Research Article
Year 2025,
Volume: 66 Issue: 1, 73 - 81, 01.07.2025
Abstract
Amaç: Bu araştırmada; sığırlarda boynuzsuzluğa neden olan Celtic boynuzsuzluk (PolC) ve Fresian boynuzsuzluk (PolF) varyantlarının, Eskişehir ilinde yetiştirilen Siyah Alaca sığır popülasyonundaki varlıkları PCR ve DNA dizi analizi yöntemlerii kullanılarak incelenmiştir.
Materyal ve Metot: Araştırmanın hayvan materyali olarak Eskişehir bölgesindeki bazı işletmelerde yetiştirilen toplam 100 baş sığırdan alınan kan örneklerinden izole edilen DNAlar kullanılmıştır. Araştırmada, PolC ve PolF varyantlarının tespiti için PCR reaksiyonu ile çoğaltılmış, PCR ürünleri agaroz jelde kontrol edilerek DNA dizi analizi yapılmıştır.
Bulgular: Eskişehir’de yetiştirilen 100 baş Siyah Alaca sığırla PolF varyantı tespit edilemezken PolC alleline sahip bir heterozigot sığır tespit edilmiştir.
Sonuç: Modern sığır endüstrisinde boynuzsuz sığırların daha fazla tercih edilirken moleküler yöntemler pol genotipli sığırların homozigot-heterozigot durumunun belirlenmesinde yol göstererek sürü yönetimi ve planlamasına katkıda bulunacaktır.
Ethical Statement
Örnek toplama işlemine ilişkin prosedürler Eskişehir Osmangazi Üniversitesi Tıbbi ve Cerrahi Deneysel Araştırma Merkezi Komisyonu (TİKAM) 2011/242 sayılı Kararı ile onaylanmıştır.
Supporting Institution
Eskişehir Osmangazi Üniversitesi BAP
Project Number
FLO-2024-3010
References
- Aldersey JE, Sonstegard TS, Williams JL, and Bottema CDK. 2020. Understanding the effects of the bovine POLLED variants. Anim Genet, 51, 166–176, https://doi.org/10.1111/age.12915 .
- Allais-Bonnet A, Grohs C, Medugorac I, Krebs S, Djari A, Graf A, Fritz S, Seichter D, Baur A, Russ I, Bouet S, Rothammer S, Wahlberg P, Esouerré D, Hoze C, Boussaha M, Weiss B, Thépot D, Fouilloux M, Rossignol M, van Marle-Köster E, Hreiðarsdóttir GE, Barbey S, Dozias D, Cobo E, Reversé P, Catros O, Marchand J, Soulas P, Roy P, Marquant-Leguienne B, Le Bourhis D, Clément L, Salas-Cortes L, Venot E, Pannetier M, Phocas F, Klopp C, Rocha D, Fouchet M, Journaux L, Bernard-Capel C, Ponsart C, Eggen A, Blum H, Gallard Y, Boichard D, Pailhoux E, Capitan A. 2013. Novel insights into the bovine polled phenotype and horn ontogenesis in Bovidae. PLoS One, 8, e63512. https://doi.org/10.1371/journal.pone.0063512 .
- Barendse W, Armitage SM, Kossarek LM, Shalom A, Kirkpatrick BW, Ryan AM, Clayton D, Li L, Neibergs HL, Zhang N, Grosse WM, Weiss J, Creighton P, McCarthy F, Ron M, Teale AJ, Fries R, McGraw RA, Moore SS, Georges M, Soller M, Womack JE, Hetzel DJS. 1994. A genetic linkage map of the bovine genome. Nat Genet, 6:227–235.
- Bovine Genome Sequencing and Analysis Consortium; 2009. The genome sequence of taurine cattle: a window to ruminant biology and evolution. Science. 324(5926):522-8. doi: https://doi.org/10.1126/science.1169588
- Brenneman RA, Davis SK, Sanders JO, Burns BM, Wheeler TC, Turner JW, Taylor JF. 1996. The polled locus maps to BTA1 in a Bos indicus× Bos taurus cross. J Hered, 87(2), 156-161
- Brockmann GA, Martin J, Teuscher F, and Schwerin M. 2000. Marker controlled inheritance of the polled locus in Simmental cattle, Arch. Anim. Breed., 43, 207–212, https://doi.org/10.5194/aab-43-207-2000
- Caray D, des Roches ADB, Frouja S, Andanson S, and Veissier I. 2015. Hot-iron disbudding: stress responses and behavior of 1-and 4-week-old calves receiving anti-inflammatory analgesia without or with sedation using xylazine. Lives Sci, 179, pp.22-28.
