Nigella sativa L.'da Zigotik Ovül Kültürü Üzerine Farklı Konsantrasyonlarda IAA ve BAP Uygulamasının Etkisi
Abstract
Bu çalışma BAP (6-Benzylaminopurine) ve IAA (Indole-3-acetic acid) hormonlarının Çörek otu bitkisinde in vitro kültürde embriyo gelişimi üzerine etkisini araştırmak ve melez bitkiler elde etmek amacıyla gerçekleştirilmiştir. Tohumların çok küçük olması ve embriyo izolasyonunun zor olması nedeniyle çalışmada ovül kültürü tercih edilmiştir. Ovül kültürleri, Türkiye'de Ankara ve Denizli'deki üreticilerden elde edilen popülasyonlar ve Çameli çeşidi kullanılarak yapılmıştır. Melezlemeler, zigotik embriyolar elde etmek için yarı diallel melezleme yöntemine göre yapılmıştır. Ovül kültürü için BAP veya IAA (0.0, 0.5, 1.0, 2.0 mg/l) ve BAP+IAA (1.0+1.0, 0.1+2.0, 2.0+0.5 mg/l) eklenmiş MS (Duchefa Biochemie, Katalog No: M0222) besiyerleri kullanılmıştır. Araştırma sonucunda en yüksek başarı doğrudan rejenerasyonda (%1,7) hormonsuz ortamda elde edilmiştir. Kallus rejenerasyonunda en yüksek başarı (%2,3) 2,0 mg/l BAP + 0,5 mg/l IAA eklenmiş ortamda elde edilmiştir. Kallustan en yüksek bitki rejenerasyonu %7,7 oranında 1,0 mg/l IAA eklenmiş ortamda elde edilmiştir.
Keywords
Project Number
ZRF-19028
References
- Al-Ani NK (2008) Thymol production from callus culture of Nigella sativa L.. Plant Tissue Culture & Biotechnology, 18:181-185.
- Burdock G (2022) Assessment of black cumin (Nigella sativa L.) as a food ingredient and putative therapeutic agent. Regulatory Toxicology and Pharmacology.; 128:105088.
- Chaudhry H, Fatima N, Ahmad IZ (2014) Establishment of callus and cell suspension cultures of Nigella sativa L. For thymol production. International Journal of Pharmacy and Pharmaceutical Sciences, 6: 0975-1491.
- Damanik RI, Sinuraya M, Setiado H (2022). The role of auxin and giberalin in the growth of black cumin (Nigella sativa L.) by in vitro. IOP Conference Series: Earth and Environmental Science, 18 November 2021, Medan, Indonesia. Volume 977: 1-5.
- Dekami A, Sanjarian F, Chaichi M, Hosseini B, Rahnama H (2023). Effect of Plant Growth Regulators and Light on Callus Induction, Antioxidant Enzyme Response and Total Phenol in Nigella arvensis L. Journal of Medicinal plants and By-product, 12(1): 47-56.
- Drew RA (1997) The Application of Biotechnologyto the Conservation and Improvement of Tropical and Subtropical Fruit Species. Seed and Plant Genetic Resources Service Food and Agriculture Organization of the United Nations Rome.
- El-Mahrouk ME, Maamoun MK, EL-Banna AN, Omran SA, Dewir YH, El-Hendawy S (2018). In Vitro Gynogenesis and Flow Cytometry Analysis of the Regenerated Haploids of Black Cumin (Nigella sativa). HortScience horts, 53(5): 681-686. Retrieved Aug 15, 2023.
- ElNour EM, Mahmood ZA, Futooh Y, Sanaa O (2015). In Vitro Callus Induction and Antimicrobial Activities of Callus and Seeds Extracts of Nigella sativa L. Journal of Biology, 3(3): 21-28.
- Hatipoğlu R (2012) Bitki Biyoteknolojisi. Textbook, Çukurova University, Agricultural Faculty, Field Crop Department, Number: 176, Adana, TURKEY
- Klimek-Chodacka M, Kadluczka D, Lukasiewicz A, Malec-Pala A, Baranski R, Grzebelus E (2020). Effective callus induction and plant regeneration in callus and protoplast cultures of Nigella damascena L.. Plant Cell Tiss Organ Cult 143, 693–707.
- Kurt O, Şavşatlı Y (2005) Bitkisel Biyoteknolojiye Genel Bir Bakış. Journal of Ondokuz Mayıs University Agricultural Faculty 20 (3):126-133.
- Narayanaswami S, Norstog K (1964) Plant embryo culture. Botanical Review 30 (4): 587-628.
- Rezaei F, Isik S, Kartal, M, Erdem SA (2018). Effect of priming on thymoquinone content and in vitro plant regeneration with tissue culture of black cumin (Nigella sativa L.) seeds. Journal of Chemical Metrology, 12(2), 98.
- Seçmen Ö, Gemici Y, Görk G, Bekat L, Leblebici E (2000) Tohumlu Bitkiler Sistematiği. Textbook, Ege University, Science Faculty Number: 116. İzmir, TURKEY.
- Uysal H (2021) In vitro propagation of black cumin (Nigella sativa L.) plants, Genetika-Belgrade, 53(1): 295-303.
- Uysal H, Sevindik E (2021). Development of breeding lines by zigotic ovule culture in Nigella sativa L. Genetika. 53(2), 583-591.
Effect of IAA and BAP Application in Varying Concentration on Zygotic Ovule Culture in Nigella sativa L.
