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9-Hexadecenoic Acid Rich HPLC Fraction of Pithecellobium dulce Methanolic Seed Extract Exhibits Potential Antiinflammatory Activity by Inhibiting IL-8, IL-6, and PGE2: Phytochemical Characterization, in-vitro and in-vivo Evaluation

Year 2023, Volume: 27 Issue: 5, 1734 - 1750, 28.06.2025

Babanna Yelugudari Nageshwar Mesram Pratap Reddy Karnati

Abstract

Pithecellobium dulce (Roxb.) Benth. has been used as a folk medicine due to its wide range of pharmacological applications. In light of the lack of significant studies examining the anti-inflammatory potential of seeds and their phytochemical constituents, we investigated the molecular basis behind the therapeutic efficacy of methanolic extract both in-vitro and in-vivo. The phytochemical investigation of methanolic seed extract using GC-MS analysis revealed the presence of 30 molecules of diverse classes. Further solvent fractionation followed by RP-HPLC separation demonstrated the presence of 9-Hexadecenoic acid and 8,11,14 (Z, Z, Z)-Eicosatrienoic acid and were further structurally characterized by 1H-NMR and HRMS. 9-Hexadecenoic acid-rich HPLC fraction exhibited the highest % inhibition of albumin denaturation and % reduction of IL-8 and IL-6 in HaCaT cells as well as NO and PGE-2 production in RAW267.4 cells among other test samples. In-vivo anti-inflammatory findings revealed that 9-Hexadecenoic acid-rich fraction potentially reduced rat paw edema by 84.62% at 150 µg/mL, which is superior to the diclofenac. The results of the present study revealed that the 9-hexadecenoic acid-rich fraction of methanolic seed extract of P. dulce displayed potential anti-inflammatory activity both in-vitro and in-vivo, which suggests an efficient alternative in treating a variety of ailments related to inflammation and pain.

Keywords

Pithecellobium dulce Methanolic seed extract Anti-inflammatory activity 9-hexadecenoic acid GC-MS HPLC.

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There are 38 citations in total.

Details

Primary Language English
Subjects Pharmacology and Pharmaceutical Sciences (Other)
Journal Section Articles
Authors

Babanna Yelugudari 0000-0002-3128-2982

Nageshwar Mesram

Pratap Reddy Karnati

Publication Date June 28, 2025
Published in Issue Year 2023 Volume: 27 Issue: 5

Cite

APA Yelugudari, B., Mesram, N., & Karnati, P. R. (2025). 9-Hexadecenoic Acid Rich HPLC Fraction of Pithecellobium dulce Methanolic Seed Extract Exhibits Potential Antiinflammatory Activity by Inhibiting IL-8, IL-6, and PGE2: Phytochemical Characterization, in-vitro and in-vivo Evaluation. Journal of Research in Pharmacy, 27(5), 1734-1750.
AMA Yelugudari B, Mesram N, Karnati PR. 9-Hexadecenoic Acid Rich HPLC Fraction of Pithecellobium dulce Methanolic Seed Extract Exhibits Potential Antiinflammatory Activity by Inhibiting IL-8, IL-6, and PGE2: Phytochemical Characterization, in-vitro and in-vivo Evaluation. J. Res. Pharm. July 2025;27(5):1734-1750.
Chicago Yelugudari, Babanna, Nageshwar Mesram, and Pratap Reddy Karnati. “9-Hexadecenoic Acid Rich HPLC Fraction of Pithecellobium Dulce Methanolic Seed Extract Exhibits Potential Antiinflammatory Activity by Inhibiting IL-8, IL-6, and PGE2: Phytochemical Characterization, in-Vitro and in-Vivo Evaluation”. Journal of Research in Pharmacy 27, no. 5 (July 2025): 1734-50.
EndNote Yelugudari B, Mesram N, Karnati PR (July 1, 2025) 9-Hexadecenoic Acid Rich HPLC Fraction of Pithecellobium dulce Methanolic Seed Extract Exhibits Potential Antiinflammatory Activity by Inhibiting IL-8, IL-6, and PGE2: Phytochemical Characterization, in-vitro and in-vivo Evaluation. Journal of Research in Pharmacy 27 5 1734–1750.
IEEE B. Yelugudari, N. Mesram, and P. R. Karnati, “9-Hexadecenoic Acid Rich HPLC Fraction of Pithecellobium dulce Methanolic Seed Extract Exhibits Potential Antiinflammatory Activity by Inhibiting IL-8, IL-6, and PGE2: Phytochemical Characterization, in-vitro and in-vivo Evaluation”, J. Res. Pharm., vol. 27, no. 5, pp. 1734–1750, 2025.
ISNAD Yelugudari, Babanna et al. “9-Hexadecenoic Acid Rich HPLC Fraction of Pithecellobium Dulce Methanolic Seed Extract Exhibits Potential Antiinflammatory Activity by Inhibiting IL-8, IL-6, and PGE2: Phytochemical Characterization, in-Vitro and in-Vivo Evaluation”. Journal of Research in Pharmacy 27/5 (July 2025), 1734-1750.
JAMA Yelugudari B, Mesram N, Karnati PR. 9-Hexadecenoic Acid Rich HPLC Fraction of Pithecellobium dulce Methanolic Seed Extract Exhibits Potential Antiinflammatory Activity by Inhibiting IL-8, IL-6, and PGE2: Phytochemical Characterization, in-vitro and in-vivo Evaluation. J. Res. Pharm. 2025;27:1734–1750.
MLA Yelugudari, Babanna et al. “9-Hexadecenoic Acid Rich HPLC Fraction of Pithecellobium Dulce Methanolic Seed Extract Exhibits Potential Antiinflammatory Activity by Inhibiting IL-8, IL-6, and PGE2: Phytochemical Characterization, in-Vitro and in-Vivo Evaluation”. Journal of Research in Pharmacy, vol. 27, no. 5, 2025, pp. 1734-50.
Vancouver Yelugudari B, Mesram N, Karnati PR. 9-Hexadecenoic Acid Rich HPLC Fraction of Pithecellobium dulce Methanolic Seed Extract Exhibits Potential Antiinflammatory Activity by Inhibiting IL-8, IL-6, and PGE2: Phytochemical Characterization, in-vitro and in-vivo Evaluation. J. Res. Pharm. 2025;27(5):1734-50.