Development and validation of Q-absorbance ratio spectrophotometric method for simultaneous estimation of ondansetron HCl and esomeprazole magnesium in bulk and formulation
Abstract
The current study describes a Q- Absorption Ratio spectrophotometric method development and validation for the simultaneous determination of esomeprazole magnesium and ondansetron hydrochloride. The concentration of the drugs was determined by using the ratio of absorbance at the iso-absorptive point (λ1 = 275 nm) and the λmax of esomeprazole magnesium (λ2 = 302 nm). This method is linear for both drugs in the range of 5–25 μg/mL at λ1 (R2 = 0.999) and at λ2 (R2 = 0.9998) for ondansetron hydrochloride, and esomeprazole magnesium found at λ1 (R2 = 0.9995) and λ2 (R2 = 0.9997). According to ICH recommendations Q2(R1), statistical analysis and recovery studies have been used to validate the analysis findings. The % Recovery was 97.78% – 100.81 % of ondansetron and 100.33% – 102.03 % of esomeprazole by the standard addition method. The method was found to be precise as the % relative standard deviation was less than 2.00 in interday and intraday precision for ondansetron and esomeprazole. The results of the current study indicated that the developed method is simple, linear, precise, accurate, and sensitive, hence may be used for quality control purposes.
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Details
| Primary Language | English |
|---|---|
| Subjects | Pharmacology and Pharmaceutical Sciences (Other) |
| Journal Section | Articles |
| Authors | |
| Publication Date | June 28, 2025 |
| Published in Issue | Year 2023 Volume: 27 Issue: 6 |