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Origanum rotundifolium’un endofitik bakteri çeşitliliği: izolasyon ve moleküler tanımlama

Year 2025, Volume: 8 Issue: 1, 11 - 16, 30.06.2025

Nurcan Albayrak İskender

https://doi.org/10.38059/biodiversity.1704823

Abstract

Tıbbi ve aromatik bitkilerdeki endofitik bakteriler, bu bitkilerin mikrobiyal çeşitliliği hakkında değerli bilgiler sunmakta ve ekolojik rollerinin yanı sıra biyoteknolojik potansiyellerine dair araştırmalar için temel oluşturmaktadır. Bu çalışmada, Origanum rotundifolium Boiss.’un endofitik bakteri çeşitliliği belirlenmiş ve toplamda on bir endofitik bakteri, O. rotundifolium’un kök, gövde ve yaprak dokularından izole edilmiştir. İzole edilen bakteriler, bazı morfolojik ve biyokimyasal özelliklerine göre karakterize edilmiştir. Moleküler tanımlama, 16S rRNA gen dizileme yöntemiyle yapılmıştır. Tanımlanan izolatlar sırasıyla: Kocuria rosea (OR1), Schumannella sp. (OR2), Micrococcus luteus (OR3, OR7), Bacillus proteolyticus (OR4), Curtobacterium flaccumfaciens (OR5), Staphylococcus sp. (OR6, OR10), Microbacterium proteolyticum (OR8), Microbacterium sp. (OR9) ve Actinomyces sp. (OR11) olarak belirlenmiştir. Bu çalışma, Origanum rotundifolium'dan izole edilen endofitik bakteri çeşitliliğini ilk kez rapor etmektedir.

Keywords

Origanum rotundifolium Endofitik bakteri 16S rRNA Moleküler tanımlama Tıbbi aromatik bitki

