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A newly developed and fully validated HPLC method for simultenous determination of in-situ single pass intestinal permeability of domperidone, metoprolol tartarate and phenol red

Year 2023, Volume: 27 Issue: 3, 967 - 979, 28.06.2025

Saniye Özcan , Emre Şefik Çağlar , Mustafa Sinan Kaynak , Neslihan Üstündağ

Abstract

One of the most popular methods for figuring out a drug’s intestinal permeability is the intestinal perfusion (SPIP) approach. Metoprolol as tartrate and phenol red are frequently utilized as reference compounds in perfusion tests to assess the permeability coefficient of the study’s focus component, Domperidone maleate. Our study’s goal was to create and validate a reversed-phase liquid chromatographic technique for the simultaneous detection of Domperidone maleate, metoprolol, and phenol red for use in intestinal perfusion studies. The analysis was performed on a C18 column 150 × 1.5 mm, 5.0 μm using a mobile phase consisting of methanol: 20 mM potassium dihydrogen phosphate buffer 43:57, v/v; pH 3.1. The procedure was validated using the FDA’s standards for stability, linearity, precision, and accuracy. All calibration curves were linear r2> 0.99. The system suitability parameters of the method are the required limits all analytes. The in situ intestinal permeability experiments can easily be standardized with the help of this newly created and proven approach.

Keywords

Domperidone HPLC validation Metoprolol tartarate Phenol Red

References

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There are 35 citations in total.

Details

Primary Language English
Subjects Pharmaceutical Analytical Chemistry
Journal Section Articles
Authors

Saniye Özcan 0000-0002-5492-0457

Emre Şefik Çağlar 0000-0003-2010-4918

Mustafa Sinan Kaynak 0000-0003-2917-2407

Neslihan Üstündağ 0000-0002-3210-3747

Publication Date June 28, 2025
Published in Issue Year 2023 Volume: 27 Issue: 3

Cite

APA Özcan, S., Çağlar, E. Ş., Kaynak, M. S., Üstündağ, N. (2025). A newly developed and fully validated HPLC method for simultenous determination of in-situ single pass intestinal permeability of domperidone, metoprolol tartarate and phenol red. Journal of Research in Pharmacy, 27(3), 967-979.
AMA Özcan S, Çağlar EŞ, Kaynak MS, Üstündağ N. A newly developed and fully validated HPLC method for simultenous determination of in-situ single pass intestinal permeability of domperidone, metoprolol tartarate and phenol red. J. Res. Pharm. June 2025;27(3):967-979.
Chicago Özcan, Saniye, Emre Şefik Çağlar, Mustafa Sinan Kaynak, and Neslihan Üstündağ. “A Newly Developed and Fully Validated HPLC Method for Simultenous Determination of in-Situ Single Pass Intestinal Permeability of Domperidone, Metoprolol Tartarate and Phenol Red”. Journal of Research in Pharmacy 27, no. 3 (June 2025): 967-79.
EndNote Özcan S, Çağlar EŞ, Kaynak MS, Üstündağ N (June 1, 2025) A newly developed and fully validated HPLC method for simultenous determination of in-situ single pass intestinal permeability of domperidone, metoprolol tartarate and phenol red. Journal of Research in Pharmacy 27 3 967–979.
IEEE S. Özcan, E. Ş. Çağlar, M. S. Kaynak, and N. Üstündağ, “A newly developed and fully validated HPLC method for simultenous determination of in-situ single pass intestinal permeability of domperidone, metoprolol tartarate and phenol red”, J. Res. Pharm., vol. 27, no. 3, pp. 967–979, 2025.
ISNAD Özcan, Saniye et al. “A Newly Developed and Fully Validated HPLC Method for Simultenous Determination of in-Situ Single Pass Intestinal Permeability of Domperidone, Metoprolol Tartarate and Phenol Red”. Journal of Research in Pharmacy 27/3 (June 2025), 967-979.
JAMA Özcan S, Çağlar EŞ, Kaynak MS, Üstündağ N. A newly developed and fully validated HPLC method for simultenous determination of in-situ single pass intestinal permeability of domperidone, metoprolol tartarate and phenol red. J. Res. Pharm. 2025;27:967–979.
MLA Özcan, Saniye et al. “A Newly Developed and Fully Validated HPLC Method for Simultenous Determination of in-Situ Single Pass Intestinal Permeability of Domperidone, Metoprolol Tartarate and Phenol Red”. Journal of Research in Pharmacy, vol. 27, no. 3, 2025, pp. 967-79.
Vancouver Özcan S, Çağlar EŞ, Kaynak MS, Üstündağ N. A newly developed and fully validated HPLC method for simultenous determination of in-situ single pass intestinal permeability of domperidone, metoprolol tartarate and phenol red. J. Res. Pharm. 2025;27(3):967-79.