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In Silico and Expression analysis of URG-4/URGCP Gene in Different Cancer Cells

Year 2015, Volume: 9 Issue: 2, 13 - 18, 01.07.2015

Esra Tokay Feray Köçkar

Abstract

Upregulated gene 4 (URG-4/URGCP) located in chromosome 7 (7p13), was originally suggested as one of the HBxAg upregulated genes in hepatoma cells and implicated in tumorogenesis process in different cells. In this study we analyzed URG4/URGCP expression at mRNA and protein level in various cancer cell lines namely MG-63 (Human Osteosarcoma), Saos-2 (Osteosarcoma), MCF-7 (Human Breast Carcinoma), HeLa (Human Cervical Cancer), PC3 (Human Prostate Cancer), DU-145 (Human Prostate Cancer), HT-29 (Human Colon Carcinoma), HL-60 (Human Myeloid Leukemia) and Ishikawa (Human Endometrial Adenocarcinoma). The maximum URG-4/URGCP expression was obtained in MG-63 cells (nearly 8.0 fold). It was investigated that HL-60 cells were expressed the maximum protein level of URG-4/URGCP gene (nearly two-fold). Bioinformatics analysis of URG-4/URGCP variants on the mRNA and protein sequences showed that URG-4/URGCP has 5 variants (Variant-1 (3609 bp; 922 aa), Variant-2 (3831 bp; 888 aa), Variant-3 (3036 bp; 931 aa), Variant-4 (3957 bp; 888 aa) and Variant-5 (4058 bp; 888 aa). Variant-2, -4 and -5 encode the same protein with 888 amino acids.. The expression of Variant2 and -4 of URG-4/URGCP was detected in HT-29, PC3, DU-145, MCF-7 and Hep3B cells at mRNA level using RT-PCR strategy

Keywords

Upregulated Gene 4/URGCP cancer bioinformatics

Year 2015, Volume: 9 Issue: 2, 13 - 18, 01.07.2015

Esra Tokay Feray Köçkar

Abstract

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Details

Other ID JA64FC49AB
Journal Section Research Article
Authors

Esra Tokay

Feray Köçkar

Publication Date July 1, 2015
Published in Issue Year 2015 Volume: 9 Issue: 2

Cite

APA Tokay, E., & Köçkar, F. (2015). In Silico and Expression analysis of URG-4/URGCP Gene in Different Cancer Cells. Journal of Applied Biological Sciences, 9(2), 13-18.
AMA Tokay E, Köçkar F. In Silico and Expression analysis of URG-4/URGCP Gene in Different Cancer Cells. J.appl.biol.sci. July 2015;9(2):13-18.
Chicago Tokay, Esra, and Feray Köçkar. “In Silico and Expression Analysis of URG-4/URGCP Gene in Different Cancer Cells”. Journal of Applied Biological Sciences 9, no. 2 (July 2015): 13-18.
EndNote Tokay E, Köçkar F (July 1, 2015) In Silico and Expression analysis of URG-4/URGCP Gene in Different Cancer Cells. Journal of Applied Biological Sciences 9 2 13–18.
IEEE E. Tokay and F. Köçkar, “In Silico and Expression analysis of URG-4/URGCP Gene in Different Cancer Cells”, J.appl.biol.sci., vol. 9, no. 2, pp. 13–18, 2015.
ISNAD Tokay, Esra - Köçkar, Feray. “In Silico and Expression Analysis of URG-4/URGCP Gene in Different Cancer Cells”. Journal of Applied Biological Sciences 9/2 (July 2015), 13-18.
JAMA Tokay E, Köçkar F. In Silico and Expression analysis of URG-4/URGCP Gene in Different Cancer Cells. J.appl.biol.sci. 2015;9:13–18.
MLA Tokay, Esra and Feray Köçkar. “In Silico and Expression Analysis of URG-4/URGCP Gene in Different Cancer Cells”. Journal of Applied Biological Sciences, vol. 9, no. 2, 2015, pp. 13-18.
Vancouver Tokay E, Köçkar F. In Silico and Expression analysis of URG-4/URGCP Gene in Different Cancer Cells. J.appl.biol.sci. 2015;9(2):13-8.