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Development and validation of an RP-HPLC method to compare the apoptotic activity of quercetin found in marketed dietary supplements

Year 2024, Volume: 28 Issue: 5, 1653 - 1664, 28.06.2025

Ümit Can Erim , Nese Ayşit

Abstract

Quercetin is a ubiquitous bioactive flavonoid found in broad selection of dietary supplements. It has potential beneficial health effects and induces therapeutic activity in many diseases including different types of cancer. Quercetin exerts cytotoxic activity in cancer and induces apoptotic cell death. Albeit, each dietary supplement consists of impurities, which in turn diminish the biological activity of the quercetin. Herein, we extracted quercetin from 5 different marketed dietary supplements in tablet formulation and detected their quercetin content by developing and validating an RP-HPLC method. We further investigated the cytotoxic activity of quercetin and its role in apoptosis through mitochondrial pathway in MCF-7 and 4T1 breast cancer cell lines. Our findings demonstrated that the quercetin content in all formulations was less than the amount stated in the labeling information, nevertheless they exerted significant cytotoxic activities in breast cancer cells. We revealed that formulations with high quercetin content induced apoptosis through restoring the tumor suppression activity of p53 and activating downstream caspases, whereas the formulation with low quercetin content potentially induced non-apoptotic cell death. Our work illustrates the involvement of quercetin in apop

Keywords

RP-HPLC Quercetin supplement method validation flavonoids apoptosis breast cancer

References

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There are 31 citations in total.

Details

Primary Language English
Subjects Pharmaceutical Analytical Chemistry
Journal Section Articles
Authors

Ümit Can Erim 0000-0002-4121-588X

Nese Ayşit 0000-0002-4243-9092

Publication Date June 28, 2025
Submission Date October 25, 2023
Acceptance Date February 28, 2024
Published in Issue Year 2024 Volume: 28 Issue: 5

Cite

APA Erim, Ü. C., & Ayşit, N. (2025). Development and validation of an RP-HPLC method to compare the apoptotic activity of quercetin found in marketed dietary supplements. Journal of Research in Pharmacy, 28(5), 1653-1664.
AMA Erim ÜC, Ayşit N. Development and validation of an RP-HPLC method to compare the apoptotic activity of quercetin found in marketed dietary supplements. J. Res. Pharm. July 2025;28(5):1653-1664.
Chicago Erim, Ümit Can, and Nese Ayşit. “Development and Validation of an RP-HPLC Method to Compare the Apoptotic Activity of Quercetin Found in Marketed Dietary Supplements”. Journal of Research in Pharmacy 28, no. 5 (July 2025): 1653-64.
EndNote Erim ÜC, Ayşit N (July 1, 2025) Development and validation of an RP-HPLC method to compare the apoptotic activity of quercetin found in marketed dietary supplements. Journal of Research in Pharmacy 28 5 1653–1664.
IEEE Ü. C. Erim and N. Ayşit, “Development and validation of an RP-HPLC method to compare the apoptotic activity of quercetin found in marketed dietary supplements”, J. Res. Pharm., vol. 28, no. 5, pp. 1653–1664, 2025.
ISNAD Erim, Ümit Can - Ayşit, Nese. “Development and Validation of an RP-HPLC Method to Compare the Apoptotic Activity of Quercetin Found in Marketed Dietary Supplements”. Journal of Research in Pharmacy 28/5 (July 2025), 1653-1664.
JAMA Erim ÜC, Ayşit N. Development and validation of an RP-HPLC method to compare the apoptotic activity of quercetin found in marketed dietary supplements. J. Res. Pharm. 2025;28:1653–1664.
MLA Erim, Ümit Can and Nese Ayşit. “Development and Validation of an RP-HPLC Method to Compare the Apoptotic Activity of Quercetin Found in Marketed Dietary Supplements”. Journal of Research in Pharmacy, vol. 28, no. 5, 2025, pp. 1653-64.
Vancouver Erim ÜC, Ayşit N. Development and validation of an RP-HPLC method to compare the apoptotic activity of quercetin found in marketed dietary supplements. J. Res. Pharm. 2025;28(5):1653-64.