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Study of prebiotic potential of Terminalia chebula and development of nasal synbiotic formulation

Year 2024, Volume: 28 Issue: 4, 982 - 996, 28.06.2025

Abstract

The healthy population, including children and adults, naturally carries Lactobacilli in their nasal microflora, which probiotics can maintain. The potential benefits of probiotics and prebiotics to address nasal infections have not been thoroughly investigated. Hence, a novel reconstitutable synbiotic nasal spray dried powder (NSDP) has been proposed to protect and preserve the nasal microbiome. Several batches of NSDP were prepared by spray drying Terminalia chebula (T. chebula) with varying concentrations of carriers, including sorbitol, mannitol, and maltodextrin, to study the efficacy of the prebiotic potential of T. chebula. NSDP was evaluated for yield, LOD, micromeritic properties, size distribution by optical microscopy, laser diffraction, and surface topography by SEM. L. casei as a probiotic was added before spray drying to the optimized batch of NSDP. Probiotic-loaded NSDP was reconstituted and studied for pH, rheology, pump delivery, and viability enumeration before and after the spray drying and investigated for in-vitro efficacy by co-culture technique. Mannitol as a carrier showed parameters in a desirable range. The optimized mannitol batch yielded 37.3 %, with a moisture content of 4.6 ± 0.22 %, good flow properties, and an optimum particle size of 30.68± 4.1 µm. The pH of reconstituted optimized NSDP was about 6.2 to 6.3, with pump delivery 0.112 g/spray, and had shear thinning properties with significant results in viability and in-vitro efficacy by co-culture technique (P < 0.0001). The proposed NSDP formulation can be potentially used in restoring the nasal flora and can be further investigated for protective effects against nasal infections.

