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Pırlak Koyunlarında Kalpastatin (CAST) ve Miyostatin (MSTN) Genlerinde Polimorfizm

Year 2025, Volume: 13 Issue: 1, 115 - 122, 07.07.2025

Bülent Özkan , Emine Şahin Semerci

https://doi.org/10.33202/comuagri.1623981

Abstract

Bu çalışmada, Akdeniz Bölgesi'nde yaygın olarak yetiştirilen yerli Pırlak koyunlarında, Kalpastatin (CAST) ve Miyostatin (MSTN) genlerinin polimorfik yapısı incelenmiştir. Antalya ilindeki farklı işletmelerden 181 baş koyunda PCR-RFLP yöntemi uygulanarak CAST geninde MspI, MSTN geninde ise HaeIII polimorfizmi araştırılmıştır. CAST geninde MM (0.220), MN (0.696) ve NN (0.082) genotipleri tespit edilmiştir. M ve N alel frekansları sırasıyla 0.569 ve 0.431 olarak hesaplanmıştır. Buna karşılık, MSTN gen bölgesi tamamen monomorfik yapı sergileyerek yalnızca mm genotipi bulunmuştur. Ayrıca, CAST geninde Hardy-Weinberg dengesinden sapma olduğu belirlenmiştir. Elde edilen veriler, CAST genindeki polimorfik yapının genetik marker olarak kullanılabileceğini ve moleküler düzeyde yapılacak seleksiyon çalışmalarına katkı sağlayabileceğini ortaya koymuştur.

Keywords

Koyun Kalpastatin Miyostatin PCR-RFLP Polimorfizm

Ethical Statement

Akdeniz Üniversitesi Deney Hayvanları Uygulama ve Araştırma Merkezi

Supporting Institution

Akdeniz Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi

Project Number

FYL-2023-6279

Thanks

Akdeniz Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimine tez çalışmamın uygulanabilmesi için sağladıkları finansal destekten dolayı teşekkür ederim.