- Carlson DF, Lancto CA, Zang B, Kim E-S, Walton M, Oldeschulte D, Seabury C, Sonstegard TS, Fahrenkrug SC. 2016. Production of hornless dairy cattle from genome-edited cell lines. Nat Biotechnol, 34;479–481.
- Chamberlain A. 2017. Identification of a Horned and Polled Bos taurus using a Gene Test. Degree of Bachelor of Agricultural Science, Lincoln University
- Chen SY, Liu LH, Fu MZ, Zhang GW, Yi J, Lai SJ, Wang W. 2017. Simultaneous introgression of three POLLED mutations into a synthetic breed of Chinese cattle. PLOS ONE 12, e0186862.
- CodonCode Corporation. 2024. www.codoncode.com . Erişim Tarihi: 22/12/2024
- Drögemüller C, Wöhlke A, Mömke S, Distl O. 2005. Fine mapping of the polled locus to a 1-Mb region on bovine chromosome 1q12. Mamm Genome. 16, 613–620. https://doi.org/10.1007/s00335-005-0016-0 .
- Georges M, Drinkwater R, King T, Mishra A, Moore SS, Nielsen D, Sargeant LS, Sorensen A, Steele MR, Zhao X, Womack JE, Hetzel J. 1993. Microsatellite mapping of a gene affecting horn development in Bos taurus. Nat Genet, 4(2), 206-210. https://doi.org/10.1038/ng0693-206
- Götz K, Luntz B, Robeis J, Edel C, Emmerling R, Buitkamp J, Anzenberger H, Duda J. 2015. Polled Fleckvieh (Simmental) cattle - current state of the breeding program. Lives Sci, 179, 80–85.
- Grobler R, van Marle-Köster E, and Visser C. 2021. Challenges in selection and breeding of polled and scur phenotypes in beef cattle. Lives Sci, 247, p.104479.
- Kling-Eveillard F, Knierim U, Irrgang N, Gottardo F, Ricci R, Dockès AC. 2015. Attitudes of farmers towards cattle dehorning. Lives Sci, 179, 12-21.
- Kumar S, Stecher G, Suleski M, Sanderford M, Sharma S, and Tamura K. 2024. MEGA12: Molecular Evolutionary Genetics Analysis version 12 for adaptive and green computing. Molecular Biology and Evolution 41:1-9
- Mariasegaram M, Harrison BE, Bolton JA, Tier B, Henshall JM, Barendse W, and Prayaga KC. 2012. Fine‐mapping the POLL locus in B rahman cattle yields the diagnostic marker CSAFG29. Anim Genet, 43(6); 683-688.
- Medugorac I, Graf A, Grohs C, Rothammer S, Zagdsuren Y, Gladyr E, Zinovieva N, Barbieri J, Seichter D, Russ I, and Eggen A. 2017. Whole-genome analysis of introgressive hybridization and characterization of the bovine legacy of Mongolian yaks. Nat Genet, 49(3); 470-475.
- Medugorac I, Seichter D, Graf A, Russ I, Blum H, Göpel KH, Rothammer S, Förster M, and Krebs S. 2012. Bovine polledness–an autosomal dominant trait with allelic heterogeneity. PloS one, 7(6), p.e39477.
- Randhawa IA, Burns BM, McGowan MR, Porto-Neto LR, Hayes BJ, Ferretti R, Schutt KM, and Lyons RE. 2020. Optimized genetic testing for polledness in multiple breeds of cattle. G3-Genes Genom Genet, 10(2);539-544. https://doi.org/10.1534/g3.119.400866
- Rothammer S, Capitan A, Mullaart E, Seichter D, Russ I, Medugorac I. 2014. The 80-kb DNA duplication on BTA1 is the only remaining candidate mutation for the polled phenotype of Friesian origin. Genet Sel, 46:44. doi:10.1186/1297-9686-46-44
- OMIA, 2024, https://www.omia.org/OMIA001965/9913/ , Erişim Tarihi: 22/12/2024
- Schmutz SM, Marquess FLS, Berryere TG, Moker JS. 1995. DNA marker-assisted selection of the polled condition in Charolais cattle. Mamm Genome, 6(10), 710713.