Abstract
This study was carried out to test the effect of BAP (6-Benzylaminopurine) and IAA (Indole-3-acetic acid) hormones, both on embryo development in in vitro culture, and on obtaining hybrid plants of black cumin. Ovule culture was preferred in the study because the seeds were too small and embryo isolation was difficult. Ovule cultures were done by using populations obtained from producers in Ankara and Denizli in Türkiye and the Çameli variety. Hybridizations were done according to the semi-diallel hybridization method to gain zygotic embryos. MS (Duchefa Biochemie, Catalog No: M0222) mediums, with BAP or IAA (0.0, 0.5, 1.0, 2.0 mg/l) and BAP+IAA (1.0+1.0, 0.1+2.0, 2.0+0.5 mg/l) added, were used for ovule culture. As a result of the research, the highest success was gained for direct regeneration (1.7%) in the medium without hormone. The highest success gained for callus regeneration (2.3%) was in the medium having 2.0 mg/l BAP + 0.5 mg/l IAA added. The highest plant regeneration from callus was gained in the medium with an added 1.0 mg/l IAA as 7.7%.
Ethical Statement
Gerektirmemekte
Supporting Institution
Aydın Adnan Menderes University
Project Number
ZRF-19028
Thanks
This research was financially supported by the Scientific Research Project of Aydin Adnan Menderes University (Project Number: ZRF-19028). The authors would like to thanks for their support. We also wish to thanks C. E. Fiero for editorial assistance on this article.
References
- Al-Ani NK (2008) Thymol production from callus culture of Nigella sativa L.. Plant Tissue Culture & Biotechnology, 18:181-185.
- Burdock G (2022) Assessment of black cumin (Nigella sativa L.) as a food ingredient and putative therapeutic agent. Regulatory Toxicology and Pharmacology.; 128:105088.
- Chaudhry H, Fatima N, Ahmad IZ (2014) Establishment of callus and cell suspension cultures of Nigella sativa L. For thymol production. International Journal of Pharmacy and Pharmaceutical Sciences, 6: 0975-1491.
- Damanik RI, Sinuraya M, Setiado H (2022). The role of auxin and giberalin in the growth of black cumin (Nigella sativa L.) by in vitro. IOP Conference Series: Earth and Environmental Science, 18 November 2021, Medan, Indonesia. Volume 977: 1-5.
- Dekami A, Sanjarian F, Chaichi M, Hosseini B, Rahnama H (2023). Effect of Plant Growth Regulators and Light on Callus Induction, Antioxidant Enzyme Response and Total Phenol in Nigella arvensis L. Journal of Medicinal plants and By-product, 12(1): 47-56.
- Drew RA (1997) The Application of Biotechnologyto the Conservation and Improvement of Tropical and Subtropical Fruit Species. Seed and Plant Genetic Resources Service Food and Agriculture Organization of the United Nations Rome.
- El-Mahrouk ME, Maamoun MK, EL-Banna AN, Omran SA, Dewir YH, El-Hendawy S (2018). In Vitro Gynogenesis and Flow Cytometry Analysis of the Regenerated Haploids of Black Cumin (Nigella sativa). HortScience horts, 53(5): 681-686. Retrieved Aug 15, 2023.
- ElNour EM, Mahmood ZA, Futooh Y, Sanaa O (2015). In Vitro Callus Induction and Antimicrobial Activities of Callus and Seeds Extracts of Nigella sativa L. Journal of Biology, 3(3): 21-28.
- Hatipoğlu R (2012) Bitki Biyoteknolojisi. Textbook, Çukurova University, Agricultural Faculty, Field Crop Department, Number: 176, Adana, TURKEY
- Klimek-Chodacka M, Kadluczka D, Lukasiewicz A, Malec-Pala A, Baranski R, Grzebelus E (2020). Effective callus induction and plant regeneration in callus and protoplast cultures of Nigella damascena L.. Plant Cell Tiss Organ Cult 143, 693–707.
- Kurt O, Şavşatlı Y (2005) Bitkisel Biyoteknolojiye Genel Bir Bakış. Journal of Ondokuz Mayıs University Agricultural Faculty 20 (3):126-133.
- Narayanaswami S, Norstog K (1964) Plant embryo culture. Botanical Review 30 (4): 587-628.
- Rezaei F, Isik S, Kartal, M, Erdem SA (2018). Effect of priming on thymoquinone content and in vitro plant regeneration with tissue culture of black cumin (Nigella sativa L.) seeds. Journal of Chemical Metrology, 12(2), 98.
- Seçmen Ö, Gemici Y, Görk G, Bekat L, Leblebici E (2000) Tohumlu Bitkiler Sistematiği. Textbook, Ege University, Science Faculty Number: 116. İzmir, TURKEY.
- Uysal H (2021) In vitro propagation of black cumin (Nigella sativa L.) plants, Genetika-Belgrade, 53(1): 295-303.
- Uysal H, Sevindik E (2021). Development of breeding lines by zigotic ovule culture in Nigella sativa L. Genetika. 53(2), 583-591.
Details
| Primary Language | English |
|---|---|
| Subjects | Agricultural Engineering (Other) |
| Journal Section | Research |
| Authors | |
| Project Number | ZRF-19028 |
| Publication Date | June 30, 2025 |
| Submission Date | April 28, 2025 |
| Acceptance Date | June 13, 2025 |
| Published in Issue | Year 2025 Volume: 22 Issue: 1 |
