Research Article
Determination of Indomethacin-Induced Gastric Ulcer Healing Effects of Several Propolis Extracts and Compared with Omeprazole
Abstract
Gastric ulcers are an essential problem for both human and animal health. Several medications are used to treat peptic ulcers. Long-term use of these drugs also causes various complications. For this reason, using natural products to treat ulcers has become very popular in recent years. Propolis is an important bee product used in apitherapy, which has various biological activities due to its many active ingredients. Due to its phenolic compounds, it is effective in preventing ulcer formation and accelerating healing. Varying the propolis content according to the solvent used naturally alters its efficiency. This study aimed to compare with omeprazole and to investigate the efficacy of hydro-alcoholic, water, DMSO, and olive oil propolis extracts in a mouse model of indomethacin-induced gastric ulcer. On histopathological examination, epithelial loss, erosion, bleeding, oedema, and inflammatory cell infiltration were scored separately and concerning the overall clinical score. Olive oil and DMSO extracts of propolis were found to be almost as effective as omeprazole. In contrast, water extract was found to be weak, and hydro-alcoholic extract had no effect, which even worsened some parameters. The results showed that the ulcer healing potential of propolis could be statistically different depending on the solvent. It was also found that DMSO and olive oil extracts of propolis have the potential to heal peptic ulcers.
Keywords
Project Number
TUBİTAK 1919B012109659
References
- Abd El-Hady, F. K., El Awdan, S. A., & Ibrahim, A. M. (2013). Anti-ulcerative potential of Egyptian propolis against oxidative gastric injury induced by indomethacin in rats. Asian J Med Pharm Res, 3, 35-42.
- Boeing, T., Mejia, J. A. A., Ccana-Ccapatinta, G. V., Mariott, M., de Souza, P., Mariano, L. N. B., & de Andrade, S. F. (2021). The gastroprotective effect of red propolis extract from Northeastern Brazil and the role of its isolated compounds. Journal of Ethnopharmacology, 267, 113623.
- Boeing, T., Monteiro Magalhaes de Oliveira, B., Aldana‐Mejía, J. A., Vidal Ccana‐Ccapatinta, G., Venzon, L., Judah Cury, B., & da Silva, L. (2023). Brazilian red propolis accelerates gastric healing and reduces gastric submucosal layer inflammation in ultrasound‐monitored rats. Chemistry & Biodiversity, 20(1).
- Costa, P., Somensi, L. B., da Silva, R. d. C. M. V. d. A. F., Mariano, L. N. B., Boeing, T., Longo, B., & Pellizzon, C. H. (2020). Role of the antioxidant properties in the gastroprotective and gastric healing activity promoted by Brazilian green propolis and the healing efficacy of Artepillin C. Inflammopharmacology, 28, 1009-1025.
- Mendonça, M. A. D., Ribeiro, A. R., Lima, A. K. D., Bezerra, G. B., Pinheiro, M. S., Albuquerque-Júnior, R. L., Gomez M. Z…..Cardoso, J. C. (2020). Red propolis and its dyslipidemic regulator formononetin: Evaluation of antioxidant activity and gastroprotective effects in rat model of gastric ulcer. Nutrients, 12(10), 2951.
- Huang, S., Zhang, C.-P., Wang, K., Li, G. Q., & Hu, F.-L. (2014). Recent advances in the chemical composition of propolis. Molecules, 19(12), 19610-19632.
- Khan, A. H., Dar, M. A., & Mir, M. A. (2023). Gastric ulcer: an overview. International Journal of Current Research in Physiology and Pharmacology, 1-7.
- Kekeçoğlu, M., & Sorucu, A. (2021). Determination of the effect of green extraction solvents on the phenolic acids and flavonoids of propolis. Journal of Research in Veterinary Medicine, 41(1), 49-54.
- Liu, C.-F., Lin, C.-C., Lin, M.-H., Lin, Y.-S., & Lin, S.-C. (2002). Cytoprotection by propolis ethanol extract of acute absolute ethanol-induced gastric mucosal lesions. The American Journal of Chinese Medicine, 30(02n03), 245-254.
- Martinotti, S., & Ranzato, E. (2015). Propolis: a new frontier for wound healing? Burns & Trauma, 3, 1-7. Mokam, C. E. D., Tamfu, A. N., Kuissu, M. M., Kucukaydin, S., Mezui, C., Enow-Orock, E. G., & Tan, P. V. (2024). Phenolic profile and chronic gastric ulcer healing effects of Cameroonian propolis. Clinical Traditional Medicine and Pharmacology, 5(2), 200140.
