İki farklı tip tahribatsız portatif klorofil metre kullanarak Artvin’de yayılış gösteren sapsız meşede (Quercus, Fagaceae) klorofil tahmini
Abstract
Bu çalışmada, SPAD-502Plus ve CCM-300 adlı optik klorofil
içerik ölçer cihazlarından elde edilen klorofil içerik değerleri ile %80 aseton
ile klorofil ekstraksiyon metodundan elde edilen klorofil içerik değerleri
arasındaki ilişkinin saptanması amaçlanmıştır. Bu çalışma, sapsız meşenin, Quercus petraea (Mattuschka) Liebl.
subsp. iberica (Steven ex Bieb.)
Krassiln. alt türü üzerinde gerçekleştirilmiştir. Rakımı 889 m olan önceden
belirlenmiş bir alandan benzer özelliklere sahip 11 ağaç seçilmiş, sağlıklı
toplam 44 yaprak üzerinde ölçümler gerçekleştirilmiştir. Yapraklara ait SPAD,
CFR (klorofil floresans) değerleri ve %80 aseton ile klorofil ekstraksiyonu
devamında yapılan spektrofotometrik okumalar sonucunda elde edilen bulgular
ayrı ayrı değerlendirilmiştir. Ölçümler sonucunda, her üç ölçüm tipi (SPAD, CFR
ve %80 aseton metodu) sonuçlarının kuvvetli ilişki sergilediği tespit
edilmiştir. Elde edilen SPAD ve CFR değerlerinin %80 aseton ile ekstraksiyon
metodundan elde edilen verilere dönüştürülmesi amacıyla regresyon analizleri
ile çeşitli eşitlikler oluşturulmuştur. Bu eşitlikler kullanılarak, SPAD ve CFR
değerlerinin, kimyasal metodla tespit edilmiş klorofil içerik değerlerine
dönüşümü mümkün olabilmektedir. Bu çalışmada, bu meşe türü için, birim alan
başına yaprak toplam klorofil konsantrasyonu, SPAD (R2=0.9367) ve
CFR (R2 = 0.8702) okumaları arasındaki ilişkinin, taze ağırlık
başına yaprak toplam klorofil konsantrasyonuna göre (SPAD (R2 =
0.8264) ve CFR (R2 = 0.7847)) daha yüksek olduğu tespit edilmiştir.
Bu sonuçlara göre, bu optik cihazları kullanarak, tercihen birim alan başına
(mg cm-2), yaprak toplam klorofil içeriği çok kısa sürede ve
kolaylıkla yapraklara zarar vermeden belirlenebilir.
References
- Arnon DI (1949) Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant Physiology, 24:1-15.
- Bielinis E, Jozwiak W, Robakowski P (2015) Modelling of the relationship between the SPAD values and photosynthetic pigments content in Quercus petraea and Prunus serotina leaves. Dendrobiology, vol. 73, 125-134.
- Curran PJ, Dungan JL, Gholz HL (1990) Exploring the relationship between reflectance red edge and chlorophyll content in slash pine. Tree Physiology, 7: 33–48.
- Filella I, Serrano L, Serra J, Peñuelas J (1995) Evaluating wheat nitrogen status with canopy reflectance indices and discriminant analysis. Crop Science, 35: 1400–1405.
- Fujimura S, Shi P, Iwama K, Zhang X, Gopal J, Jitsuyama Y (2009) Comparison of growth and grain yield of spring wheat in Lhasa, the Tibetan Plateau, with those in Sapporo, Japan. Plant Prod. Sci., 12, 116-123.
- Gamon JA, Surfus JS (1999) Assessing leaf pigment content and activity with a reflectometer. New Phytologist, 143: 105–117.
- Gitelson AA, Buschmann C, Lichtenthaler HK (1999) The Chlorophyll Fluorescence Ratio F735/F700 as an Accurate Measure of Chlorophyll Content in Plants Remote Sens. Enviro., 69: 296-302.
- Larcher W (1995) Physiological Plant Ecology, 3rd edn. Berlin, Germany: Springer.
- Markwell J, Osterman JC, Mitchell JL (1995) Calibration of the Minolta SPAD-502 leaf chlorophyll meter. Photosynthesis Research, 46: 467–472.
- Monje OA, Bugbee B (1992) Inherent limitations of nondestructive chlorophyll meters: a comparison of two types of meters. Hortscience, 27: 69–71.
- Moran JA, Mitchell AK, Goodmanson G, Stockburger KA (2000) Differentiation among effects of nitrogen fertilization treatments on conifer seedlings by foliar reflectance: a comparison of methods. Tree Physiology, 20: 1113–1120.
- Peñuelas J, Filella I (1998) Visible and near-infrared reflectance techniques for diagnosing plant physiological status. Trends in Plant Science, 3: 151–156.
- Percival GC, Keary IP, Noviss K (2008) The potential of a chlorophyll content SPAD meter to quantify nutrient stress in foliar tissue of sycamore (Acer pseudoplatanus), english oak (Quercus robur), and european beech (Fagus sylvatica). Arboriculture & Urban Forestry 34: 89–100. Pinkard EA, Patel V, Mohammed C (2006) Chlorophyll and nitrogen determination for plantation-grown Eucalyptus nitens and E. globules using a non-destructive meter. Forest Ecol. 223:211–217. Richardson AD, Duigan SP, Berlyn GP (2002) An evaluation of noninvasive methods to estimate foliar chlorophyll content. New Phytologist, 153: 185–194.
- Sardoel AS, Rahbarian P, Shahdadneghad M (2014) Evaluation chlorophyll contents assessment on three indoor ornomental plants with plant growth regulators. European Journal of Experimental Biology 4:306-310.
