Antioxidant activity and total phenolic contents of Galanthus woronowii (Amaryllidaceae).

Nusret Genç; İlyas Yıldız; Tunay Karan; Özgür Eminağaoğlu; Ramazan Erenler

doi:10.38059/biodiversity.515111

Research Article

Galanthus woronowii (Amaryllidaceae)'nin antioksidan aktivitesi ve toplam fenolik içeriği.

Year 2019, Volume: 2 Issue: 1, 1 - 5, 31.03.2019

Nusret Genç , İlyas Yıldız Tunay Karan Özgür Eminağaoğlu Ramazan Erenler

https://doi.org/10.38059/biodiversity.515111

Cited By: 20

Abstract

Bitkiler
eski çağlardan beri tıbbi amaçlarla kullanılmaktadır. İçerdikleri biyoaktif
sekonder metabolitlerden dolayı, bitkiler ilaç keşfi ve gelişimi için oldukça
fazla ilgi görmektedir. Bu çalışmada, Galanthus
woronowii türüne ait bitki materyalleri sırasıyla hekzan, diklorometan ve
etil asetat ile ekstrakte edilmiştir. Çözücü dönerli buharlaştırıcı ile
uzaklaştırıldıktan sonra ham ekstraktlar elde edilmiştir. Ekstraktların DPPH
serbest radikal giderme, ABTS radikal katyon giderme ve indirgeme gücü
aktiviteleri değerlendirilmiştir. Ayrıca toplan fenolik içerik belirlenmiştir.
Etil asetat ekstraktının en çok fenolik içerdiği ve en yüksek antioksidan
aktivite gösterdiği belirlenmiştir. Bu nedenlerle, bu bitki ümit verici
antioksidan ajan olarak kabul edilebilir.

