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Piroxicam induced alteration on membrane depolarization and caspase levels in PC3 prostate cancer cells

Year 2022, Volume: 26 Issue: 6, 1646 - 1655, 28.06.2025

Cemile Şahiner Kışla Filiz Bakar Ateş

Abstract

The relationship between cancer and inflammation has been known for years, and the interaction between prostate cancer and inflammation and inflammatory agents is still under investigation. Studies have shown that nonsteroidal anti-inflammatory drugs (NSAIDs), which are effective in the treatment of inflammation, can also be effective in some types of cancer. In this study, the efficacy of piroxicam on human prostate cancer cells was evaluated. Piroxicam showed a significant cytotoxic effect in PC-3 cells at a concentration of 50 µM and above at the 48th hour, and induced cell cycle arrest in the S phase. On the other hand, piroxicam was found to increase annexin V binding in PC3 cells, and the early apoptotic cell population was found to be significantly higher at 25 µM and higher concentrations (p<0.05). The apoptosis-inducing effect of piroxicam was also confirmed by high total caspase levels (p<0.0001). In addition, increased membrane depolarization was observed in PC3 cells treated with piroxicam. In conclusion, this study showed the cytotoxic and apoptotic effect of piroxicam in PC-3 cells, and the obtained findings shed light on further studies on the use of piroxicam as a promising compound in prostate cancer.

Keywords

piroxicam PC3 Prostate cancer Apoptosis Caspase Mitopotential

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There are 44 citations in total.

Details

Primary Language English
Subjects Pharmacology and Pharmaceutical Sciences (Other)
Journal Section Articles
Authors

Cemile Şahiner Kışla

Filiz Bakar Ateş

Publication Date June 28, 2025
Published in Issue Year 2022 Volume: 26 Issue: 6

Cite

APA Şahiner Kışla, C., & Bakar Ateş, F. (2025). Piroxicam induced alteration on membrane depolarization and caspase levels in PC3 prostate cancer cells. Journal of Research in Pharmacy, 26(6), 1646-1655.
AMA Şahiner Kışla C, Bakar Ateş F. Piroxicam induced alteration on membrane depolarization and caspase levels in PC3 prostate cancer cells. J. Res. Pharm. June 2025;26(6):1646-1655.
Chicago Şahiner Kışla, Cemile, and Filiz Bakar Ateş. “Piroxicam Induced Alteration on Membrane Depolarization and Caspase Levels in PC3 Prostate Cancer Cells”. Journal of Research in Pharmacy 26, no. 6 (June 2025): 1646-55.
EndNote Şahiner Kışla C, Bakar Ateş F (June 1, 2025) Piroxicam induced alteration on membrane depolarization and caspase levels in PC3 prostate cancer cells. Journal of Research in Pharmacy 26 6 1646–1655.
IEEE C. Şahiner Kışla and F. Bakar Ateş, “Piroxicam induced alteration on membrane depolarization and caspase levels in PC3 prostate cancer cells”, J. Res. Pharm., vol. 26, no. 6, pp. 1646–1655, 2025.
ISNAD Şahiner Kışla, Cemile - Bakar Ateş, Filiz. “Piroxicam Induced Alteration on Membrane Depolarization and Caspase Levels in PC3 Prostate Cancer Cells”. Journal of Research in Pharmacy 26/6 (June 2025), 1646-1655.
JAMA Şahiner Kışla C, Bakar Ateş F. Piroxicam induced alteration on membrane depolarization and caspase levels in PC3 prostate cancer cells. J. Res. Pharm. 2025;26:1646–1655.
MLA Şahiner Kışla, Cemile and Filiz Bakar Ateş. “Piroxicam Induced Alteration on Membrane Depolarization and Caspase Levels in PC3 Prostate Cancer Cells”. Journal of Research in Pharmacy, vol. 26, no. 6, 2025, pp. 1646-55.
Vancouver Şahiner Kışla C, Bakar Ateş F. Piroxicam induced alteration on membrane depolarization and caspase levels in PC3 prostate cancer cells. J. Res. Pharm. 2025;26(6):1646-55.