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Protection of prefrontal cortex neurons and improvement of memory in epileptic rats by 1-triacontanol cerotate

Year 2024, Volume: 28 Issue: 5, 1572 - 1580, 28.06.2025

Snehunsu Adhikari Sayam Subhash Satheesha Nayak Christofer Thomas Chinmay Suryavanshi

Abstract

Epilepsy treatment continues to face significant challenges, including drug resistance and cognitive impairment associated with antiepileptic drugs. 1-Triacontanol cerotate (1TAC), an active component isolated from Marsilea quadrifolia Linn., has emerged as a potential therapeutic avenue for epilepsy. This study investigated the effects of 1TAC on prefrontal cortical neurons and memory retention in chronically epileptic rats, comparing its efficacy to sodium valproate. Our experiment utilized two-month-old adult male Wistar rats, randomly assigned to one of five groups: I - Vehicle Control, II - Rats receiving Pentylenetetrazol (PTZ) at a dosage of 35 mg/kg body weight intraperitoneally every 48 hours, III - Rats given 200 mg/kg body weight of sodium valproate 30 minutes prior to PTZ administration, IV and V - Rats administered 40 and 80 mg/kg body weight of 1-TAC orally 30 minutes before PTZ challenge, respectively. To assess memory performance, we conducted a passive avoidance test. Subsequently, brain specimens were processed for cresyl violet staining to assess cell densities. Results demonstrated that epileptic rats treated with sodium valproate before PTZ administration, and those given 80 mg of 1-Triacontanol cerotate exhibited significantly enhanced memory retention compared to the untreated epileptic group at both 24- and 48-hours post-challenge. Furthermore, 80 mg of 1-Triacontanol cerotate administration showed a protective effect by significantly reducing the loss of pyramidal cells in the medial prefrontal cortex. This intervention effectively minimized the loss of pyramidal neurons in the medial prefrontal cortex and mitigated memory deficits in chronically epileptic rats.

Keywords

1-Triacontanol cerotate epilepsy Marsilea quadrifolia Linn memory prefrontal cortex

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There are 32 citations in total.

Details

Primary Language English
Subjects Medical Pharmacology
Journal Section Articles
Authors

Snehunsu Adhikari 0000-0003-3244-0505

Sayam Subhash 0009-0009-8639-7670

Satheesha Nayak 0000-0003-1421-8222

Christofer Thomas 0000-0002-8899-0496

Chinmay Suryavanshi 0000-0001-9365-2996

Publication Date June 28, 2025
Submission Date November 9, 2023
Acceptance Date January 29, 2024
Published in Issue Year 2024 Volume: 28 Issue: 5

Cite

APA Adhikari, S., Subhash, S., Nayak, S., Thomas, C., et al. (2025). Protection of prefrontal cortex neurons and improvement of memory in epileptic rats by 1-triacontanol cerotate. Journal of Research in Pharmacy, 28(5), 1572-1580.
AMA Adhikari S, Subhash S, Nayak S, Thomas C, Suryavanshi C. Protection of prefrontal cortex neurons and improvement of memory in epileptic rats by 1-triacontanol cerotate. J. Res. Pharm. July 2025;28(5):1572-1580.
Chicago Adhikari, Snehunsu, Sayam Subhash, Satheesha Nayak, Christofer Thomas, and Chinmay Suryavanshi. “Protection of Prefrontal Cortex Neurons and Improvement of Memory in Epileptic Rats by 1-Triacontanol Cerotate”. Journal of Research in Pharmacy 28, no. 5 (July 2025): 1572-80.
EndNote Adhikari S, Subhash S, Nayak S, Thomas C, Suryavanshi C (July 1, 2025) Protection of prefrontal cortex neurons and improvement of memory in epileptic rats by 1-triacontanol cerotate. Journal of Research in Pharmacy 28 5 1572–1580.
IEEE S. Adhikari, S. Subhash, S. Nayak, C. Thomas, and C. Suryavanshi, “Protection of prefrontal cortex neurons and improvement of memory in epileptic rats by 1-triacontanol cerotate”, J. Res. Pharm., vol. 28, no. 5, pp. 1572–1580, 2025.
ISNAD Adhikari, Snehunsu et al. “Protection of Prefrontal Cortex Neurons and Improvement of Memory in Epileptic Rats by 1-Triacontanol Cerotate”. Journal of Research in Pharmacy 28/5 (July 2025), 1572-1580.
JAMA Adhikari S, Subhash S, Nayak S, Thomas C, Suryavanshi C. Protection of prefrontal cortex neurons and improvement of memory in epileptic rats by 1-triacontanol cerotate. J. Res. Pharm. 2025;28:1572–1580.
MLA Adhikari, Snehunsu et al. “Protection of Prefrontal Cortex Neurons and Improvement of Memory in Epileptic Rats by 1-Triacontanol Cerotate”. Journal of Research in Pharmacy, vol. 28, no. 5, 2025, pp. 1572-80.
Vancouver Adhikari S, Subhash S, Nayak S, Thomas C, Suryavanshi C. Protection of prefrontal cortex neurons and improvement of memory in epileptic rats by 1-triacontanol cerotate. J. Res. Pharm. 2025;28(5):1572-80.