References

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  • Akbari, S., Dashab, G., Alipanah, M., Rokouei, M., Saghi, D., 2015. Study on the polymorphism in myostatin gene and its association with growth traits in kordi sheep of northern khorasan. Modern Genetics Journal. 10(1): 129-133.
  • Ansary, M., Tahmoorespur, M., Nassiry, MR., Taheri, A., Vafaye Valleh, M., 2011. Polymorphism in intron-ı of myostatin gene and its association with estimated breeding values of growth traits in baluchi sheep (Ovis aries). The Indian Journal of Animal Sciences. 81(8): 75-80.
  • Ata, N. 2012. Çine çapari ve karya koyunlarda calpastatin gen polimorfizminin PCR-RFLP yöntemi ile belirlenmesi. Yüksek Lisans Tezi, Adnan Menderes Üniversitesi, Aydın, Türkiye.
  • Avanus, K., 2015. Genetic Variability of CAST Gene in Native Sheep Breeds of Turkey. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 21(6): 789-794.
  • Azari, MA., Dehnavi, E., Yousefi, S., Shahmohamadi, L., 2012. Polymorphism of calpastatin, calpain and myostatin genes in native dalagh sheep in Iran. Slovak Journal Animal Sciences. 45(1): 1-6.
  • Balcıoğlu, MS., Karslı, T., Şahin Semerci, E., Ulutaş, Z., Aksoy, Y., 2014. Türkiye’de Yetiştirilen Bazı Yerli Koyun Irklarında Kalpastatin (CAST) Geni Polimorfizminin PCR-RFLP Yöntemiyle Belirlenmesi. Tarım Bilimleri Dergisi. 20(4): 427-433.
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  • Bayrak, G, 2023. Osmaniye ilinde yetiştirilen bazi koyun irklarinin CAST ve DGAT1 genotiplerinin belirlenmesi. Yüksek Lisans Tezi, Eskişehir Osmangazi Üniversitesi, Eskişehir, Türkiye.
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  • Boman, IA., Klemetsdal, G., Nafstad, O., Blichfeldt, T., Vage, DI., 2010. Impact of two myostatin (MSTN) mutations on weight gain and lamb carcass classification in Norwegian White Sheep (Ovis aries). Genetics Selection Evolution. 42(1): 1-7.
  • Bozhilova-Sakova, M., Dimitrova, I., Teneva, A., Petrov, NM., 2016. PCR-RFLP Analysis of MSTN gene in karakachan sheep breed. Bulgarian Journal of Agricultural Science. 22(1): 115-117.
  • Bozhilova-Sakova, M., Dimitrova, I., Teneva, A., Petrov, NM., 2020. Genotype frequencies in calpastatin (CAST) and callipyge (CLPG) genes in Northeast Bulgarian Merino sheep breed using PCR-RFLP method. Bulgarian Journal of Agricultural Science. 26(2): 475-479.
  • Broad, TE., Glass, BC., Greer, GJ., Robertson, TM., Bain, WE., Lord, EA., Mcewan, JC., 2000. Search for a locus near to myostatin that increases muscling in texel sheep in New Zealand. Proceedings of New Zealand Society of Animal Production. 60: 110-112.
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  • Dehnavi, E., Ahani M., Saeed, A., Mohammad H., Nassiry, R., Mohajer, M., Ahmadi, AK., Shahmohamadi, L., Yousefi, S., 2012. Polymorphism of Myostatin Gene in Intron 1 and 2 and Exon 3, and Their Associations with Yearling Weight, Using PCR-RFLP and PCR-SSCP Techniques in Zel Sheep. Biotechnology Research International. 2012(6628): 472307.
  • Djadid, ND., Nikmard, M., Zakeri, S., Gholizadeh, S., 2011. Characterization of calpastatin gene in iranian afshari sheep. İranian Journal of Biotechnology. 9(2): 145-149. Doğru, Ü., Özdemir, M., 2002. Sığırlarda Süt Protein Polimorfizmi’nin Anlam ve Önemi. Atatürk Üniversitesi, Ziraat Fakültesi Dergisi, 33(4): 457-464.
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  • Gharahveysi, S., Abbasi, HA., Irani, M., Abdullahpour, R., Mirhabibi, S., 2012. Polymorphism investigation of calpastatin gene in zel sheep population of iran by PCR-RFLP method. African Journal of Biotechnology. 11(13): 3211-3214.
  • Hickford, JG., Forrest, RH., Zhou, H., Fang, Q., Han, J., Frampton, CM., Horrell, AL., 2010. Polymorphisms in the ovine myostatin gene (MSTN) and their association with growth and carcass traits in New Zealand romney sheep. Animal Genetics. 41(1): 64-72.
  • Huff-Lonergan, EJ., Mitsuhashi, T., Beekman, DD., Parrish, J., Olson, DG., Robson, RM., 1996. Proteolysis of specific muscle structural proteins by mu-calpain is similar to degradation in postmortem bovine muscle. Journal Animal Science. 74(5): 993-1008.
  • Jamshidi, S., Karani, S., Goudarzi, M., 2014. Study polyporphism myostatin gene in mehraban’s sheep using PCR- RFLP method. Science International Lahore. 26(3): 1129-1135.
  • Jawasreh, KI., Jadallah, R., Al-Amareen, AH., Abdullah, AY., Al-Qaisi, A., Alrawashdeh, IM., Al-Zghoul, MBF., Ahamed, MKA., Obeidat, B., 2017. Association between MspI calpastatin gene polymorphisms, growth performance, and meat characteristics of awassi sheep. Indian Journal of Animal Sciences. 87(5): 635- 639.
  • Johnson, PL., Mcewan, JC., Dodds, KG., Purchas, RW., Blair, HT., 2005. A directed search in the region of GDF8 for quantitative trait loci affecting carcass traits in texel sheep. Journal of Animal Science. 83(9): 1988- 2000.
  • Kaplan, S., Atalay S., 2017. Determination of Calpastatin Gene Polymorphism in Kıvırcık Crossbred Ewesby PCR-RFLP Method. Selcuk Journal of Acriculture and Food Sciences. 31(3): 147-150.
  • Kar, B., Öz, B., Yazdıç, FD., Altuğ, K., 2024. Effects of mutation in myostatin exon 3 on growth and carcass characteristics of Savak Akkaraman sheep. Small Ruminant Research. 241(2024): 107386.
  • Kırıkçı, K., 2022. Polymorphism of the Calpastatin (CAST) and Growth Differentiation Factor 9 (GDF9) genes in Akkaraman Sheep Breed. Journal Animal Produse. 63(1): 21-26.
  • Khederzadeh, S., 2011. Polymorphism of calpastatin gene in crossbreed dalagh sheep using PCR-RFLP. African Journal Biotechnology. 10(53): 10839-10841.
  • Lonergan, SM., Ernst, CW., Bishop, MD., Calkins, CR., Koohmaraie, M., 1995. Relationship of restriction fragment length polymorphisms (RFLP) at the bovine calpastatin locus to calpastatin activity and meat tenderness. Journal Animal Science. 73(12): 08-12.
  • Marcq, F., Larzul, C., Marot, V., Bouix, J., Eychenne, F., Laville, E., Georges, M., Bibe, B., Leroy, PL., Elsen, JM., 2002. Preliminary results of a whole genome scan targeting QTL for carcass traits in a texel romanov intercross. Proceedings of the 7th World Congress on Applied Livestock Production, 19-23 August, Montpellier, pp. 02-14.
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Pırlak Koyunlarında Kalpastatin (CAST) ve Miyostatin (MSTN) Gen Polimorfizmleri

