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Year 2025, Volume: 29 Issue: 3, 1059 - 1066, 04.06.2025

Meliha Ekinci , Emine Esin Çalışkan , Burak Çakar , Yalçın Çelik Aydın , Derya İlem-özdemir , Yiğit Uyanıkgil , Emel Öykü Çetin Uyanıkgil

https://doi.org/10.12991/jrespharm.1694207

Abstract

References

  • [1] Siegel RL, Miller KD, Jemal A. Cancer statistics, 2020. CA Cancer J Clin. 2020; 70(1): 7–30. https://doi.org/10.3322/caac.21590.
  • [2] Ferlay J, Colombet M, Soerjomataram I, Parkin DM, Piñeros M, Znaor A, Bray F. Cancer statistics for the year 2020: An overview. Int J Cancer. 2021; 10.1002/ijc.33588. https://doi.org/10.1002/ijc.33588.
  • [3] Payolla FB, Massabni AC, Orvig C. Radiopharmaceuticals for diagnosis in nuclear medicine: A short review. Eclet Quim. 2019; 44(3): 11–19. https://doi.org/10.26850/1678-4618eqj.v44.3.2019.p11-19.
  • [4] İlem-Özdemir D, Karavana SY, Şenyiğit ZA, Çalışkan Ç, Ekinci M, Asikoglu M, Baloglu E. Radiolabeling and cell incorporation studies of gemcitabine HCl microspheres on bladder cancer and papilloma cell line. J Radioanal Nucl Chem. 2016; 310(2): 515–522. https://doi.org/10.1007/s10967-016-4805-6.
  • [5] Réti A, Barna G, Pap E, Adleff V, L Komlósi V, Jeney A, Kralovánszky J, Budai B. Enhancement of 5-fluorouracil efficacy on high COX-2 expressing HCA-7 cells by low dose indomethacin and NS-398 but not on low COX-2 expressing HT-29 cells. Pathol Oncol Res. 2009; 15(3): 335-344. https://doi.org/10.1007/s12253-008-9126-9.
  • [6] Skuli SJ, Alomari S, Gaitsch H, Bakayoko A, Skuli N, Tyler BM. Metformin and cancer, an ambiguanidous relationship. Pharmaceuticals (Basel). 2022; 15(5): 626. https://doi.org/10.3390/ph15050626.
  • [7] De Silva L, Fu JY, Htar TT, Muniyandy S, Kasbollah A, Wan Kamal WHB, Chuah LH. Characterization, optimization, and in vitro evaluation of Technetium-99m-labeled niosomes. Int J Nanomedicine. 2019; 14: 1101–1117. https://doi.org/10.2147/IJN.S184912.
  • [8] Marianecci C, Di Marzio L, Rinaldi F, Celia C, Paolino D, Alhaique F, Esposito S, Carafa M. Niosomes from 80s to present: The state of the art. Adv Colloid Interface Sci. 2014; 205: 187–206. https://doi.org/10.1016/j.cis.2013.11.018.
  • [9] Rajera R, Nagpal K, Singh SK, Mishra DN. Niosomes: A controlled and novel drug delivery system. Biol Pharm Bull. 2011; 34(7): 945–953. https://doi.org/10.1248/bpb.34.945.
  • [10] Sohrabi S, Haeri A, Mahboubi A, Mortazavi A, Dadashzadeh S. Chitosan gel-embedded moxifloxacin niosomes: An efficient antimicrobial hybrid system for burn infection. Int J Biol Macromol. 2016; 85: 625–633. https://doi.org/10.1016/j.ijbiomac.2016.01.013.
