The Journal of Faculty of Pharmacy of Ankara University is an international medium, an open access, peer-reviewed journal for the publication of original research reports, reviews and short communications in English or Turkish on relevant developments in pharmaceutical sciences., Proceeding of scientific meetings may be published as special issues of supplements to the journal. Additionally, it contains previous and forthcoming national and international scientific meetings with social activities in pharmaceutical areas.
Research Article
Year 2025,
Volume: 49 Issue: 2, 2 - 2
Abstract
Amaç: Cota cinsi, Anthemis cinsinin alt türleri arasında sınıflandırılırken günümüzde ayrı bir cins olarak kabul edilmektedir. Türkiye'de de endemik türleri bulunan Cota cinsine ait türler literatürde diğer Asteracea bitkilerine kıyasla daha az çalışılmıştır. Bu çalışmada, Türkiye'de endemik bir tür olan C. pestalozzae Boiis. türünün fenolik içeriği ve antibakteriyel aktivitesinin araştırılması amaçlanmıştır.
Gereç ve Yöntem: C. pestalozzae Boiss. türünün toprak üstü kısmından hazırlanan metanollü ekstrenin fenolik içeriği Yüksek Performanslı Sıvı Kromatografisi (HPLC) analizi ile kalitatif ve kantitatif olarak analiz edilmiştir. Bu çalışmada, tespit edilen bileşiklerin her biri için tespit limiti ve tayin limiti hesaplanmıştır. Ayrıca ekstrenin antibakteriyel aktivitesi bazı Gram (+) ve (-) bakteri türlerine karşı araştırılmıştır.
Sonuç ve Tartışma: Yapılan çalışma, C. pestalozzae Boiss. bitkisinin toprak üstü kısmının metanollü ekstresinde klorojenik asit, 4,5-O-dikafeoil kinik asit, 3,5-O-dikafeoil kinik asit, rutin, hiperozit ve izokersetin kalitatif ve kantitatif olarak tespit edilmiştir. C. pestalozzae toprak üstü kısmı ekstresi sadece Staphylococcus aureus ATCC 43300'e (MRSA) karşı 10000 µg/ml MIC değeri ile antibakteriyel aktivite sergilemiştir. Çalışma sonuçları C. pestalozzae'nin fenolik bileşikler içerdiğini ve Gram (+) bakterilere karşı etkili olduğu göstermiştir.
References
- 1. Presti, R.M.L., Oppolzer, S., Oberprieler, C. (2010). A molecular phylogeny and a revised classification of the Mediterranean genus Anthemis sl (Compositae, Anthemideae) based on three molecular markers and micromorphological characters. Taxon, 59(5), 1441-1456. [CrossRef]
- 2. Özbek, M.U., Özbek, F., Vural, M. (2018). Achene morphology of the genus Cota J. Gay (Asteraceae) from Turkey and its taxonomic significance. Turkish Journal of Botany, 42(2), 208-223. [CrossRef]
- 3. Koyuncu, O., Erkara, Í.P., Yaylaci, Ö.K., Ocak, A., Osoydan, K., Özgisi, K. (2013). Biology of rediscovered rare endemic Cota fulvida (Grierson) Houlb. in Eskişehir-Turkey. Bangladesh Journal of Botany, 42(1), 91-98. [CrossRef]
- 4. Özek, G., Özbek, M.U., Arslan, M. (2018). Lipid and essential oil constituents of Cota hamzaoglui (Asteraceae). Journal of the Turkish Chemical Society Section A: Chemistry, 5(3), 1361-1370. [CrossRef]
- 5. Papaioannou, P., Lazari, D., Karioti, A., Souleles, C., Heilmann, J., Hadjipavlou-Litina, D., Skalts, H. (2007). Phenolic compounds with antioxidant activity from Anthemis tinctoria L.(Asteraceae). Zeitschrift für Naturforschung C, 62(5-6), 326-330. [CrossRef]
- 6. Shamloo, S., Jafari Marandi, S., Tajadod, G., Majd, A., Rahimi, R. (2022). Cytotoxic effect of hydroalcoholic extract of Cota tinctoria (L.) J. Gay on AGS and Hep-G2 cancer cell lines. Boletín Latinoamericano y del Caribe de Plantas Medicinales y Aromáticas, 21(1), 108-122. [CrossRef]