Year 2025, Volume: 13 Issue: 1, 115 - 122, 07.07.2025

Bülent Özkan , Emine Şahin Semerci

https://doi.org/10.33202/comuagri.1623981

Abstract

Bu çalışmada, Akdeniz Bölgesi'nde yaygın olarak yetiştirilen yerli Pırlak koyunlarında, Kalpastatin (CAST) ve Miyostatin (MSTN) genlerinin polimorfik yapısı incelemiştir. Antalya ilindeki farklı işletmelerden 181 baş koyunda PCR-RFLP yöntemi uygulanarak CAST geninde MspI, MSTN geninde ise HaeIII polimorfizmi araştırılmıştır. CAST geninde MM (0.220), MN (0.696) ve NN (0.082) genotipleri tespit edilmiştir. M ve N alel frekansları sırasıyla 0.569 ve 0.431 olarak hesaplanmıştır. Buna karşılık, MSTN gen bölgesi tamamen monomorfik yapı sergileyerek yalnızca mm genotipi bulunmuştur. Ayrıca, CAST geninde Hardy-Weinberg dengesinden sapma olduğu belirlenmiştir. Elde edilen veriler, CAST genindeki polimorfik yapının genetik marker olarak kullanılabileceğini ve moleküler düzeyde yapılacak seleksiyon çalışmalarına katkı sağlayabileceğini ortaya koymuştur.

