Research Article
Antibacterial and antibiofilm activity of Melaleuca alternifolia (tea tree) essential oil against colistin resistant Salmonella enterica serotypes isolated from poultry environmental specimens
Abstract
The development of increasing resistance to antibiotics such as colistin, problems arise in the treatment of
bacterial infections and make it necessary to search new alternative methods. For this purpose, plant-based approaches
are among the important research topics depending on their traditional uses. The aim of the present study was to
investigate the antibacterial and antibiofilm activity of tea tree oil purchased from a local market against a variety of 66
colistin resistant Salmonella enterica serotypes isolated from poultry farm environmental samples. Content analysis of
TTO was determined by gas chromatography/mass spectroscopy. The antibacterial activity was determined by broth
microdilution method, and antibiofilm activity was examined by crystal violet method. As a result, terpinen-4-ol was
found as major component of TTO with 35.9% ratio. The MIC values of TTO were differed between 6250-12500 µg/mL.
27 of 66 isolates formed biofilm and 25 of 27 isolates belonged to S. Infantis. The biofilm reduction of TTO at sub-
inhibitory concentration were found between 52-84.4%. Current study should be supported by future studies to
determine the effectiveness of TTO to be among the agents that can be used together with antimicrobials in the
attenuation of microorganisms.
Keywords
antibacterial biofilm poultry Salmonella enterica tea tree oil Melaleuca alternifolia antibiotic resistance colistin
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Details
| Primary Language | English |
|---|---|
| Subjects | Pharmaceutical Microbiology |
| Journal Section | Articles |
| Authors | |
| Publication Date | June 27, 2025 |
| Published in Issue | Year 2023 Volume: 27 Issue: 2 |