Keywords

Galanthus woronowii, doğal ürün, antioksidan aktivite, Fenolik içerik

References

Aksit H, Çelik SM, Sen Ö, Erenler R, Demirtas I, Telci I, Elmastas M (2014). Complete isolation and characterization of polar portion of Mentha dumetorum water extract. Records of Natural Products 8: 277-280.
Apak R, Güçlü K, Özyürek M, Karademir SE (2004). Novel total antioxidant capacity index for dietary polyphenols and vitamins C and E, using their cupric ion reducing capability in the presence of neocuproine: CUPRAC method. Journal of Agricultural and Food Chemistry 52: 7970-7981.
Blois MS (1958). Antioxidant determinations by the use of a stable free radical. Nature 181: 1199-1200.
Bokov D, Samylina I (2016). Identification of Flavonoids in Homeopathic Mother Tinctures of Galanthus woronowii Losinsk. And Galanthus nivalis L. by Ultra-Performance Liquid Chromatography with Photodiode Arrays and Tandem Quadrupole Mass-Selective Detectors. Pharmaceutical Chemistry Journal 50:458-464.
Chhikara N, Kushwaha K, Sharma P, Gat Y, Panghal A (2018). Bioactive compounds of beetroot and utilization in food processing industry: A critical review. Food Chemistry 272: 192-200.
Davis AP (2006). The Genus Galanthus-snowdrops in the wild. In: Snowdrops, A Monograph of Cultivated Galanthus. Cheltenham: Griffin Press Publishing Ltd. pp. 9-63.
Demirtas I, Erenler R, Elmastas M, Goktasoglu A (2013). Studies on the antioxidant potential of flavones of Allium vineale isolated from its water-soluble fraction. Food Chemistry 136: 34-40.
Elmastas M, Celik SM, Genc N, Aksit H, Erenler R, Gulcin İ (2018). Antioxidant activity of an Anatolian herbal tea—Origanum minutiflorum: isolation and characterization of its secondary metabolites. International Journal of Food Properties 21: 374-384.
Elmastas M, Erenler R, Isnac B, Aksit H, Sen O, Genc N, Demirtas I (2016). Isolation and identification of a new neo-clerodane diterpenoid from Teucrium chamaedrys L. Natural Product Research 30: 299-304.
Elmastas M, Ozturk L, Gokce I, Erenler R, Aboul-Enein HY (2004). Determination of antioxidant activity of marshmallow flower (Althaea officinalis L.). Analytical Letters 37: 1859-1869.
Elmastaş M, Telci İ, Akşit H, Erenler R (2015). Comparison of total phenolic contents and antioxidant capacities in mint genotypes used as spices/Baharat olarak kullanılan nane genotiplerinin toplam fenolik içerikleri ve antioksidan kapasitelerinin karşılaştırılması. Turkish Journal of Biochemistry 40: 456-462.
Erenler R, Adak T, Karan T, Elmastas M, Yildiz I, Aksit H, Topcu G, Sanda MA (2017). Chemical Constituents isolated from Origanum solymicum with Antioxidant activities. The Eurasia Proceedings of Science, Technology, Engineering & Mathematics 1: 139-145.
Erenler R, Demirtas I, Karan T, Altun M, Gul F (2017). Inhibitory Effect of 6, 7-dimethoxy-5-hydroxyflavone on Human Cervix Carcinoma in Vitro. International Journal of Secondary Metabolite (IJSM) 4: 512-516.
Erenler R, Meral B, Sen O, Elmastas M, Aydin A, Eminagaoglu O, Topcu G (2017). Bioassay-guided isolation, identification of compounds from Origanum rotundifolium and investigation of their antiproliferative and antioxidant activities. Pharmaceutical Biology 55: 1646-1653.
Erenler R, Pabuccu K, Yaglioglu AS, Demirtas I, Gul F (2016). Chemical constituents and antiproliferative effects of cultured Mougeotia nummuloides and Spirulina major against cancerous cell lines. Zeitschrift Fur Naturforschung Section C- Journal of Biosciences 71: 87-92.
Erenler R, Sen O, Aksit H, Demirtas I, Yaglioglu AS, Elmastas M, Telci İ (2016). Isolation and identification of chemical constituents from Origanum majorana and investigation of antiproliferative and antioxidant activities. Journal of the Science of Food and Agriculture 96: 822-836.