Project Number

ACU-BAP: 2021.M80.02.02

References

  • Arora NK, Khare E, Singh S, Tewari S (2018). Phenetic, genetic diversity and symbiotic compatibility of rhizobial strains nodulating pigeon pea in Northern India. 3 Biotech 8(1): 1–10.
  • Baser KHC, Özek T, Tümen G (1995). Essential oil of Origanum rotundifolium Boiss. Journal of Essential Oil Research 7(1): 95–96. DOI: 10.1080/10412905.1995.9698474.
  • Baydar H, Osman S, Özkan G, Karadoğan T (2004). Antibacterial activity and composition of essential oils from Origanum, Thymbra and Satureja species with commercial importance in Turkey. Food Control 15(3): 169–172. DOI: 10.1016/S0956-7135(03)00028-8.
  • Başer KHC (2008). Biological and pharmacological activities of carvacrol and carvacrol bearing essential oils. Current Pharmaceutical Design 14(29): 3106–3119.
  • Castronovo LM, Calonico C, Ascrizzi R, Del Duca S, Delfino V, Chioccioli S, Bianchi A, Toccaceli M, Guidi C, Fani R (2020). The cultivable bacterial microbiota associated to the medicinal plant Origanum vulgare L.: From antibiotic resistance to growth-inhibitory properties. Frontiers in Microbiology 11: 862.
  • Chishti S, Kaloo ZA, Sultan P (2013). Medicinal importance of genus Origanum: A review. Journal of Pharmacognosy and Phytotherapy 5(9): 170–177.
  • Davis PH (1982). Flora of Turkey and the East Aegean Islands, Vol. 7, Edinburgh: Edinburgh University Press.
  • Dikbaş N, Kotan R, Dadaşoğlu F, Karagöz K, Çakmakcı R (2009). Correlation between major constituents and antibacterial activities of some plant essential oils against some pathogenic bacteria. Turkish Journal of Science & Technology 4(2): 57–64.
  • Fleisher A, Fleisher Z (1988). Identification of biblical hyssop and origin of the traditional use of oregano-group herbs in the Mediterranean region. Economic Botany 42(2): 232–241.
  • Goze I, Alim A, Tepe AS, Sökmen M, Sevgi K, Tepe B (2009). Screening of the antioxidant activity of essential oil and various extracts of Origanum rotundifolium Boiss. from Turkey. Journal of Medicinal Plants Research 3(4): 246–254.
  • Görmez A, Bozari S, Yanmış D, Güllüce M, Ağar G, Şahin F (2016). The use of essential oils of Origanum rotundifolium as antimicrobial agent against plant pathogenic bacteria. Journal of Essential Oil Bearing Plants 19(3): 656–663. DOI: 10.1080/0972060X.2014.935052.
  • Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program forWindows 95/98/NT. Nucleic Acids Symposium Series 41: 95-98.
  • Harley JP, Prescott LM (2002). Laboratory Exercises in Microbiology, 5th ed.; McGraw-Hill: New York, 466 pp. Kulisic T, Radonic A, Katalinic V, Milos M (2004). Use of different methods for testing antioxidative activity of oregano essential oil. Food Chemistry 85(4): 633–640. DOI: 10.1016/j.foodchem.2003.07.014.
  • Mohamad OAA, Li L, Ma JB, Hatab S, Xu L, Guo JW, Rasulov BA, Liu YH, Hedlund BP, Li WJ (2018). Evaluation of the antimicrobial activity of endophytic bacterial populations from Chinese traditional medicinal plant licorice and characterization of the bioactive secondary metabolites produced by Bacillus atrophaeus against Verticillium dahliae. Frontiers in Microbiology 9: 924.
  • Nohutçu L, Tunçtürk M, Şelem E, Tunçtürk R, Çınar O (2024). Determination of nutrient elements content, essential oils ratio and biochemicals composition of Origanum rotundifolium Boiss. and Origanum syriacum L. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 27(Ek Sayı 1, Suppl 1), 213-230.
  • Polito G, Semenzato G, Del Duca S, Castronovo LM, Vassallo A, Chioccioli S, Borsetti D, Calabretta V, Puglia AM, Fani R, Palumbo Piccionello A (2022). Endophytic bacteria and essential oil from Origanum vulgare ssp. vulgare share some VOCs with an antibacterial activity. Microorganisms 10(7): 1424.
  • POWO (Plants of the World Online) (2025). Origanum rotundifolium Boiss. http://powo.science.kew.org/taxon/urn:lsid:ipni.org:names:453350-1. Accessed: May 19, 2025.
  • Sahu P.K., Tilgam J., Mishra S., Hamid S., Gupta A., Jayalakshmi K., Verma S.K., Kharwar R.N. (2022). Surface sterilization for isolation of endophytes: Ensuring what (not) to grow. Journal of Basic Microbiology 62(6), 647–668.
  • Semenzato G, Faddetta T, Falsini S, Del Duca S, Esposito A, Padula A, Bianchi A, Flamini G, Guidi C, Fani R (2022). Endophytic bacteria associated with Origanum heracleoticum L. (Lamiaceae) seeds. Microorganisms 10(10): 2086.
  • Tamura K, Stecher G, and Kumar S (2021) MEGA11: Molecular Evolutionary Genetics Analysis version 11. Molecular Biology and Evolution 38: 3022-3027.
  • Vitali F, Frascella A, Semenzato G, Del Duca S, Palumbo Piccionello A, Mocali S, Flamini G, Macchia M, Castronovo LM, Fani R (2023). Employing genome mining to unveil a potential contribution of endophytic bacteria to antimicrobial compounds in the Origanum vulgare L. essential oil. Antibiotics 12(7): 1179.
  • Yıldırım E, Kordalı S, Yazıcı G (2011). Insecticidal effects of essential oils of eleven plant species from Lamiaceae on Sitophilus granarius (L.) (Coleoptera: Curculionidae). Biotechnology Letters 16(5): 6702–6709.