Estimation of chlorophyll in sessile oak (Quercus, Fagaceae) naturally distributed in Artvin province using two different types of non-destructive portable chlorophyll meter
Abstract
In this
study, it was aimed to determine the relationship between the chlorophyll
content values obtained from SPAD-502Plus and CCM-300 optical chlorophyll
content meters and the chlorophyll content values obtained from the chlorophyll
extraction method using 80% acetone. This study was carried out on a subspecies
of the sessile oak (Quercus petraea
(Mattuschka) Liebl. subsp. iberica
(Steven ex Bieb.) Krassiln.). 11 trees with similar characteristics were selected from a predefined
area with an altitude of 889 m and measurements were made on a total of 44
healthy leaves. The leaf SPAD values, CFR (chlorophyll fluorescence) values and the results obtained
from the spectrophotometric readings of the chlorophyll extraction with 80%
acetone were evaluated separately. As a result of the
measurements, it was determined that the results of all three measurement types
(SPAD, CFR and 80% acetone method) showed a strong correlation. Various equations were formed by regression
analyses in order to convert the obtained SPAD and CFR values to the values
obtained from the 80% acetone method. Using these equations, it is possible to convert SPAD and CFR values into
chlorophyll content values determined by chemical method. In this study,
for this type of oak, the relationship between the leaf total chlorophyll
concentration per unit area and SPAD (R2 = 0.9367) and CFR (R2
= 0.8702) readings was found higher than
the leaf total chlorophyll concentration per fresh weight (SPAD (R2
= 0.8264) and CFR (R2 = 0.7847)). According to these results, by using these optical devices,
preferably per unit area (mg cm-2), the leaf total chlorophyll content
can be determined in a very short time and easily without damaging the leaves.
References
- Arnon DI (1949) Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant Physiology, 24:1-15.
- Bielinis E, Jozwiak W, Robakowski P (2015) Modelling of the relationship between the SPAD values and photosynthetic pigments content in Quercus petraea and Prunus serotina leaves. Dendrobiology, vol. 73, 125-134.
- Curran PJ, Dungan JL, Gholz HL (1990) Exploring the relationship between reflectance red edge and chlorophyll content in slash pine. Tree Physiology, 7: 33–48.
- Filella I, Serrano L, Serra J, Peñuelas J (1995) Evaluating wheat nitrogen status with canopy reflectance indices and discriminant analysis. Crop Science, 35: 1400–1405.
- Fujimura S, Shi P, Iwama K, Zhang X, Gopal J, Jitsuyama Y (2009) Comparison of growth and grain yield of spring wheat in Lhasa, the Tibetan Plateau, with those in Sapporo, Japan. Plant Prod. Sci., 12, 116-123.
- Gamon JA, Surfus JS (1999) Assessing leaf pigment content and activity with a reflectometer. New Phytologist, 143: 105–117.
- Gitelson AA, Buschmann C, Lichtenthaler HK (1999) The Chlorophyll Fluorescence Ratio F735/F700 as an Accurate Measure of Chlorophyll Content in Plants Remote Sens. Enviro., 69: 296-302.
- Larcher W (1995) Physiological Plant Ecology, 3rd edn. Berlin, Germany: Springer.
- Markwell J, Osterman JC, Mitchell JL (1995) Calibration of the Minolta SPAD-502 leaf chlorophyll meter. Photosynthesis Research, 46: 467–472.
- Monje OA, Bugbee B (1992) Inherent limitations of nondestructive chlorophyll meters: a comparison of two types of meters. Hortscience, 27: 69–71.
- Moran JA, Mitchell AK, Goodmanson G, Stockburger KA (2000) Differentiation among effects of nitrogen fertilization treatments on conifer seedlings by foliar reflectance: a comparison of methods. Tree Physiology, 20: 1113–1120.
- Peñuelas J, Filella I (1998) Visible and near-infrared reflectance techniques for diagnosing plant physiological status. Trends in Plant Science, 3: 151–156.
- Percival GC, Keary IP, Noviss K (2008) The potential of a chlorophyll content SPAD meter to quantify nutrient stress in foliar tissue of sycamore (Acer pseudoplatanus), english oak (Quercus robur), and european beech (Fagus sylvatica). Arboriculture & Urban Forestry 34: 89–100. Pinkard EA, Patel V, Mohammed C (2006) Chlorophyll and nitrogen determination for plantation-grown Eucalyptus nitens and E. globules using a non-destructive meter. Forest Ecol. 223:211–217. Richardson AD, Duigan SP, Berlyn GP (2002) An evaluation of noninvasive methods to estimate foliar chlorophyll content. New Phytologist, 153: 185–194.
- Sardoel AS, Rahbarian P, Shahdadneghad M (2014) Evaluation chlorophyll contents assessment on three indoor ornomental plants with plant growth regulators. European Journal of Experimental Biology 4:306-310.
Details
| Primary Language | Turkish |
|---|---|
| Journal Section | Research Article |
| Authors | |
| Publication Date | April 15, 2019 |
| Acceptance Date | January 30, 2019 |
| Published in Issue | Year 2019 Volume: 20 Issue: 1 |
Cite
Cited By
İki Farklı Vermikültürün Marul (Lactuca sativa L.) Bitkisinin Gelişimi Üzerindeki Etkisi
Karadeniz Fen Bilimleri Dergisi
https://doi.org/10.31466/kfbd.1311076
Artvin Coruh University Journal of Forestry Faculty is licensed under a Creative Commons Attribution 4.0 International License.