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Cerium oxide nanoparticles sensitize non-small lung cancer cell to ionizing radiation

Year 2018, Volume: 22 Issue: 2, 307 - 313, 27.06.2025

Shima Azizi Arash Ghasemi Hossein Asgarian-omran Zohreh Zal Azadeh Montazeri Hooman Yazdannejat Sj Hosseinimehr

Abstract

Radiotherapy is an important strategy for cancer treatment, but resistance of tumor cells to ionizing radiation (IR) still remains a main challenging issue related to radiotherapy. The aim of this study was to evaluate the sensitizing effect of cerium oxide nanoparticles (CNPs) on non-small lung cancer (A-549) cells exposure to IR. A-549 cells were treated with CNPs and exposed to IR at dose 2 Gy. The radiosensitizing effect of CNPs was evaluated by clonogenic assay and flowcytometry. The findings of this study showed that CNPs reduced the frequencies of A-549 colony when these cells were exposed to IR. CNPs treatment prior exposure to IR significantly increased the IR-induced apoptotic incidences in A-549 cells. The present study demonstrates that CNPs to be an effective sensitizer on apoptosis and cell death induced by IR in A-549 cells.

Keywords

Cerium oxide nanoparticle nanoceria radiosensitizing apoptosis ionizing radiation radiosensitizer

