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Methanolic extract of Eryngium creticum Lam leaves, flowers and roots: Quantification and qualification of phenolic contents, antitumor effect and antioxidant capacity

Year 2022, Volume: 26 Issue: 4, 770 - 780, 28.06.2025

Shreen Nusair Mohammad Ahmad Ahmad Tamam El-elimat Khansaa Al-essa Luay Abu-qatouseh Rawand Khasawneh

Abstract

Natural products are potential sources of prospective antitumor agents. The phenolic and flavonoid
contents of leaves, flowers, and roots of Eryngium creticum were quantified using liquid chromatography-tandem mass
spectrometry (LC-ESI-MS/MS). The antioxidant capacity and antitumor activity of the three extracts were tested on
MCF-7 and Hep2. The average total phenolic contents of E. creticum leaves, flowers, and roots were 12.43 ± 0.24, 10.09 ±
0.20, and 4.78 ± 0.10 mg of GAE/g of dw, respectively. The average total flavonoids were 4.77 ± 0.10, 6.23 ± 0.12, and
5.62 ± 0.12 mg of QE/g of dw, respectively. The extracts showed considerable DPPH˙ scavenger activity with average
IC50 values of 1.41 ± 0.06, 2.70 ± 0.06, and 3.03 ± 0.05 µg/mL, respectively. The average IC50 values of scavenging capacity
of NO˙ radicals were 0.11 ± 0.03, 0.69 ± 0.04, and 0.44 ± 0.01 µg/mL, respectively. The average IC50 values in MCF-7 were
1.25 ± 0.50, 3.75 ± 0.48, and 1.88 ± 0.28 mg/mL, respectively. In Hep2, the average IC50 values were 1.16 ± 0.58, 3.75 ±
0.79, and 1.31 ± 0.47 µg/mL, respectively. Phenolic content varied between the different extracts; leaves showed the
highest levels of gallic acid (3.60 µg/mL), querciti-3-O-galactoside (0.82 µg/mL), cyanin chloride (1.52 µg/mL), and
rosmarinic acid (4.65 µg/mL). Roots showed the lowest quantities of the measured phenolics, except for chlorogenic
acid (6.00 µg/mL) and rosmarinic acid (1.86 µg/mL). The three extracts of E. creticum possess in vitro antitumor
activities, which could be utilized as an adjunct with chemotherapy.

Keywords

: Eryngium plant extract phenolic compounds cytotoxicity breast cancer antitumor agents

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There are 33 citations in total.

Details

Primary Language English
Subjects Clinical Pharmacy and Pharmacy Practice
Journal Section Articles
Authors

Shreen Nusair 0000-0001-7209-6249

Mohammad Ahmad Ahmad 0000-0002-3821-7606

Tamam El-elimat 0000-0002-9246-4974

Khansaa Al-essa 0000-0001-7688-5480

Luay Abu-qatouseh 0000-0003-1551-2343

Rawand Khasawneh 0000-0003-4583-2727

Publication Date June 28, 2025
Published in Issue Year 2022 Volume: 26 Issue: 4

Cite

APA Nusair, S., Ahmad, M. A., El-elimat, T., Al-essa, K., et al. (2025). Methanolic extract of Eryngium creticum Lam leaves, flowers and roots: Quantification and qualification of phenolic contents, antitumor effect and antioxidant capacity. Journal of Research in Pharmacy, 26(4), 770-780.
AMA Nusair S, Ahmad MA, El-elimat T, Al-essa K, Abu-qatouseh L, Khasawneh R. Methanolic extract of Eryngium creticum Lam leaves, flowers and roots: Quantification and qualification of phenolic contents, antitumor effect and antioxidant capacity. J. Res. Pharm. June 2025;26(4):770-780.
Chicago Nusair, Shreen, Mohammad Ahmad Ahmad, Tamam El-elimat, Khansaa Al-essa, Luay Abu-qatouseh, and Rawand Khasawneh. “Methanolic Extract of Eryngium Creticum Lam Leaves, Flowers and Roots: Quantification and Qualification of Phenolic Contents, Antitumor Effect and Antioxidant Capacity”. Journal of Research in Pharmacy 26, no. 4 (June 2025): 770-80.
EndNote Nusair S, Ahmad MA, El-elimat T, Al-essa K, Abu-qatouseh L, Khasawneh R (June 1, 2025) Methanolic extract of Eryngium creticum Lam leaves, flowers and roots: Quantification and qualification of phenolic contents, antitumor effect and antioxidant capacity. Journal of Research in Pharmacy 26 4 770–780.
IEEE S. Nusair, M. A. Ahmad, T. El-elimat, K. Al-essa, L. Abu-qatouseh, and R. Khasawneh, “Methanolic extract of Eryngium creticum Lam leaves, flowers and roots: Quantification and qualification of phenolic contents, antitumor effect and antioxidant capacity”, J. Res. Pharm., vol. 26, no. 4, pp. 770–780, 2025.
ISNAD Nusair, Shreen et al. “Methanolic Extract of Eryngium Creticum Lam Leaves, Flowers and Roots: Quantification and Qualification of Phenolic Contents, Antitumor Effect and Antioxidant Capacity”. Journal of Research in Pharmacy 26/4 (June 2025), 770-780.
JAMA Nusair S, Ahmad MA, El-elimat T, Al-essa K, Abu-qatouseh L, Khasawneh R. Methanolic extract of Eryngium creticum Lam leaves, flowers and roots: Quantification and qualification of phenolic contents, antitumor effect and antioxidant capacity. J. Res. Pharm. 2025;26:770–780.
MLA Nusair, Shreen et al. “Methanolic Extract of Eryngium Creticum Lam Leaves, Flowers and Roots: Quantification and Qualification of Phenolic Contents, Antitumor Effect and Antioxidant Capacity”. Journal of Research in Pharmacy, vol. 26, no. 4, 2025, pp. 770-8.
Vancouver Nusair S, Ahmad MA, El-elimat T, Al-essa K, Abu-qatouseh L, Khasawneh R. Methanolic extract of Eryngium creticum Lam leaves, flowers and roots: Quantification and qualification of phenolic contents, antitumor effect and antioxidant capacity. J. Res. Pharm. 2025;26(4):770-8.