Research Article
Year 2015,
Volume: 16 Issue: 2, 176 - 186, 13.01.2016
Abstract
Bu çalışmada, halk arasında sebze olarak tüketilen E. spectabilis Bieb.’in in vitro antioksidan ve antimikrobiyal aktivitesi incelenmiş ve anaerobik kültür ortamında Kluyveromyces lactis üzerinde ki antioksidan aktivitesi araştırılmıştır. Bu amaçla; 2,2-difenil-1-pikrilhidrazil (DPPH) giderme aktivitesi, flavonoid, resveratrol, şeker içeriği, lipid peroksidasyon düzeyi, yağ asidi düzeyi, lipofilik vitamin değerleri, protein ve glutatyon miktarları ölçüldü. FeCl2+H2O2 uygulanan FeCl grubunda LPO seviyesinin kontrol grubuna (K) kıyasla yüksek miktarda arttığı görüldü (p<0.05). Tedavi gruplarında da LPO düzeyinin belli oranlarda azaldığı belirlendi. Eremurus spectabilis’in LPO düşürücü etkisinin içerdiği flavonoidler gibi fitokimyasal kaynaklar olduğu ileri sürülmektedir. Antibakteriyel aktivite sonuçlarına göre E. spectabilis’in E. coli, S. aureus, Epidermophyton spp. ve C. albicans’a karşı farklı oranlarda antimikrobiyal aktiviteye sahip olduğu görülmektedir.
References
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- Collins C, Lyne P (1987) Microbiological Methods Butter Morths&Co (Publishers) Ltd. London. 450pp
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- Pekkarinen S S, Heinonen I M, Hopia A I (1999) Flavonoids quercetin, myricetin, kaemferol and (+)‐catechin as antioxidants in methyl linoleate, Journal of the Science of Food and Agriculture 79: 499-506
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- Shimoi K, Masuda S, Furugori M, Esaki S, Kinae N (1994) Radioprotective effect of antioxidative flavonoids in γ-ray irradiated mice, Carcinogenesis 15: 2669-2672
- Sokół-Łętowska A, Oszmiański J, Wojdyło A (2007) Antioxidant activity of the phenolic compounds of hawthorn, pine and skullcap, Food Chemistry 103: 853-859
- Taskin T, Bitis L, Birteksöz S (2012) Antioxidant and antimicrobial activities of different extracts from Eremurus spectabilis leaves, Spatula DD-Peer Reviewed Journal on Complementary Medicine and Drug Discovery 2: 213-217
- Wang K, Posner A, Hajimohammadreza I (1996) Total protein extraction from cultured cells for use in electrophoresis and western blotting, BioTechniques 20: 662-668
- Yilmaz O, Ozsahin A D, Bircan B, Erden Y, Karaboga Z (2010) Radical Scavenging Activity of the Pistacia terebinthus in Fenton Reagent Environment and Its Protective Effects on the Unsaturated Fatty Acids, Asian Journal of Chemistry 22: 7949
- Zu Y, Li C, Fu Y, Zhao C (2006) Simultaneous determination of catechin, rutin, quercetin kaempferol and isorhamnetin in the extract of sea buckthorn (Hippophae rhamnoides L.) leaves by RP-HPLC with DAD, Journal of Pharmaceutical and Biomedical Analysis 41: 714-719.
Year 2015,
Volume: 16 Issue: 2, 176 - 186, 13.01.2016
Abstract
In this study, in vitro antioxidant and antimicrobial activities of the E. spectabilis Bieb., which is consumed as a vegetable among people, was investigated and Kluyveromyces lactis’s antioxidant activies in anaerobic cultural environment is researched. This purpose; 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, flavonoid, resveratrol, sugar contents, lipid peroxidation (LPO) levels, fatty acid level, lipophilic vitamin values, protein and glutathione amounts were measured. In the FeCl group in which FeCl2+ H2O2 is applied, when compared with LPO level control group (K), it was observed that it increase in high amount (p<0.05). LPO level decreases in certain proportions in treatment groups. It is suggested that the lowering effect of the LPO of E. spectabilis extracts are arise from phytochemical like flavonoids that it includes. As a result of the antibacterial activity studies, it was observed that E. spectabilis have different proportional antimicrobial activities against E. coli, S. aureus, Epidermophyton spp. and C. albicans.
