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In vitro bacteriolytic activity of Salmonella specific novel isolated bacteriophage

Year 2024, Volume: 28 Issue: 1, 133 - 141, 28.06.2025

Hilal Basak Erol , Banu Kaşkatepe

Abstract

Salmonella is an important agent that causes foodborne gastrointestinal infections. Salmonella enterica serovar Enteritidis, serovar Typhimurium, and serovar Infantis are the main pathogenic agents of several enteric infections worldwide. Due to the emergence of multidrug resistant Salmonella strains, there is a need for new administration strategies alternative to antibiotics, such as bacteriophages, for effective control of them. The main objectives of this study were to isolate and characterize Salmonella spp. bacteriophage and compare its activity to commercial phage product and assess the effect of bacteriophages in vitro during 24-h. Because of the study, it was found that the newly isolated and named vB_SiM_12 phage could lyse 100% of the strains tested. Bacterial growth with dilutions of vB_SiM_12 phage (MOI ranging from 0.001 to 10) was measured during 24-h. The optic density values of the samples with added bacteriophage remained lower than the control sample during the 24-h incubation period. The results show that using phages may offer a promising alternative to combat biological control agents against Salmonella infection.

Keywords

Bacteriophage bacteriolytic activity invitro turbidity test Salmonella spp.

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There are 43 citations in total.

Details

Primary Language English
Subjects Pharmaceutical Microbiology
Journal Section Articles
Authors

Hilal Basak Erol 0000-0002-7943-755X

Banu Kaşkatepe 0000-0002-9722-4267

Publication Date June 28, 2025
Published in Issue Year 2024 Volume: 28 Issue: 1

Cite

APA Erol, H. B., & Kaşkatepe, B. (2025). In vitro bacteriolytic activity of Salmonella specific novel isolated bacteriophage. Journal of Research in Pharmacy, 28(1), 133-141.
AMA Erol HB, Kaşkatepe B. In vitro bacteriolytic activity of Salmonella specific novel isolated bacteriophage. J. Res. Pharm. June 2025;28(1):133-141.
Chicago Erol, Hilal Basak, and Banu Kaşkatepe. “In Vitro Bacteriolytic Activity of Salmonella Specific Novel Isolated Bacteriophage”. Journal of Research in Pharmacy 28, no. 1 (June 2025): 133-41.
EndNote Erol HB, Kaşkatepe B (June 1, 2025) In vitro bacteriolytic activity of Salmonella specific novel isolated bacteriophage. Journal of Research in Pharmacy 28 1 133–141.
IEEE H. B. Erol and B. Kaşkatepe, “In vitro bacteriolytic activity of Salmonella specific novel isolated bacteriophage”, J. Res. Pharm., vol. 28, no. 1, pp. 133–141, 2025.
ISNAD Erol, Hilal Basak - Kaşkatepe, Banu. “In Vitro Bacteriolytic Activity of Salmonella Specific Novel Isolated Bacteriophage”. Journal of Research in Pharmacy 28/1 (June 2025), 133-141.
JAMA Erol HB, Kaşkatepe B. In vitro bacteriolytic activity of Salmonella specific novel isolated bacteriophage. J. Res. Pharm. 2025;28:133–141.
MLA Erol, Hilal Basak and Banu Kaşkatepe. “In Vitro Bacteriolytic Activity of Salmonella Specific Novel Isolated Bacteriophage”. Journal of Research in Pharmacy, vol. 28, no. 1, 2025, pp. 133-41.
Vancouver Erol HB, Kaşkatepe B. In vitro bacteriolytic activity of Salmonella specific novel isolated bacteriophage. J. Res. Pharm. 2025;28(1):133-41.