-864. https://doi.org/10.3906/zoo-0912-16
  • Balık, İ., Çubuk, H., Karasahin, B., et al. (2006). Food and Feeding Habits of the Pikeperch, Sander lucioperca (Linnaeus, 1758), Population from Lake Eğirdir (Turkey). Turkish Journal of Zoology, 30(1), 19-26.
  • Bjelić-Čabrilo, O., Novakov, N., Ćirković, M., et al. (2013). Research Note Title: The first determination of Eustrongylides excisus Jägerskiöld, 1909-larvae (Nematoda: Dioctophymatidae) in the pike-perch Sander lucioperca in Vojvodina (Serbia). Helminthologia, 50(4), 291-294. https://doi.org/10.2478/s11687-013-0143-1
  • Coyner, D. F., Spalding, M. G., Forrester, D. J. (2002). Epizootiology of Eustrongylides ignotus in Florida: Distribution, density, and natural infections in intermediate hosts. Journal of wildlife Diseases, 38(3), 483–499. https://doi.org/10.7589/0090-3558-38.3.4823
  • Derycke, S., Remerie, T., Vierstraete, A., Backeljau, T., Vanfleteren, J., Vincx, M., & Moens, T. (2005). Mitochondrial DNA variation and cryptic speciation within the free-living marine nematode Pellioditis marina. Marine Ecology Progress Series, 300, 91-103.
  • Doğan, U., Şimşek, M., Akçay, İ., Koçyiğit, A. (2025). Eğirdir Gölü’nün düdenleri: Göl çukurluğu bir polye mi?. Jeomorfolojik Araştırmalar Dergisi(14), 31-47. https://doi.org/10.46453/jader.1605722
  • Ekmekci, F. G., Serife, G., Kırankaya, S. G., et al. (2013). Türkiye İçsularındaki İstilacı Balıkların Güncel Durumu ve İstilanın Etkilerinin Değerlendirilmesi. Istanbul University Journal of Fisheries & Aquatic Sciences, 28, 105-140.
  • Franceschini, R., Guardone, L., Armani, A., Ranucci, D., Roila, R., Valiani, A., ... & Branciari, R. (2022). Five-years management of an emerging parasite risk (Eustrongylides sp., Nematoda) in a fishery supply chain located on Trasimeno Lake (Italy). Food Control, 136.
  • Geldiay, R., Balık, S. (2009). Türkiye Tatlısu Balıkları. Ege Üniv. Su Ürünleri Fakültesi Yayınları No:46, Bornova, İzmir.
  • Guardone, L., Ricci, E., Susini, F., et al. (2021). First detection of E. excisus (Nematoda: Dioctophymatidae) in big-scale sand smelt (Atherina boyeri) from the lake Massaciuccoli (Northwest Tuscany, Italy). Parasitology Research, 109, 769–780. https://doi.org/10.1016/j.foodcont.2020.107517
  • Guven, A., Ozturk, T. (2018). Metazoan parasite faunas of three Gobiid species (Actinopterygii: Gobiidae) inhabiting the lower Kızılırmak Delta in Samsun: A comparative study. Türkiye Parazitol Derg, 42(1), 33-38. https://doi.org/10.5152/tpd.2018.5635
  • Hall, T. (2013). BioEdit: Biological sequence alignment editor for Win95/98/NT/2K/XP, 95–98.
  • Innal, D., Yıldırım, M., Stavrescu-Bedivan, M. M., et al. (2019). Occurrence and infection dynamics of Salsuginus sp. (Monogenea, Ancyrocephalidae) and Eustrongylides excisus (Nematoda, Dioctophymatidae) in four endemic Aphanius (Cyprinodontidae) species: new host records for Turkish fauna. Acta Biologica Turcica, 32(2), 103- 109.
  • İnnal, D., (2022). Use of fishes as biological control agents for prevention of malaria in Turkey: A journey to history, Acta Biologica Turcica, 35(1), 36–45.
  • Juhásová, Ľ., Radačovská, A., Bazsalovicsova, E., et al. (2019). A study of the endohelminths of the European perch Perca fluviatilis L. from the central region of the Danube river basin in Slovakia. ZooKeys, 899, 47-58. https://doi.org/10.3897/zookeys.899.39638
  • Kosks. (2024). Ministry of Agriculture and Forestry. Mid-Winter Bird Count Report 2024. KOSKS_Raporu_2024.pdf Accessed, 13, 4.
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There are 52 citations in total.

Details

Primary Language English
Subjects Aquaculture and Fisheries (Other)
Journal Section Research Articles
Authors

Hakan Eren 0000-0001-8475-4237

Vedat Yegen 0000-0001-5349-1497

Muhammed Mustafa Sezginer 0000-0001-9812-7605

Cafer Bulut 0000-0002-5525-170X

Mehmet Oğuz Öztürk 0000-0001-7263-3585

Project Number TAGEM/HAYSÜD/B/23/A6/P4/5991
Early Pub Date June 3, 2025
Publication Date September 1, 2025
Submission Date November 1, 2024
Acceptance Date March 21, 2025
Published in Issue Year 2025 Volume: 21 Issue: 3

Cite

APA Eren, H., Yegen, V., Sezginer, M. M., Bulut, C., et al. (2025). First Detection of Eustrongylides excisus in Mosquito Fish (Gambusia holbrooki) from Lake Eğirdir. Acta Aquatica Turcica, 21(3), 203-213. https://doi.org/10.22392/actaquatr.1577775
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