  • [11] Ekinci M, Çalışkan EE, Çakar B, İlem-Özdemir D, Uyanıkgil Y, Çetin Uyanıkgil EÖ. [99mTc]Technetium-labeled niosomes: Radiolabeling, quality control, and in vitro evaluation. ACS Omega. 2023; 8(7): 6279–6288. https://doi.org/10.1021/acsomega.2c06179.
  • [12] Monteiro LOF, Fernandes RS, Oda CMR, Lopes SC, Townsend DM, Cardoso VN, Oliveira MC, Leite EA, Rubello D, de Barros ALB. Paclitaxel-loaded folate-coated long circulating and pH-sensitive liposomes as a potential drug delivery system: A biodistribution study. Biomed Pharmacother. 2018; 97: 489–495. https://doi.org/10.1016/j.biopha.2017.10.135.
  • [13] Chen M, Liu X, Fahr A. Skin penetration and deposition of carboxyfluorescein and temoporfin from different lipid vesicular systems: In vitro study with finite and infinite dosage application. Int J Pharm. 2011; 408(1–2): 223–234. https://doi.org/10.1016/j.ijpharm.2011.02.006.
  • [14] Cho NJ, Hwang LY, Solandt JJR, Frank CW. Comparison of extruded and sonicated vesicles for planar bilayer self-assembly. Materials (Basel). 2013; 6(8): 3294–3308. https://doi.org/10.3390/ma6083294.
  • [15] Heurtault B, Saulnier P, Pech B, Proust JE, Benoit JP. Physico-chemical stability of colloidal lipid particles. Biomaterials. 2003; 24(23): 4283–300. https://doi.org/10.1016/s0142-9612(03)00331-4.
  • [16] Ekinci M, Yeǧen G, Aksu B, İlem-Özdemir D. Preparation and evaluation of poly(lactic acid)/poly(vinyl alcohol) nanoparticles using the quality by design approach. ACS Omega. 2022; 7(38): 33793-33807. https://doi.org/10.1021/acsomega.2c02141.
  • [17] Helen Sonia A, Sambath Kumar R, Ruckmani K, Gover Antoniraj M, Bhama S. Formulation and characterization of nonionic surfactant/ cholesterol niosomes for abacavir sulphate encapsulation. Int J Res Pharm Sci. 2017; 8(2): 228-238.
  • [18] Ekinci M, Ilem-Ozdemir D, Gundogdu E, Asikoglu M. Methotrexate loaded chitosan nanoparticles: Preparation, radiolabeling and in vitro evaluation for breast cancer diagnosis. J Drug Deliv Sci Technol. 2015; 30(A): 107–113. https://doi.org/10.1016/j.jddst.2015.10.004.
  • [19] Ekinci M, Santos-Oliveira R, İlem-Özdemir D. Biodistribution of 99mTc-PLA/PVA/Atezolizumab nanoparticles for non-small cell lung cancer diagnosis. Eur J Pharm Biopharm. 2022; 176: 21–31. https://doi.org/10.1016/j.ejpb.2022.05.006.
  • [20] Ekinci M, Öztürk AA, Santos-Oliveira R, İlem-Özdemir D. The use of Lamivudine-loaded PLGA nanoparticles in the diagnosis of lung cancer: Preparation, characterization, radiolabeling with 99mTc and cell binding. J Drug Deliv Sci Technol. 2022; 69: 103139. https://doi.org/10.1016/j.jddst.2022.103139.