References
- Akkaya H, Yilmaz O (2012) Antioxidant capacity and radical scavenging activity of Silybum marianum and Ceratonia siliqua, Ekoloji 21: 9-16
- Altundag E, Ozturk M (2011) Ethnomedicinal studies on the plant resources of east Anatolia, Turkey, Procedia-Social and Behavioral Sciences 19: 756-777
- Arıtuluk Z C, Ezer N (2012) Halk Arasında Diyabete Karşı Kullanılan Bitkiler (Türkiye)-II, Hacettepe Üniversitesi Eczacılık Fakültesi Dergisi 2: 179-208
- Asgarpanah J, Amin G, Parviz M (2013) In vitro antiglycation activity of Eremurus persicus (Jaub. Et Sp.) Boiss, African Journal of Biotechnology 10: 11287-11289
- Berger A, Jones P, Abumweis S S (2004) Plant sterols: factors affecting their efficacy and safety as functional food ingredients, Lipids Health Dis 3: 1-19
- Bors W, Saran M (1987) Radical scavenging by flavonoid antioxidants, Free Radical Research 2: 289-294
- Brand-Williams W, Cuvelier M, Berset C (1995) Use of a free radical method to evaluate antioxidant activity, LWT-Food Science and Technology 28: 25-30
- Collins C, Lyne P (1987) Microbiological Methods Butter Morths&Co (Publishers) Ltd. London. 450pp
- De Las Heras A, Schoch A, Gibis M, Fischer A (2003) Comparison of methods for determining malondialdehyde in dry sausage by HPLC and the classic TBA test, European food research and technology 217: 180-184
- Di Carlo G, Mascolo N, Izzo A A, Capasso F (1999) Flavonoids: old and new aspects of a class of natural therapeutic drugs, Life sciences 65: 337-353
- Ellman G L (1959) Tissue sulfhydryl groups, Archives of biochemistry and biophysics 82: 70-77
- Erden Y, Bircan B, Yılmaz Ö, Erecevit P (2013) Antioxidative Activity and Phytochemical Composition of Dried Apricot, Turkish Journal of Science & Technology 8: 107-113
- Folmer V, Pedroso N, Matias A C, Lopes S C, Antunes F, Cyrne L, Marinho H S (2008) H2O2 induces rapid biophysical and permeability changes in the plasma membrane of Saccharomyces cerevisiae, Biochimica et Biophysica
- Acta (BBA)-Biomembranes 1778: 1141-1147
- Formica J, Regelson W (1995) Review of the biology of quercetin and related bioflavonoids, Food and chemical toxicology 33: 1061-1080
- Grace C 2004. Chromatography A. A Grace Company Catolog 600. Alltech Associates Inc, U.S
- Hopia A, Heinonen M (1999) Antioxidant activity of flavonol aglycones and their glycosides in methyl linoleate, Journal of the American Oil Chemists' Society 76: 139-144
- Kırbag S, Bagcı E (2000) An Investigation on The Antimicrobial Activity of The Picea abies (L.) KARST and Picea orientalis (L.) LINK Essential Oils, Journal of Qafqaz University 3: 183-190
- Laemmli U K (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature 227: 680-685
- Lopez-Cervantes J, Sanchez-Machado D, Rios-Vazquez N (2006) High-performance liquid chromatography method for the simultaneous quantification of retinol, α-tocopherol, and cholesterol in shrimp waste hydrolysate, Journal of Chromatography A 1105: 135-139
- Lowry O H, Rosebrough N J, Farr A L, Randall R J (1951) Protein measurement with the Folin phenol reagent, J biol Chem 193: 265-275
- Mcdonough V M, Roth T M (2005) Growth temperature affects accumulation of exogenous fatty acids and fatty acid composition in Schizosaccharomyces pombe, Antonie van Leeuwenhoek 86: 349-354
- Pekkarinen S S, Heinonen I M, Hopia A I (1999) Flavonoids quercetin, myricetin, kaemferol and (+)‐catechin as antioxidants in methyl linoleate, Journal of the Science of Food and Agriculture 79: 499-506
- Pinheiro R, Belo I, Mota M (2002) Oxidative stress response of Kluyveromyces marxianus to hydrogen peroxide, paraquat and pressure, Applied microbiology and biotechnology 58: 842-847
- Prior R L (2003) Fruits and vegetables in the prevention of cellular oxidative damage, The American journal of clinical nutrition 78: 570S-578S
- Sánchez-Moreno C, Larrauri J A, Saura-Calixto F (1999) Free radical scavenging capacity and inhibition of lipid oxidation of wines, grape juices and related polyphenolic constituents, Food Research International 32: 407-412
- Shimoi K, Masuda S, Furugori M, Esaki S, Kinae N (1994) Radioprotective effect of antioxidative flavonoids in γ-ray irradiated mice, Carcinogenesis 15: 2669-2672
- Sokół-Łętowska A, Oszmiański J, Wojdyło A (2007) Antioxidant activity of the phenolic compounds of hawthorn, pine and skullcap, Food Chemistry 103: 853-859
- Taskin T, Bitis L, Birteksöz S (2012) Antioxidant and antimicrobial activities of different extracts from Eremurus spectabilis leaves, Spatula DD-Peer Reviewed Journal on Complementary Medicine and Drug Discovery 2: 213-217
- Wang K, Posner A, Hajimohammadreza I (1996) Total protein extraction from cultured cells for use in electrophoresis and western blotting, BioTechniques 20: 662-668
- Yilmaz O, Ozsahin A D, Bircan B, Erden Y, Karaboga Z (2010) Radical Scavenging Activity of the Pistacia terebinthus in Fenton Reagent Environment and Its Protective Effects on the Unsaturated Fatty Acids, Asian Journal of Chemistry 22: 7949
- Zu Y, Li C, Fu Y, Zhao C (2006) Simultaneous determination of catechin, rutin, quercetin kaempferol and isorhamnetin in the extract of sea buckthorn (Hippophae rhamnoides L.) leaves by RP-HPLC with DAD, Journal of Pharmaceutical and Biomedical Analysis 41: 714-719.
There are 32 citations in total.
Details
| Journal Section | Research Article |
|---|---|
| Authors | |
| Publication Date | January 13, 2016 |
| Published in Issue | Year 2015 Volume: 16 Issue: 2 |
Cite
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