References

  • [1] Salehifar E, Hosseinimehr SJ. The use of cyclooxygenase-2 inhibitors for improvement of efficacy of radiotherapy in cancers. Drug Discov Today. 2016; 21(4): 654-662.
  • [2] Hosseinimehr SJ. Trends in the development of radioprotective agents. Drug Discov Today. 2007; 12(19-20): 794-805.
  • [3] Orlowski K, Rohrer Bley C, Zimmermann M, Vuong V, Hug D, Soltermann A, Broggini-Tenzer A, Pruschy M. Dynamics of tumor hypoxia in response to patupilone and ionizing radiation. PLoS One. 2012; 7(12): e51476.
  • [4] Dayal R, Singh A, Pandey A, Mishra KP. Reactive oxygen species as mediator of tumor radiosensitivity. J Cancer Res Ther. 2014; 10(4): 811-818.
  • [5] Hosseinimehr SJ, Azadbakht M, Mousavi SM, Mahmoudzadeh A, Akhlaghpoor S. Radioprotective effects of hawthorn fruit extract against gamma irradiation in mouse bone marrow cells. J Radiat Res (Tokyo). 2007; 48(1): 63-68.
  • [6] Hosseinimehr SJ, Mahmoudzadeh A, Ahmadi A, Mohamadifar S, Akhlaghpoor S. Radioprotective effects of hesperidin against genotoxicity induced by gamma-irradiation in human lymphocytes. Mutagenesis. 2009; 24(3): 233-235.
  • [7] Linkous AG, Yazlovitskaya EM. Novel radiosensitizing anticancer therapeutics. Anticancer Res. 2012; 32(7): 2487-2499.
  • [8] Nishino K, Tanamachi K, Nakanishi Y, Ide S, Kojima S, Tanuma S, Tsukimoto M. Radiosensitizing Effect of TRPV1 Channel Inhibitors in Cancer Cells. Biol Pharm Bull. 2016; 39(7): 1224-1230.
  • [9] Provencio M, Sanchez A. Therapeutic integration of new molecule-targeted therapies with radiotherapy in lung cancer. Transl Lung Cancer Res. 2014; 3(2): 89-94.
  • [10] Jeremic B. Radiation therapy. Hematol Oncol Clin North Am. 2004; 18(1): 1-12.
  • [11] Clarke RH, Moosa S, Anzivino M, Wang Y, Floyd DH, Purow BW, Lee KS. Sustained radiosensitization of hypoxic glioma cells after oxygen pretreatment in an animal model of glioblastoma and in vitro models of tumor hypoxia. PLoS One. 2014; 9(10): e111199.
  • [12] Malik A, Sultana M, Qazi A, Qazi MH, Parveen G, Waquar S, Ashraf AB, Rasool M. Role of natural radiosensitizers and cancer cell radioresistance: An update. Anal Cell Pathol (Amst). 2016; 2016: 6146595.
  • [13] Li C, Zhao W, Liu B, Xu G, Liu L, Lv H, Shang D, Yang D, Damirin A, Zhang J. Cytotoxicity of ultrafine monodispersed nanoceria on human gastric cancer cells. J Biomed Nanotechnol. 2014; 10(7): 1231-1241.
  • [14] Xiao YF, Li JM, Wang SM, Yong X, Tang B, Jie MM, Dong H, Yang XC, Yang SM. Cerium oxide nanoparticles inhibit the migration and proliferation of gastric cancer by increasing DHX15 expression. Int J Nanomedicine. 2016; 11: 3023-3034.
  • [15] Asati A, Santra S, Kaittanis C, Perez JM. Surface-charge-dependent cell localization and cytotoxicity of cerium oxide nanoparticles. ACS Nano. 2010; 4(9): 5321-5331.
  • [16] Lin W, Huang YW, Zhou XD, Ma Y. Toxicity of cerium oxide nanoparticles in human lung cancer cells. Int J Toxicol. 2006; 25(6): 451-457.
  • [17] Pesic M, Podolski-Renic A, Stojkovic S, Matovic B, Zmejkoski D, Kojic V, Bogdanović G, Pavićević A, Mojović M, Savić A, Milenković I, Kalauzi A, Radotić K. Anti-cancer effects of cerium oxide nanoparticles and its intracellular redox activity. Chem Biol Interact. 2015; 232: 85-93.
  • [18] Rubio L, Annangi B, Vila L, Hernandez A, Marcos R. Antioxidant and anti-genotoxic properties of cerium oxide nanoparticles in a pulmonary-like cell system. Arch Toxicol. 2016; 90(2): 269-278.
  • [19] Niu J, Wang K, Kolattukudy PE. Cerium oxide nanoparticles inhibit oxidative stress and nuclear factor-kappaB activation in H9c2 cardiomyocytes exposed to cigarette smoke extract. J Pharmacol Exp Ther. 2011; 338(1): 53-61.
  • [20] Xia T, Kovochich M, Liong M, Madler L, Gilbert B, Shi H, Yeh JI, Zink JI, Nel AE. Comparison of the mechanism of toxicity of zinc oxide and cerium oxide nanoparticles based on dissolution and oxidative stress properties. ACS Nano. 2008; 2(10): 2121-2134.
  • [21] Arya A, Sethy NK, Singh SK, Das M, Bhargava K. Cerium oxide nanoparticles protect rodent lungs from hypobaric hypoxia-induced oxidative stress and inflammation. Int J Nanomedicine. 2013; 8: 4507-4520.
  • [22] Alili L, Sack M, von Montfort C, Giri S, Das S, Carroll KS, Zanger K, Seal S, Brenneisen P. Downregulation of tumor growth and invasion by redox-active nanoparticles. Antioxid Redox Signal. 2013; 19(8): 765-778.
  • [23] Gao Y, Chen K, Ma JL, Gao F. Cerium oxide nanoparticles in cancer. Onco Targets Ther. 2014; 7: 835-840.
  • [24] Mittal S, Pandey AK. Cerium oxide nanoparticles induced toxicity in human lung cells: role of ROS mediated DNA damage and apoptosis. Biomed Res Int. 2014; 2014: 891934.
  • [25] Ali D, Alarifi S, Alkahtani S, AlKahtane AA, Almalik A. Cerium Oxide nanoparticles induce oxidative stress and genotoxicity in human skin melanoma cells. Cell Biochem Biophys. 2015; 71(3): 1643-1651.
  • [26] Colon J, Hsieh N, Ferguson A, Kupelian P, Seal S, Jenkins DW, Baker CH. Cerium oxide nanoparticles protect gastrointestinal epithelium from radiation-induced damage by reduction of reactive oxygen species and upregulation of superoxide dismutase 2. Nanomedicine. 2010; 6(5): 698-705.
  • [27] Tarnuzzer RW, Colon J, Patil S, Seal S. Vacancy engineered ceria nanostructures for protection from radiation-induced cellular damage. Nano Lett. 2005; 5(12): 2573-2577.
  • [28] Madero-Visbal RA, Alvarado BE, Colon JF, Baker CH, Wason MS, Isley B, Seal S, Lee CM, Das S, Manon R. Harnessing nanoparticles to improve toxicity after head and neck radiation. Nanomedicine. 2012; 8(7): 1223-1231.
  • [29] Zhou X, Wong LL, Karakoti AS, Seal S, McGinnis JF. Nanoceria inhibit the development and promote the regression of pathologic retinal neovascularization in the Vldlr knockout mouse. PLoS One. 2011; 6(2): e16733.
  • [30] Shafaghati N, Hedayati M, Hosseinimehr SJ. Protective effects of curcumin against genotoxicity induced by 131-iodine in human cultured lymphocyte cells. Pharmacogn Mag. 2014; 10(38): 106-110.
  • [31] Javvadi P, Segan AT, Tuttle SW, Koumenis C. The chemopreventive agent curcumin is a potent radiosensitizer of human cervical tumor cells via increased reactive oxygen species production and overactivation of the mitogen-activated protein kinase pathway. Mol Pharmacol. 2008; 73(5): 1491-1501.
  • [32] Orr WS, Denbo JW, Saab KR, Ng CY, Wu J, Li K, Garner JM, Morton CL, Du Z, Pfeffer LM, Davidoff AM. Curcumin potentiates rhabdomyosarcoma radiosensitivity by suppressing NF-kappaB activity. PLoS One. 2013; 8(2): e51309.
  • [33] Hosseinimehr SJ, Hosseini SA. Radiosensitive effect of curcumin on thyroid cancer cell death induced by radioiodine-131. Interdiscip Toxicol. 2014; 7(2): 85-88.
  • [34] Kim JS, Heo K, Yi JM, Gong EJ, Yang K, Moon C, Kim SH. Genistein mitigates radiation-induced testicular injury. Phytother Res. 2012; 26(8): 1119-1125.
  • [35] Rahman Mazumder MA, Hongsprabhas P. Genistein as antioxidant and antibrowning agents in in vivo and in vitro: A review. Biomed Pharmacother. 2016; 82: 379-392.
  • [36] Shin JI, Shim JH, Kim KH, Choi HS, Kim JW, Lee HG, Kim BY, Park SN, Park OJ, Yoon DY. Sensitization of the apoptotic effect of gamma-irradiation in genistein-pretreated CaSki cervical cancer cells. J Microbiol Biotechnol. 2008; 18(3): 523-531.
  • [37] Liu X, Sun C, Liu B, Jin X, Li P, Zheng X, Zhao T, Li F, Li Q. Genistein mediates the selective radiosensitizing effect in NSCLC A549 cells via inhibiting methylation of the keap1 gene promoter region. Oncotarget. 2016; 7(19): 27267-27279.
There are 37 citations in total.

