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Bioassay-guided isolation of constituents from the endophytic fungi Fusarium solani inhabiting Rhizophora apiculata Blume

Year 2024, Volume: 28 Issue: 5, 1458 - 1464, 28.06.2025

Mario A. Tan John Vincent G. Gonzales Melfei E. Bungihan

Abstract

Endophytic fungi (EF) are excellent sources of diverse and pharmacologically relevant molecules for drug development. The mangrove genus Rhizophora have been identified to contain fungal isolates. In this study, the EF Fusarium solani was isolated and characterized from R. apiculata. Disk diffusion assay of the crude ethyl acetate extract of F. solani on the ESKAPE clinical isolate pathogens showed inhibitory activities against Escherichia coli 5218S (ZOI 12.20 mm), methicillin-resistant Staphylococcus aureus 2729B (ZOI 8.90 mm), Klebsiella pneumoniae 4830WD (ZOI 12.58 mm), Acinetobacter baumannii 3975B (ZOI 12.85 mm), and Enterobacter cloacae 5276S (ZOI 15.53 mm). Bioassay guided isolation and spectroscopic analysis led to the identification of benzoic acid (1) and bis(3-methylhexyl) phthalate (2). Microtiter plate antimicrobial assay gave minimum inhibitory concentration (MIC) of 125 g/mL for compound 1 and 250 g/mL for compound 2. To the best of our knowledge, this is the first report on the isolation of F. solani from R. apiculata and the isolation of compounds 1 and 2 from the genus Fusarium. Hence, this study validated the importance of the EF as sources of bioactive molecules.

Keywords

Rhizophora Fusarium solani Benzoic acid ESKAPE Enterobacter cloacae

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There are 25 citations in total.

Details

Primary Language English
Subjects Pharmaceutical Chemistry
Journal Section Articles
Authors

Mario A. Tan 0000-0002-0422-8120

John Vincent G. Gonzales 0009-0006-4226-9456

Melfei E. Bungihan 0000-0002-8122-8087

Publication Date June 28, 2025
Submission Date November 16, 2023
Acceptance Date December 24, 2023
Published in Issue Year 2024 Volume: 28 Issue: 5

Cite

APA Tan, M. A., Gonzales, J. V. G., & Bungihan, M. E. (2025). Bioassay-guided isolation of constituents from the endophytic fungi Fusarium solani inhabiting Rhizophora apiculata Blume. Journal of Research in Pharmacy, 28(5), 1458-1464.
AMA Tan MA, Gonzales JVG, Bungihan ME. Bioassay-guided isolation of constituents from the endophytic fungi Fusarium solani inhabiting Rhizophora apiculata Blume. J. Res. Pharm. July 2025;28(5):1458-1464.
Chicago Tan, Mario A., John Vincent G. Gonzales, and Melfei E. Bungihan. “Bioassay-Guided Isolation of Constituents from the Endophytic Fungi Fusarium Solani Inhabiting Rhizophora Apiculata Blume”. Journal of Research in Pharmacy 28, no. 5 (July 2025): 1458-64.
EndNote Tan MA, Gonzales JVG, Bungihan ME (July 1, 2025) Bioassay-guided isolation of constituents from the endophytic fungi Fusarium solani inhabiting Rhizophora apiculata Blume. Journal of Research in Pharmacy 28 5 1458–1464.
IEEE M. A. Tan, J. V. G. Gonzales, and M. E. Bungihan, “Bioassay-guided isolation of constituents from the endophytic fungi Fusarium solani inhabiting Rhizophora apiculata Blume”, J. Res. Pharm., vol. 28, no. 5, pp. 1458–1464, 2025.
ISNAD Tan, Mario A. et al. “Bioassay-Guided Isolation of Constituents from the Endophytic Fungi Fusarium Solani Inhabiting Rhizophora Apiculata Blume”. Journal of Research in Pharmacy 28/5 (July 2025), 1458-1464.
JAMA Tan MA, Gonzales JVG, Bungihan ME. Bioassay-guided isolation of constituents from the endophytic fungi Fusarium solani inhabiting Rhizophora apiculata Blume. J. Res. Pharm. 2025;28:1458–1464.
MLA Tan, Mario A. et al. “Bioassay-Guided Isolation of Constituents from the Endophytic Fungi Fusarium Solani Inhabiting Rhizophora Apiculata Blume”. Journal of Research in Pharmacy, vol. 28, no. 5, 2025, pp. 1458-64.
Vancouver Tan MA, Gonzales JVG, Bungihan ME. Bioassay-guided isolation of constituents from the endophytic fungi Fusarium solani inhabiting Rhizophora apiculata Blume. J. Res. Pharm. 2025;28(5):1458-64.