Radiolabelling and cellular binding studies of metformin and indomethacin loaded dual niosome formulations on human colorectal adenocarcinoma cell line

Year 2025, Volume: 29 Issue: 3, 1059 - 1066, 04.06.2025

Meliha Ekinci , Emine Esin Çalışkan , Burak Çakar , Yalçın Çelik Aydın , Derya İlem-özdemir , Yiğit Uyanıkgil , Emel Öykü Çetin Uyanıkgil

https://doi.org/10.12991/jrespharm.1694207

Abstract

In the current work, film hydration was used for producing indomethacin- and metformin-loaded dual niosomes with a particle size of < 150 nm, a polydispersity index of < 0.5, and a charge of between -30 and -50 mV. [99mTc]Tc was used as a radiolabel for all niosome formulations using stannous chloride as reducing agents. Radioactive thin-layer chromatography (RTLC) was used to evaluate the niosome formulations' radiochemical purity (RP) and stability. Next, the cellular binding of reduced/hydrolyzed (R/H)-[99mTc]NaTcO4 and [99mTc]Tc-labelled niosome formulations was evaluated in human colorectal adenocarcinoma cells. Radiolabelling all niosomes with [99mTc]Tc for 15 min at 500 μg.mL–1 stannous chloride resulted in successful radiolabelling, with an observed RP of over 95%. The cell binding percentages of [99mTc]Tc-labelled niosome formulations in cancer cells were higher than those of R/H- [99mTc]NaTcO4. Finally, the recently developed dual-niosome formulations of metformin and indomethacin labelled with [99mTc]Tc may prove effective for nuclear medicine imaging.