- Schuster F, Aldag P, Frenzel A, Hadeler K-G, Lucas-Hahn A, Niemann H, Petersen B. 2020. CRISPR/Cas12a mediated knock-in of the Polled Celtic variant to produce a polled genotype in dairy cattle. Sci. Rep, 10(1):13570.
- Seichter D, Russ I, Rothammer S, Eder J, Förster M, & Medugorac I. 2012. SNP‐based association mapping of the polled gene in divergent cattle breeds. Anim Genet, 43(5); 595-598.
- Spurlock DM, Stock ML, Coetzee JF. 2014. The impact of 3 strategies for incorporating polled genetics into a dairy cattle breeding program on the overall herd genetic merit. Journal of Dairy Science. 1;97(8):5265-74.
- UCSC, 2025. UCSC Genome Browser Genbank. https://genome.ucsc.edu/cgi-bin/hgTracks?db=bosTau8&lastVirtModeType=default&lastVirtModeExtraState=&virtModeType=default&virtMode=0&nonVirtPosition=&position=chr1%3A1909358%2D1909509&hgsid=2319655858_gACDQZ7bXRen0DY1ySRj4OREVoc7 . Erişim tarihi: 03/01/2025.
- Utsunomiya YT, Torrecilha RBÇ, Milanesi M, Paulan SdC, Utsunomiya ATH, Garcia JF. 2019. Hornless Nellore cattle (Bos indicus) carrying a novel 110 kbç duplication variant of the polled locus. Anim Genet, 50; 187-8.
- Veterinary Genetics Laboratory. 2025, https://vgl.ucdavis.edu/test/polled-vs-horned . Erişim tarihi:03/01/2025
- Wiedemar N, Tetens J, Jagannathan V, Menoud A, Neuenschwander S, Bruggmann R, et al. (2014) Independent Polled Mutations Leading to Complex Gene Expression Differences in Cattle. PLoS ONE 9(3): e93435. https://doi.org/10.1371/journal.pone.0093435
- Zoetis. 2025, https://www2.zoetis.co.nz/products-solutions/genetics/beef/hornpoll . Erişim tarihi: 03/01/2025.
Year 2025,
Volume: 66 Issue: 1, 73 - 81, 01.07.2025
Abstract
Objective: In this study, the presence of Celtic polled (PolC) and Fresian Pollen (PolF) variants that cause hornlessness in cattle was investigated in the Holstein cattle population raised in Eskişehir province using PCR and DNA sequence analysis methods.
Material and Methods: DNA obtained from blood samples taken from a total of 100 head cattle from some farms in the Eskişehir region were used as animal material for the study. In the study, PolC and PolF variants were detected by PCR and DNA sequence, PCR products were checked on agarose gel and DNA sequence analysis was performed.
Results: While the PolF variant could not be detected in 100 Holstein cattle raised in Eskişehir, a heterozygous cattle with the PolC allele was detected.
Conclusion: While hornless cattle are preferred more in the modern cattle industry, molecular methods will contribute to herd management and planning by guiding the determination of homozygous-heterozygous status of pol genotype cattle.
Keywords
Project Number
FLO-2024-3010
References
- Aldersey JE, Sonstegard TS, Williams JL, and Bottema CDK. 2020. Understanding the effects of the bovine POLLED variants. Anim Genet, 51, 166–176, https://doi.org/10.1111/age.12915 .
- Allais-Bonnet A, Grohs C, Medugorac I, Krebs S, Djari A, Graf A, Fritz S, Seichter D, Baur A, Russ I, Bouet S, Rothammer S, Wahlberg P, Esouerré D, Hoze C, Boussaha M, Weiss B, Thépot D, Fouilloux M, Rossignol M, van Marle-Köster E, Hreiðarsdóttir GE, Barbey S, Dozias D, Cobo E, Reversé P, Catros O, Marchand J, Soulas P, Roy P, Marquant-Leguienne B, Le Bourhis D, Clément L, Salas-Cortes L, Venot E, Pannetier M, Phocas F, Klopp C, Rocha D, Fouchet M, Journaux L, Bernard-Capel C, Ponsart C, Eggen A, Blum H, Gallard Y, Boichard D, Pailhoux E, Capitan A. 2013. Novel insights into the bovine polled phenotype and horn ontogenesis in Bovidae. PLoS One, 8, e63512. https://doi.org/10.1371/journal.pone.0063512 .
