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Phytochemical constituents of ethyl acetate fraction of both roots and leaves of Sansevieria triafasciata cultivated in Iraq and assessment of its anti-proliferative effect on breast cancer cell line

Year 2024, Volume: 28 Issue: 3, 808 - 820, 28.06.2025

Rawnak Yassoub Ahmed Enas Jawad Kadhim

Abstract

Aims of study are identification, isolation, identification and determination of some type bioactive constituents presents in Sanseveria trifasciata (S.trifasciata) with assessment of the effectiveness of ethyl acetate fraction roots and leaves as antiproliferative agents against breast cancer (MDA-MB-231) cell lines. The roots and leaves of S. trifasciata were macerated in n-hexane for defatting, then the defatted components were extracted using a hot extraction technique with 85% aqueous ethanol, and the extracted parts were fractionated first with chloroform, ethyl acetate, and then n-butanol. A fraction of ethyl acetate is analyzed by analytical high performance liquid chromatography (HPLC) to determine its component. Assessment of its cytotoxic activity was performed using the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. for 24 hours, multiple concentrations of extracts are used to treat the breast cancer (MDA-MB-231) cell line. After 72 hours of treatment, media were removed and cell viability was calculated. The qualitative HPLC analysis showed that the ethyl acetate fraction of roots contains apigenin, quercetin, chlorogenic acid, luteolin, catechin, and caffeic acid but did not contain kaempferol. On the other hand, the ethyl acetate fraction of leaves contained apigenin, kaempferol, chlorogenic acid, luteolin, and catechin but does not contain quercetin. A cytotoxic study (MTT assay) showed that the IC50% for roots was 89.83 µg/ml and the IC50% for leaves was 77.6 µg/ml. Ethyl acetate extracts of S. trifasciata from both roots and leaves showed anti-breast cancer cell lines through inhibition of proliferation. However, leaves showed a more inhibitory effect at the lower concentration than roots.

Keywords

Cytotoxic effect Ethyl acetate fraction HPLC MDA-MB-231 cell line S.trifasciata

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There are 40 citations in total.

Details

Primary Language English
Subjects Pharmacognosy
Journal Section Articles
Authors

Rawnak Yassoub Ahmed 0009-0002-7696-3498

Enas Jawad Kadhim 0000-0002-0010-2512

Publication Date June 28, 2025
Published in Issue Year 2024 Volume: 28 Issue: 3

Cite

APA Ahmed, R. Y., & Kadhim, E. J. (2025). Phytochemical constituents of ethyl acetate fraction of both roots and leaves of Sansevieria triafasciata cultivated in Iraq and assessment of its anti-proliferative effect on breast cancer cell line. Journal of Research in Pharmacy, 28(3), 808-820.
AMA Ahmed RY, Kadhim EJ. Phytochemical constituents of ethyl acetate fraction of both roots and leaves of Sansevieria triafasciata cultivated in Iraq and assessment of its anti-proliferative effect on breast cancer cell line. J. Res. Pharm. June 2025;28(3):808-820.
Chicago Ahmed, Rawnak Yassoub, and Enas Jawad Kadhim. “Phytochemical Constituents of Ethyl Acetate Fraction of Both Roots and Leaves of Sansevieria Triafasciata Cultivated in Iraq and Assessment of Its Anti-Proliferative Effect on Breast Cancer Cell Line”. Journal of Research in Pharmacy 28, no. 3 (June 2025): 808-20.
EndNote Ahmed RY, Kadhim EJ (June 1, 2025) Phytochemical constituents of ethyl acetate fraction of both roots and leaves of Sansevieria triafasciata cultivated in Iraq and assessment of its anti-proliferative effect on breast cancer cell line. Journal of Research in Pharmacy 28 3 808–820.
IEEE R. Y. Ahmed and E. J. Kadhim, “Phytochemical constituents of ethyl acetate fraction of both roots and leaves of Sansevieria triafasciata cultivated in Iraq and assessment of its anti-proliferative effect on breast cancer cell line”, J. Res. Pharm., vol. 28, no. 3, pp. 808–820, 2025.
ISNAD Ahmed, Rawnak Yassoub - Kadhim, Enas Jawad. “Phytochemical Constituents of Ethyl Acetate Fraction of Both Roots and Leaves of Sansevieria Triafasciata Cultivated in Iraq and Assessment of Its Anti-Proliferative Effect on Breast Cancer Cell Line”. Journal of Research in Pharmacy 28/3 (June 2025), 808-820.
JAMA Ahmed RY, Kadhim EJ. Phytochemical constituents of ethyl acetate fraction of both roots and leaves of Sansevieria triafasciata cultivated in Iraq and assessment of its anti-proliferative effect on breast cancer cell line. J. Res. Pharm. 2025;28:808–820.
MLA Ahmed, Rawnak Yassoub and Enas Jawad Kadhim. “Phytochemical Constituents of Ethyl Acetate Fraction of Both Roots and Leaves of Sansevieria Triafasciata Cultivated in Iraq and Assessment of Its Anti-Proliferative Effect on Breast Cancer Cell Line”. Journal of Research in Pharmacy, vol. 28, no. 3, 2025, pp. 808-20.
Vancouver Ahmed RY, Kadhim EJ. Phytochemical constituents of ethyl acetate fraction of both roots and leaves of Sansevieria triafasciata cultivated in Iraq and assessment of its anti-proliferative effect on breast cancer cell line. J. Res. Pharm. 2025;28(3):808-20.