Keywords

Niosome technetium-99m radiolabelling cell culture

References

  • [1] Siegel RL, Miller KD, Jemal A. Cancer statistics, 2020. CA Cancer J Clin. 2020; 70(1): 7–30. https://doi.org/10.3322/caac.21590.
  • [2] Ferlay J, Colombet M, Soerjomataram I, Parkin DM, Piñeros M, Znaor A, Bray F. Cancer statistics for the year 2020: An overview. Int J Cancer. 2021; 10.1002/ijc.33588. https://doi.org/10.1002/ijc.33588.
  • [3] Payolla FB, Massabni AC, Orvig C. Radiopharmaceuticals for diagnosis in nuclear medicine: A short review. Eclet Quim. 2019; 44(3): 11–19. https://doi.org/10.26850/1678-4618eqj.v44.3.2019.p11-19.
  • [4] İlem-Özdemir D, Karavana SY, Şenyiğit ZA, Çalışkan Ç, Ekinci M, Asikoglu M, Baloglu E. Radiolabeling and cell incorporation studies of gemcitabine HCl microspheres on bladder cancer and papilloma cell line. J Radioanal Nucl Chem. 2016; 310(2): 515–522. https://doi.org/10.1007/s10967-016-4805-6.
  • [5] Réti A, Barna G, Pap E, Adleff V, L Komlósi V, Jeney A, Kralovánszky J, Budai B. Enhancement of 5-fluorouracil efficacy on high COX-2 expressing HCA-7 cells by low dose indomethacin and NS-398 but not on low COX-2 expressing HT-29 cells. Pathol Oncol Res. 2009; 15(3): 335-344. https://doi.org/10.1007/s12253-008-9126-9.
  • [6] Skuli SJ, Alomari S, Gaitsch H, Bakayoko A, Skuli N, Tyler BM. Metformin and cancer, an ambiguanidous relationship. Pharmaceuticals (Basel). 2022; 15(5): 626. https://doi.org/10.3390/ph15050626.
  • [7] De Silva L, Fu JY, Htar TT, Muniyandy S, Kasbollah A, Wan Kamal WHB, Chuah LH. Characterization, optimization, and in vitro evaluation of Technetium-99m-labeled niosomes. Int J Nanomedicine. 2019; 14: 1101–1117. https://doi.org/10.2147/IJN.S184912.
  • [8] Marianecci C, Di Marzio L, Rinaldi F, Celia C, Paolino D, Alhaique F, Esposito S, Carafa M. Niosomes from 80s to present: The state of the art. Adv Colloid Interface Sci. 2014; 205: 187–206. https://doi.org/10.1016/j.cis.2013.11.018.
  • [9] Rajera R, Nagpal K, Singh SK, Mishra DN. Niosomes: A controlled and novel drug delivery system. Biol Pharm Bull. 2011; 34(7): 945–953. https://doi.org/10.1248/bpb.34.945.
  • [10] Sohrabi S, Haeri A, Mahboubi A, Mortazavi A, Dadashzadeh S. Chitosan gel-embedded moxifloxacin niosomes: An efficient antimicrobial hybrid system for burn infection. Int J Biol Macromol. 2016; 85: 625–633. https://doi.org/10.1016/j.ijbiomac.2016.01.013.
  • [11] Ekinci M, Çalışkan EE, Çakar B, İlem-Özdemir D, Uyanıkgil Y, Çetin Uyanıkgil EÖ. [99mTc]Technetium-labeled niosomes: Radiolabeling, quality control, and in vitro evaluation. ACS Omega. 2023; 8(7): 6279–6288. https://doi.org/10.1021/acsomega.2c06179.
  • [12] Monteiro LOF, Fernandes RS, Oda CMR, Lopes SC, Townsend DM, Cardoso VN, Oliveira MC, Leite EA, Rubello D, de Barros ALB. Paclitaxel-loaded folate-coated long circulating and pH-sensitive liposomes as a potential drug delivery system: A biodistribution study. Biomed Pharmacother. 2018; 97: 489–495. https://doi.org/10.1016/j.biopha.2017.10.135.
  • [13] Chen M, Liu X, Fahr A. Skin penetration and deposition of carboxyfluorescein and temoporfin from different lipid vesicular systems: In vitro study with finite and infinite dosage application. Int J Pharm. 2011; 408(1–2): 223–234. https://doi.org/10.1016/j.ijpharm.2011.02.006.
  • [14] Cho NJ, Hwang LY, Solandt JJR, Frank CW. Comparison of extruded and sonicated vesicles for planar bilayer self-assembly. Materials (Basel). 2013; 6(8): 3294–3308. https://doi.org/10.3390/ma6083294.
  • [15] Heurtault B, Saulnier P, Pech B, Proust JE, Benoit JP. Physico-chemical stability of colloidal lipid particles. Biomaterials. 2003; 24(23): 4283–300. https://doi.org/10.1016/s0142-9612(03)00331-4.
  • [16] Ekinci M, Yeǧen G, Aksu B, İlem-Özdemir D. Preparation and evaluation of poly(lactic acid)/poly(vinyl alcohol) nanoparticles using the quality by design approach. ACS Omega. 2022; 7(38): 33793-33807. https://doi.org/10.1021/acsomega.2c02141.
  • [17] Helen Sonia A, Sambath Kumar R, Ruckmani K, Gover Antoniraj M, Bhama S. Formulation and characterization of nonionic surfactant/ cholesterol niosomes for abacavir sulphate encapsulation. Int J Res Pharm Sci. 2017; 8(2): 228-238.
  • [18] Ekinci M, Ilem-Ozdemir D, Gundogdu E, Asikoglu M. Methotrexate loaded chitosan nanoparticles: Preparation, radiolabeling and in vitro evaluation for breast cancer diagnosis. J Drug Deliv Sci Technol. 2015; 30(A): 107–113. https://doi.org/10.1016/j.jddst.2015.10.004.
  • [19] Ekinci M, Santos-Oliveira R, İlem-Özdemir D. Biodistribution of 99mTc-PLA/PVA/Atezolizumab nanoparticles for non-small cell lung cancer diagnosis. Eur J Pharm Biopharm. 2022; 176: 21–31. https://doi.org/10.1016/j.ejpb.2022.05.006.
  • [20] Ekinci M, Öztürk AA, Santos-Oliveira R, İlem-Özdemir D. The use of Lamivudine-loaded PLGA nanoparticles in the diagnosis of lung cancer: Preparation, characterization, radiolabeling with 99mTc and cell binding. J Drug Deliv Sci Technol. 2022; 69: 103139. https://doi.org/10.1016/j.jddst.2022.103139.
There are 20 citations in total.