Erenler R, Sen O, Yaglioglu AS, Demirtas I (2016). Bioactivity-guided isolation of antiproliferative sesquiterpene lactones from Centaurea solstitialis L. ssp. solstitialis. Comb Chem High Throughput Screen 19: 66-72.
Erenler R, Sen O, Yildiz I, Aydin A (2016). Antiproliferative activities of chemical constituents isolated from Thymus praecox subsp grossheimii (Ronniger) Jalas. Records of Natural Products 10: 766-770.
Erenler R, Telci İ, Elmastaş M, Akşit H, Gül F, Tüfekçi AR, Demirtaş İ, Kayir Ö (2018). Quantification of flavonoids isolated from Mentha spicata in selected clones of Turkish mint landraces. Turkish Journal of Chemistry 42: 1695-1705.
Erenler R, Telci I, Ulutas M, Demirtas I, Gul F, Elmastas M, Kayir O (2015). Chemical Constituents, Quantitative Analysis and Antioxidant Activities of Echinacea purpurea (L.) Moench and Echinacea pallida (Nutt.) Nutt. Journal of Food Biochemistry 39: 622-630.
Erenler R, Yilmaz S, Aksit H, Sen O, Genc N, Elmastas M, Demirtas I (2014). Antioxidant activities of chemical constituents isolated from Echinops orientalis Trauv. Records of Natural Products 8 (1): 32-36.
Guzel A, Aksit H, Elmastas M, Erenler R (2017). Bioassay-guided isolation and identification of antioxidant flavonoids from Cyclotrichium origanifolium (Labill.) Manden. and Scheng. Pharmacognosy Magazine 13 (50): 316-320.
Howes M-JR, Perry E (2011). The role of phytochemicals in the treatment and prevention of dementia. Drugs & aging 28: 439-468.
Jin Z (2013). Amaryllidaceae and Sceletium alkaloids. Natural Product Reports 30: 849-868.
Karan T, Altuner Z, Erenler R (2017). Growth and Metabolite Production of Chroococcus minutus Under Different Temperature and Light Conditions. Journal of New Results in Science 6: 47-52.
Karan T, Erenler R (2018). Fatty acid constituents and anticancer activity of Cladophora fracta (OF Müller ex Vahl) Kützing. Tropical Journal of Pharmaceutical Research 17: 1977-1982.
Karan T, Erenler R (2017). Screening of norharmane from seven cyanobacteria by high-performance liquid chromatography. Pharmacognosy Magazine 13: 723-725.
Karan T, Yildiz I, Aydin A, Erenler R (2018). Inhibition of various cancer cells proliferation of bornyl acetate and essential oil from Inula graveolens (Linnaeus) Desf. Records of Natural Products 12: 273-283.
Kintsurashvili L, Vachnadze V (2007). Plants of the Amaryllidaceae family grown and introduced in Georgia: a source of galanthamine. Pharmaceutical Chemistry Journal 41: 492-494.
Koysu P, Genc N, Elmastas M, Aksit H, Erenler R (2018). Isolation, identification of secondary metabolites from Salvia absconditiflora and evaluation of their antioxidative properties. Natural Product Research 16: 1-4.
Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine 26: 1231-1237.
Sarikaya BB, Kaya GI, Onur MA, Bastida J, Somer NU (2013). Phytochemical investigation of Galanthus woronowii. Biochemical Systematics and Ecology 51: 276-279.
Sasaki YF, Kawaguchi S, Kamaya A, Ohshita M, Kabasawa K, Iwama K, Taniguchi K, Tsuda S (2002). The comet assay with 8 mouse organs: results with 39 currently used food additives. Mutation Research/Genetic Toxicology and Environmental Mutagenesis 519: 103-119.
Singleton VL, Slinkard K (1977). Total phenol analysis: Automation and comparison with manual methods. American Journal of Enology and Viticulture 28: 49-55.
Topçu G, Erenler R, Çakmak O, Johansson CB, Çelik C, Chai H-B, Pezzuto JM (1999). Diterpenes from the berries of Juniperus excelsa. Phytochemistry 50: 1195-1199.
Yaglıoglu AS, Akdulum B, Erenler R, Demirtas I, Telci I, Tekin S (2013). Antiproliferative activity of pentadeca-(8E, 13Z) dien-11-yn-2-one and (E)-1, 8-pentadecadiene from Echinacea pallida (Nutt.) Nutt. roots. Medicinal Chemistry Research 22: 2946-2953.

