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Zingiber officinale mitigates diazinon-induced testicular toxicity via suppression of oxidative stress and apoptosis in mice model

Year 2019, Volume: 23 Issue: 2, 224 - 234, 27.06.2025

Saeed Yaghubi Beklar Maedeh Hamzeh Abbasali Karimpour Fereshteh Talebpour Amiri

Abstract

Diazinon (DZN), as an organophosphate insecticide, induces testicular toxicity through oxidative stress. Ginger, a herbal medicine, has antioxidant and anti-inflammatory properties. This study was designed to investigate the effects of ginger against DZN-induced testicular toxicity. In this experimental study, thirty-two adult male mice were randomly divided into four groups. The control group; ginger group (100 mg/kg); DZN group (10 mg/kg); ginger + DZN group. Ginger and DZN received for 30 consecutive days by gavage, and DZN has been treated one hour after receiving ginger. Sperm parameters (including motility, sperm count, sperm viability rate and morphological sperm abnormalities), biochemical (MDA and GSH), testosterone levels, histopathological and immunohistochemical assays of testis were evaluated. The results revealed that treatment with DZN caused significant damage of sperm parameters (sperm motility, count, viability rate and abnormalities), increased oxidative stress (increased MDA and decreased GSH level), significant histopathological changes and decreased Johnsen’s Score, testosterone level and increased caspase-3 immunoreactivity. Ginger preserved sperm parameters and mitigated the toxic effects of DZN. Also, treatment with ginger significantly reduced caspase-3 immunoreactivity. Our results concluded that the ginger with anti-apoptotic and antioxidant activity and with scavenging free radicals protect testis against DZN-induced toxicity.

Keywords

Organophosphate insecticide diazinon ginger oxidative stress testicular toxicity caspase-3

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There are 56 citations in total.

Details

Primary Language English
Subjects Medical Pharmacology
Journal Section Articles
Authors

Saeed Yaghubi Beklar

Maedeh Hamzeh

Abbasali Karimpour

Fereshteh Talebpour Amiri

Publication Date June 27, 2025
Published in Issue Year 2019 Volume: 23 Issue: 2

Cite

APA Yaghubi Beklar, S., Hamzeh, M., Karimpour, A., Amiri, F. T. (2025). Zingiber officinale mitigates diazinon-induced testicular toxicity via suppression of oxidative stress and apoptosis in mice model. Journal of Research in Pharmacy, 23(2), 224-234.
AMA Yaghubi Beklar S, Hamzeh M, Karimpour A, Amiri FT. Zingiber officinale mitigates diazinon-induced testicular toxicity via suppression of oxidative stress and apoptosis in mice model. J. Res. Pharm. June 2025;23(2):224-234.
Chicago Yaghubi Beklar, Saeed, Maedeh Hamzeh, Abbasali Karimpour, and Fereshteh Talebpour Amiri. “Zingiber Officinale Mitigates Diazinon-Induced Testicular Toxicity via Suppression of Oxidative Stress and Apoptosis in Mice Model”. Journal of Research in Pharmacy 23, no. 2 (June 2025): 224-34.
EndNote Yaghubi Beklar S, Hamzeh M, Karimpour A, Amiri FT (June 1, 2025) Zingiber officinale mitigates diazinon-induced testicular toxicity via suppression of oxidative stress and apoptosis in mice model. Journal of Research in Pharmacy 23 2 224–234.
IEEE S. Yaghubi Beklar, M. Hamzeh, A. Karimpour, and F. T. Amiri, “Zingiber officinale mitigates diazinon-induced testicular toxicity via suppression of oxidative stress and apoptosis in mice model”, J. Res. Pharm., vol. 23, no. 2, pp. 224–234, 2025.
ISNAD Yaghubi Beklar, Saeed et al. “Zingiber Officinale Mitigates Diazinon-Induced Testicular Toxicity via Suppression of Oxidative Stress and Apoptosis in Mice Model”. Journal of Research in Pharmacy 23/2 (June 2025), 224-234.
JAMA Yaghubi Beklar S, Hamzeh M, Karimpour A, Amiri FT. Zingiber officinale mitigates diazinon-induced testicular toxicity via suppression of oxidative stress and apoptosis in mice model. J. Res. Pharm. 2025;23:224–234.
MLA Yaghubi Beklar, Saeed et al. “Zingiber Officinale Mitigates Diazinon-Induced Testicular Toxicity via Suppression of Oxidative Stress and Apoptosis in Mice Model”. Journal of Research in Pharmacy, vol. 23, no. 2, 2025, pp. 224-3.
Vancouver Yaghubi Beklar S, Hamzeh M, Karimpour A, Amiri FT. Zingiber officinale mitigates diazinon-induced testicular toxicity via suppression of oxidative stress and apoptosis in mice model. J. Res. Pharm. 2025;23(2):224-3.