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Characterization of the compound of longan honey from indonesia using LC-MS/MS and FTIR and the mechanism of inhibition of HEp-2 cells

Year 2023, Volume: 27 Issue: 5, 2035 - 2057, 28.06.2025

La Ode Sumarlin Achmad Tjachja Nugraha Anna Muawanah Nur Ernita Nurul Amilia

Abstract

Indonesian honey contains active compounds that have the potential as antioxidant and anticancer, especially as anticancer of the larynx through inhibition of HEp-2 cells. This study aims to determine the active compounds in longan honey and proposes the mechanism of action in inhibiting HEp-2 cells. The sample was used in the form of longan obtained from honey bee breeders in Central Java. Honey samples were extracted using methanol, and then liquid-liquid partitioning was carried out successively using n-hexane and ethyl acetate. Isolation and characterization of longan honey samples using FTIR and LC-MS/MS showed the presence of the following compounds: Xanthoxol glycosides, Santonin, Octadecanamide, Indole-3 carboxylaldehyde, 3,4-dimethoxycinnamic acid, Dimethyl esculetin, Tryptophan, O-acetyl-L- serine, D-glucose-6-phosphate, Feruloiltyramine, Lauryl diethanolamide, Taurine, 6-mercaptopurine, 3-(2,4-dichlorophenyl)-4-phenylcoumarin, 3',4'-dimethoxy-3-hydroxy-6-methylflavone and D-1-((3-carboxypropyl)amino)-1-deoxyfructose. The compounds in longan honey against HEp-2 cells is quercetin, 3,6-dimethoxycinnamic acid, phenyl coumarin, dimethyl esculetin, santonin, 6-mercaptopurine, and feruloyltyramine. The proposed mechanism of active compound in honey to inhibit HEp-2 cells in several ways including via caspase pathway and purine synthesis and other relevant mechanisms. However, this assumption needs to be tested further to obtain more precise information regarding the mechanism of inhibition of HEp-2 cells.

Keywords

HEp-2 Cells inhibition longan honey LC-MS/MS FTIR

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Details

Primary Language English
Subjects Pharmacology and Pharmaceutical Sciences (Other)
Journal Section Articles
Authors

La Ode Sumarlin 0000-0001-8400-3329

Achmad Tjachja Nugraha 0000-0001-9184-7773

Anna Muawanah 0000-0001-7345-8475

Nur Ernita 0009-0000-4248-1437

Nurul Amilia 0009-0001-2842-7423

Publication Date June 28, 2025
Published in Issue Year 2023 Volume: 27 Issue: 5

Cite

APA Sumarlin, L. O., Nugraha, A. T., Muawanah, A., Ernita, N., et al. (2025). Characterization of the compound of longan honey from indonesia using LC-MS/MS and FTIR and the mechanism of inhibition of HEp-2 cells. Journal of Research in Pharmacy, 27(5), 2035-2057.
AMA Sumarlin LO, Nugraha AT, Muawanah A, Ernita N, Amilia N. Characterization of the compound of longan honey from indonesia using LC-MS/MS and FTIR and the mechanism of inhibition of HEp-2 cells. J. Res. Pharm. July 2025;27(5):2035-2057.
Chicago Sumarlin, La Ode, Achmad Tjachja Nugraha, Anna Muawanah, Nur Ernita, and Nurul Amilia. “Characterization of the Compound of Longan Honey from Indonesia Using LC-MS/MS and FTIR and the Mechanism of Inhibition of HEp-2 Cells”. Journal of Research in Pharmacy 27, no. 5 (July 2025): 2035-57.
EndNote Sumarlin LO, Nugraha AT, Muawanah A, Ernita N, Amilia N (July 1, 2025) Characterization of the compound of longan honey from indonesia using LC-MS/MS and FTIR and the mechanism of inhibition of HEp-2 cells. Journal of Research in Pharmacy 27 5 2035–2057.
IEEE L. O. Sumarlin, A. T. Nugraha, A. Muawanah, N. Ernita, and N. Amilia, “Characterization of the compound of longan honey from indonesia using LC-MS/MS and FTIR and the mechanism of inhibition of HEp-2 cells”, J. Res. Pharm., vol. 27, no. 5, pp. 2035–2057, 2025.
ISNAD Sumarlin, La Ode et al. “Characterization of the Compound of Longan Honey from Indonesia Using LC-MS/MS and FTIR and the Mechanism of Inhibition of HEp-2 Cells”. Journal of Research in Pharmacy 27/5 (July 2025), 2035-2057.
JAMA Sumarlin LO, Nugraha AT, Muawanah A, Ernita N, Amilia N. Characterization of the compound of longan honey from indonesia using LC-MS/MS and FTIR and the mechanism of inhibition of HEp-2 cells. J. Res. Pharm. 2025;27:2035–2057.
MLA Sumarlin, La Ode et al. “Characterization of the Compound of Longan Honey from Indonesia Using LC-MS/MS and FTIR and the Mechanism of Inhibition of HEp-2 Cells”. Journal of Research in Pharmacy, vol. 27, no. 5, 2025, pp. 2035-57.
Vancouver Sumarlin LO, Nugraha AT, Muawanah A, Ernita N, Amilia N. Characterization of the compound of longan honey from indonesia using LC-MS/MS and FTIR and the mechanism of inhibition of HEp-2 cells. J. Res. Pharm. 2025;27(5):2035-57.