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Purification of acethylcholinesterase from the mollusc Mytilus galloprovincialis Lam. and investigation of its kinetic properties

Year 2019, Volume: 23 Issue: 4, 759 - 769, 27.06.2025

Servet Duranay Sezin Anıl , Gözde Hasbal Nurten Ozsoy

Abstract

The role of acetylcholinesterase in terminating acetylcholine-mediated neurotransmission made it the focus of intense research. In this study the haemolymph Acetylcholinesterase (AChE) from the mollusc Mytilus galloprovincialis was purified to homogeneity by (NH4)2SO4 fractional precipitation and affinity chromatography on edrophonium-Sepharose 6B. The enzyme was purified approximatedly 13 fold over the crude extract and was obtained in 3 % yield. The specific activity of purified enzyme was 3 U/mg protein. It had an optimum pH of 7.5 and showed optimal activity at 35oC. Km and Vmax for acetylthiocholine iodide were 1.3 mM and 0.188 mM/mg/min, respectively. The purified enzyme migrated as a 51 000 dalton band during polyacrylamide gel electrophoresis under denaturing and reducing conditions. Three methoxyflavones were examined in order to evaluate their potential as anti-Alzheimer’s Disease (AD) agents. All of the compounds were shown to be potent AChE inhibitors. Therefore, these compounds may have great value in the development of therapeutic and preventive agents for AD.

Keywords

Acethylcholinesterase Mytilus galloprovincialis Lam. enzyme purification kinetic properties inhibition by methoxyflavones.

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There are 44 citations in total.

Details

Primary Language English
Subjects Pharmaceutical Biochemistry, Pharmacognosy
Journal Section Articles
Authors

Servet Duranay

Sezin Anıl

Gözde Hasbal

Nurten Ozsoy

Publication Date June 27, 2025
Published in Issue Year 2019 Volume: 23 Issue: 4

Cite

APA Duranay, S., Anıl, S., Hasbal, G., Ozsoy, N. (2025). Purification of acethylcholinesterase from the mollusc Mytilus galloprovincialis Lam. and investigation of its kinetic properties. Journal of Research in Pharmacy, 23(4), 759-769.
AMA Duranay S, Anıl S, Hasbal G, Ozsoy N. Purification of acethylcholinesterase from the mollusc Mytilus galloprovincialis Lam. and investigation of its kinetic properties. J. Res. Pharm. June 2025;23(4):759-769.
Chicago Duranay, Servet, Sezin Anıl, Gözde Hasbal, and Nurten Ozsoy. “Purification of Acethylcholinesterase from the Mollusc Mytilus Galloprovincialis Lam. And Investigation of Its Kinetic Properties”. Journal of Research in Pharmacy 23, no. 4 (June 2025): 759-69.
EndNote Duranay S, Anıl S, Hasbal G, Ozsoy N (June 1, 2025) Purification of acethylcholinesterase from the mollusc Mytilus galloprovincialis Lam. and investigation of its kinetic properties. Journal of Research in Pharmacy 23 4 759–769.
IEEE S. Duranay, S. Anıl, G. Hasbal, and N. Ozsoy, “Purification of acethylcholinesterase from the mollusc Mytilus galloprovincialis Lam. and investigation of its kinetic properties”, J. Res. Pharm., vol. 23, no. 4, pp. 759–769, 2025.
ISNAD Duranay, Servet et al. “Purification of Acethylcholinesterase from the Mollusc Mytilus Galloprovincialis Lam. And Investigation of Its Kinetic Properties”. Journal of Research in Pharmacy 23/4 (June 2025), 759-769.
JAMA Duranay S, Anıl S, Hasbal G, Ozsoy N. Purification of acethylcholinesterase from the mollusc Mytilus galloprovincialis Lam. and investigation of its kinetic properties. J. Res. Pharm. 2025;23:759–769.
MLA Duranay, Servet et al. “Purification of Acethylcholinesterase from the Mollusc Mytilus Galloprovincialis Lam. And Investigation of Its Kinetic Properties”. Journal of Research in Pharmacy, vol. 23, no. 4, 2025, pp. 759-6.
Vancouver Duranay S, Anıl S, Hasbal G, Ozsoy N. Purification of acethylcholinesterase from the mollusc Mytilus galloprovincialis Lam. and investigation of its kinetic properties. J. Res. Pharm. 2025;23(4):759-6.