Aromatic Phosphinous Amides: A Promising New Generation of Antibiotics for Multidrug-Resistant Bacterial Infections
Abstract
There is a focus on using aromatic phosphinous amide as an antibacterial agent against serious multidrug resistant bacterial infections. N–(4–acetylphenyl)–P,P–diphenyl-phosphinous amide and its corresponding thioxo- phosphinous amide and selenoxo-phosphinous amide were synthesized and designated Ph1, Ph2, and Ph3, respectively. They were structurally identified by single crystal X-ray diffraction, multinuclear NMR spectroscopy, and elemental analysis. Because their antibacterial activities have never been examined, this study aimed to assess their antibacterial activities against both Gram-positive and Gram-negative bacteria as well as readily available standard bacterial strains. Two bacterial isolates were isolated from urine specimens collected from patients with urinary tract infections and designated CS1, and CS2. Phenotypic, biochemical, and molecular approaches were used to identify them. The antibiotic susceptibility/resistance pattern of these isolates was examined by the installed VITEK 2 system. Isolate CS1 is an aerobic Gram-positive, straight rod-shaped, spore forming, occurring singly or in a short chain, whereas isolate CS2 is an aerobic Gram-negative, coccobacilli-shaped, non-spore forming, occurring in pairs or in chains. Both isolates are positive for catalase and oxidase test. According to an examination of the 16S rRNA gene sequence, the isolates CS1 and CS2 have 98% of their similarities with Bacillus and Acinetobacter species, respectively. Isolates CS1 and CS2 are multidrug resistance species. Based on the results of the minimum inhibitory concentration, the standard bacterial strains and isolates CS1 and CS2 showed a wide range in the antibacterial capabilities of the studied phosphinous amides. In comparison to Ph2 and Ph3, Ph1 has the strongest antibacterial activity against all of the tested bacterial species. In conclusion, CS1 and CS2 isolates were identified as novel, multi-drug resistant members of the Bacillus and Acinetobacter genera, respectively. The Ph1 molecule represents a promising new generation of antibiotics with notable antibacterial efficacy against multidrug-resistant Gram-positive and Gram-negative microorganisms.
Keywords
Phosphinous amide derivatives Multidrug resistance bacteria 16S rRNA gene sequencing technique Structural analysis Minimum inhibitory concentration
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Details
| Primary Language | English |
|---|---|
| Subjects | Pharmacology and Pharmaceutical Sciences (Other) |
| Journal Section | Articles |
| Authors | |
| Publication Date | June 28, 2025 |
| Published in Issue | Year 2024 Volume: 28 Issue: 5 |