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Sub-chronic Toxicity: Biochemical and Histopathological Profiling of Cincalok Oil in Wistar Rats

Year 2024, Volume: 28 Issue: 4, 1253 - 1264, 28.06.2025

Hadi Kurniawan Inarah Fajriaty Siti Nani Nurbaeti Fajar Nugraha Fortunata Saesarria Deisberanda Dian Whidyastuti

Abstract

Cincalok is a fermented shrimp from West Kalimantan, Indonesia, which has many beneficial compounds such as omega-3 and astaxanthin. The LD50 of the cincalok oil was more than 5000 mg/ Kg BW (practically non-toxic), so there is an opportunity that cincalok can develop into a supplement. However, single and repeated doses may have different toxic potential. This study aimed to observe the sub-chronic toxicity of cincalok oil in Wistar strain rats (Rattus norvegicus L.) after 28 days of repeat doses in oral administration based on OECD 407 test guidelines. Thirty of the male and female Wistar strain rats were divided into six groups respectively that consisted of one control group of Virgin Coconut Oil (VCO) administration, three dosing groups of cincalok oil administration with dose variation of 100, 400, and 1000 mg/Kg BW, and two satellite groups of 1000 mg/Kg BW dose of cincalok oil administration and VCO control administration. The biochemistry and histopathological investigation was performed. The results showed the biochemical profiles of cincalok oil has no effect on LDL, urea, creatinine, serum glutamate oxaloacetate transaminase, and serum glutamate pyruvate transaminase blood profiles. While on total cholesterol, triglycerides, and HDL profiles, it has reversible effect. Histopathological investigation showed minimal fat degeneration with little congestion and necrosis in the liver, hypertrophy, and hypercholesterolemia in the heart, hydropic degeneration in the kidney, and no disturbance was found in the lung and spleen. Based on biochemical and histopathological result showed that cincalok had no toxic effect and potential to be developed as drug.

Keywords

Blood biochemistry Histopathology Cincalok oil Sub-chronic toxicity for 28 days Wistar rats

References

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Details

Primary Language English
Subjects Basic Pharmacology
Journal Section Articles
Authors

Hadi Kurniawan 0000-0003-3272-2422

Inarah Fajriaty 0000-0002-5575-9431

Siti Nani Nurbaeti 0000-0003-0638-0967

Fajar Nugraha 0000-0002-8511-137X

Fortunata Saesarria Deisberanda 0000-0002-8106-1870

Dian Whidyastuti 0000-0002-4846-4635

Publication Date June 28, 2025
Published in Issue Year 2024 Volume: 28 Issue: 4

Cite

APA Kurniawan, H., Fajriaty, I., Nurbaeti, S. N., Nugraha, F., et al. (2025). Sub-chronic Toxicity: Biochemical and Histopathological Profiling of Cincalok Oil in Wistar Rats. Journal of Research in Pharmacy, 28(4), 1253-1264.
AMA Kurniawan H, Fajriaty I, Nurbaeti SN, Nugraha F, Deisberanda FS, Whidyastuti D. Sub-chronic Toxicity: Biochemical and Histopathological Profiling of Cincalok Oil in Wistar Rats. J. Res. Pharm. July 2025;28(4):1253-1264.
Chicago Kurniawan, Hadi, Inarah Fajriaty, Siti Nani Nurbaeti, Fajar Nugraha, Fortunata Saesarria Deisberanda, and Dian Whidyastuti. “Sub-Chronic Toxicity: Biochemical and Histopathological Profiling of Cincalok Oil in Wistar Rats”. Journal of Research in Pharmacy 28, no. 4 (July 2025): 1253-64.
EndNote Kurniawan H, Fajriaty I, Nurbaeti SN, Nugraha F, Deisberanda FS, Whidyastuti D (July 1, 2025) Sub-chronic Toxicity: Biochemical and Histopathological Profiling of Cincalok Oil in Wistar Rats. Journal of Research in Pharmacy 28 4 1253–1264.
IEEE H. Kurniawan, I. Fajriaty, S. N. Nurbaeti, F. Nugraha, F. S. Deisberanda, and D. Whidyastuti, “Sub-chronic Toxicity: Biochemical and Histopathological Profiling of Cincalok Oil in Wistar Rats”, J. Res. Pharm., vol. 28, no. 4, pp. 1253–1264, 2025.
ISNAD Kurniawan, Hadi et al. “Sub-Chronic Toxicity: Biochemical and Histopathological Profiling of Cincalok Oil in Wistar Rats”. Journal of Research in Pharmacy 28/4 (July 2025), 1253-1264.
JAMA Kurniawan H, Fajriaty I, Nurbaeti SN, Nugraha F, Deisberanda FS, Whidyastuti D. Sub-chronic Toxicity: Biochemical and Histopathological Profiling of Cincalok Oil in Wistar Rats. J. Res. Pharm. 2025;28:1253–1264.
MLA Kurniawan, Hadi et al. “Sub-Chronic Toxicity: Biochemical and Histopathological Profiling of Cincalok Oil in Wistar Rats”. Journal of Research in Pharmacy, vol. 28, no. 4, 2025, pp. 1253-64.
Vancouver Kurniawan H, Fajriaty I, Nurbaeti SN, Nugraha F, Deisberanda FS, Whidyastuti D. Sub-chronic Toxicity: Biochemical and Histopathological Profiling of Cincalok Oil in Wistar Rats. J. Res. Pharm. 2025;28(4):1253-64.