- Oruç, H. H., Cayci, M., Sorucu, A., Uzabaci, E., & Nyandwi, R. (2023). Characterization of commercially available propolis products in Turkey based on individual phenolic compounds. Journal of Apicultural Research, 62(5), 1225-1232.
- Oyetayo, N. S., Kodie, D. O., Nwakasi, M. I., Afolabi, O. O., Jarikre, T. A., Eyarefe, O. D., & Emikpe, B. O. (2023). Gastroprotective and ulcer healing potentials of Nigerian Bee Propolis flavonoid extract on acetic acid-induced gastric ulcers in albino rats (Wistar Strains). Advances in Traditional Medicine, 23(4), 1221-1230.
- Pillai, S. I., Kandaswamy, M., & Subramanian, S. (2010). Antiulcerogenic and ulcer healing effects of Indian propolis in experimental rat ulcer models. Journal of ApiProduct and ApiMedical Science, 2(1), 21-28.
- Pujirahayu, N., Ritonga, H., & Uslinawaty, Z. (2014). Properties and flavonoids content in propolis of some extraction method of raw propolis. International Journal of Pharmacy and Pharmaceutical Sciences, 6(6), 338-40.
- RaviKKumar, V., Rathi, S., Singh, S., Patel, B., Singh, S., Chaturvedi, K., & Sharma, B. (2023). A Comprehensive Review on ulcer and their treatment. Chinese Journal of Applied Physiology, e20230006.
- Ruiz-Hurtado, P. A., Garduño-Siciliano, L., Domínguez-Verano, P., Balderas-Cordero, D., Gorgua-Jiménez, G., Canales-Álvarez, O., & Rodríguez-Monroy, M. A. (2021a). Propolis and its gastroprotective effects on nsaid-induced gastric ulcer disease: A systematic review. Nutrients, 13(9), 3169.
- Ruiz-Hurtado, P. A., Garduño-Siciliano, L., Dominguez-Verano, P., Martinez-Galero, E., Canales-Martinez, M. M., & Rodriguez-Monroy, M. A. (2021b). Evaluation of the gastroprotective effects of Chihuahua propolis on indomethacin-induced gastric ulcers in mouse. Biomedicine & Pharmacotherapy, 137, 111345.
- Sahin, H., Kaltalioglu, K., Erisgin, Z., Coskun‐Cevher, S., & Kolayli, S. (2023). Comparison of protective effect of aqueous and ethanolic extracts of propolis against gastric ulceration in rats. Chemistry & Biodiversity, 20(5), e202300305.
- Serafim, C., Araruna, M. E., Júnior, E. A., Diniz, M., Hiruma-Lima, C., & Batista, L. (2020). A review of the role of flavonoids in peptic ulcer (2010–2020). Molecules, 25(22), 5431.
- Silva, L. M. d., Souza, P. d., Jaouni, S. K. A., Harakeh, S., Golbabapour, S., & De Andrade, S. F. (2018). Propolis and its potential to treat gastrointestinal disorders. Evidence‐Based Complementary and Alternative Medicine, 2018(1), 2035820.
- Simone-Finstrom, M., & Spivak, M. (2010). Propolis and bee health: the natural history and significance of resin use by honey bees. Apidologie, 41(3), 295-311.
- Sorucu, A., & Ceylan, Ö. (2021). Determination of antimicrobial and anti-quorum sensing activities of water and ethanol extracts of propolis. Ankara Universitesi Veteriner Fakultesi Dergisi, 68(4), 373-381. doi:10.33988/auvfd.793632
- Sorucu, A., & Oruç, H. H. (2019). Determination of biologically active phenolic compounds in propolis by LC–MS/MS according to seasons and altitudes. Journal of Food Measurement and Characterization, 13, 2461-2469.
- Yang, Y., Yin, B., Lv, L., Wang, Z., He, J., Chen, Z., & Li, Y. (2017). Gastroprotective effect of aucubin against ethanol-induced gastric mucosal injury in mice. Life Sciences, 189, 44-51.