Antioxidant activity and total phenolic contents of Galanthus woronowii (Amaryllidaceae).

Year 2019, Volume: 2 Issue: 1, 1 - 5, 31.03.2019

Nusret Genç , İlyas Yıldız Tunay Karan Özgür Eminağaoğlu Ramazan Erenler

https://doi.org/10.38059/biodiversity.515111

Cited By: 20

Abstract

Plants
have been used for medicinal purpose since ancient times. Due to the including
bioactive secondary metabolites, plants have gained the great interest for drug
discovery and development process. In this work, Galanthus woronowii was extracted with hexane, dichloromethane and
ethyl acetate sequentially. After removing of the solvent by rotary evaporator, crude extracts were yielded. Antioxidant activity including 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic
acid) (ABTS) and reducing power assays were executed on corresponding extracts. In addition, total phenolic content was presented. Ethyl acetate extract
included the most phenolic compounds and also it revealed the most antioxidant
activity. Hence, this plant could be considered as a promising antioxidant
agent.

Keywords

Galanthus woronowii, antioxidant activity, phenolic contents, natural product

References

Aksit H, Çelik SM, Sen Ö, Erenler R, Demirtas I, Telci I, Elmastas M (2014). Complete isolation and characterization of polar portion of Mentha dumetorum water extract. Records of Natural Products 8: 277-280.
Apak R, Güçlü K, Özyürek M, Karademir SE (2004). Novel total antioxidant capacity index for dietary polyphenols and vitamins C and E, using their cupric ion reducing capability in the presence of neocuproine: CUPRAC method. Journal of Agricultural and Food Chemistry 52: 7970-7981.
Blois MS (1958). Antioxidant determinations by the use of a stable free radical. Nature 181: 1199-1200.
Bokov D, Samylina I (2016). Identification of Flavonoids in Homeopathic Mother Tinctures of Galanthus woronowii Losinsk. And Galanthus nivalis L. by Ultra-Performance Liquid Chromatography with Photodiode Arrays and Tandem Quadrupole Mass-Selective Detectors. Pharmaceutical Chemistry Journal 50:458-464.
Chhikara N, Kushwaha K, Sharma P, Gat Y, Panghal A (2018). Bioactive compounds of beetroot and utilization in food processing industry: A critical review. Food Chemistry 272: 192-200.
Davis AP (2006). The Genus Galanthus-snowdrops in the wild. In: Snowdrops, A Monograph of Cultivated Galanthus. Cheltenham: Griffin Press Publishing Ltd. pp. 9-63.
Demirtas I, Erenler R, Elmastas M, Goktasoglu A (2013). Studies on the antioxidant potential of flavones of Allium vineale isolated from its water-soluble fraction. Food Chemistry 136: 34-40.
Elmastas M, Celik SM, Genc N, Aksit H, Erenler R, Gulcin İ (2018). Antioxidant activity of an Anatolian herbal tea—Origanum minutiflorum: isolation and characterization of its secondary metabolites. International Journal of Food Properties 21: 374-384.
Elmastas M, Erenler R, Isnac B, Aksit H, Sen O, Genc N, Demirtas I (2016). Isolation and identification of a new neo-clerodane diterpenoid from Teucrium chamaedrys L. Natural Product Research 30: 299-304.
Elmastas M, Ozturk L, Gokce I, Erenler R, Aboul-Enein HY (2004). Determination of antioxidant activity of marshmallow flower (Althaea officinalis L.). Analytical Letters 37: 1859-1869.
Elmastaş M, Telci İ, Akşit H, Erenler R (2015). Comparison of total phenolic contents and antioxidant capacities in mint genotypes used as spices/Baharat olarak kullanılan nane genotiplerinin toplam fenolik içerikleri ve antioksidan kapasitelerinin karşılaştırılması. Turkish Journal of Biochemistry 40: 456-462.
Erenler R, Adak T, Karan T, Elmastas M, Yildiz I, Aksit H, Topcu G, Sanda MA (2017). Chemical Constituents isolated from Origanum solymicum with Antioxidant activities. The Eurasia Proceedings of Science, Technology, Engineering & Mathematics 1: 139-145.
Erenler R, Demirtas I, Karan T, Altun M, Gul F (2017). Inhibitory Effect of 6, 7-dimethoxy-5-hydroxyflavone on Human Cervix Carcinoma in Vitro. International Journal of Secondary Metabolite (IJSM) 4: 512-516.
Erenler R, Meral B, Sen O, Elmastas M, Aydin A, Eminagaoglu O, Topcu G (2017). Bioassay-guided isolation, identification of compounds from Origanum rotundifolium and investigation of their antiproliferative and antioxidant activities. Pharmaceutical Biology 55: 1646-1653.
Erenler R, Pabuccu K, Yaglioglu AS, Demirtas I, Gul F (2016). Chemical constituents and antiproliferative effects of cultured Mougeotia nummuloides and Spirulina major against cancerous cell lines. Zeitschrift Fur Naturforschung Section C- Journal of Biosciences 71: 87-92.
