Research Article
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Does The Pregabalin Show Neuroprotection in a Head Injury Rat Model?

Year 2025, Volume: 9 Issue: 2, 368 - 373, 31.05.2025

Ömer Şahin , Fatma Karaca Kara

https://doi.org/10.30621/jbachs.1598708

Abstract

Background and Purpose: Traumatic brain injury (TBI) encompasses both primary and secondary injury mechanisms that contribute to its overall pathogenesis. Pregabalin is used in the treatment of neuropathic pain. Treatments targeting excitotoxicity may be useful in the posttraumatic period. Purpose of this study is to examine the possible neuroprotective effects of pregabalin administered in a rat model of head trauma.
Methods: 32 male Wistar rats were used. The rats were randomly assigned into four distinct groups, each consisting of eight subjects: a control group, a trauma group, and groups receiving treatment with MPSS and pregabalin. In Brain Samples, assessment of malondialdehyde (MDA), glutathione peroxidase (GPx), and superoxide dismutase (SOD) enzyme activities were evaluated.
Results: There was a notable statistical discrepancy in malondialdehyde (MDA) levels when comparing the control group with the others (P<0.05). Both the Pregabalin and MPSS groups exhibited elevated GPx and SOD levels relative to the control group, with the differences being statistically significant (P<0.05).
Conclusion: Pregabalin and MPSS protected the brain against traumatic injury by attenuating lipid peroxidation and inflammatory process

Keywords

Pregabalin Rat model Head injury Antioxidant

Ethical Statement

This study was approved by Institutional Animal Experiments Ethics Committee of Ankara Training and Research Hospital, University of Health Sciences (Date: 01.11.2024, Approval No: 0086).

References

  • Gean AD, Fischbein NJ. Head trauma. Neuroimaging Clin N Am 2010;20(4):527-56.
  • Steinmann J, Hartung B, Bostelmann R, Et al. Rupture of intracranial aneurysms in patients with blunt head trauma: Review of the literature. Clin Neurol Neurosurg 2020;199:106208.
  • Langlois JA, Rutland-Brown W, Wald MM. The epidemiology and impact of traumatic brain injury: a brief overview. J Head Trauma Rehabil 2006;21(5):375-8.
  • Chong ZZ, Li F, Maiese K. Oxidative stress in the brain: novel cellular targets that govern survival during neurodegenerative disease. Prog Neurobiol 2005;75(3):207-46.
  • Siesjö BK. Basic mechanisms of traumatic brain damage. Ann Emerg Med 1993;22(6):959-69.
  • Park E, Velumian AA, Fehlings MG. The role of excitotoxicity in secondary mechanisms of spinal cord injury: a review with an emphasis on the implications for white matter degeneration. J Neurotrauma 2004;21(6):754-74.
  • Tassone DM, Boyce E, Guyer J, Nuzum D. Pregabalin: a novel gamma-aminobutyric acid analogue in the treatment of neuropathic pain, partial-onset seizures, and anxiety disorders. Clin Ther 2007;29(1):26-48.
  • Joshi I, Taylor CP. Pregabalin action at a model synapse: binding to presynaptic calcium channel alpha2-delta subunit reduces neurotransmission in mice. Eur J Pharmacol 2006;553(1-3):82-8.
  • Shneker BF, McAuley JW. Pregabalin: a new neuromodulator with broad therapeutic indications. Ann Pharmacother 2005;39(12):2029-37.
  • Eutamene H, Coelho AM, Theodorou V, Et al. Antinociceptive effect of pregabalin in septic shock-induced rectal hypersensitivity in rats. J Pharmacol Exp Ther 2000;295(1):162-7.
  • Ha KY, Kim YH, Rhyu KW, Kwon SE. Pregabalin as a neuroprotector after spinal cord injury in rats. Eur Spine J 2008;17(6):864-72.
  • Ha KY, Carragee E, Cheng I, Kwon SE, Kim YH. Pregabalin as a neuroprotector after spinal cord injury in rats: biochemical analysis and effect on glial cells. J Korean Med Sci 2011;26(3):404-11.
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  • Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem 1951;193(1):265-75.
  • Paglia DE, Valentine WN. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med 1967;70(1):158-69.
  • Krzywinski M, Altman N. Points of significance: Nonparametric tests. Nat Methods 2014;11(5):467-8.
  • Balentine JD. Pathology of experimental spinal cord trauma. I. The necrotic lesion as a function of vascular injury. Lab Invest 1978;39(3):236-53.
  • Hall ED, Braughler JM. Central nervous system trauma and stroke. II. Physiological and pharmacological evidence for involvement of oxygen radicals and lipid peroxidation. Free Radic Biol Med 1989;6(3):303-13.
  • Sun Y. Free radicals, antioxidant enzymes, and carcinogenesis. Free Radic Biol Med 1990;8(6):583-99.
  • Church DF, Pryor WA. Free-radical chemistry of cigarette smoke and its toxicological implications. Environ Health Perspect 1985;64:111-26.
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  • Kahraman S, Düz B, Kayali H, Korkmaz A, Oter S, Aydin A, Sayal A. Effects of methylprednisolone and hyperbaric oxygen on oxidative status after experimental spinal cord injury: a comparative study in rats. Neurochem Res 2007;32(9):1547-51.
  • Braughler JM, Hall ED. Uptake and elimination of methylprednisolone from contused cat spinal cord following intravenous injection of the sodium succinate ester. J Neurosurg 1983;58(4):538-42.
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  • Kalayci M, Coskun O, Cagavi F, Kanter M, Armutcu F, Gul S, Acikgoz B. Neuroprotective effects of ebselen on experimental spinal cord injury in rats. Neurochem Res 2005;30(3):403-10.
  • Sullivan PG, Krishnamurthy S, Patel SP, Pandya JD, Rabchevsky AG. Temporal characterization of mitochondrial bioenergetics after spinal cord injury. J Neurotrauma 2007;24(6):991-9.
  • Beattie MS, Hermann GE, Rogers RC, Bresnahan JC. Cell death in models of spinal cord injury. Prog Brain Res 2002;137:37-47: 12440358.
  • Vaziri ND, Lee YS, Lin CY, Lin VW, Sindhu RK. NAD(P)H oxidase, superoxide dismutase, catalase, glutathione peroxidase and nitric oxide synthase expression in subacute spinal cord injury. Brain Res 2004;995(1):76-83.

