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Year 2021, Volume: 25 Issue: 3, 230 - 237, 27.06.2025

Habiba Mokaddem-daroui Maya Belhadj Mostefa Fatma Aydogmus-ozturk Ebru Erol , Mehmet Öztürk , Abdulselam Ertaş , Mehmet Emin Duru , Ahmed Kabouche Zahia Kabouche Kabouche

https://doi.org/10.29228/jrp.13
Cited By: 3

Abstract

References

  • [1] Farjon A, Rushforth KD. A classification of Abies Miller (Pinaceae). Notes from the Royal Botanic Garden, Edinburgh1989; 46(1): 59-79.
  • [2] Uçar G, Uçar MB. Geographical Variation in the Composition of Abies bornmuelleriana Mattf. Needle Oils. Rec Nat Prod. 2014; 8(1): 56-60.
  • [3] Baydar SN. Encyclopedia of Medicinal Plants, Palme Publishing, Istanbul, Turkey 2006.
  • [4] Li YL, Gao YX, Jin HZ, Shan L, Chang WL, Yang XW, Zeng HW, Wang N, Steinmetz A, Zhang WD. Chemical Constituents of Abies fabri. Phytochemistry 2015; 117: 135-143.[CrossRef]
  • [5] Tiwari KP, Minocha PK. A Chalcone Glycoside from Abies pindrow. Phytochemistry 1980; 19(11): 2501-2503.[CrossRef]
  • [6] Xia JH, Zhang SD, Li YL, Wu L, Zhu ZJ, Yang XW, Zeng HW, Li HL, Wang N, Steinmetz A, Zhang WD. Sesquiterpenoids and Triterpenoids from Abies holophylla and Their Bioactivities. Phytochemistry 2012; 74: 178-184.[CrossRef]
  • [7] Lavoie S, Gauthier C, Legault J, Mercier S, Mshvildadze V, Pichette A. Lanostane- and Cycloartane-Type Triterpenoids from Abies balsamea Oleoresin. Beilstein J Org Chem. 2013; 9: 1333-1339. [CrossRef]
  • [8] Raldugin VA, Shevtsov SA. Triterpenoids of Plant of the Genus Abies Hill. Chem Nat Comp. 1990; 26: 373-382.
  • [9] Yang XW, Li SM, Shen YH, Zhang WD, 2008. Phytochemical and Biological Studies of Abies species. Chem Biodivers.2008; 5(1): 56-81. [CrossRef]
  • [10] Benouchenne D, Bellil I, Akkal S, Bensouici C, Khelifi D. LC–MS/MS Analysis, Antioxidant and Antibacterial Activities of Algerian Fir (Abies numidica de LANNOY ex CARRIÈRE) ethyl acetate fraction extracted from needles. J King Saud Univ – Sci. 2020; 32: 3321–3327. [CrossRef]
  • [11] Belhadj-Mostefa M, Abedini A, Voutquenne-Nazabadioko L, Gangloff SC, Kabouche A, Kabouche Z. Abietane Diterpenes from the Cones of Abies numidica de Lannoy ex Carrière (Pinaceae) and In Vitro Evaluation of Their Antimicrobial Properties. Nat Prod Res. 2016; 31(5): 568-571. [CrossRef]
  • [12] Benmerache A, Benteldjoune M, Alabdulmagid A, Abedini A, Berrehal D, Kabouche A, Gangloff SC, Voutquenne-Nazabadioko L, Kabouche Z. Chemical Composition, Antioxidant and Antibacterial Activities of Tamarix balansae J. Gay Aerial parts. Nat Prod Res. 2017; 31(24): 2828-2835. [CrossRef]
  • [13] Bouratoua A, Khalfallah A, Bensouici C, Kabouche Z, Alabdul Magid A, Harakat D, Voutquenne-Nazabadioko L, Kabouche A. Chemical Composition and Antioxidant Activity of Aerial Parts of Ferula longipes Coss. ex Bonnier & Maury. Nat Prod Res. 2018; 32(16): 1873-1880. [CrossRef]
  • [14] Cherchar H, Lehbili M, Berrehal D, Morjani H, Alabdul Magid A, Voutquenne-Nazabadioko L, Kabouche A, Kabouche A. A New 2-Alkylhydroquinone Glucoside from Phagnalon saxatile (L.) Cass. Nat Prod Res. 2018; 32(9):1010-1016. [CrossRef]
