Abstract
Amaç: İnvazyon, embriyonun uterus epiteline implantasyonu gibi normal gelişim sürecinde olabildiği gibi kanser hücrelerinin yayılması gibi patolojik olaylarda da görülmektedir. Kanser hücrelerinin invazyon mekanizmalarını anlayabilmek için, hücreleri in vivo ortamlarına yakın bir ortamda araştırmak gerekmektedir. Bu çalışmanın amacı, kanser hücrelerinin invazyon araştırmalarında kullanılabilecek in vivo ortamı taklit eden yeni bir in vitro kültür ortamının geliştirilmesidir.
Gereç ve Yöntem: İnsan metastatik meme adenokarsinom (M4A4) ve insan primer kolon karsinoma (HCT-116) hücre dizileri kültüre edilmiştir. İki farklı biyouyumlu doku iskelesi amacıyla bakteriyal selüloz ve kollajen köpük kullanılmıştır. Hücreler doku iskelelerine ekildikten sonra 3D kanser invazyon kültür modelleri oluşturulmuştur. Hücre morfolojisi değerlendirilmiş, Hematoksilen-Eozin boyasıyla histolojik incelemeler yapılmıştır.
Bulgular: M4A4 hücreleri, bakteriyel selüloz iskelelerinde fibroblastik morfolojiyi korurken, kollajen köpük iskelelerinde kümelenip çoğalmıştır. HCT-116 hücreleri ise bakteriyel selüloz iskelelerinde yoğun kümelenme ve çoğalma, kollajen köpük iskelelerinde ise fibroblastik morfoloji sergilemiştir. Histolojik incelemeler, HCT-116 hücrelerinin bakteriyel selüloz iskelelerinde, M4A4 hücrelerinin ise kollajen köpük iskelelerinde daha yüksek invazyon ve koloni oluşumu gösterdiğini ortaya koymuştur.
Sonuç: Çalışmanın bulguları, iskele seçiminin kanser araştırmalarında hücre davranışı ve çoğalması üzerindeki önemli etkisini vurgulamaktadır. Histolojik sonuçlar, bakteriyel selüloz ve kollajen köpük iskelelerinin farklı kanser hücre hatlarıyla etkileşimlerini ve invaziv davranışlarını etkilediğini göstermektedir. Bu bilgiler, kanser hücresi etkileşimlerinin ve davranışlarının karmaşıklığını anlamak ve incelemek için özel iskele seçimine duyulan ihtiyacı vurgulamaktadır.
Ethical Statement
İnsan metastatik meme adenokarsinom (M4A4, CRL-2914) ve insan primer kolon karsinoma (HCT-116, CCL-247) hücre dizileri ticari olarak ATCC'den satın alınmıştır ve kullanım için herhangi bir etik onay gerektirmemektedir.
References
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- 3. Amiri FZ, Pashandi Z, Lotfibakhshaiesh N, Mirzaei-Parsa MJ, Ghanbari H,Faridi‐Majidi R. Cell Attachment Effects of Collagen Nanoparticles on Crosslinked Electrospun Nanofibers. The International Journal of Artificial Organs. 2020;44(3):199-207.
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- 5. Renó CO, Lima BFAS, Sousa Ed, Bertran CA,Motisuke M. Scaffolds of Calcium Phosphate Cement Containing Chitosan and Gelatin. Materials Research. 2013;16(6):1362-5.
- 6. Beran M, Berkova E, MusÍLkovÁ J, Sedlář A, Slepička P,Fajstavr D. Mineralised Polylactide and Polycaprolactone Soft Foams With Hierarchical Micro-Macro Porous Structure for Tissue Engineering. 2021.
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Abstract
Aim: Invasion can occur in normal developmental processes such as implantation of the embryo into the uterine epithelium, but also in pathological events such as the spread of cancer cells. In order to understand the mechanisms of invasion of cancer cells, it is necessary to investigate the cells in an environment close to their in vivo environment. The aim of this study is to develop a new in vitro culture medium that mimics the in vivo environment that can be used in cancer cell invasion studies.