Details

Primary Language English
Subjects Pharmacology and Pharmaceutical Sciences (Other)
Journal Section Articles
Authors

Meliha Ekinci

Emine Esin Çalışkan

Burak Çakar

Yalçın Çelik Aydın

Derya İlem-özdemir

Yiğit Uyanıkgil

Emel Öykü Çetin Uyanıkgil

Publication Date June 4, 2025
Submission Date April 30, 2024
Acceptance Date July 5, 2024
Published in Issue Year 2025 Volume: 29 Issue: 3

Cite

APA Ekinci, M., Çalışkan, E. E., Çakar, B., Aydın, Y. Ç., et al. (2025). Radiolabelling and cellular binding studies of metformin and indomethacin loaded dual niosome formulations on human colorectal adenocarcinoma cell line. Journal of Research in Pharmacy, 29(3), 1059-1066. https://doi.org/10.12991/jrespharm.1694207
AMA Ekinci M, Çalışkan EE, Çakar B, Aydın YÇ, İlem-özdemir D, Uyanıkgil Y, Çetin Uyanıkgil EÖ. Radiolabelling and cellular binding studies of metformin and indomethacin loaded dual niosome formulations on human colorectal adenocarcinoma cell line. J. Res. Pharm. June 2025;29(3):1059-1066. doi:10.12991/jrespharm.1694207
Chicago Ekinci, Meliha, Emine Esin Çalışkan, Burak Çakar, Yalçın Çelik Aydın, Derya İlem-özdemir, Yiğit Uyanıkgil, and Emel Öykü Çetin Uyanıkgil. “Radiolabelling and Cellular Binding Studies of Metformin and Indomethacin Loaded Dual Niosome Formulations on Human Colorectal Adenocarcinoma Cell Line”. Journal of Research in Pharmacy 29, no. 3 (June 2025): 1059-66. https://doi.org/10.12991/jrespharm.1694207.
EndNote Ekinci M, Çalışkan EE, Çakar B, Aydın YÇ, İlem-özdemir D, Uyanıkgil Y, Çetin Uyanıkgil EÖ (June 1, 2025) Radiolabelling and cellular binding studies of metformin and indomethacin loaded dual niosome formulations on human colorectal adenocarcinoma cell line. Journal of Research in Pharmacy 29 3 1059–1066.
IEEE M. Ekinci, E. E. Çalışkan, B. Çakar, Y. Ç. Aydın, D. İlem-özdemir, Y. Uyanıkgil, and E. Ö. Çetin Uyanıkgil, “Radiolabelling and cellular binding studies of metformin and indomethacin loaded dual niosome formulations on human colorectal adenocarcinoma cell line”, J. Res. Pharm., vol. 29, no. 3, pp. 1059–1066, 2025, doi: 10.12991/jrespharm.1694207.
ISNAD Ekinci, Meliha et al. “Radiolabelling and Cellular Binding Studies of Metformin and Indomethacin Loaded Dual Niosome Formulations on Human Colorectal Adenocarcinoma Cell Line”. Journal of Research in Pharmacy 29/3 (June 2025), 1059-1066. https://doi.org/10.12991/jrespharm.1694207.
JAMA Ekinci M, Çalışkan EE, Çakar B, Aydın YÇ, İlem-özdemir D, Uyanıkgil Y, Çetin Uyanıkgil EÖ. Radiolabelling and cellular binding studies of metformin and indomethacin loaded dual niosome formulations on human colorectal adenocarcinoma cell line. J. Res. Pharm. 2025;29:1059–1066.
MLA Ekinci, Meliha et al. “Radiolabelling and Cellular Binding Studies of Metformin and Indomethacin Loaded Dual Niosome Formulations on Human Colorectal Adenocarcinoma Cell Line”. Journal of Research in Pharmacy, vol. 29, no. 3, 2025, pp. 1059-66, doi:10.12991/jrespharm.1694207.
Vancouver Ekinci M, Çalışkan EE, Çakar B, Aydın YÇ, İlem-özdemir D, Uyanıkgil Y, Çetin Uyanıkgil EÖ. Radiolabelling and cellular binding studies of metformin and indomethacin loaded dual niosome formulations on human colorectal adenocarcinoma cell line. J. Res. Pharm. 2025;29(3):1059-66.