- Barendse W, Armitage SM, Kossarek LM, Shalom A, Kirkpatrick BW, Ryan AM, Clayton D, Li L, Neibergs HL, Zhang N, Grosse WM, Weiss J, Creighton P, McCarthy F, Ron M, Teale AJ, Fries R, McGraw RA, Moore SS, Georges M, Soller M, Womack JE, Hetzel DJS. 1994. A genetic linkage map of the bovine genome. Nat Genet, 6:227–235.
- Bovine Genome Sequencing and Analysis Consortium; 2009. The genome sequence of taurine cattle: a window to ruminant biology and evolution. Science. 324(5926):522-8. doi: https://doi.org/10.1126/science.1169588
- Brenneman RA, Davis SK, Sanders JO, Burns BM, Wheeler TC, Turner JW, Taylor JF. 1996. The polled locus maps to BTA1 in a Bos indicus× Bos taurus cross. J Hered, 87(2), 156-161
- Brockmann GA, Martin J, Teuscher F, and Schwerin M. 2000. Marker controlled inheritance of the polled locus in Simmental cattle, Arch. Anim. Breed., 43, 207–212, https://doi.org/10.5194/aab-43-207-2000
- Caray D, des Roches ADB, Frouja S, Andanson S, and Veissier I. 2015. Hot-iron disbudding: stress responses and behavior of 1-and 4-week-old calves receiving anti-inflammatory analgesia without or with sedation using xylazine. Lives Sci, 179, pp.22-28.
- Carlson DF, Lancto CA, Zang B, Kim E-S, Walton M, Oldeschulte D, Seabury C, Sonstegard TS, Fahrenkrug SC. 2016. Production of hornless dairy cattle from genome-edited cell lines. Nat Biotechnol, 34;479–481.
- Chamberlain A. 2017. Identification of a Horned and Polled Bos taurus using a Gene Test. Degree of Bachelor of Agricultural Science, Lincoln University
- Chen SY, Liu LH, Fu MZ, Zhang GW, Yi J, Lai SJ, Wang W. 2017. Simultaneous introgression of three POLLED mutations into a synthetic breed of Chinese cattle. PLOS ONE 12, e0186862.
- CodonCode Corporation. 2024. www.codoncode.com . Erişim Tarihi: 22/12/2024
- Drögemüller C, Wöhlke A, Mömke S, Distl O. 2005. Fine mapping of the polled locus to a 1-Mb region on bovine chromosome 1q12. Mamm Genome. 16, 613–620. https://doi.org/10.1007/s00335-005-0016-0 .
- Georges M, Drinkwater R, King T, Mishra A, Moore SS, Nielsen D, Sargeant LS, Sorensen A, Steele MR, Zhao X, Womack JE, Hetzel J. 1993. Microsatellite mapping of a gene affecting horn development in Bos taurus. Nat Genet, 4(2), 206-210. https://doi.org/10.1038/ng0693-206
- Götz K, Luntz B, Robeis J, Edel C, Emmerling R, Buitkamp J, Anzenberger H, Duda J. 2015. Polled Fleckvieh (Simmental) cattle - current state of the breeding program. Lives Sci, 179, 80–85.
- Grobler R, van Marle-Köster E, and Visser C. 2021. Challenges in selection and breeding of polled and scur phenotypes in beef cattle. Lives Sci, 247, p.104479.
- Kling-Eveillard F, Knierim U, Irrgang N, Gottardo F, Ricci R, Dockès AC. 2015. Attitudes of farmers towards cattle dehorning. Lives Sci, 179, 12-21.
- Kumar S, Stecher G, Suleski M, Sanderford M, Sharma S, and Tamura K. 2024. MEGA12: Molecular Evolutionary Genetics Analysis version 12 for adaptive and green computing. Molecular Biology and Evolution 41:1-9
- Mariasegaram M, Harrison BE, Bolton JA, Tier B, Henshall JM, Barendse W, and Prayaga KC. 2012. Fine‐mapping the POLL locus in B rahman cattle yields the diagnostic marker CSAFG29. Anim Genet, 43(6); 683-688.
