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Çinko Oksit Nanopartiküllerinin Ocimum basilicum L.'nin İn Vitro Kültürlerinde Büyüme ve Sekonder Metabolit Birikimi Üzerine Etkileri

Year 2025, , 37 - 60, 20.06.2025

Funda Yılmaz Baydu , Ersan Bektaş

https://doi.org/10.35206/jan.1464551

Abstract

Bu çalışmada, çinko oksit nanopartikülünün (ZnO-NP) Ocimum basilicum L. (fesleğen) sürgünlerinin in vitro büyümesi, ikincil metabolit birikimi ve antioksidan aktivitesi üzerindeki etkileri araştırılmıştır. Çeşitli ZnO-NP konsantrasyonları (5, 10, 20, 40 ve 50 mg/L) sürgün uzaması, nod ve yaprak sayıları, kardeşlenme sayısı, kök oluşum yüzdesi ve kök uzunluğu gibi büyüme parametreleri üzerindeki etkilerini değerlendirmek için uygulanmıştır. Sonuçlar, düşük ZnO-NP konsantrasyonlarının sürgün uzamasını uyardığını, yüksek konsantrasyonların ise kök gelişimini olumsuz etkilediğini göstermiştir. Metanolik ekstraktlarda toplam fenolik içerik (TPC) ve antioksidan aktivite değerlendirilmiş, köklerde saplara kıyasla daha yüksek TPC gözlenmiştir. DPPH ve CUPRAC analizleri kullanılarak değerlendirilen antioksidan kapasite, ZnO-NP konsantrasyonlarına değişken tepkiler göstermiş, köklerde 40 mg/L ZnO-NP konsantrasyonunda daha yüksek antioksidan aktivite (sırasıyla SC50: 0,77 mg/mL ve 0,512 mmol TE/g bitki) tespit edilmiştir. Ayrıca, çalışmada O. basilicum'daki ana fenolik bileşik olan rosmarinik asit içeriği analiz edilmiş ve ZnO-NP uygulamasıyla, özellikle de köklerde 40 mg/L ZnO-NP konsantrasyonunda 35,98 µg fenolik/mg bitki değeriyle birikiminin arttığı bulunmuştur. Genel olarak, bulgular ZnO-NP uygulamasının O. basilicum'da büyümeyi, ikincil metabolit birikimini ve antioksidan aktiviteyi etkilediğini, etkilerin konsantrasyona ve bitki kısmına göre değiştiğini göstermektedir.

Keywords

Ocimum basilicum ZnO nanopartikül Antioksidan aktivite Rosmarinik asit Mikroçoğaltım

References

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Effects of Zinc Oxide Nanoparticles on Growth and Secondary Metabolite Accumulation in In Vitro Culture of Ocimum basilicum L.

Year 2025, , 37 - 60, 20.06.2025

Funda Yılmaz Baydu , Ersan Bektaş

https://doi.org/10.35206/jan.1464551

Abstract

The study investigated the effects of zinc oxide nanoparticle (ZnO-NP) on the growth, secondary metabolite accumulation, and antioxidant activity of Ocimum basilicum L. (basil) shoots in vitro. Various concentrations of ZnO-NP (5, 10, 20, 40 and 50 mg/L) were applied to assess their impact on growth parameters such as shoot elongation, node and leaf numbers, shoot multiplication rate, root formation percentage, and root length. Results showed that lower ZnO-NP concentrations stimulated shoot elongation, while higher concentrations negatively affected root development. Total phenolic content (TPC) and antioxidant activity were assessed in methanolic extracts, with higher TPC observed in roots compared to stems. The antioxidant capacity, evaluated using DPPH and CUPRAC assays, showed variable responses to ZnO-NP concentrations, with higher antioxidant activity (SC50: 0.77 mg/mL and 0.512 mmol TE/g plant, respectively) detected at 40 mg/L ZnO-NP concentration in roots. Additionally, the study analyzed rosmarinic acid content, the main phenolic compound in O. basilicum, and found that its accumulation increased with ZnO-NP application, especially in roots at the 40 mg/L ZnO-NP concentration with the value of 35.98 µg phenolic/mg plant. Overall, the findings suggest that ZnO-NP application influences growth, secondary metabolite accumulation, and antioxidant activity in O. basilicum, with effects varying based on concentration and plant part.

Keywords

Ocimum basilicum ZnO nanoparticle Antioxidant activity Rosmarinic acid Micropropagation

