Research Article
Immunohistochemical Expression of Beclin1 and Lc3 in the Ovary and Oviduct during Early Pregnancy in Rats
Abstract
Proteins associated with autophagy are called (Autophagy related proteins) Atg proteins. Atg6 (Beclin 1) complex regulates autophagy at the molecular level and lyses cytosolic proteins, organelles and cytoplasmic components in the autophagosome during autophagy. LC3 is an autophagosomal marker. Monitoring autophagy-related processes is very important in understanding pregnancy metabolism. In this study, Beclin 1 and LC3 were immunohistochemically demonstrated in the ovary and oviduct on day 5 in the first pregnancy period. Accordingly, on the 5th day of pregnancy, it was determined that autophagy continued to increase in pregnancy with the immunohistochemical (+) of Beclin 1, LC3 in interstitial cells, germinative epithelium, follicle granulosa cells and theca interna/externa in the ovary and epithelial cells in the oviduct.
Keywords
References
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- Zhou JZ, Way SS, Chen K (2018). Immunology of the uterine and vaginal mucosae. Trends Immunol, 39(4):302-314.
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- Zhao F, Zhao W, Ren S et al. (2014). Roles of SIRT1 in granulosa cell apoptosis during the process of follicular atresia in porcine ovary. Anim Reprod Sci, 151(1-2):34-41.
- Klionsky DJ (2012). The autophagy community. Autophagy, 8(7):1003-1003.
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- Stocco C, Telleria C, Gibori G (2007). The molecular control of corpus luteum formation, function, and regression. Endocr Rev, 28(1):117-149.
- Tang X, Liu B, Wang X et al. (2018). Epidermal growth factor, through alleviating oxidative stress, protect IPEC-J2 cells from lipopolysaccharides-induced apoptosis. Int J Mol Sci, 19(3):848.
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- Choi J, Jo M, Lee E et al. (2011). The role of autophagy in corpus luteum regression in the rat. Biol Reprod, 85(3):465-472.
Ratlarda Gebeliğin Erken Döneminde Ovaryum ve Oviduktta Beclin1 ile LC3’ün İmmunohistokimyasal İfadesi
Abstract
Otofaji ile bağlantılı proteinler (Autophagy Related Proteins), Atg proteinleri olarak isimlendirilir. Otofajinin moleküler düzeyde düzenlenmesinde Atg6 (Beclin 1) kompleksi ve otofaji sırasında otofagozomda sitozolik proteinleri, organelleri, sitoplazmik bileşenleri lize eden LC3 ise otofagozomal işaretleyicilerdendir. Otofaji ile ilgili süreçlerin izlenmesi gebelik metabolizmasının anlaşılmasında oldukça önemlidir. Yapılan çalışmada gebeliğin birinci periyodu içinde 5. günde ovaryum ve oviduktta Beclin 1, LC3 immunohistokimyasal olarak gösterilmiştir. Buna göre gebeliğin 5. gününde ovaryumda intertisyel hücreler, germinatif epitelde, folikül granüloza hücrelerinde ve teka interna/eksternada, oviduktta ise epitel hücrelerinde, Beclin 1, LC3’ün immunohistokimyasal olarak (+) liği ile otofajinin gebelikte artarak devam ettiği belirlenmiştir.
Keywords
References
- Ozpolat B, Benbrook DM (2015). Targeting autophagy in cancer management–strategies and developments. Cancer Manag Res, 291-299.
- Kadowaki M, Karim MR, Carpi A et al. (2006). Nutrient control of macroautophagy in mammalian cells. Mol Aspects Med, 27(5-6):426-443.
- Al Rawi S, Louvet-Vallee S, Djeddi A et al. (2011). Postfertilization autophagy of sperm organelles prevents paternal mitochondrial DNA transmission. Science, 334 (6059):1144-1147.
- Sato M, Sato K (2011). Degradation of paternal mitochondria by fertilization-triggered autophagy in C. elegans embryos. Science, 334:1141-1144.
- Song WH, Yi YJ, Sutovsky M et al. (2016). Autophagy and ubiquitin-proteasome system contribute to sperm mitophagy after mammalian fertilization. Proc Natl Acad Sci, 113(36):e526-570.
- Stitzel ML, Seydoux G (2007). Regulation of the oocyte-to-zygote transition. Science, 316(5823):407-408.
- Di Bartolomeo S, Nazio F, Cecconi F (2010). The role of autophagy during development in higher eukaryotes. Traffic, 11(10):1280-1289.