- Medugorac I, Graf A, Grohs C, Rothammer S, Zagdsuren Y, Gladyr E, Zinovieva N, Barbieri J, Seichter D, Russ I, and Eggen A. 2017. Whole-genome analysis of introgressive hybridization and characterization of the bovine legacy of Mongolian yaks. Nat Genet, 49(3); 470-475.
- Medugorac I, Seichter D, Graf A, Russ I, Blum H, Göpel KH, Rothammer S, Förster M, and Krebs S. 2012. Bovine polledness–an autosomal dominant trait with allelic heterogeneity. PloS one, 7(6), p.e39477.
- Randhawa IA, Burns BM, McGowan MR, Porto-Neto LR, Hayes BJ, Ferretti R, Schutt KM, and Lyons RE. 2020. Optimized genetic testing for polledness in multiple breeds of cattle. G3-Genes Genom Genet, 10(2);539-544. https://doi.org/10.1534/g3.119.400866
- Rothammer S, Capitan A, Mullaart E, Seichter D, Russ I, Medugorac I. 2014. The 80-kb DNA duplication on BTA1 is the only remaining candidate mutation for the polled phenotype of Friesian origin. Genet Sel, 46:44. doi:10.1186/1297-9686-46-44
- OMIA, 2024, https://www.omia.org/OMIA001965/9913/ , Erişim Tarihi: 22/12/2024
- Schmutz SM, Marquess FLS, Berryere TG, Moker JS. 1995. DNA marker-assisted selection of the polled condition in Charolais cattle. Mamm Genome, 6(10), 710713.
- Schuster F, Aldag P, Frenzel A, Hadeler K-G, Lucas-Hahn A, Niemann H, Petersen B. 2020. CRISPR/Cas12a mediated knock-in of the Polled Celtic variant to produce a polled genotype in dairy cattle. Sci. Rep, 10(1):13570.
- Seichter D, Russ I, Rothammer S, Eder J, Förster M, & Medugorac I. 2012. SNP‐based association mapping of the polled gene in divergent cattle breeds. Anim Genet, 43(5); 595-598.
- Spurlock DM, Stock ML, Coetzee JF. 2014. The impact of 3 strategies for incorporating polled genetics into a dairy cattle breeding program on the overall herd genetic merit. Journal of Dairy Science. 1;97(8):5265-74.
- UCSC, 2025. UCSC Genome Browser Genbank. https://genome.ucsc.edu/cgi-bin/hgTracks?db=bosTau8&lastVirtModeType=default&lastVirtModeExtraState=&virtModeType=default&virtMode=0&nonVirtPosition=&position=chr1%3A1909358%2D1909509&hgsid=2319655858_gACDQZ7bXRen0DY1ySRj4OREVoc7 . Erişim tarihi: 03/01/2025.
- Utsunomiya YT, Torrecilha RBÇ, Milanesi M, Paulan SdC, Utsunomiya ATH, Garcia JF. 2019. Hornless Nellore cattle (Bos indicus) carrying a novel 110 kbç duplication variant of the polled locus. Anim Genet, 50; 187-8.
- Veterinary Genetics Laboratory. 2025, https://vgl.ucdavis.edu/test/polled-vs-horned . Erişim tarihi:03/01/2025
- Wiedemar N, Tetens J, Jagannathan V, Menoud A, Neuenschwander S, Bruggmann R, et al. (2014) Independent Polled Mutations Leading to Complex Gene Expression Differences in Cattle. PLoS ONE 9(3): e93435. https://doi.org/10.1371/journal.pone.0093435
- Zoetis. 2025, https://www2.zoetis.co.nz/products-solutions/genetics/beef/hornpoll . Erişim tarihi: 03/01/2025.
There are 32 citations in total.
Details
| Primary Language | Turkish |
|---|---|
| Subjects | Animal Science, Genetics and Biostatistics |
| Journal Section | Research Article |
| Authors | |
| Project Number | FLO-2024-3010 |
| Publication Date | July 1, 2025 |
| Submission Date | January 13, 2025 |
| Acceptance Date | March 26, 2025 |
| Published in Issue | Year 2025 Volume: 66 Issue: 1 |
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