Details

Primary Language English
Subjects Radiopharmacy
Journal Section Articles
Authors

Shima Azizi

Arash Ghasemi 0000-0002-9040-6746

Hossein Asgarian-omran 0000-0003-3816-8792

Zohreh Zal

Azadeh Montazeri

Hooman Yazdannejat

Sj Hosseinimehr

Publication Date June 27, 2025
Published in Issue Year 2018 Volume: 22 Issue: 2

Cite

APA Azizi, S., Ghasemi, A., Asgarian-omran, H., Zal, Z., et al. (2025). Cerium oxide nanoparticles sensitize non-small lung cancer cell to ionizing radiation. Journal of Research in Pharmacy, 22(2), 307-313.
AMA Azizi S, Ghasemi A, Asgarian-omran H, Zal Z, Montazeri A, Yazdannejat H, Hosseinimehr S. Cerium oxide nanoparticles sensitize non-small lung cancer cell to ionizing radiation. J. Res. Pharm. June 2025;22(2):307-313.
Chicago Azizi, Shima, Arash Ghasemi, Hossein Asgarian-omran, Zohreh Zal, Azadeh Montazeri, Hooman Yazdannejat, and Sj Hosseinimehr. “Cerium Oxide Nanoparticles Sensitize Non-Small Lung Cancer Cell to Ionizing Radiation”. Journal of Research in Pharmacy 22, no. 2 (June 2025): 307-13.
EndNote Azizi S, Ghasemi A, Asgarian-omran H, Zal Z, Montazeri A, Yazdannejat H, Hosseinimehr S (June 1, 2025) Cerium oxide nanoparticles sensitize non-small lung cancer cell to ionizing radiation. Journal of Research in Pharmacy 22 2 307–313.
IEEE S. Azizi, A. Ghasemi, H. Asgarian-omran, Z. Zal, A. Montazeri, H. Yazdannejat, and S. Hosseinimehr, “Cerium oxide nanoparticles sensitize non-small lung cancer cell to ionizing radiation”, J. Res. Pharm., vol. 22, no. 2, pp. 307–313, 2025.
ISNAD Azizi, Shima et al. “Cerium Oxide Nanoparticles Sensitize Non-Small Lung Cancer Cell to Ionizing Radiation”. Journal of Research in Pharmacy 22/2 (June 2025), 307-313.
JAMA Azizi S, Ghasemi A, Asgarian-omran H, Zal Z, Montazeri A, Yazdannejat H, Hosseinimehr S. Cerium oxide nanoparticles sensitize non-small lung cancer cell to ionizing radiation. J. Res. Pharm. 2025;22:307–313.
MLA Azizi, Shima et al. “Cerium Oxide Nanoparticles Sensitize Non-Small Lung Cancer Cell to Ionizing Radiation”. Journal of Research in Pharmacy, vol. 22, no. 2, 2025, pp. 307-13.
Vancouver Azizi S, Ghasemi A, Asgarian-omran H, Zal Z, Montazeri A, Yazdannejat H, Hosseinimehr S. Cerium oxide nanoparticles sensitize non-small lung cancer cell to ionizing radiation. J. Res. Pharm. 2025;22(2):307-13.