References

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  • Anjum, S., Anjum, I., Hano, C., & Kousar, S. (2019). Advances in nanomaterials as novel elicitors of pharmacologically active plant specialized metabolites: Current status and future outlooks. RSC Adv., 9, 40404-40423.
  • Apak, R., Güçlü, K., Özyürek, M., & Karademir, S. E. (2004). Novel antioxidant capacity index for dietary polyphenols and vitamin C and E, using their cupric ion reducing capability in the presence of neocuproine: CUPRAC method. Journal of Agricultural and Food Chemistry, 52, 7970-7981.
  • Bauer, N., Kiseljak, D., & Jelaska, S. (2009). The effect of yeast extract and methyl jasmonate on rosmarinic acid accumulation in Coleus blumei hairy roots. Biol Plant, 53(4), 650-656.
  • Bektaş, E. & Sökmen, A. (2016). In vitro seed germination, plantlet growth, tuberization, and syntheticseed production of Serapias vomeracea (Burm.f.) Briq., Turkish Journal of Botany, 40(6), 584-594.
  • Bektaş, E. (2020). Changes in essential oil composition, phenylalanine ammonia lyase gene expression and rosmarinic acid content during shoot organogenesis in cytokinin-treated Satureja spicigera (C. Koch) boiss. shoots. Journal of Plant Biochemistry and Biotechnology, 29, 450-460.
  • Bektaş, E., Sahin, H., Beldüz, A. O., & Güler, H. İ. (2022). HIV-1-RT inhibition activity of Satureja spicigera (C.KOCH) BOISS. Aqueous extract and docking studies of phenolic compounds identified by RP-HPLC-DAD. Journal of Food Biochemistry, 46(4), 13921.
  • Biswas, T. (2020). Elicitor induced increased rosmarinic acid content of in vitro root cultures of Ocimum basilicum L. (sweet basil). Plant Science Today, 7(2), 157-163.
  • Briskin, D. P. (2000). Medicinal plants and phytomedicines. Linking plant biochemistry and physiology to human health. Plant Physiology, 124, 507-514.
  • Burt, S. (2004). Essential oils: their antibacterial properties and potential applications in foods-a review. Int. J. Food Microbiol, 94(3), 223-253.
  • Chamani, E., Karimi Ghalehtaki, S., Mohebodini, M., & Ghanbari, A. (2015). The effect of Zinc oxide nano particles and humic acid on morphological characters and secondary metabolite production in Lilium ledebourii Bioss. Iran J Genet Plant Breed., 4, 11-19.
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  • Costa, P., Gonçalves, S., Valentão, P., Andrade, P. B., & Romano, A. (2013). Accumulation of phenolic compounds in in vitro cultures and wild plants of Lavandula viridis L’Hér and their antioxidant and anti-cholinesterase potential. Food Chem Toxicol., 57, 69-74.
  • Costa, P., Gonçalves, S., Valentão, P., Andrade, P. B., Coelho, N., Romano, A. (2012). Thymus lotocephalus wild plants and in vitro cultures produce different profiles of phenolic compounds with antioxidant activity. Food Chem., 135(3), 1253-1260.
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There are 78 citations in total.

Details

Primary Language English
Subjects Food Engineering
Journal Section Research Articles
Authors

Funda Yılmaz Baydu 0000-0002-2128-5733

Ersan Bektaş 0000-0001-9030-6908

Publication Date June 20, 2025
Submission Date April 4, 2024
Acceptance Date September 3, 2024
Published in Issue Year 2025

Cite

APA Yılmaz Baydu, F., & Bektaş, E. (2025). Effects of Zinc Oxide Nanoparticles on Growth and Secondary Metabolite Accumulation in In Vitro Culture of Ocimum basilicum L. Journal of Apitherapy and Nature, 8(1), 37-60. https://doi.org/10.35206/jan.1464551
AMA Yılmaz Baydu F, Bektaş E. Effects of Zinc Oxide Nanoparticles on Growth and Secondary Metabolite Accumulation in In Vitro Culture of Ocimum basilicum L. J.Apit.Nat. June 2025;8(1):37-60. doi:10.35206/jan.1464551
Chicago Yılmaz Baydu, Funda, and Ersan Bektaş. “Effects of Zinc Oxide Nanoparticles on Growth and Secondary Metabolite Accumulation in In Vitro Culture of Ocimum Basilicum L”. Journal of Apitherapy and Nature 8, no. 1 (June 2025): 37-60. https://doi.org/10.35206/jan.1464551.
EndNote Yılmaz Baydu F, Bektaş E (June 1, 2025) Effects of Zinc Oxide Nanoparticles on Growth and Secondary Metabolite Accumulation in In Vitro Culture of Ocimum basilicum L. Journal of Apitherapy and Nature 8 1 37–60.
IEEE F. Yılmaz Baydu and E. Bektaş, “Effects of Zinc Oxide Nanoparticles on Growth and Secondary Metabolite Accumulation in In Vitro Culture of Ocimum basilicum L”., J.Apit.Nat., vol. 8, no. 1, pp. 37–60, 2025, doi: 10.35206/jan.1464551.
ISNAD Yılmaz Baydu, Funda - Bektaş, Ersan. “Effects of Zinc Oxide Nanoparticles on Growth and Secondary Metabolite Accumulation in In Vitro Culture of Ocimum Basilicum L”. Journal of Apitherapy and Nature 8/1 (June 2025), 37-60. https://doi.org/10.35206/jan.1464551.
JAMA Yılmaz Baydu F, Bektaş E. Effects of Zinc Oxide Nanoparticles on Growth and Secondary Metabolite Accumulation in In Vitro Culture of Ocimum basilicum L. J.Apit.Nat. 2025;8:37–60.
MLA Yılmaz Baydu, Funda and Ersan Bektaş. “Effects of Zinc Oxide Nanoparticles on Growth and Secondary Metabolite Accumulation in In Vitro Culture of Ocimum Basilicum L”. Journal of Apitherapy and Nature, vol. 8, no. 1, 2025, pp. 37-60, doi:10.35206/jan.1464551.
Vancouver Yılmaz Baydu F, Bektaş E. Effects of Zinc Oxide Nanoparticles on Growth and Secondary Metabolite Accumulation in In Vitro Culture of Ocimum basilicum L. J.Apit.Nat. 2025;8(1):37-60.

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