References

  • [1] Miriti DM, Muthini JM, Nyamache AK. Study of bacterial respiratory infections and antimicrobial susceptibility profile among antibiotics naive outpatients visiting Meru teaching and referral hospital, Meru County, Kenya in 2018. BMC Microbiol. 2023 Dec;23(1):172. https://doi.org/10.1186/s12866-023-02905-x
  • [2] Laino J, Villena J, Kanmani P, Kitazawa H. Immunoregulatory effects triggered by lactic acid bacteria exopolysaccharides: new insights into molecular interactions with host cells. Microorganisms. 2016;4(3):27. https://doi.org/10.3390/microorganisms4030027
  • [3] Fijan S. Microorganisms with claimed probiotic Properties: an overview of recent literature. Int J Environ Res Public Health. 2014;11(5):4745-4767. https://doi.org/10.3390/ijerph110504745
  • [4] Shaw DM, Gaerthé B, Leer RJ, Van Der Stap JG, Smittenaar C, Heijne Den Bak-Glashouwer M, Thole JE, Tielen FJ, Pouwels PH, Havenith CE. Engineering the microflora to vaccinate the mucosa: Serum immunoglobulin G responses and activated draining cervical lymph nodes following mucosal application of tetanus toxin fragment C-expressing lactobacilli. Immunology. 2000;100(4):510-518. https://doi.org/10.1046/j.1365-2567.2000.00069.x
  • [5] Picó-Monllor JA, Ruzafa-Costas B, Núñez-Delegido E, Sánchez-Pellicer P, Peris-Berraco J, Navarro-Lopez V. Selection of probiotics in the prevention of respiratory tract infections and their impact on occupational health: scoping review. Nutrients. 2021;13(12):4419. https://doi.org/10.3390/nu13124419
  • [6] Patel S, Goyal A. The current trends and future perspectives of prebiotics research: a review. 3 Biotech. 2012;2(2):115-125. https://doi.org/10.1007/s13205-012-0044-x
  • [7] Kumar GP, Arulselvan P, Kumar DS, Subramanian SP. Anti-diabetic activity of fruits of Terminalia chebula on streptozotocin induced diabetic rats. J. Health Sci. 2006;52(3):283-291. https://doi.org/10.1248/jhs.52.283
  • [8] Yang MH, Ali Z, Khan IA, Khan SI. Anti-inflammatory activity of constituents isolated from Terminalia chebula. Nat Prod Commun. 2014;9(7):1934578X1400900721. https://doi.org/10.1177/1934578X1400900721
  • [9] Lee SH, Ryu SY, Choi SU, Lee CO, No Z, Kim SK, Ahn JW. Hydrolyzable tannins and related compound having cytotoxic activity from the fruits of Terminalia chebula. Arch Pharmacal Res. 1995; 18:118-120. https://doi.org/10.1007/BF02979144
  • [10] Cheng HY, Lin TC, Yu KH, Yang CM, Lin CC. Antioxidant and free radical scavenging activities of Terminalia chebula. Biol Pharm Bull 2003;26(9):1331-1335. https://doi.org/10.1248/bpb.26.1331
  • [11] Grover IS, Bala SA. Anti-mutagenic activity of Terminalia chebula (myroblan) in Salmonella typhimurium. Indian J Exp Biol 1992;30(4):339-341.
  • [12] Kumar GP, Arulselvan P, Kumar DS, Subramanian SP. Anti-diabetic activity of fruits of Terminalia chebula on streptozotocin induced diabetic rats. J Health Sci. 2006;52(3):283-291. https://doi.org/10.1248/jhs.52.283
  • [13] Dave PP, Bhatta KH, Vaghela DB, Dhiman KS. Role of Vyaghri Haritaki Avaleha and anu Taila Nasya in the management of Dushta Pratishyaya (chronic sinusitis). Int J Ayurvedic Med. 2016;7(1):49-55. https://doi.org/10.47552/ijam.v7i1.717
  • [14] Bag A, Bhattacharyya SK, Chattopadhyay RR. The development of Terminalia chebula Retz.(Combretaceae) in clinical research. Asian Pac J Trop Biomed. 2013;3(3):244-252. https://doi.org/10.1016/S2221-1691(13)60059-3
  • [15] Zhang XR, Qiao YJ, Zhu HT, Kong QH, Wang D, Yang CR, Zhang YJ. Multiple in vitro biological effects of phenolic compounds from Terminalia chebula var. tomentella. J Ethnopharmacol. 2021;275:114135. https://doi.org/10.1016/j.jep.2021.114135.
  • [16] Usharani P, Nutalapati C, Pokuri VK, Kumar CU, Taduri G. A randomized, double-blind, placebo-, and positive-controlled clinical pilot study to evaluate the efficacy and tolerability of standardized aqueous extracts of Terminalia chebula and Terminalia bellerica in subjects with hyperuricemia. Clin Pharmacol Adv Appl. 2016; 8:51-59. https://doi.org/10.2147/cpaa.s100521.
  • [17] Suchalatha S, Devi CS. Protective effect of Terminalia chebula against experimental myocardial injury induced by isoproterenol. Indian J Exp Biol. 2004;42(2):174-178. http://nopr.niscpr.res.in/handle/123456789/23345
  • [18] Tayal S, Duggal S, Bandyopadhyay P, Aggarwal A, Tandon S, Tandon C. Cytoprotective role of the aqueous extract of Terminalia chebula on renal epithelial cells. Int Braz J Urol. 2012;38:204-214. https://doi.org/10.1590/S1677-55382012000200008
  • [19] Peterson CT, Denniston K, Chopra D. Therapeutic uses of triphala in ayurvedic medicine. J Altern Complement Med. 2017;23(8):607-614. https://doi.org/10.1089/acm.2017.0083
  • [20] Jeong SH, Jang JH, Lee YB. Drug delivery to the brain via the nasal route of administration: Exploration of key targets and major consideration factors. J Pharm Invest. 2023;53(1):119-152. https://doi.org/10.1007/s40005-022-00589-5
  • [21] Ozsoy Y, Gungor S, Cevher E. Nasal delivery of high molecular weight drugs. Molecules. 2009;14(9):3754-3779. https://doi.org/10.3390/molecules14093754
  • [22] Jokicevic K, Kiekens S, Byl E, De Boeck I, Cauwenberghs E, Lebeer S, Kiekens F. Probiotic nasal spray development by spray drying. Eur J Pharm Biopharm. 2021;159:211-220. https://doi.org/10.1016/j.ejpb.2020.11.008
  • [23] De Boeck I, Spacova I, Vanderveken OM, Lebeer S. Lactic acid bacteria as probiotics for the nose? Microb Biotechnol. 2021;14(3):859-869. https://doi.org/10.1111/1751-7915.13759
  • [24] Sultan MT, Anwar MJ, Imran M, Khalil I, Saeed F, Neelum S, Alsagaby SA, Al Abdulmonem W, Abdelgawad MA, Hussain M, El-Ghorab AH. Phytochemical profile and pro-healthy properties of Terminalia chebula: A comprehensive review. Int J Food Prop. 2023;26(1):526-551. https://doi.org/10.1080/10942912.2023.2166951
  • [25] Ibraheim HK, Madhi KS, Baqer GK, Gharban HA. Effectiveness of raw bacteriocin produced from lactic acid bacteria on biofilm of methicillin-resistant Staphylococcus aureus. Vet World. 2023;16(3):491. https://doi.org/10.14202/vetworld.2023.491-499.
  • [26] Genwali GR, Acharya PP, Rajbhandari M. Isolation of gallic acid and estimation of total phenolic content in some medicinal plants and their antioxidant activity. Nepal J Sci Technol. 2013;14(1):95-102. https://www.researchgate.net/publication/270123477
  • [27] Deshkar SS, Ahire KS, Mahore JG. In vitro and in vivo evaluation of prebiotic potential of pectin on vaginal lactobacilli. J Appl Biol Biotechnol. 2022;10(1):103-111. https://doi.org/10.7324/JABB.2021100113.
  • [28] USP C. The United States pharmacopeia-the national formulary. In United states pharmacopeial convention 2018.
There are 28 citations in total.