- 7. Özek, G., Özbek, M., Yur, S., Göger, F., Arslan, M., Özek, T. (2019). Assessment of endemic Cota fulvida (Asteraceae) for phytochemical composition and inhibitory activities against oxidation, alpha-amylase, lipoxygenase, xanthine oxidase and tyrosinase enzymes. Records of Natural Products, 13(4), 333-345. [CrossRef]
- 8. Karadeniz, A., Çinbilgel, İ., Gün, S.Ş., Çetin, A. (2015). Antioxidant activity of some Turkish medicinal plants. Natural Product Research, 29(24), 2308-2312. [CrossRef]
- 9. Göger, G., Allak, M., Şen, A., Göger, F., Tekin, M., Özek, G. (2021). Assessment of Cota altissima (L.) J. Gay for phytochemical composition and antioxidant, anti-inflammatory, antidiabetic and antimicrobial activities. Zeitschrift für Naturforschung C, 76(7-8), 317-327. [CrossRef]
- 10. Erik, İ., Bozdal, G., Şener, S.Ö., Korkmaz, B., Karaoğlu, Ş. A., Terzioğlu, S., Yayli, N. (2022). Antimicrobial and lipase inhibition of essential oil and solvent extracts of Cota tinctoria var. tinctoria and characterization of the essential oil. Turkish Journal of Chemistry, 46(4), 1234-1244. [CrossRef]
- 11. Bahadori, M.B., Zengin, G., Eskandani, M., Zali, A., Sadoughi, M.M., Ayatollahi, S.A. (2021). Determination of phenolics composition, antioxidant activity, and therapeutic potential of Golden marguerite (Cota tinctoria). Journal of Food Measurement and Characterization, 15, 3314-3322. [CrossRef]
- 12. Talhouk, R.S., Nasr, B., Fares, M.B., Ajeeb, B., Nahhas, R., Al Aaraj, L., Talhouk, S.N., Ghaddar, T.H., Saliba, N.A. (2015). Anti-inflammatory and cytostatic activities of a parthenolide‐like sesquiterpene lactone from Cota palaestina subsp. syriaca. Evidence‐Based Complementary and Alternative Medicine, 2015(1), 474597. [CrossRef]
- 13. Clinical and Laboratory Standards Institute (CLSI) Web site (2024). M07-A8 methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically; Approved Standard, Nineteenth informational supplement, USA. Retrieved date January 2009, from https://clsi.org/. Accessed date: 03.04.2024.
Year 2025,
Volume: 49 Issue: 2, 2 - 2
Abstract
Objective: The genus Cota was once considered a subgenus of Anthemis but is now recognized as an independent genus. The species belonging to Cota, including several endemic to Turkey, have been less extensively studied in the literature than other plants in the Asteraceae family. The current study aimed to examine the phenolic content and antibacterial activity of C. pestalozzae Boiss., an endemic species from Turkey.
Material and Method: The phenolic content of the methanol extract prepared from the aerial parts of C. pestalozzae Boiss. was analysed qualitatively and quantitatively using high-performance liquid chromatography (HPLC) analysis. This study calculated the limit of detection (LOD) and quantification (LOQ) for each detected compound. The antibacterial activity of the extract was also investigated against some Gram (+) and (-) bacteria strains.
Result and Discussion: In the current study, chlorogenic acid, 4,5-O-dicaffeoyl quinic acid, 3,5-O-dicaffeoyl quinic acid, rutin, hyperoside and isoquercetin were qualitatively and quantitatively detected in the methanolic extract of the aerial parts of C. pestalozzae Boiss. Extract of the aerial parts exhibited antibacterial activity only against Staphylococcus aureus ATCC 43300 (MRSA) with a MIC value of 10000 µg/ml. The research indicates that C. pestalozzae contains phenolic compounds and is effective against Gram (+) bacteria.
