Evaluation of anti-diabetic potential of Ficus arnottiana Miq. leaves against in-vivo and in-vitro experimental models of diabetes

Shivakumar Inamdar; Pallavi S Anwar; Kaneez Fatima; Makhmur Ahmad; B Tazneem; Pankaj Yadav; Mohammad Sarafroz; Abdullah Khan; Mohamad Taleuzzaman

Research Article

Evaluation of anti-diabetic potential of Ficus arnottiana Miq. leaves against in-vivo and in-vitro experimental models of diabetes

Year 2024, Volume: 28 Issue: 5, 1844 - 1854, 28.06.2025

Shivakumar Inamdar Pallavi S Anwar Kaneez Fatima Makhmur Ahmad B Tazneem Pankaj Yadav Mohammad Sarafroz Abdullah Khan Mohamad Taleuzzaman

Abstract

The present study was aimed to investigate the potential anti-diabetic effects of Ficus arnottiana Miq. leaves against streptozotocin-induced diabetic model in experimental animals. The leaves of Ficus arnottiana were collected and processed into a powdered form, which was subsequently extracted using methanol as the solvent. The anti-diabetic activity of the resulting extract was evaluated at two oral doses (200 and 400 mg/kg) at two different doses (200 and 400 mg/kg). The parameters assessed in this study included blood glucose levels, C-peptide levels, and serum insulin levels. Furthermore, pancreatic tissue samples were subjected to histopathological analysis. Additionally, in vitro determination of -amylase and -glucosidase inhibition by the extract was carried out. The phytochemical screening of the extract revealed the presence of alkaloids, tannins, flavonoids, phenolic compounds, reducing sugars, and saponins. For anti-diabetic activity, the test extract significantly reduced blood glucose levels while increasing both serum C-peptide and serum insulin levels. Histopathological analysis of extract treated group depicted improved islets of Langerhans and an increase in centrally located beta cells. Furthermore, the extract significantly inhibited -amylase and - glucosidase enzyme in-vitro. Based on these findings, it can be concluded that Ficus arnottiana (Miq) leaves extract exhibits antidiabetic activity. And the anti-diabetic effect of Ficus arnottiana (Miq) at 400 mg/kg was comparable to that of the standard drugs Glibenclamide (in-vivo) and acarbose (in-vitro).

Keywords

Anti-diabetic activity, STZ induced diabetes, histopathological evaluation, α-amylase, α- glucosidase, Ficus arnottiana (Miq)

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There are 18 citations in total.

Details

Primary Language	English
Subjects	Pharmacology and Pharmaceutical Sciences (Other)
Journal Section	Articles
Authors	Shivakumar Inamdar 0000-0002-4172-4633 Pallavi S Anwar 0009-0008-7756-6674 Kaneez Fatima 0000-0002-0188-0834 Makhmur Ahmad 0000-0003-4550-5611 B Tazneem 0000-0003-3220-2988 Pankaj Yadav 0000-0002-6524-8803 Mohammad Sarafroz 0000-0002-3426-8238 Abdullah Khan 0000-0002-3437-8189 Mohamad Taleuzzaman 0000-0002-5201-3503
Publication Date	June 28, 2025
Published in Issue	Year 2024 Volume: 28 Issue: 5

Cite

APA	Inamdar, S., Anwar, P. S., Fatima, K., Ahmad, M., et al. (2025). Evaluation of anti-diabetic potential of Ficus arnottiana Miq. leaves against in-vivo and in-vitro experimental models of diabetes. Journal of Research in Pharmacy, 28(5), 1844-1854.
AMA	Inamdar S, Anwar PS, Fatima K, Ahmad M, Tazneem B, Yadav P, Sarafroz M, Khan A, Taleuzzaman M. Evaluation of anti-diabetic potential of Ficus arnottiana Miq. leaves against in-vivo and in-vitro experimental models of diabetes. J. Res. Pharm. July 2025;28(5):1844-1854.
Chicago	Inamdar, Shivakumar, Pallavi S Anwar, Kaneez Fatima, Makhmur Ahmad, B Tazneem, Pankaj Yadav, Mohammad Sarafroz, Abdullah Khan, and Mohamad Taleuzzaman. “Evaluation of Anti-Diabetic Potential of Ficus Arnottiana Miq. Leaves Against in-Vivo and in-Vitro Experimental Models of Diabetes”. Journal of Research in Pharmacy 28, no. 5 (July 2025): 1844-54.
EndNote	Inamdar S, Anwar PS, Fatima K, Ahmad M, Tazneem B, Yadav P, Sarafroz M, Khan A, Taleuzzaman M (July 1, 2025) Evaluation of anti-diabetic potential of Ficus arnottiana Miq. leaves against in-vivo and in-vitro experimental models of diabetes. Journal of Research in Pharmacy 28 5 1844–1854.
IEEE	S. Inamdar, P. S. Anwar, K. Fatima, M. Ahmad, B. Tazneem, P. Yadav, M. Sarafroz, A. Khan, and M. Taleuzzaman, “Evaluation of anti-diabetic potential of Ficus arnottiana Miq. leaves against in-vivo and in-vitro experimental models of diabetes”, J. Res. Pharm., vol. 28, no. 5, pp. 1844–1854, 2025.
ISNAD	Inamdar, Shivakumar et al. “Evaluation of Anti-Diabetic Potential of Ficus Arnottiana Miq. Leaves Against in-Vivo and in-Vitro Experimental Models of Diabetes”. Journal of Research in Pharmacy 28/5 (July 2025), 1844-1854.
JAMA	Inamdar S, Anwar PS, Fatima K, Ahmad M, Tazneem B, Yadav P, Sarafroz M, Khan A, Taleuzzaman M. Evaluation of anti-diabetic potential of Ficus arnottiana Miq. leaves against in-vivo and in-vitro experimental models of diabetes. J. Res. Pharm. 2025;28:1844–1854.
MLA	Inamdar, Shivakumar et al. “Evaluation of Anti-Diabetic Potential of Ficus Arnottiana Miq. Leaves Against in-Vivo and in-Vitro Experimental Models of Diabetes”. Journal of Research in Pharmacy, vol. 28, no. 5, 2025, pp. 1844-5.
Vancouver	Inamdar S, Anwar PS, Fatima K, Ahmad M, Tazneem B, Yadav P, Sarafroz M, Khan A, Taleuzzaman M. Evaluation of anti-diabetic potential of Ficus arnottiana Miq. leaves against in-vivo and in-vitro experimental models of diabetes. J. Res. Pharm. 2025;28(5):1844-5.