  • [15] Lehbili MA, AlabdulMagid A, Kabouche A, Voutquenne-Nazabadioko L, Abedini A, Morjani H, Gangloff SC, Kabouche Z. Antibacterial, Antioxidant and Cytotoxic Activities of Triterpenes and Flavonoids from the Aerial Parts of Salvia barrelieri Etl. Nat Prod Res. 2018; 32(22): 2683-2691. [CrossRef]
  • [16] Bensouici C, Kabouche A, Karioti A, Öztürk M, Duru ME, Bilia AR, Kabouche Z. Compounds From Sedum caeruleumwith Antioxidant, Anticholinesterase, and Antibacterial Activities. Pharm Biol. 2016; 54(1): 174-179. [CrossRef]
  • [17] Labed-Zouad I, Ferhat M, Öztürk M, Abaza I, Nadeem S, Kabouche A, Kabouche Z. Essential Oils Composition, Anticholinesterase and Antioxidant Activities of Pistacia atlantica Desf. Rec Nat Prod. 2017; 11(4): 411-415.
  • [18] Jiang Y, Han W, Shen T, Wang M-H. Antioxidant Activity and Protection from DNA Damage by Water Extract From Pine (Pinus densiflora) Bark. Prev Nutr Food Sci. 2012; 17(2): 116-121. [CrossRef]
  • [19] Tel G, Öztürk M, Duru ME, Dogan B, Harmandar M. Fatty Acid Composition, Antioxidant, Anticholinesterase and Tyrosinase inhibitory Activities of Four Serratula Species from Anatolia. Rec Nat Prod. 2013; 7(2): 86-95.
  • [20] Ertas A, Boga M, Yılmaz MA, Yesil Y, Tel G, Temel H, Hasimi N, Gazioglu I, Ozturk M, Ugurlu P. A Detailed Study on the Chemical and Biological Profiles of Essential Oil and Methanol Extract of Thymus nummularius (Anzer tea): Rosmarinic Acid. Ind Crop Prod. 2015; 67: 336-345. [CrossRef]
  • [21] Miller HE. A Simplified Method for the Evaluation of Antioxidants. J Am Oil Chem Soc. 1971; 48: 91-105. [CrossRef]
  • [22] Kabouche A, Kabouche Z, Öztürk M, Kolak U, Topçu G. Antioxidant Abietane Diterpenoids from Salvia barrelieri. Food Chem. 2007; 102(4): 1281-1287. [CrossRef]
  • [23] Apak R, Güçlü K, Özyürek M, Karademir SE. Novel Total Antioxidant Capacity Index for Dietary Polyphenols and Vitamins C and E, Using Their Cupric Ion Reducing Capability in the Presence of Neocuproine: CUPRAC Method. J Agric Food Chem. 2004; 52(26): 7970-7981. [CrossRef]
  • [24] Blois MS. Antioxidant Determinations by the Use of a Stable Free Radical. Nature 1958; 181: 1199-1200.
  • [25] Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant Activity Applying an Improved ABTS Radical Cation Decolorization Assay. Free Radic Biol Med. 1989; 26(9-10): 1231-1237. [CrossRef]
  • [26] Demirkiran O, Sabudak T, Ozturk M, Topcu G. Antioxidant and Tyrosinase Inhibitory Activities of Flavonoids from Trifolium nigrescens subsp. petrisavi. J Agric Food Chem. 2013; 61(51): 12598-12603. [CrossRef]
  • [27] Ellman GL, Courtney KD, Andres V, Featherston RM. A New and Rapid Colorimetric Determination of Acetylcholinesterase Activity. Biochem Pharmacol. 1961; 7(2): 88-95. [CrossRef]
  • [28] Topcu G, Kolak U, Ozturk M, Boga M, Hatipoglu SD, Bahadori F, Culhaoglu B, Dirmenci T. Investigation of Anticholinesterase Activity of a Series of Salvia Extracts and the Constituents of Salvia staminea. The Nat Prod J. 2013; 3(1): 3-9.
  • [29] Slinkard K, Singleton VL. Total Phenol Analyses: Automation and Comparison with Manual Methods. Am J Enol Vitic. 1977; 28: 49-55.