Çeşitli Propolis Ekstraktlarının İndometasin ile İndüklenen Mide Ülserine Karşı İyileştirici Etkilerinin Belirlenmesi ve Omeprazol ile Karşılaştırılması
Abstract
Gastrik ülser birçok sebeple ortaya çıkan hem insan hem de hayvan sağlığı açısında önemli bir sorundur. Gastrik ülserin medikal tedavisinde farklı ilaçlar kullanılmaktadır. Bu ilaçların uzun süreli kullanımları da çeşitli komplikasyonlar oluşturmaktadır. Bu sebeple son yıllarda ülser iyileşmesinde doğal ürünlerin kullanımı oldukça popüler hale gelmiştir. Bunların içerisinde önemli bir arı ürünü olan ve apiterapide kullanılan propolis gelmektedir. Propolis içerisinde bulundurduğu birçok aktif bileşen nedeniyle farklı biyolojik aktivitelere sahip önemli bir arı ürünüdür. Özellikle içeriğindeki fenolik bileşikler nedeniyle yara iyileşmesi hızlanması ve ülser oluşumunun engellenmesi gibi etkinlik gösterdiği belirlenmiştir. Propolis içeriğinin çözücülere bağlı değişmesi doğal olarak etkinliğinin de değişmesine neden olmaktadır. Bu çalışmanın amacı, indometazin ile indüklenen gastrik ülser fare modelinde hidroalkolik, su, DMSO ve zeytinyağı propolis ekstrelerinin etkinliğini araştırmak ve omeprazol ile karşılaştırmaktır. Histopatolojik incelemede epitel kaybı, erozyon, kanama, ödem ve inflamatuar hücre infiltrasyonu ayrı ayrı ve total klinik skor açısından değerlendirilmiştir. Propolisin zeytinyağı ve DMSO ekstraktları neredeyse omeprazol kadar etkin bulunurken su ekstraktının zayıf, alkol ekstraktının ise etkisinin olmadığı hatta bazı parametreleri daha da kötüleştirdiği belirlenmiştir. Sonuç olarak propolisin çözücüye bağlı ülser iyileştirme potansiyelin istatistiki olarak değişebileceği tespit edilmiştir. Ayrıca propolisin DMSO ve zeytinyağı ekstraktlarının gastrik ülser iyileştirmede potansiyellerinin olduğu belirlenmiştir.
Keywords
Project Number
TUBİTAK 1919B012109659
References
- Abd El-Hady, F. K., El Awdan, S. A., & Ibrahim, A. M. (2013). Anti-ulcerative potential of Egyptian propolis against oxidative gastric injury induced by indomethacin in rats. Asian J Med Pharm Res, 3, 35-42.
- Boeing, T., Mejia, J. A. A., Ccana-Ccapatinta, G. V., Mariott, M., de Souza, P., Mariano, L. N. B., & de Andrade, S. F. (2021). The gastroprotective effect of red propolis extract from Northeastern Brazil and the role of its isolated compounds. Journal of Ethnopharmacology, 267, 113623.
- Boeing, T., Monteiro Magalhaes de Oliveira, B., Aldana‐Mejía, J. A., Vidal Ccana‐Ccapatinta, G., Venzon, L., Judah Cury, B., & da Silva, L. (2023). Brazilian red propolis accelerates gastric healing and reduces gastric submucosal layer inflammation in ultrasound‐monitored rats. Chemistry & Biodiversity, 20(1).
- Costa, P., Somensi, L. B., da Silva, R. d. C. M. V. d. A. F., Mariano, L. N. B., Boeing, T., Longo, B., & Pellizzon, C. H. (2020). Role of the antioxidant properties in the gastroprotective and gastric healing activity promoted by Brazilian green propolis and the healing efficacy of Artepillin C. Inflammopharmacology, 28, 1009-1025.
- Mendonça, M. A. D., Ribeiro, A. R., Lima, A. K. D., Bezerra, G. B., Pinheiro, M. S., Albuquerque-Júnior, R. L., Gomez M. Z…..Cardoso, J. C. (2020). Red propolis and its dyslipidemic regulator formononetin: Evaluation of antioxidant activity and gastroprotective effects in rat model of gastric ulcer. Nutrients, 12(10), 2951.
- Huang, S., Zhang, C.-P., Wang, K., Li, G. Q., & Hu, F.-L. (2014). Recent advances in the chemical composition of propolis. Molecules, 19(12), 19610-19632.
- Khan, A. H., Dar, M. A., & Mir, M. A. (2023). Gastric ulcer: an overview. International Journal of Current Research in Physiology and Pharmacology, 1-7.
- Kekeçoğlu, M., & Sorucu, A. (2021). Determination of the effect of green extraction solvents on the phenolic acids and flavonoids of propolis. Journal of Research in Veterinary Medicine, 41(1), 49-54.
- Liu, C.-F., Lin, C.-C., Lin, M.-H., Lin, Y.-S., & Lin, S.-C. (2002). Cytoprotection by propolis ethanol extract of acute absolute ethanol-induced gastric mucosal lesions. The American Journal of Chinese Medicine, 30(02n03), 245-254.
- Martinotti, S., & Ranzato, E. (2015). Propolis: a new frontier for wound healing? Burns & Trauma, 3, 1-7. Mokam, C. E. D., Tamfu, A. N., Kuissu, M. M., Kucukaydin, S., Mezui, C., Enow-Orock, E. G., & Tan, P. V. (2024). Phenolic profile and chronic gastric ulcer healing effects of Cameroonian propolis. Clinical Traditional Medicine and Pharmacology, 5(2), 200140.
