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Development and validation of a GC-FID method to quantify thymoquinone in black cumin seed oils

Year 2019, Volume: 23 Issue: 3, 506 - 513, 27.06.2025

İlker Demirbolat , Murat Kartal , Ünal Karık

Abstract

Black cumin (Nigella sativa L.) is a very popular medicinal plant around the world. Pharmacological effects have been widely studied over the years. It has an economic importance and being cultivated in many parts of Turkey and neighbour countries. Thymoquinone (TQ) is a volatile compound found in black cumin seeds and a major compound of its essential and fixed oils which has been reported as anticancer, antidiabetic, spasmolytic, immunomodulator, bronchodilator, antimicrobial, analgesic, anti-inflammatory, antioxidant etc. The quality of black cumin seed oil depends on extraction technics (expression, super critic CO2 extraction etc.), quality of seeds, storage time and thymoquinone content. In this study we developed and validated a rapid quantification GC-FID method to determine the thymoquinone levels in seed oils which uses diethyl phthalate (DEP) as an internal standard.

Keywords

Thymoquinone gas chromatography black cumin Nigella sativa

References

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There are 19 citations in total.

Details

Primary Language English
Subjects Pharmaceutical Chemistry
Journal Section Articles
Authors

İlker Demirbolat

Murat Kartal

Ünal Karık

Publication Date June 27, 2025
Published in Issue Year 2019 Volume: 23 Issue: 3

Cite

APA Demirbolat, İ., Kartal, M., & Karık, Ü. (2025). Development and validation of a GC-FID method to quantify thymoquinone in black cumin seed oils. Journal of Research in Pharmacy, 23(3), 506-513.
AMA Demirbolat İ, Kartal M, Karık Ü. Development and validation of a GC-FID method to quantify thymoquinone in black cumin seed oils. J. Res. Pharm. June 2025;23(3):506-513.
Chicago Demirbolat, İlker, Murat Kartal, and Ünal Karık. “Development and Validation of a GC-FID Method to Quantify Thymoquinone in Black Cumin Seed Oils”. Journal of Research in Pharmacy 23, no. 3 (June 2025): 506-13.
EndNote Demirbolat İ, Kartal M, Karık Ü (June 1, 2025) Development and validation of a GC-FID method to quantify thymoquinone in black cumin seed oils. Journal of Research in Pharmacy 23 3 506–513.
IEEE İ. Demirbolat, M. Kartal, and Ü. Karık, “Development and validation of a GC-FID method to quantify thymoquinone in black cumin seed oils”, J. Res. Pharm., vol. 23, no. 3, pp. 506–513, 2025.
ISNAD Demirbolat, İlker et al. “Development and Validation of a GC-FID Method to Quantify Thymoquinone in Black Cumin Seed Oils”. Journal of Research in Pharmacy 23/3 (June 2025), 506-513.
JAMA Demirbolat İ, Kartal M, Karık Ü. Development and validation of a GC-FID method to quantify thymoquinone in black cumin seed oils. J. Res. Pharm. 2025;23:506–513.
MLA Demirbolat, İlker et al. “Development and Validation of a GC-FID Method to Quantify Thymoquinone in Black Cumin Seed Oils”. Journal of Research in Pharmacy, vol. 23, no. 3, 2025, pp. 506-13.
Vancouver Demirbolat İ, Kartal M, Karık Ü. Development and validation of a GC-FID method to quantify thymoquinone in black cumin seed oils. J. Res. Pharm. 2025;23(3):506-13.