Does The Pregabalin Show Neuroprotection in a Head Injury Rat Model?

Year 2025, Volume: 9 Issue: 2, 368 - 373, 31.05.2025

Ömer Şahin , Fatma Karaca Kara

https://doi.org/10.30621/jbachs.1598708

Abstract

References

  • Gean AD, Fischbein NJ. Head trauma. Neuroimaging Clin N Am 2010;20(4):527-56.
  • Steinmann J, Hartung B, Bostelmann R, Et al. Rupture of intracranial aneurysms in patients with blunt head trauma: Review of the literature. Clin Neurol Neurosurg 2020;199:106208.
  • Langlois JA, Rutland-Brown W, Wald MM. The epidemiology and impact of traumatic brain injury: a brief overview. J Head Trauma Rehabil 2006;21(5):375-8.
  • Chong ZZ, Li F, Maiese K. Oxidative stress in the brain: novel cellular targets that govern survival during neurodegenerative disease. Prog Neurobiol 2005;75(3):207-46.
  • Siesjö BK. Basic mechanisms of traumatic brain damage. Ann Emerg Med 1993;22(6):959-69.
  • Park E, Velumian AA, Fehlings MG. The role of excitotoxicity in secondary mechanisms of spinal cord injury: a review with an emphasis on the implications for white matter degeneration. J Neurotrauma 2004;21(6):754-74.
  • Tassone DM, Boyce E, Guyer J, Nuzum D. Pregabalin: a novel gamma-aminobutyric acid analogue in the treatment of neuropathic pain, partial-onset seizures, and anxiety disorders. Clin Ther 2007;29(1):26-48.
  • Joshi I, Taylor CP. Pregabalin action at a model synapse: binding to presynaptic calcium channel alpha2-delta subunit reduces neurotransmission in mice. Eur J Pharmacol 2006;553(1-3):82-8.
  • Shneker BF, McAuley JW. Pregabalin: a new neuromodulator with broad therapeutic indications. Ann Pharmacother 2005;39(12):2029-37.
  • Eutamene H, Coelho AM, Theodorou V, Et al. Antinociceptive effect of pregabalin in septic shock-induced rectal hypersensitivity in rats. J Pharmacol Exp Ther 2000;295(1):162-7.
  • Ha KY, Kim YH, Rhyu KW, Kwon SE. Pregabalin as a neuroprotector after spinal cord injury in rats. Eur Spine J 2008;17(6):864-72.
  • Ha KY, Carragee E, Cheng I, Kwon SE, Kim YH. Pregabalin as a neuroprotector after spinal cord injury in rats: biochemical analysis and effect on glial cells. J Korean Med Sci 2011;26(3):404-11.
  • Marmarou A, Foda MA, van den Brink W, Campbell J, Kita H, Demetriadou K. A new model of diffuse brain injury in rats. Part I: Pathophysiology and biomechanics. J Neurosurg 1994;80(2):291-300.
  • Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem 1951;193(1):265-75.
  • Paglia DE, Valentine WN. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med 1967;70(1):158-69.
  • Krzywinski M, Altman N. Points of significance: Nonparametric tests. Nat Methods 2014;11(5):467-8.
  • Balentine JD. Pathology of experimental spinal cord trauma. I. The necrotic lesion as a function of vascular injury. Lab Invest 1978;39(3):236-53.
  • Hall ED, Braughler JM. Central nervous system trauma and stroke. II. Physiological and pharmacological evidence for involvement of oxygen radicals and lipid peroxidation. Free Radic Biol Med 1989;6(3):303-13.
  • Sun Y. Free radicals, antioxidant enzymes, and carcinogenesis. Free Radic Biol Med 1990;8(6):583-99.
  • Church DF, Pryor WA. Free-radical chemistry of cigarette smoke and its toxicological implications. Environ Health Perspect 1985;64:111-26.
  • Ferrari R, Ceconi C, Curello S, Et al. Oxygen free radicals and myocardial damage: protective role of thiol-containing agents. Am J Med 199;91(3C):95S-105S.
  • Halliwell B. Reactive oxygen species and the central nervous system. J Neurochem. 1992 Nov;59(5):1609-23.
  • Kahraman S, Düz B, Kayali H, Korkmaz A, Oter S, Aydin A, Sayal A. Effects of methylprednisolone and hyperbaric oxygen on oxidative status after experimental spinal cord injury: a comparative study in rats. Neurochem Res 2007;32(9):1547-51.
  • Braughler JM, Hall ED. Uptake and elimination of methylprednisolone from contused cat spinal cord following intravenous injection of the sodium succinate ester. J Neurosurg 1983;58(4):538-42.
  • Bracken MB, Shepard MJ, Collins WF, Et al. A randomized, controlled trial of methylprednisolone or naloxone in the treatment of acute spinal-cord injury. Results of the Second National Acute Spinal Cord Injury Study. N Engl J Med 1990;322(20):1405-11.
  • Kalayci M, Coskun O, Cagavi F, Kanter M, Armutcu F, Gul S, Acikgoz B. Neuroprotective effects of ebselen on experimental spinal cord injury in rats. Neurochem Res 2005;30(3):403-10.
  • Sullivan PG, Krishnamurthy S, Patel SP, Pandya JD, Rabchevsky AG. Temporal characterization of mitochondrial bioenergetics after spinal cord injury. J Neurotrauma 2007;24(6):991-9.
  • Beattie MS, Hermann GE, Rogers RC, Bresnahan JC. Cell death in models of spinal cord injury. Prog Brain Res 2002;137:37-47: 12440358.
  • Vaziri ND, Lee YS, Lin CY, Lin VW, Sindhu RK. NAD(P)H oxidase, superoxide dismutase, catalase, glutathione peroxidase and nitric oxide synthase expression in subacute spinal cord injury. Brain Res 2004;995(1):76-83.
There are 29 citations in total.