Keywords

Koyun Kalpastatin Miyostatin PCR-RFLP Polimorfizm

Project Number

FYL-2023-6279

References

  • Aali, M., Moradi-Shahrbabak, H., Moradi-Shahrbabak, M., Sadeghi, M., Yousefi, AR., 2017. Association of the calpastatin genotypes, haplotypes, and SNPs with meat quality and fatty acid composition in two Iranian fat-and thin-tailed sheep breeds. Small Ruminant Reserarch. 149(2): 40-51.
  • Akbari, S., Dashab, G., Alipanah, M., Rokouei, M., Saghi, D., 2015. Study on the polymorphism in myostatin gene and its association with growth traits in kordi sheep of northern khorasan. Modern Genetics Journal. 10(1): 129-133.
  • Ansary, M., Tahmoorespur, M., Nassiry, MR., Taheri, A., Vafaye Valleh, M., 2011. Polymorphism in intron-ı of myostatin gene and its association with estimated breeding values of growth traits in baluchi sheep (Ovis aries). The Indian Journal of Animal Sciences. 81(8): 75-80.
  • Ata, N. 2012. Çine çapari ve karya koyunlarda calpastatin gen polimorfizminin PCR-RFLP yöntemi ile belirlenmesi. Yüksek Lisans Tezi, Adnan Menderes Üniversitesi, Aydın, Türkiye.
  • Avanus, K., 2015. Genetic Variability of CAST Gene in Native Sheep Breeds of Turkey. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 21(6): 789-794.
  • Azari, MA., Dehnavi, E., Yousefi, S., Shahmohamadi, L., 2012. Polymorphism of calpastatin, calpain and myostatin genes in native dalagh sheep in Iran. Slovak Journal Animal Sciences. 45(1): 1-6.
  • Balcıoğlu, MS., Karslı, T., Şahin Semerci, E., Ulutaş, Z., Aksoy, Y., 2014. Türkiye’de Yetiştirilen Bazı Yerli Koyun Irklarında Kalpastatin (CAST) Geni Polimorfizminin PCR-RFLP Yöntemiyle Belirlenmesi. Tarım Bilimleri Dergisi. 20(4): 427-433.
  • Barzehkar, R., Salehi, A., Mahjoubi, F., 2009. Polymorphisms of the ovine leptin gene and its association with growth and carcass traits in three iranian sheep breeds. Iranian Hournal of Biotechnology. 7(4): 241-246.
  • Bayrak, G, 2023. Osmaniye ilinde yetiştirilen bazi koyun irklarinin CAST ve DGAT1 genotiplerinin belirlenmesi. Yüksek Lisans Tezi, Eskişehir Osmangazi Üniversitesi, Eskişehir, Türkiye.
  • Bayram, M., Akyüz, B., Arslan, K., Özdemir, F., Aksel, EG., Çınar, MU., 2019. DGAT1, CAST and IGF-I gene polymorphisms in Akkaraman lambs and their effects on live weights up to weaning age. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 25(1): 9-15.
  • Boehm, ML., Kendall, TL., Thompson, VF., Goll, DE., 1998. Changes in the calpains and calpastatin during postmortem storage of bovine muscle. Journal Animal Science. 76(9): 2415-2434.
  • Boman, IA., Klemetsdal, G., Nafstad, O., Blichfeldt, T., Vage, DI., 2010. Impact of two myostatin (MSTN) mutations on weight gain and lamb carcass classification in Norwegian White Sheep (Ovis aries). Genetics Selection Evolution. 42(1): 1-7.
  • Bozhilova-Sakova, M., Dimitrova, I., Teneva, A., Petrov, NM., 2016. PCR-RFLP Analysis of MSTN gene in karakachan sheep breed. Bulgarian Journal of Agricultural Science. 22(1): 115-117.
  • Bozhilova-Sakova, M., Dimitrova, I., Teneva, A., Petrov, NM., 2020. Genotype frequencies in calpastatin (CAST) and callipyge (CLPG) genes in Northeast Bulgarian Merino sheep breed using PCR-RFLP method. Bulgarian Journal of Agricultural Science. 26(2): 475-479.
  • Broad, TE., Glass, BC., Greer, GJ., Robertson, TM., Bain, WE., Lord, EA., Mcewan, JC., 2000. Search for a locus near to myostatin that increases muscling in texel sheep in New Zealand. Proceedings of New Zealand Society of Animal Production. 60: 110-112.
  • Cockett, NE., Jackson, SP., Shay, TL., Farnir, F., Berghmans, S., Snowder, GD., Nielsen, DM., Georges, M., 1996. Polar overdominance at the ovine callipyge locus. Science. 273(5272): 236-238.
  • Dehnavi, E., Ahani M., Saeed, A., Mohammad H., Nassiry, R., Mohajer, M., Ahmadi, AK., Shahmohamadi, L., Yousefi, S., 2012. Polymorphism of Myostatin Gene in Intron 1 and 2 and Exon 3, and Their Associations with Yearling Weight, Using PCR-RFLP and PCR-SSCP Techniques in Zel Sheep. Biotechnology Research International. 2012(6628): 472307.
  • Djadid, ND., Nikmard, M., Zakeri, S., Gholizadeh, S., 2011. Characterization of calpastatin gene in iranian afshari sheep. İranian Journal of Biotechnology. 9(2): 145-149. Doğru, Ü., Özdemir, M., 2002. Sığırlarda Süt Protein Polimorfizmi’nin Anlam ve Önemi. Atatürk Üniversitesi, Ziraat Fakültesi Dergisi, 33(4): 457-464.