Details

Primary Language English
Subjects Pharmaceutical Delivery Technologies
Journal Section Articles
Authors

Sanjeevani Deshkar 0000-0002-3393-717X

Shivani Mutyalwar 0009-0003-8349-9686

Jayashri Mahore 0000-0002-5356-9466

Dheeraj Nagore 0000-0003-2672-3920

Publication Date June 28, 2025
Published in Issue Year 2024 Volume: 28 Issue: 4

Cite

APA Deshkar, S., Mutyalwar, S., Mahore, J., Nagore, D. (2025). Study of prebiotic potential of Terminalia chebula and development of nasal synbiotic formulation. Journal of Research in Pharmacy, 28(4), 982-996.
AMA Deshkar S, Mutyalwar S, Mahore J, Nagore D. Study of prebiotic potential of Terminalia chebula and development of nasal synbiotic formulation. J. Res. Pharm. July 2025;28(4):982-996.
Chicago Deshkar, Sanjeevani, Shivani Mutyalwar, Jayashri Mahore, and Dheeraj Nagore. “Study of Prebiotic Potential of Terminalia Chebula and Development of Nasal Synbiotic Formulation”. Journal of Research in Pharmacy 28, no. 4 (July 2025): 982-96.
EndNote Deshkar S, Mutyalwar S, Mahore J, Nagore D (July 1, 2025) Study of prebiotic potential of Terminalia chebula and development of nasal synbiotic formulation. Journal of Research in Pharmacy 28 4 982–996.
IEEE S. Deshkar, S. Mutyalwar, J. Mahore, and D. Nagore, “Study of prebiotic potential of Terminalia chebula and development of nasal synbiotic formulation”, J. Res. Pharm., vol. 28, no. 4, pp. 982–996, 2025.
ISNAD Deshkar, Sanjeevani et al. “Study of Prebiotic Potential of Terminalia Chebula and Development of Nasal Synbiotic Formulation”. Journal of Research in Pharmacy 28/4 (July 2025), 982-996.
JAMA Deshkar S, Mutyalwar S, Mahore J, Nagore D. Study of prebiotic potential of Terminalia chebula and development of nasal synbiotic formulation. J. Res. Pharm. 2025;28:982–996.
MLA Deshkar, Sanjeevani et al. “Study of Prebiotic Potential of Terminalia Chebula and Development of Nasal Synbiotic Formulation”. Journal of Research in Pharmacy, vol. 28, no. 4, 2025, pp. 982-96.
Vancouver Deshkar S, Mutyalwar S, Mahore J, Nagore D. Study of prebiotic potential of Terminalia chebula and development of nasal synbiotic formulation. J. Res. Pharm. 2025;28(4):982-96.