Details

Primary Language English
Subjects Clinical Sciences (Other)
Journal Section Research Article
Authors

Ömer Şahin 0000-0001-9689-0068

Fatma Karaca Kara 0000-0002-4379-3085

Publication Date May 31, 2025
Submission Date December 9, 2024
Acceptance Date May 23, 2025
Published in Issue Year 2025 Volume: 9 Issue: 2

Cite

APA Şahin, Ö., & Karaca Kara, F. (2025). Does The Pregabalin Show Neuroprotection in a Head Injury Rat Model?. Journal of Basic and Clinical Health Sciences, 9(2), 368-373. https://doi.org/10.30621/jbachs.1598708
AMA Şahin Ö, Karaca Kara F. Does The Pregabalin Show Neuroprotection in a Head Injury Rat Model?. JBACHS. May 2025;9(2):368-373. doi:10.30621/jbachs.1598708
Chicago Şahin, Ömer, and Fatma Karaca Kara. “Does The Pregabalin Show Neuroprotection in a Head Injury Rat Model?”. Journal of Basic and Clinical Health Sciences 9, no. 2 (May 2025): 368-73. https://doi.org/10.30621/jbachs.1598708.
EndNote Şahin Ö, Karaca Kara F (May 1, 2025) Does The Pregabalin Show Neuroprotection in a Head Injury Rat Model?. Journal of Basic and Clinical Health Sciences 9 2 368–373.
IEEE Ö. Şahin and F. Karaca Kara, “Does The Pregabalin Show Neuroprotection in a Head Injury Rat Model?”, JBACHS, vol. 9, no. 2, pp. 368–373, 2025, doi: 10.30621/jbachs.1598708.
ISNAD Şahin, Ömer - Karaca Kara, Fatma. “Does The Pregabalin Show Neuroprotection in a Head Injury Rat Model?”. Journal of Basic and Clinical Health Sciences 9/2 (May 2025), 368-373. https://doi.org/10.30621/jbachs.1598708.
JAMA Şahin Ö, Karaca Kara F. Does The Pregabalin Show Neuroprotection in a Head Injury Rat Model?. JBACHS. 2025;9:368–373.
MLA Şahin, Ömer and Fatma Karaca Kara. “Does The Pregabalin Show Neuroprotection in a Head Injury Rat Model?”. Journal of Basic and Clinical Health Sciences, vol. 9, no. 2, 2025, pp. 368-73, doi:10.30621/jbachs.1598708.
Vancouver Şahin Ö, Karaca Kara F. Does The Pregabalin Show Neuroprotection in a Head Injury Rat Model?. JBACHS. 2025;9(2):368-73.
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