Erenler R, Sen O, Aksit H, Demirtas I, Yaglioglu AS, Elmastas M, Telci İ (2016). Isolation and identification of chemical constituents from Origanum majorana and investigation of antiproliferative and antioxidant activities. Journal of the Science of Food and Agriculture 96: 822-836.
Erenler R, Sen O, Yaglioglu AS, Demirtas I (2016). Bioactivity-guided isolation of antiproliferative sesquiterpene lactones from Centaurea solstitialis L. ssp. solstitialis. Comb Chem High Throughput Screen 19: 66-72.
Erenler R, Sen O, Yildiz I, Aydin A (2016). Antiproliferative activities of chemical constituents isolated from Thymus praecox subsp grossheimii (Ronniger) Jalas. Records of Natural Products 10: 766-770.
Erenler R, Telci İ, Elmastaş M, Akşit H, Gül F, Tüfekçi AR, Demirtaş İ, Kayir Ö (2018). Quantification of flavonoids isolated from Mentha spicata in selected clones of Turkish mint landraces. Turkish Journal of Chemistry 42: 1695-1705.
Erenler R, Telci I, Ulutas M, Demirtas I, Gul F, Elmastas M, Kayir O (2015). Chemical Constituents, Quantitative Analysis and Antioxidant Activities of Echinacea purpurea (L.) Moench and Echinacea pallida (Nutt.) Nutt. Journal of Food Biochemistry 39: 622-630.
Erenler R, Yilmaz S, Aksit H, Sen O, Genc N, Elmastas M, Demirtas I (2014). Antioxidant activities of chemical constituents isolated from Echinops orientalis Trauv. Records of Natural Products 8 (1): 32-36.
Guzel A, Aksit H, Elmastas M, Erenler R (2017). Bioassay-guided isolation and identification of antioxidant flavonoids from Cyclotrichium origanifolium (Labill.) Manden. and Scheng. Pharmacognosy Magazine 13 (50): 316-320.
Howes M-JR, Perry E (2011). The role of phytochemicals in the treatment and prevention of dementia. Drugs & aging 28: 439-468.
Jin Z (2013). Amaryllidaceae and Sceletium alkaloids. Natural Product Reports 30: 849-868.
Karan T, Altuner Z, Erenler R (2017). Growth and Metabolite Production of Chroococcus minutus Under Different Temperature and Light Conditions. Journal of New Results in Science 6: 47-52.
Karan T, Erenler R (2018). Fatty acid constituents and anticancer activity of Cladophora fracta (OF Müller ex Vahl) Kützing. Tropical Journal of Pharmaceutical Research 17: 1977-1982.
Karan T, Erenler R (2017). Screening of norharmane from seven cyanobacteria by high-performance liquid chromatography. Pharmacognosy Magazine 13: 723-725.
Karan T, Yildiz I, Aydin A, Erenler R (2018). Inhibition of various cancer cells proliferation of bornyl acetate and essential oil from Inula graveolens (Linnaeus) Desf. Records of Natural Products 12: 273-283.
Kintsurashvili L, Vachnadze V (2007). Plants of the Amaryllidaceae family grown and introduced in Georgia: a source of galanthamine. Pharmaceutical Chemistry Journal 41: 492-494.
Koysu P, Genc N, Elmastas M, Aksit H, Erenler R (2018). Isolation, identification of secondary metabolites from Salvia absconditiflora and evaluation of their antioxidative properties. Natural Product Research 16: 1-4.
Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine 26: 1231-1237.
Sarikaya BB, Kaya GI, Onur MA, Bastida J, Somer NU (2013). Phytochemical investigation of Galanthus woronowii. Biochemical Systematics and Ecology 51: 276-279.
Sasaki YF, Kawaguchi S, Kamaya A, Ohshita M, Kabasawa K, Iwama K, Taniguchi K, Tsuda S (2002). The comet assay with 8 mouse organs: results with 39 currently used food additives. Mutation Research/Genetic Toxicology and Environmental Mutagenesis 519: 103-119.
Singleton VL, Slinkard K (1977). Total phenol analysis: Automation and comparison with manual methods. American Journal of Enology and Viticulture 28: 49-55.
Topçu G, Erenler R, Çakmak O, Johansson CB, Çelik C, Chai H-B, Pezzuto JM (1999). Diterpenes from the berries of Juniperus excelsa. Phytochemistry 50: 1195-1199.
Yaglıoglu AS, Akdulum B, Erenler R, Demirtas I, Telci I, Tekin S (2013). Antiproliferative activity of pentadeca-(8E, 13Z) dien-11-yn-2-one and (E)-1, 8-pentadecadiene from Echinacea pallida (Nutt.) Nutt. roots. Medicinal Chemistry Research 22: 2946-2953.

There are 36 citations in total.

Details

Primary Language	English
Journal Section	Research Articles
Authors	Nusret Genç 0000-0003-3685-1344 İlyas Yıldız 0000-0003-1254-1069 Tunay Karan 0000-0002-9114-8400 Özgür Eminağaoğlu 0000-0003-0064-0318 Ramazan Erenler 0000-0002-0505-3190
Publication Date	March 31, 2019
Submission Date	January 20, 2019
Acceptance Date	January 31, 2019
Published in Issue	Year 2019 Volume: 2 Issue: 1

Cite

APA	Genç, N., Yıldız, İ., Karan, T., Eminağaoğlu, Ö., et al. (2019). Antioxidant activity and total phenolic contents of Galanthus woronowii (Amaryllidaceae). Turkish Journal of Biodiversity, 2(1), 1-5. https://doi.org/10.38059/biodiversity.515111