Method: Human metastatic breast adenocarcinoma (M4A4) and human primary colon carcinoma (HCT-116) cell lines were cultured. Bacterial cellulose and collagen foam were used to produce two different biocompatible tissue scaffolds. Cells were seeded onto these scaffolds and 3D cancer invasion culture models were created. Cell morphology was evaluated and histological examinations were performed with Hematoxylin-Eosin stain.
Results: M4A4 cells retained fibroblastic morphology in bacterial cellulose scaffolds, but clustered and proliferated in collagen foam scaffolds. HCT-116 cells exhibited dense clustering and proliferation in bacterial cellulose scaffolds and fibroblastic morphology in collagen foam scaffolds. Histological examinations revealed that HCT-116 cells showed higher invasion and colony formation in bacterial cellulose scaffolds and M4A4 cells showed higher invasion and colony formation in collagen foam scaffolds.
Conclusion: The findings of the study highlight the important impact of scaffold selection on cell behaviour and proliferation in cancer research. Histological results show that bacterial cellulose and collagen foam scaffolds affect interactions with different cancer cell lines and their invasive behaviour. This information highlights the need for specific scaffold selection to understand and study the complexity of cancer cell interactions and behaviours.
Ethical Statement
Human metastatic breast adenocarcinoma (M4A4, CRL-2914) and human primary colon carcinoma (HCT-116, CCL-247) cell lines were purchased commercially from ATCC and do not require any ethical approval for use.
References
- 1. Sommerová L, Michalová E,Hrstka R. New Approaches for Chemosensitivity Testing in Malignant Diseases. Klinicka Onkologie. 2018;31(2).
- 2. Antoni D, Burckel H, Josset E,Noël G. Three-Dimensional Cell Culture: A Breakthrough in Vivo. International Journal of Molecular Sciences. 2015;16(3):5517-27.
- 3. Amiri FZ, Pashandi Z, Lotfibakhshaiesh N, Mirzaei-Parsa MJ, Ghanbari H,Faridi‐Majidi R. Cell Attachment Effects of Collagen Nanoparticles on Crosslinked Electrospun Nanofibers. The International Journal of Artificial Organs. 2020;44(3):199-207.
- 4. Evlashin SA, Dyakonov PV, Tarkhov MA ve ark. Flexible Polycaprolactone and Polycaprolactone/Graphene Scaffolds for Tissue Engineering. Materials. 2019;12(18):2991.
- 5. Renó CO, Lima BFAS, Sousa Ed, Bertran CA,Motisuke M. Scaffolds of Calcium Phosphate Cement Containing Chitosan and Gelatin. Materials Research. 2013;16(6):1362-5.
- 6. Beran M, Berkova E, MusÍLkovÁ J, Sedlář A, Slepička P,Fajstavr D. Mineralised Polylactide and Polycaprolactone Soft Foams With Hierarchical Micro-Macro Porous Structure for Tissue Engineering. 2021.
- 7. McAllister B, Thajudeen M, Phelan SA,Sundarram SS. Study on Dual Pore Network Polylactic Acid Scaffolds for Growth of McF7 Human Breast Cancer Cells. Advanced Engineering Materials. 2022;24(9).
- 8. Sharma C, Dinda AK,Mishra NC. Fabrication and Characterization of Natural Origin Chitosan‐ Gelatin‐alginate Composite Scaffold by Foaming Method Without Using Surfactant. Journal of Applied Polymer Science. 2012;127(4):3228-41.
Details
| Primary Language | Turkish |
|---|---|
| Subjects | Biochemistry and Cell Biology (Other), Clinical Sciences (Other) |
| Journal Section | Araştırma Makalesi |
| Authors | |
| Publication Date | June 27, 2025 |
| Submission Date | October 21, 2024 |
| Acceptance Date | November 25, 2024 |
| Published in Issue | Year 2025 Volume: 12 Issue: 2 |
