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Colorimetric analysis of phenols and flavanoids in pomegranate plant extract

Year 2024, Volume: 28 Issue: 5, 1720 - 1741, 28.06.2025

Sujiya Balla Nagasen Dasari Sharon Priyanka Palina Sathis Kumar Dinakaran Lavanya Pulidindi

Abstract

Punica granatum (Pomegranate) is widely accessible and used by natural health professionals for a range of medicinal purposes. The purpose of the current study to use a derivatization method in conjunction with FT-IR, colorimetry, and UV-Visible spectroscopy to determine the total phenolic and flavonoid content of various pomegranate sections. Pomegranate is used to treat ulcers, diarrhoea, Male infertility and so on. Currently, pomegranate is marketed in different novel drug formulations such as nanoparticles for local mucosal drug delivery, niosomes, hydrogels for chronic ınflammation, microemulsion for skin care and analytical techniques using HPLC, GC and GC-MS, LC-MS for carrying out the various quantitative and qualitative analyses are some examples of these developments. In this study, a part of pomegranate was extracted and evaluated by using Microscopical and Macroscopical evaluation, Fluorescence Analysis, FT-IR studies using derivatisation method and finally quantified the phenol and flavonoid content using UV Visible Spectroscopy. Results of the study revealed that the pomegranate extract gives data about the linearity, precision, accuracy of peel and bark ethanolic and aqueous extract gives linearity 1-5µg/ml, Regression 0.998, LOD 0.691µg/ml, LOQ 1.32µg/ml. Through the appropriate selection of Solvents, we successfully developed a stable and effective method for the determination and estimation of phenol and flavonoid content by derivatization and FTIR studies. Method was developed, optimized and validated with its parameters.

Keywords

Derivatization method Flavonoid content Phenolic content UV-Visible spectroscopy FT-IR Punica granatum (Pomegranate)

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There are 40 citations in total.

Details

Primary Language English
Subjects Pharmacognosy
Journal Section Articles
Authors

Sujiya Balla 0009-0009-5181-7313

Nagasen Dasari 0000-0002-8305-7277

Sharon Priyanka Palina 0009-0008-0692-1990

Sathis Kumar Dinakaran 0000-0002-4163-4785

Lavanya Pulidindi 0009-0004-4731-8419

Publication Date June 28, 2025
Submission Date October 22, 2023
Acceptance Date January 18, 2024
Published in Issue Year 2024 Volume: 28 Issue: 5

Cite

APA Balla, S., Dasari, N., Palina, S. P., Dinakaran, S. K., et al. (2025). Colorimetric analysis of phenols and flavanoids in pomegranate plant extract. Journal of Research in Pharmacy, 28(5), 1720-1741.
AMA Balla S, Dasari N, Palina SP, Dinakaran SK, Pulidindi L. Colorimetric analysis of phenols and flavanoids in pomegranate plant extract. J. Res. Pharm. July 2025;28(5):1720-1741.
Chicago Balla, Sujiya, Nagasen Dasari, Sharon Priyanka Palina, Sathis Kumar Dinakaran, and Lavanya Pulidindi. “Colorimetric Analysis of Phenols and Flavanoids in Pomegranate Plant Extract”. Journal of Research in Pharmacy 28, no. 5 (July 2025): 1720-41.
EndNote Balla S, Dasari N, Palina SP, Dinakaran SK, Pulidindi L (July 1, 2025) Colorimetric analysis of phenols and flavanoids in pomegranate plant extract. Journal of Research in Pharmacy 28 5 1720–1741.
IEEE S. Balla, N. Dasari, S. P. Palina, S. K. Dinakaran, and L. Pulidindi, “Colorimetric analysis of phenols and flavanoids in pomegranate plant extract”, J. Res. Pharm., vol. 28, no. 5, pp. 1720–1741, 2025.
ISNAD Balla, Sujiya et al. “Colorimetric Analysis of Phenols and Flavanoids in Pomegranate Plant Extract”. Journal of Research in Pharmacy 28/5 (July 2025), 1720-1741.
JAMA Balla S, Dasari N, Palina SP, Dinakaran SK, Pulidindi L. Colorimetric analysis of phenols and flavanoids in pomegranate plant extract. J. Res. Pharm. 2025;28:1720–1741.
MLA Balla, Sujiya et al. “Colorimetric Analysis of Phenols and Flavanoids in Pomegranate Plant Extract”. Journal of Research in Pharmacy, vol. 28, no. 5, 2025, pp. 1720-41.
Vancouver Balla S, Dasari N, Palina SP, Dinakaran SK, Pulidindi L. Colorimetric analysis of phenols and flavanoids in pomegranate plant extract. J. Res. Pharm. 2025;28(5):1720-41.