References
- 1. Presti, R.M.L., Oppolzer, S., Oberprieler, C. (2010). A molecular phylogeny and a revised classification of the Mediterranean genus Anthemis sl (Compositae, Anthemideae) based on three molecular markers and micromorphological characters. Taxon, 59(5), 1441-1456. [CrossRef]
- 2. Özbek, M.U., Özbek, F., Vural, M. (2018). Achene morphology of the genus Cota J. Gay (Asteraceae) from Turkey and its taxonomic significance. Turkish Journal of Botany, 42(2), 208-223. [CrossRef]
- 3. Koyuncu, O., Erkara, Í.P., Yaylaci, Ö.K., Ocak, A., Osoydan, K., Özgisi, K. (2013). Biology of rediscovered rare endemic Cota fulvida (Grierson) Houlb. in Eskişehir-Turkey. Bangladesh Journal of Botany, 42(1), 91-98. [CrossRef]
- 4. Özek, G., Özbek, M.U., Arslan, M. (2018). Lipid and essential oil constituents of Cota hamzaoglui (Asteraceae). Journal of the Turkish Chemical Society Section A: Chemistry, 5(3), 1361-1370. [CrossRef]
- 5. Papaioannou, P., Lazari, D., Karioti, A., Souleles, C., Heilmann, J., Hadjipavlou-Litina, D., Skalts, H. (2007). Phenolic compounds with antioxidant activity from Anthemis tinctoria L.(Asteraceae). Zeitschrift für Naturforschung C, 62(5-6), 326-330. [CrossRef]
- 6. Shamloo, S., Jafari Marandi, S., Tajadod, G., Majd, A., Rahimi, R. (2022). Cytotoxic effect of hydroalcoholic extract of Cota tinctoria (L.) J. Gay on AGS and Hep-G2 cancer cell lines. Boletín Latinoamericano y del Caribe de Plantas Medicinales y Aromáticas, 21(1), 108-122. [CrossRef]
- 7. Özek, G., Özbek, M., Yur, S., Göger, F., Arslan, M., Özek, T. (2019). Assessment of endemic Cota fulvida (Asteraceae) for phytochemical composition and inhibitory activities against oxidation, alpha-amylase, lipoxygenase, xanthine oxidase and tyrosinase enzymes. Records of Natural Products, 13(4), 333-345. [CrossRef]
- 8. Karadeniz, A., Çinbilgel, İ., Gün, S.Ş., Çetin, A. (2015). Antioxidant activity of some Turkish medicinal plants. Natural Product Research, 29(24), 2308-2312. [CrossRef]
- 9. Göger, G., Allak, M., Şen, A., Göger, F., Tekin, M., Özek, G. (2021). Assessment of Cota altissima (L.) J. Gay for phytochemical composition and antioxidant, anti-inflammatory, antidiabetic and antimicrobial activities. Zeitschrift für Naturforschung C, 76(7-8), 317-327. [CrossRef]
- 10. Erik, İ., Bozdal, G., Şener, S.Ö., Korkmaz, B., Karaoğlu, Ş. A., Terzioğlu, S., Yayli, N. (2022). Antimicrobial and lipase inhibition of essential oil and solvent extracts of Cota tinctoria var. tinctoria and characterization of the essential oil. Turkish Journal of Chemistry, 46(4), 1234-1244. [CrossRef]
- 11. Bahadori, M.B., Zengin, G., Eskandani, M., Zali, A., Sadoughi, M.M., Ayatollahi, S.A. (2021). Determination of phenolics composition, antioxidant activity, and therapeutic potential of Golden marguerite (Cota tinctoria). Journal of Food Measurement and Characterization, 15, 3314-3322. [CrossRef]
- 12. Talhouk, R.S., Nasr, B., Fares, M.B., Ajeeb, B., Nahhas, R., Al Aaraj, L., Talhouk, S.N., Ghaddar, T.H., Saliba, N.A. (2015). Anti-inflammatory and cytostatic activities of a parthenolide‐like sesquiterpene lactone from Cota palaestina subsp. syriaca. Evidence‐Based Complementary and Alternative Medicine, 2015(1), 474597. [CrossRef]
- 13. Clinical and Laboratory Standards Institute (CLSI) Web site (2024). M07-A8 methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically; Approved Standard, Nineteenth informational supplement, USA. Retrieved date January 2009, from https://clsi.org/. Accessed date: 03.04.2024.
There are 13 citations in total.
Details
| Primary Language | English |
|---|---|
| Subjects | Pharmacognosy |
| Journal Section | Research Article |
| Authors | |
| Early Pub Date | May 3, 2025 |
| Publication Date | |
| Submission Date | October 23, 2024 |
| Acceptance Date | December 9, 2024 |
| Published in Issue | Year 2025 Volume: 49 Issue: 2 |