Vildagliptin loaded polymeric nanoparticles using cross-linked polymer by ionotropic gelation method: development and characterization
Abstract
The aim of present work is to formulate and characterize the Vildagliptin (VLD) loaded polymeric nanoparticles for the treatment of type II diabetes mellitus. Polymeric nanoparticles were prepared by ionotropic gelation method with sodium tripolyphosphate (STPP) as a cross linkers. The obtained sample of VLD was evaluated by UV and FT-IR studies. Prepared polymeric nanoparticles were characterized in term of drug-polymer intraction, particle size and polydispersity index (PDI), zeta potential, % drug content (DC), % drug entrapment efficiency (DEE) and scanning electron microscopy (SEM). In vitro and in vivo drug release studies were also performed. FT-IR study showed that drug and polymers are compatible with each other and cross-linking was observed between chitosan and STPP. Particle size was range from 238.2 nm to 708.8nm for all the formulations. Zeta potential was range from-8.49mV to 11.1mV. %DC range from68.12% to 84.37%, and % DEE was range from 78.76% to 93.91%. SEM images showed that the prepared formulations were smooth and spherical. In vitro drug release evaluation in phosphate buffer pH 7.4 showed sustained release pattern, i.e. 55.17% to 62.89% within 12hours. The data were fitted to Korsmeyer-Peppas model which showed fickian diffusion as a predominant release mechanism. In vivo drug release evalutionshowed significant decrease in the blood glucose level. Thus, conclude that prepared polymeric nanoparticles by ionotropic gelation method have a sustain release pattern and incresesstabilty of drug.
Keywords
Vildagliptin Chitosan STPP Ionotropic gelation method Polymeric nanoparticle anti diabetic activity
References
- [1] Pal SL, Jana U, Manna PK, Mohanta GP, Manavalan R. Nanoparticle: An overview of preparation and characterization. J Appl Pharm Sci 2011; 30: 228-234.
- [2] Yadav N, Khatak S, Sara US. Solid lipid nanoparticles-a review.Int J Appl Pharm 2013; 5(2): 8-18.
- [3] Mohanraj VJ, Chen Y. Nanoparticles-a review. Trop J Pharm Res. 2006; 5(1): 561-573. https://doi.org/10.4314/tjpr.v5i1.14634
- [4] Saharan P, Bhatt DC, Saharan SP, Bahmani K. Preparation and characterization of antidiabetic drug loaded polymeric nanoparticles. Der PharmaChemica. 2015; 7(12): 398-404.
- [5] Wild S, Roglic G, Green A, Sicree R, King H. Global prevalence of diabetes: estimates for the year 2000 and projections for 2030. Diabetes care. 2004; 27(5): 1047-1053. https://doi.org/10.2337/diacare.27.5.1047
- [6] Kwon YI, Vattem DA, Shetty K. Evaluation of clonal herbs of Lamiaceae species for management of diabetes and hypertension.Asia Pac J ClinNutr.2006; 15(1): 107-117.
- [7] Chandramore K. Review on Dipeptidyl Peptidase IV Inhibitors as a Newer Target for Diabetes Mellitus Treatment. Asian J Pharma Res. 2017; 7(4): 230-238.
- [8] Keating GM. Vildagliptin: a review of its use in type 2 diabetes mellitus. Adıs drug eval. 2014; 74(5): 587-610. https://doi.org/10.1007/s40265-014-0199-3
- [9] Naveed S, Rehman H, Qamar F, Zainab S. Method development and validation of Vildagliptin using UV spectrophotometer. Int J Pharm Sci Res. 2014; 5(10): 714-717.
- [10] Bhokare SG, Marathe RP. Rosuvastatin calcium loaded chitosan nanoparticles: preparation evaluation and in vitro release studies. Int J App Pharm. 2020; 12(5): 95-102. https://doi.org/10.22159/ijap.2020v12i5.38175
- [11] Berthomieu C, Hienerwade R. Fourier transform infrared (FTIR) spectroscopy. Photosynth Res 2009; 101: 157–170. https://doi.org/10.1007/s11120-009-9439-x
- [12] Raffin RP, Jornada DS, Re MI, Pohlmann AR, Guterres SS. Sodium pantoprazole-loaded enteric microparticles prepared by spray drying: Effect of the scale of production and process validation. Int J pharma. 2006; 324(1): 10-8.https://doi.org/10.1016/j.ijpharm.2006.06.045
- [13] Lason E, Sikora E Ogonowski J. Influence of process parameters on properties of Nanostructured Lipid Carriers (NLC) formulation. ActaBiochimicaPolonica. 2013; 60(4): 773-777. https://doi.org/10.3389/abp.2024.12821
- [14] Zaka F. Scanning Electron Microscopy Procedure for HE Powders on a LEO 438VP System. Lawrence Livermore National Lab, Livermore 2016: 1-8. https://doi.org/10.2172/1259748
- [15] Bindu HS, Bhavya G, Padmalatha K. Fomulation and in vitro evaluation of Vildagliptin Floating tablets. Ind Am J Pharm Sci. 2021; 8(07): 136-145.
- [16] Nagaraja SH, Al-Dhubiab BE, Tekade RK, Venugopala KN, Ghorpade RV, Meravanige G. Novel preparation and effective delivery of mucoadeshive nanoparticles containing anti-diabetic drug. Ind J Pharm Edu Res. 2019; 53(2):43- 49. https://doi.org10.5530/ijper.53.2s.47 [17] Costa P, Lobo JM. Modeling and comparison of dissolution profiles.Eur J pharm sci. 2001; 13(2): 123-133. https://doi.org/10.1016/S0928-0987(01)00095-1
- [18] Hasan MS, Uddin MG, Shoibe M, Al Mahmud A, Banik S. Evaluation of anxiolytic and hypoglycemic potential of CissusadnataRoxb. in animal model. J Complementary Integrative Med. 2020; 17(2): 1-7. https://doi.org/10.1515/jcim-2018-0130
- [19] Aslan M, Orhan N, Orhan DD, Ergun F. Hypoglycemic activity and antioxidant potential of some medicinal plants traditionally used in Turkey for diabetes. J Ethnopharmacology. 2010; 128(2): 384-389. https://doi.org10.1016/j.jep.2010.01.040
- [20] Waghulde M, Rajput R, Mujumdar A, Naik J. Production and evaluation of vildagliptin-loaded poly (dl-lactide) and poly (dl-lactide-glycolide) micro-/nanoparticles: Response surface methodology approach. Drying Technology. 2018 1-12. https://doi.org10.1080/07373937.2018.1495231
Details
| Primary Language | English |
|---|---|
| Subjects | Pharmaceutical Delivery Technologies |
| Journal Section | Articles |
| Authors | |
| Publication Date | June 28, 2025 |
| Published in Issue | Year 2024 Volume: 28 Issue: 5 |