  • Dokgöz, M., 2023. Osmaniye İlinde Halk Elinde Yetiştirilen İvesi ve Akkaraman Koyunlarında Kalpastatin Gen Polimorfizminin PCR-RFLP Yöntemi İle Belirlenmesi. Yüksek Lisans Tezi, Çukurova Üniversitesi, Adana, Türkiye.
  • Durmaz, M., 2021. Şanlıurfa ilinde yetiştirilen zom koyunlarinda kalpastatin (CAST) gen polimorfizmi. Yüksek Lisans Tezi, Harran Üniversitesi, Şanlıurfa, Türkiye.
  • Ertuğrul, M., Akman, N., Dellal, G., Goncagül, T., 2000. Hayvan gen kaynaklarinin korunmasi ve türkiye hayvan gen kaynaklari. V. Türkiye Ziraat Mühendisliği Teknik Kongresi, 17-21 Ocak, Ankara, s. 285-300.
  • Farhadian, M., Hashemi, A., Mardani, K., Darvishzadeh, R., Jafari, S., 2012. Polymorphisms in the ovine myostatin gene are associated with birth weight but not with weight gain in iranian makoei sheep. Genetics Molecular Research. 11(4): 3568-3575.
  • Gharahveysi, S., Abbasi, HA., Irani, M., Abdullahpour, R., Mirhabibi, S., 2012. Polymorphism investigation of calpastatin gene in zel sheep population of iran by PCR-RFLP method. African Journal of Biotechnology. 11(13): 3211-3214.
  • Hickford, JG., Forrest, RH., Zhou, H., Fang, Q., Han, J., Frampton, CM., Horrell, AL., 2010. Polymorphisms in the ovine myostatin gene (MSTN) and their association with growth and carcass traits in New Zealand romney sheep. Animal Genetics. 41(1): 64-72.
  • Huff-Lonergan, EJ., Mitsuhashi, T., Beekman, DD., Parrish, J., Olson, DG., Robson, RM., 1996. Proteolysis of specific muscle structural proteins by mu-calpain is similar to degradation in postmortem bovine muscle. Journal Animal Science. 74(5): 993-1008.
  • Jamshidi, S., Karani, S., Goudarzi, M., 2014. Study polyporphism myostatin gene in mehraban’s sheep using PCR- RFLP method. Science International Lahore. 26(3): 1129-1135.
  • Jawasreh, KI., Jadallah, R., Al-Amareen, AH., Abdullah, AY., Al-Qaisi, A., Alrawashdeh, IM., Al-Zghoul, MBF., Ahamed, MKA., Obeidat, B., 2017. Association between MspI calpastatin gene polymorphisms, growth performance, and meat characteristics of awassi sheep. Indian Journal of Animal Sciences. 87(5): 635- 639.
  • Johnson, PL., Mcewan, JC., Dodds, KG., Purchas, RW., Blair, HT., 2005. A directed search in the region of GDF8 for quantitative trait loci affecting carcass traits in texel sheep. Journal of Animal Science. 83(9): 1988- 2000.
  • Kaplan, S., Atalay S., 2017. Determination of Calpastatin Gene Polymorphism in Kıvırcık Crossbred Ewesby PCR-RFLP Method. Selcuk Journal of Acriculture and Food Sciences. 31(3): 147-150.
  • Kar, B., Öz, B., Yazdıç, FD., Altuğ, K., 2024. Effects of mutation in myostatin exon 3 on growth and carcass characteristics of Savak Akkaraman sheep. Small Ruminant Research. 241(2024): 107386.
  • Kırıkçı, K., 2022. Polymorphism of the Calpastatin (CAST) and Growth Differentiation Factor 9 (GDF9) genes in Akkaraman Sheep Breed. Journal Animal Produse. 63(1): 21-26.
  • Khederzadeh, S., 2011. Polymorphism of calpastatin gene in crossbreed dalagh sheep using PCR-RFLP. African Journal Biotechnology. 10(53): 10839-10841.
  • Lonergan, SM., Ernst, CW., Bishop, MD., Calkins, CR., Koohmaraie, M., 1995. Relationship of restriction fragment length polymorphisms (RFLP) at the bovine calpastatin locus to calpastatin activity and meat tenderness. Journal Animal Science. 73(12): 08-12.
  • Marcq, F., Larzul, C., Marot, V., Bouix, J., Eychenne, F., Laville, E., Georges, M., Bibe, B., Leroy, PL., Elsen, JM., 2002. Preliminary results of a whole genome scan targeting QTL for carcass traits in a texel romanov intercross. Proceedings of the 7th World Congress on Applied Livestock Production, 19-23 August, Montpellier, pp. 02-14.
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There are 54 citations in total.

Details

Primary Language Turkish
Subjects Zootechny (Other)
Journal Section Articles
Authors

Bülent Özkan 0000-0002-7139-839X

Emine Şahin Semerci 0000-0001-8471-9300

Project Number FYL-2023-6279
Publication Date July 7, 2025
Submission Date January 20, 2025
Acceptance Date June 29, 2025
Published in Issue Year 2025 Volume: 13 Issue: 1

Cite

APA Özkan, B., & Şahin Semerci, E. (2025). Pırlak Koyunlarında Kalpastatin (CAST) ve Miyostatin (MSTN) Genlerinde Polimorfizm. ÇOMÜ Ziraat Fakültesi Dergisi, 13(1), 115-122. https://doi.org/10.33202/comuagri.1623981