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Traditional Herbal Drugs against Liver Diseases – Experimented in vitro using HepG2 Cells for Induced Steatosis

Year 2023, Volume: 27 Issue: 5, 1831 - 1847, 28.06.2025

Ayşe Esra Karadağ Mehmet Evren Okur Yağmur Özhan Hande Sipahi Fatih Demirci

Abstract

Salvia fruticosa leaves, Malva sylvestris flowers, Taraxacum officinale aerial parts, Plantago ovata seeds, Tanacetum parthenium aerial parts, and Allium sativum bulbs are documented for traditional use against hepatic disorders and different liver diseases. To evaluate herbal drug material for potential use against liver diseases, at molecular level for the efficacy linked to ethnobotanical documented data.Different herbal extracts were prepared and standardized by HPLC, according to European Pharmacopoeia. Initially 0.25 mg/mL each standardized extract was applied to oleic+palmitic acid induced fatty liver using a HepG2 cell culture model. ALT, AST, GSH, and MDA levels were comparatively analyzed, in addition to cell Nil Red staining. The highest activity for MDA reduction was observed for the A. sativum extract at 48.2% level, followed by 36.4% for M. sylvestris, and S. fruticosa extracts with 27.3% reduction, respectively. Glutathione levels increased to 59.1% when A. sativum extract was applied. M. sylvestris extract increased the glutathione levels in the medium by 49.7%; S. fruticosa extract decreased ALT levels by 53.5% and M. sylvestris extract by 38.5%, whereas the standard resveratrol reduced ALT level by 30.9%, respectively. The AST levels for M. sylvestris extract was 46.5%, compared to resveratrol by 93%. A. sativum, M. sylvestris, and S. fruticosa standard extracts showed relatively good correlation and activity where further in vivo studies should be performed.

Keywords

Ethnobotany HPLC fatty liver cell culture

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There are 50 citations in total.

Details

Primary Language English
Subjects Pharmacology and Pharmaceutical Sciences (Other)
Journal Section Articles
Authors

Ayşe Esra Karadağ

Mehmet Evren Okur 0000-0001-7706-6452

Yağmur Özhan 0000-0001-6173-3281

Hande Sipahi

Fatih Demirci

Publication Date June 28, 2025
Published in Issue Year 2023 Volume: 27 Issue: 5

Cite

APA Karadağ, A. E., Okur, M. E., Özhan, Y., Sipahi, H., et al. (2025). Traditional Herbal Drugs against Liver Diseases – Experimented in vitro using HepG2 Cells for Induced Steatosis. Journal of Research in Pharmacy, 27(5), 1831-1847.
AMA Karadağ AE, Okur ME, Özhan Y, Sipahi H, Demirci F. Traditional Herbal Drugs against Liver Diseases – Experimented in vitro using HepG2 Cells for Induced Steatosis. J. Res. Pharm. July 2025;27(5):1831-1847.
Chicago Karadağ, Ayşe Esra, Mehmet Evren Okur, Yağmur Özhan, Hande Sipahi, and Fatih Demirci. “Traditional Herbal Drugs Against Liver Diseases – Experimented in Vitro Using HepG2 Cells for Induced Steatosis”. Journal of Research in Pharmacy 27, no. 5 (July 2025): 1831-47.
EndNote Karadağ AE, Okur ME, Özhan Y, Sipahi H, Demirci F (July 1, 2025) Traditional Herbal Drugs against Liver Diseases – Experimented in vitro using HepG2 Cells for Induced Steatosis. Journal of Research in Pharmacy 27 5 1831–1847.
IEEE A. E. Karadağ, M. E. Okur, Y. Özhan, H. Sipahi, and F. Demirci, “Traditional Herbal Drugs against Liver Diseases – Experimented in vitro using HepG2 Cells for Induced Steatosis”, J. Res. Pharm., vol. 27, no. 5, pp. 1831–1847, 2025.
ISNAD Karadağ, Ayşe Esra et al. “Traditional Herbal Drugs Against Liver Diseases – Experimented in Vitro Using HepG2 Cells for Induced Steatosis”. Journal of Research in Pharmacy 27/5 (July 2025), 1831-1847.
JAMA Karadağ AE, Okur ME, Özhan Y, Sipahi H, Demirci F. Traditional Herbal Drugs against Liver Diseases – Experimented in vitro using HepG2 Cells for Induced Steatosis. J. Res. Pharm. 2025;27:1831–1847.
MLA Karadağ, Ayşe Esra et al. “Traditional Herbal Drugs Against Liver Diseases – Experimented in Vitro Using HepG2 Cells for Induced Steatosis”. Journal of Research in Pharmacy, vol. 27, no. 5, 2025, pp. 1831-47.
Vancouver Karadağ AE, Okur ME, Özhan Y, Sipahi H, Demirci F. Traditional Herbal Drugs against Liver Diseases – Experimented in vitro using HepG2 Cells for Induced Steatosis. J. Res. Pharm. 2025;27(5):1831-47.