Endophytic bacterial diversity of Origanum rotundifolium: isolation and molecular identification of strains

Year 2025, Volume: 8 Issue: 1, 11 - 16, 30.06.2025

Nurcan Albayrak İskender

https://doi.org/10.38059/biodiversity.1704823

Abstract

Endophytic bacteria in medicinal and aromatic plants provide valuable insights into the microbial diversity of these plants and form the basis for research on their ecological roles and biotechnological potential. In this study, the endophytic bacterial diversity of Origanum rotundifolium Boiss. was determined, and a total of eleven endophytic bacteria were isolated from the root, stem, and leaf tissues of O. rotundifolium. The isolated bacteria were characterized based on certain morphological and biochemical properties. Molecular identification was performed using 16S rRNA gene sequencing. The identified isolates were as follows: Kocuria rosea (OR1), Schumannella sp. (OR2), Micrococcus luteus (OR3, OR7), Bacillus proteolyticus (OR4), Curtobacterium flaccumfaciens (OR5), Staphylococcus sp. (OR6, OR10), Microbacterium proteolyticum (OR8), Microbacterium sp. (OR9), and Actinomyces sp. (OR11). This study reports, for the first time, the diversity of endophytic bacteria isolated from Origanum rotundifolium.

Keywords

Origanum rotundifolium Endophytic bacteria 16S rRNA gene sequencing Molecular identification Medicinal and aromatic plants

Ethical Statement

The study involved only plant material and did not require ethical approval.

Supporting Institution

This work was supported by Artvin Çoruh University Research Foundation

Project Number

ACU-BAP: 2021.M80.02.02

Thanks

I would like to thank Prof. Dr. Melahat ÖZCAN for her contribution to the collection of plant samples and identification of the species.