- Oruç, H. H., Cayci, M., Sorucu, A., Uzabaci, E., & Nyandwi, R. (2023). Characterization of commercially available propolis products in Turkey based on individual phenolic compounds. Journal of Apicultural Research, 62(5), 1225-1232.
- Oyetayo, N. S., Kodie, D. O., Nwakasi, M. I., Afolabi, O. O., Jarikre, T. A., Eyarefe, O. D., & Emikpe, B. O. (2023). Gastroprotective and ulcer healing potentials of Nigerian Bee Propolis flavonoid extract on acetic acid-induced gastric ulcers in albino rats (Wistar Strains). Advances in Traditional Medicine, 23(4), 1221-1230.
- Pillai, S. I., Kandaswamy, M., & Subramanian, S. (2010). Antiulcerogenic and ulcer healing effects of Indian propolis in experimental rat ulcer models. Journal of ApiProduct and ApiMedical Science, 2(1), 21-28.
- Pujirahayu, N., Ritonga, H., & Uslinawaty, Z. (2014). Properties and flavonoids content in propolis of some extraction method of raw propolis. International Journal of Pharmacy and Pharmaceutical Sciences, 6(6), 338-40.
- RaviKKumar, V., Rathi, S., Singh, S., Patel, B., Singh, S., Chaturvedi, K., & Sharma, B. (2023). A Comprehensive Review on ulcer and their treatment. Chinese Journal of Applied Physiology, e20230006.
- Ruiz-Hurtado, P. A., Garduño-Siciliano, L., Domínguez-Verano, P., Balderas-Cordero, D., Gorgua-Jiménez, G., Canales-Álvarez, O., & Rodríguez-Monroy, M. A. (2021a). Propolis and its gastroprotective effects on nsaid-induced gastric ulcer disease: A systematic review. Nutrients, 13(9), 3169.
- Ruiz-Hurtado, P. A., Garduño-Siciliano, L., Dominguez-Verano, P., Martinez-Galero, E., Canales-Martinez, M. M., & Rodriguez-Monroy, M. A. (2021b). Evaluation of the gastroprotective effects of Chihuahua propolis on indomethacin-induced gastric ulcers in mouse. Biomedicine & Pharmacotherapy, 137, 111345.
- Sahin, H., Kaltalioglu, K., Erisgin, Z., Coskun‐Cevher, S., & Kolayli, S. (2023). Comparison of protective effect of aqueous and ethanolic extracts of propolis against gastric ulceration in rats. Chemistry & Biodiversity, 20(5), e202300305.
- Serafim, C., Araruna, M. E., Júnior, E. A., Diniz, M., Hiruma-Lima, C., & Batista, L. (2020). A review of the role of flavonoids in peptic ulcer (2010–2020). Molecules, 25(22), 5431.
- Silva, L. M. d., Souza, P. d., Jaouni, S. K. A., Harakeh, S., Golbabapour, S., & De Andrade, S. F. (2018). Propolis and its potential to treat gastrointestinal disorders. Evidence‐Based Complementary and Alternative Medicine, 2018(1), 2035820.
- Simone-Finstrom, M., & Spivak, M. (2010). Propolis and bee health: the natural history and significance of resin use by honey bees. Apidologie, 41(3), 295-311.
- Sorucu, A., & Ceylan, Ö. (2021). Determination of antimicrobial and anti-quorum sensing activities of water and ethanol extracts of propolis. Ankara Universitesi Veteriner Fakultesi Dergisi, 68(4), 373-381. doi:10.33988/auvfd.793632
- Sorucu, A., & Oruç, H. H. (2019). Determination of biologically active phenolic compounds in propolis by LC–MS/MS according to seasons and altitudes. Journal of Food Measurement and Characterization, 13, 2461-2469.
- Yang, Y., Yin, B., Lv, L., Wang, Z., He, J., Chen, Z., & Li, Y. (2017). Gastroprotective effect of aucubin against ethanol-induced gastric mucosal injury in mice. Life Sciences, 189, 44-51.
There are 24 citations in total.
Details
| Primary Language | English |
|---|---|
| Subjects | Chemical Engineering (Other) |
| Journal Section | Research Articles |
| Authors | |
| Project Number | TUBİTAK 1919B012109659 |
| Publication Date | June 20, 2025 |
| Submission Date | March 3, 2025 |
| Acceptance Date | May 12, 2025 |
| Published in Issue | Year 2025 Volume: 8 Issue: 1 |
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