- Xie Z, Klionsky DJ (2007). Autophagosome formation: core machinery and adaptations. Nat Cell Biol, 9(10):1102.
- Arslan DÖ, Korkmaz G, Gözüaçık D (2011). Otofaji: Bir hücresel stres yanıtı ve ölüm mekanizması. AÜHSJ, 4:184-194.
- Mooren FC, Krüger K (2015). Exercise, autophagy, and apoptosis. Prog Mol Biol Transl Sci, 135:407-422.
- Chen S, Rehman SK, Zhang W et al. (2010). Autophagy is a therapeutic target in anticancer drug resistance. Biochim Biophys Acta (BBA) Bioenerg, 1806:220-229.
- Aita VM, Liang XH, Murty VV et al. (1999). Cloning and genomic organization of beclin 1, a candidate tumor suppressor gene on chromosome 17q21. Genomics, 59(1):59-65.
- Gawriluk TR, Rucker EB (2015). BECN1, corpus luteum function, and preterm labor. Autophagy, 11(1):183-184.
- Uslu D, Uslu S (2024). Immunohistochemical investigation of autophagy in the uterus during the first trimester of pregnancy in rats. Van Vet J, 35(1):59-63.
- Mizushima N, Yoshimori T, Ohsumi Y (2011). The role of Atg proteins in autophagosome formation. Annu Rev Cell Dev Biol, 27:107-132.
- Zhou JZ, Way SS, Chen K (2018). Immunology of the uterine and vaginal mucosae. Trends Immunol, 39(4):302-314.
- Choi JY, Jo MW, Lee EY et al. (2010). The role of autophagy in follicular development and atresia in rat granulosa cells. Fertil Steril, 93:2532-2537.
- Zhang C, Hu J, Wang W et al. (2020). HMGB1-induced aberrant autophagy contributes to insulin resistance in granulosa cells in PCOS. FASEB J, 34(7):9563-9574.
- Cao B, Camden AJ, Parnell LA et al. (2017). Autophagy regulation of physiological and pathological processes in the female reproductive tract. Am J Reprod Immunol, 77(5):e12650.
- Kumariya S, Ubba V, Jha RK et al. (2021). Autophagy in ovary and polycystic ovary syndrome: role, dispute and future perspective. Autophagy, 17(10):2706-2733.
- Zhao F, Zhao W, Ren S et al. (2014). Roles of SIRT1 in granulosa cell apoptosis during the process of follicular atresia in porcine ovary. Anim Reprod Sci, 151(1-2):34-41.
- Klionsky DJ (2012). The autophagy community. Autophagy, 8(7):1003-1003.
- Wu L, Zhang Z, Pan X et al. (2015). Expression and contribution of the HIF-1α/VEGF signaling pathway to luteal development and function in pregnant rats. Mol Med Rep, 12(5):7153-7159.
- Carambula SF, Matikainen T, Lynch MP et al. (2002). Caspase-3 is a pivotal mediator of apoptosis during regression of the ovarian corpus luteum. Endocrinology, 143(4):1495-1501.
- Stocco C, Telleria C, Gibori G (2007). The molecular control of corpus luteum formation, function, and regression. Endocr Rev, 28(1):117-149.
- Tang X, Liu B, Wang X et al. (2018). Epidermal growth factor, through alleviating oxidative stress, protect IPEC-J2 cells from lipopolysaccharides-induced apoptosis. Int J Mol Sci, 19(3):848.
- Hung TH, Chen SF, Lo LM et al. (2012). Increased autophagy in placentas of intrauterine growth-restricted pregnancies. PLoS One, 7(7):e40957.
- Gong JS, Kim GJ (2014). The role of autophagy in the placenta as a regulator of cell death. CERM, 41(3):97.
- Bildirici I, Longtine MS, Chen B et al. (2012). Survival by self-destruction: a role for autophagy in the placenta?. Placenta, 33(8):591-598.
- Chifenti B, Locci MT, Lazzeri G et al. (2013). Autophagy-related protein LC3 and Beclin-1 in the first trimester of pregnancy. CERM, 40(1):33.
- Choi J, Jo M, Lee E et al. (2011). The role of autophagy in corpus luteum regression in the rat. Biol Reprod, 85(3):465-472.
There are 31 citations in total.
Details
| Primary Language | English |
|---|---|
| Subjects | Veterinary Histology and Embryology |
| Journal Section | Research |
| Authors | |
| Publication Date | June 30, 2025 |
| Submission Date | February 13, 2025 |
| Acceptance Date | April 11, 2025 |
| Published in Issue | Year 2025 Volume: 18 Issue: 1 |
