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Synthesis, In Silico and Structural Insight of Flavonol Derivative Compounds as New Competitive Dengue NS2B/NS3 Protease Inhibitor

Year 2023, Volume: 27 Issue: 3, 1157 - 1169, 28.06.2025

Neni Frimayanti , Ihsan Ikhtiarudin , Abdi Wira Septama Adriani Susanty Nia Daiatul Isroq

Abstract

This paper conducts green synthesis of silver nanoparticles using Pistacia palaestina fruit extract and studies the effects of the fabrication conditions on the size of fabricated nanoparticles during the synthesis process and the evaluation of its wound healing activity in vivo. Methods: P. palaestina extract has been used to reduce silver nitrate solution to AgNPs. The synthesized AgNPs were characterized using UV-Vis spectroscopy, Dynamic Light Scattering (DLS), Atomic Force Microscopy (AFM), and Scanning Electron Microscopy (SEM). The fabricated AgNPs were prepared as an ointment (1% w/w) and evaluated for wound healing activity using an incision wound model on rats. The time of healing was determined and compared with a positive control (gentamicin ointment 0.1%) and a negative control. Results: The fabricated AgNPs have a dark brown color, and their absorption peak was at 430 nm. The obtained particles have an average diameter of 36 nm by SEM, 55.64 nm by DLS, and 100 nm by AFM. AFM and SEM images confirmed the spherical shape of prepared AgNPs. The optimal formula for preparing AgNPs was by using fruit extract (pH 7) at a ratio of (1:9) at 35°C for 24 h. The prepared AgNPs ointment (1%) accelerated wound healing compared to a negative control group, as the time of healing was 9 ± 1.41, 12 ± 1.73, and 17 ± 2.00 days, respectively. Conclusion: It is concluded that P. palaestina extract can be utilized for fabricating AgNPs without any additions, and they have wound healing effect in experimental rats.

Keywords

Dengue NS2B/NS3 Docking ADME Density functional theory

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There are 24 citations in total.

Details

Primary Language English
Subjects Pharmaceutical Chemistry
Journal Section Articles
Authors

Neni Frimayanti

Ihsan Ikhtiarudin

Abdi Wira Septama

Adriani Susanty

Nia Daiatul Isroq

Publication Date June 28, 2025
Published in Issue Year 2023 Volume: 27 Issue: 3

Cite

APA Frimayanti, N., Ikhtiarudin, I., Septama, A. W., Susanty, A., et al. (2025). Synthesis, In Silico and Structural Insight of Flavonol Derivative Compounds as New Competitive Dengue NS2B/NS3 Protease Inhibitor. Journal of Research in Pharmacy, 27(3), 1157-1169.
AMA Frimayanti N, Ikhtiarudin I, Septama AW, Susanty A, Isroq ND. Synthesis, In Silico and Structural Insight of Flavonol Derivative Compounds as New Competitive Dengue NS2B/NS3 Protease Inhibitor. J. Res. Pharm. June 2025;27(3):1157-1169.
Chicago Frimayanti, Neni, Ihsan Ikhtiarudin, Abdi Wira Septama, Adriani Susanty, and Nia Daiatul Isroq. “Synthesis, In Silico and Structural Insight of Flavonol Derivative Compounds As New Competitive Dengue NS2B/NS3 Protease Inhibitor”. Journal of Research in Pharmacy 27, no. 3 (June 2025): 1157-69.
EndNote Frimayanti N, Ikhtiarudin I, Septama AW, Susanty A, Isroq ND (June 1, 2025) Synthesis, In Silico and Structural Insight of Flavonol Derivative Compounds as New Competitive Dengue NS2B/NS3 Protease Inhibitor. Journal of Research in Pharmacy 27 3 1157–1169.
IEEE N. Frimayanti, I. Ikhtiarudin, A. W. Septama, A. Susanty, and N. D. Isroq, “Synthesis, In Silico and Structural Insight of Flavonol Derivative Compounds as New Competitive Dengue NS2B/NS3 Protease Inhibitor”, J. Res. Pharm., vol. 27, no. 3, pp. 1157–1169, 2025.
ISNAD Frimayanti, Neni et al. “Synthesis, In Silico and Structural Insight of Flavonol Derivative Compounds As New Competitive Dengue NS2B/NS3 Protease Inhibitor”. Journal of Research in Pharmacy 27/3 (June 2025), 1157-1169.
JAMA Frimayanti N, Ikhtiarudin I, Septama AW, Susanty A, Isroq ND. Synthesis, In Silico and Structural Insight of Flavonol Derivative Compounds as New Competitive Dengue NS2B/NS3 Protease Inhibitor. J. Res. Pharm. 2025;27:1157–1169.
MLA Frimayanti, Neni et al. “Synthesis, In Silico and Structural Insight of Flavonol Derivative Compounds As New Competitive Dengue NS2B/NS3 Protease Inhibitor”. Journal of Research in Pharmacy, vol. 27, no. 3, 2025, pp. 1157-69.
Vancouver Frimayanti N, Ikhtiarudin I, Septama AW, Susanty A, Isroq ND. Synthesis, In Silico and Structural Insight of Flavonol Derivative Compounds as New Competitive Dengue NS2B/NS3 Protease Inhibitor. J. Res. Pharm. 2025;27(3):1157-69.