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Ameliorative effect of yam bean (Pachyrhizus erosus L.) fiber on glucagon-like peptide 1 expression, oxidative stress and histopathology of the small ıntestine in mice fed high-fat diet

Year 2024, Volume: 28 Issue: 5, 1400 - 1408, 28.06.2025

Putra Santoso , Wilka Ramadhia Resti Rahayu Rita Maliza

Abstract

Yam bean (Pachyrhizus erosus L.) is a prominent medicinal plant exerting various health benefits. However, it remains unknown whether yam bean fiber (YB) is also capable of counteracting the deleterious effects of a high-fat diet (HFD) on the small intestine. This present study aimed to investigate the effect of YB supplementation on the glucagon-like peptide 1 (GLP-1) expression, oxidative stress, and histopathological alterations of the small intestine in mice fed an HFD. Twenty-seven mice were assigned equally into three different diet treatment groups: normal diet (ND), HFD, and HFD supplemented with 10% of YB (HFD + YB). After eight weeks, mRNA expression of GLP-1, malondialdehyde (MDA) levels, catalase (CAT) activity in the small intestinal tissue were determined, and the anatomical and histopathological alterations of the intestine were investigated. The results demonstrated that YB supplementation was effective in precluding HFD-indued reduction of GLP-1 mRNA expression in the small intestine. YBF also prevented MDA elevation while ameliorating CAT activity. Furthermore, YBF exerted an ameliorative effect against HFD-induced morphological and histological alterations of the small intestine, particularly in intestinal length, index, and the number of inflammatory cells in the mucosal layer. This study revealed that YB supplementation could effectively prevent the impairment of GLP-1 expression and oxidative stress while ameliorating the histopathological alterations of the small intestine caused by HFD. Hence, YB could be formulated as a supplement to improve the gastrointestinal structure and functions against HFD.

Keywords

catalase activity GLP-1 inflammatory cells malondialdehyde oxidative stress small intestine yam bean

References

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There are 35 citations in total.

Details

Primary Language English
Subjects Pharmacology and Pharmaceutical Sciences (Other)
Journal Section Articles
Authors

Putra Santoso 0000-0003-4092-6222

Wilka Ramadhia 0009-0003-0250-988X

Resti Rahayu 0000-0002-8882-0593

Rita Maliza 0000-0002-8494-314X

Publication Date June 28, 2025
Published in Issue Year 2024 Volume: 28 Issue: 5

Cite

APA Santoso, P., Ramadhia, W., Rahayu, R., Maliza, R. (2025). Ameliorative effect of yam bean (Pachyrhizus erosus L.) fiber on glucagon-like peptide 1 expression, oxidative stress and histopathology of the small ıntestine in mice fed high-fat diet. Journal of Research in Pharmacy, 28(5), 1400-1408.
AMA Santoso P, Ramadhia W, Rahayu R, Maliza R. Ameliorative effect of yam bean (Pachyrhizus erosus L.) fiber on glucagon-like peptide 1 expression, oxidative stress and histopathology of the small ıntestine in mice fed high-fat diet. J. Res. Pharm. July 2025;28(5):1400-1408.
Chicago Santoso, Putra, Wilka Ramadhia, Resti Rahayu, and Rita Maliza. “Ameliorative Effect of Yam Bean (Pachyrhizus Erosus L.) Fiber on Glucagon-Like Peptide 1 Expression, Oxidative Stress and Histopathology of the Small ıntestine in Mice Fed High-Fat Diet”. Journal of Research in Pharmacy 28, no. 5 (July 2025): 1400-1408.
EndNote Santoso P, Ramadhia W, Rahayu R, Maliza R (July 1, 2025) Ameliorative effect of yam bean (Pachyrhizus erosus L.) fiber on glucagon-like peptide 1 expression, oxidative stress and histopathology of the small ıntestine in mice fed high-fat diet. Journal of Research in Pharmacy 28 5 1400–1408.
IEEE P. Santoso, W. Ramadhia, R. Rahayu, and R. Maliza, “Ameliorative effect of yam bean (Pachyrhizus erosus L.) fiber on glucagon-like peptide 1 expression, oxidative stress and histopathology of the small ıntestine in mice fed high-fat diet”, J. Res. Pharm., vol. 28, no. 5, pp. 1400–1408, 2025.
ISNAD Santoso, Putra et al. “Ameliorative Effect of Yam Bean (Pachyrhizus Erosus L.) Fiber on Glucagon-Like Peptide 1 Expression, Oxidative Stress and Histopathology of the Small ıntestine in Mice Fed High-Fat Diet”. Journal of Research in Pharmacy 28/5 (July 2025), 1400-1408.
JAMA Santoso P, Ramadhia W, Rahayu R, Maliza R. Ameliorative effect of yam bean (Pachyrhizus erosus L.) fiber on glucagon-like peptide 1 expression, oxidative stress and histopathology of the small ıntestine in mice fed high-fat diet. J. Res. Pharm. 2025;28:1400–1408.
MLA Santoso, Putra et al. “Ameliorative Effect of Yam Bean (Pachyrhizus Erosus L.) Fiber on Glucagon-Like Peptide 1 Expression, Oxidative Stress and Histopathology of the Small ıntestine in Mice Fed High-Fat Diet”. Journal of Research in Pharmacy, vol. 28, no. 5, 2025, pp. 1400-8.
Vancouver Santoso P, Ramadhia W, Rahayu R, Maliza R. Ameliorative effect of yam bean (Pachyrhizus erosus L.) fiber on glucagon-like peptide 1 expression, oxidative stress and histopathology of the small ıntestine in mice fed high-fat diet. J. Res. Pharm. 2025;28(5):1400-8.