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In silico PASS prediction and evaluation of antitumor and anti-angiogenic activity of peel extracts of Citrus karna Raf. fruit

Year 2024, Volume: 28 Issue: 1, 361 - 371, 28.06.2025

Prasad G. Jamkhande Mahavir H. Ghante

Abstract

Inhibition of angiogenesis is one of the significant contrivance to suppress the developing tumor. Several previous studies have shown that phytochemicals can modify numerous vital processes of tumor promotion. However, there is no literature evidence of investigation on antitumor and anti- angiogenic activity of Citrus karna Raf. fruit. It is used as folklore medicine for the treatment of various conditions like diabetic complications, gastro-intestinal disorders and neurological complication by the rural peoples in India. The present study was performed to investigate antitumor and antiangiogenic activity of Citrus Karna Raf. fruit peel. Among the different extracts, the CKME showed strong in vitro cytotoxic activity on Human Lung Cancer Cell line Hop2 and Human Hepatoma Cell line HepG2 in SRB assay. In Chick embryo chorioallantoic membrane (CAM) assay, angiogenesis processes is strongly inhibited by CKME and chrysin at 100 µg/disc. The formation of blood vessels is essential for fulfilling tumor growth requirements and transport of metastatically competent tumor cells that was reduced by CKME and chrysin. In silico PASS prediction showed that of Citrus Karna Raf. contains several phytochemicals that exhibit anticancer active by different mechanisms like inhibition of TNF expression and stimulation of apoptosis. In conclusion, the components of CKME are promising candidates for cancer treatment and further experimental investigations are needed in order to confirm their mechanism of action.

Keywords

Antitumor drug screening assays Antitumor agents Angiogenesis inhibitors CAM Assay SRB Assa ; Citrus Karna Raf.

References

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There are 24 citations in total.

Details

Primary Language English
Subjects Pharmacology and Pharmaceutical Sciences (Other)
Journal Section Articles
Authors

Prasad G. Jamkhande 0000-0003-4457-4519

Mahavir H. Ghante 0000-0002-9705-9652

Publication Date June 28, 2025
Published in Issue Year 2024 Volume: 28 Issue: 1

Cite

APA Jamkhande, P. G., & Ghante, M. H. (2025). In silico PASS prediction and evaluation of antitumor and anti-angiogenic activity of peel extracts of Citrus karna Raf. fruit. Journal of Research in Pharmacy, 28(1), 361-371.
AMA Jamkhande PG, Ghante MH. In silico PASS prediction and evaluation of antitumor and anti-angiogenic activity of peel extracts of Citrus karna Raf. fruit. J. Res. Pharm. June 2025;28(1):361-371.
Chicago Jamkhande, Prasad G., and Mahavir H. Ghante. “In Silico PASS Prediction and Evaluation of Antitumor and Anti-Angiogenic Activity of Peel Extracts of Citrus Karna Raf. Fruit”. Journal of Research in Pharmacy 28, no. 1 (June 2025): 361-71.
EndNote Jamkhande PG, Ghante MH (June 1, 2025) In silico PASS prediction and evaluation of antitumor and anti-angiogenic activity of peel extracts of Citrus karna Raf. fruit. Journal of Research in Pharmacy 28 1 361–371.
IEEE P. G. Jamkhande and M. H. Ghante, “In silico PASS prediction and evaluation of antitumor and anti-angiogenic activity of peel extracts of Citrus karna Raf. fruit”, J. Res. Pharm., vol. 28, no. 1, pp. 361–371, 2025.
ISNAD Jamkhande, Prasad G. - Ghante, Mahavir H. “In Silico PASS Prediction and Evaluation of Antitumor and Anti-Angiogenic Activity of Peel Extracts of Citrus Karna Raf. Fruit”. Journal of Research in Pharmacy 28/1 (June 2025), 361-371.
JAMA Jamkhande PG, Ghante MH. In silico PASS prediction and evaluation of antitumor and anti-angiogenic activity of peel extracts of Citrus karna Raf. fruit. J. Res. Pharm. 2025;28:361–371.
MLA Jamkhande, Prasad G. and Mahavir H. Ghante. “In Silico PASS Prediction and Evaluation of Antitumor and Anti-Angiogenic Activity of Peel Extracts of Citrus Karna Raf. Fruit”. Journal of Research in Pharmacy, vol. 28, no. 1, 2025, pp. 361-7.
Vancouver Jamkhande PG, Ghante MH. In silico PASS prediction and evaluation of antitumor and anti-angiogenic activity of peel extracts of Citrus karna Raf. fruit. J. Res. Pharm. 2025;28(1):361-7.