References

  • Arora NK, Khare E, Singh S, Tewari S (2018). Phenetic, genetic diversity and symbiotic compatibility of rhizobial strains nodulating pigeon pea in Northern India. 3 Biotech 8(1): 1–10.
  • Baser KHC, Özek T, Tümen G (1995). Essential oil of Origanum rotundifolium Boiss. Journal of Essential Oil Research 7(1): 95–96. DOI: 10.1080/10412905.1995.9698474.
  • Baydar H, Osman S, Özkan G, Karadoğan T (2004). Antibacterial activity and composition of essential oils from Origanum, Thymbra and Satureja species with commercial importance in Turkey. Food Control 15(3): 169–172. DOI: 10.1016/S0956-7135(03)00028-8.
  • Başer KHC (2008). Biological and pharmacological activities of carvacrol and carvacrol bearing essential oils. Current Pharmaceutical Design 14(29): 3106–3119.
  • Castronovo LM, Calonico C, Ascrizzi R, Del Duca S, Delfino V, Chioccioli S, Bianchi A, Toccaceli M, Guidi C, Fani R (2020). The cultivable bacterial microbiota associated to the medicinal plant Origanum vulgare L.: From antibiotic resistance to growth-inhibitory properties. Frontiers in Microbiology 11: 862.
  • Chishti S, Kaloo ZA, Sultan P (2013). Medicinal importance of genus Origanum: A review. Journal of Pharmacognosy and Phytotherapy 5(9): 170–177.
  • Davis PH (1982). Flora of Turkey and the East Aegean Islands, Vol. 7, Edinburgh: Edinburgh University Press.
  • Dikbaş N, Kotan R, Dadaşoğlu F, Karagöz K, Çakmakcı R (2009). Correlation between major constituents and antibacterial activities of some plant essential oils against some pathogenic bacteria. Turkish Journal of Science & Technology 4(2): 57–64.
  • Fleisher A, Fleisher Z (1988). Identification of biblical hyssop and origin of the traditional use of oregano-group herbs in the Mediterranean region. Economic Botany 42(2): 232–241.
  • Goze I, Alim A, Tepe AS, Sökmen M, Sevgi K, Tepe B (2009). Screening of the antioxidant activity of essential oil and various extracts of Origanum rotundifolium Boiss. from Turkey. Journal of Medicinal Plants Research 3(4): 246–254.
  • Görmez A, Bozari S, Yanmış D, Güllüce M, Ağar G, Şahin F (2016). The use of essential oils of Origanum rotundifolium as antimicrobial agent against plant pathogenic bacteria. Journal of Essential Oil Bearing Plants 19(3): 656–663. DOI: 10.1080/0972060X.2014.935052.
  • Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program forWindows 95/98/NT. Nucleic Acids Symposium Series 41: 95-98.
  • Harley JP, Prescott LM (2002). Laboratory Exercises in Microbiology, 5th ed.; McGraw-Hill: New York, 466 pp. Kulisic T, Radonic A, Katalinic V, Milos M (2004). Use of different methods for testing antioxidative activity of oregano essential oil. Food Chemistry 85(4): 633–640. DOI: 10.1016/j.foodchem.2003.07.014.
  • Mohamad OAA, Li L, Ma JB, Hatab S, Xu L, Guo JW, Rasulov BA, Liu YH, Hedlund BP, Li WJ (2018). Evaluation of the antimicrobial activity of endophytic bacterial populations from Chinese traditional medicinal plant licorice and characterization of the bioactive secondary metabolites produced by Bacillus atrophaeus against Verticillium dahliae. Frontiers in Microbiology 9: 924.
  • Nohutçu L, Tunçtürk M, Şelem E, Tunçtürk R, Çınar O (2024). Determination of nutrient elements content, essential oils ratio and biochemicals composition of Origanum rotundifolium Boiss. and Origanum syriacum L. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 27(Ek Sayı 1, Suppl 1), 213-230.
  • Polito G, Semenzato G, Del Duca S, Castronovo LM, Vassallo A, Chioccioli S, Borsetti D, Calabretta V, Puglia AM, Fani R, Palumbo Piccionello A (2022). Endophytic bacteria and essential oil from Origanum vulgare ssp. vulgare share some VOCs with an antibacterial activity. Microorganisms 10(7): 1424.
  • POWO (Plants of the World Online) (2025). Origanum rotundifolium Boiss. http://powo.science.kew.org/taxon/urn:lsid:ipni.org:names:453350-1. Accessed: May 19, 2025.
  • Sahu P.K., Tilgam J., Mishra S., Hamid S., Gupta A., Jayalakshmi K., Verma S.K., Kharwar R.N. (2022). Surface sterilization for isolation of endophytes: Ensuring what (not) to grow. Journal of Basic Microbiology 62(6), 647–668.
  • Semenzato G, Faddetta T, Falsini S, Del Duca S, Esposito A, Padula A, Bianchi A, Flamini G, Guidi C, Fani R (2022). Endophytic bacteria associated with Origanum heracleoticum L. (Lamiaceae) seeds. Microorganisms 10(10): 2086.
  • Tamura K, Stecher G, and Kumar S (2021) MEGA11: Molecular Evolutionary Genetics Analysis version 11. Molecular Biology and Evolution 38: 3022-3027.
  • Vitali F, Frascella A, Semenzato G, Del Duca S, Palumbo Piccionello A, Mocali S, Flamini G, Macchia M, Castronovo LM, Fani R (2023). Employing genome mining to unveil a potential contribution of endophytic bacteria to antimicrobial compounds in the Origanum vulgare L. essential oil. Antibiotics 12(7): 1179.
  • Yıldırım E, Kordalı S, Yazıcı G (2011). Insecticidal effects of essential oils of eleven plant species from Lamiaceae on Sitophilus granarius (L.) (Coleoptera: Curculionidae). Biotechnology Letters 16(5): 6702–6709.
There are 22 citations in total.

Details

Primary Language English
Subjects Plant Biotechnology
Journal Section Research Articles
Authors

Nurcan Albayrak İskender 0000-0001-8413-3190

Project Number ACU-BAP: 2021.M80.02.02
Early Pub Date June 27, 2025
Publication Date June 30, 2025
Submission Date May 23, 2025
Acceptance Date June 1, 2025
Published in Issue Year 2025 Volume: 8 Issue: 1

Cite

APA Albayrak İskender, N. (2025). Endophytic bacterial diversity of Origanum rotundifolium: isolation and molecular identification of strains. Turkish Journal of Biodiversity, 8(1), 11-16. https://doi.org/10.38059/biodiversity.1704823
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