Antioxidant, anticholinesterase activities and polyphenolic constituents of cones of algerian fir (Abies numidica) by LC- ESI-MS/MS with chemometric approach

Year 2021, Volume: 25 Issue: 3, 230 - 237, 27.06.2025

Habiba Mokaddem-daroui Maya Belhadj Mostefa Fatma Aydogmus-ozturk Ebru Erol , Mehmet Öztürk , Abdulselam Ertaş , Mehmet Emin Duru , Ahmed Kabouche Zahia Kabouche Kabouche

https://doi.org/10.29228/jrp.13
Cited By: 3

Abstract

The fractions (Fr.1-5) of the hydromethanolic extract of Abies numidica cones were studied for their polyphenol constituents, antioxidant, and anticholinesterase activities. The β-carotene-linoleic acid, cupric reducing power (CUPRAC), DPPH scavenging, and ABTS radical scavenging assays were used to determine antioxidant activity. Fr.3 exhibited the highest antioxidant activity in ABTS test (IC50: 1.29 µg/mL), b-carotene-linoleic acid test (IC50: 18.6 µg/mL) and CUPRAC (A0.5: 33.8 µg/mL) assays. Three fractions (Fr.2-4) promising antioxidant activity were analyzed using LC-MS/MS for their phenolic compositions. Taxifolin (155.9-2816.2 µg analyte/g extract), hyperoside (353.0- 2045.5 µg/g), vanillin (1488.9-1529.9 µg/g), tannic acid (1281.6-1416.8 µg/g), rosmarinic acid (1063.1-1149.3 µg/g), coumarin (683.0-772.6 µg/g), quercetin (881.0 µg/g), and catechin (277.7-400.2 μg/g) were identified in the antioxidant fractions. Moreover, the anticholinesterase activity was also performed using the in vitro spectroscopic Ellman method. The antioxidant fractions exhibited moderate butyrylcholinesterase inhibitory activity. The multi-ware analysis was performed to understand the origin of bioactivities. According to principal component analysis, it is detected that the hyperoside, catechin, taxifolin, and tannic acid were responsible for the antioxidant activity. Therefore, the cones of Abies numidica can be considered as potent antioxidant and anticholinesterase sources in food and pharmaceutical industries, commercially.

Keywords

Abies numidica LC-MS/MS antioxidant activity anticholinesterase activity taxifolin hyperoside

References

  • [1] Farjon A, Rushforth KD. A classification of Abies Miller (Pinaceae). Notes from the Royal Botanic Garden, Edinburgh1989; 46(1): 59-79.
  • [2] Uçar G, Uçar MB. Geographical Variation in the Composition of Abies bornmuelleriana Mattf. Needle Oils. Rec Nat Prod. 2014; 8(1): 56-60.
  • [3] Baydar SN. Encyclopedia of Medicinal Plants, Palme Publishing, Istanbul, Turkey 2006.
  • [4] Li YL, Gao YX, Jin HZ, Shan L, Chang WL, Yang XW, Zeng HW, Wang N, Steinmetz A, Zhang WD. Chemical Constituents of Abies fabri. Phytochemistry 2015; 117: 135-143.[CrossRef]
  • [5] Tiwari KP, Minocha PK. A Chalcone Glycoside from Abies pindrow. Phytochemistry 1980; 19(11): 2501-2503.[CrossRef]
  • [6] Xia JH, Zhang SD, Li YL, Wu L, Zhu ZJ, Yang XW, Zeng HW, Li HL, Wang N, Steinmetz A, Zhang WD. Sesquiterpenoids and Triterpenoids from Abies holophylla and Their Bioactivities. Phytochemistry 2012; 74: 178-184.[CrossRef]
  • [7] Lavoie S, Gauthier C, Legault J, Mercier S, Mshvildadze V, Pichette A. Lanostane- and Cycloartane-Type Triterpenoids from Abies balsamea Oleoresin. Beilstein J Org Chem. 2013; 9: 1333-1339. [CrossRef]
  • [8] Raldugin VA, Shevtsov SA. Triterpenoids of Plant of the Genus Abies Hill. Chem Nat Comp. 1990; 26: 373-382.
  • [9] Yang XW, Li SM, Shen YH, Zhang WD, 2008. Phytochemical and Biological Studies of Abies species. Chem Biodivers.2008; 5(1): 56-81. [CrossRef]
  • [10] Benouchenne D, Bellil I, Akkal S, Bensouici C, Khelifi D. LC–MS/MS Analysis, Antioxidant and Antibacterial Activities of Algerian Fir (Abies numidica de LANNOY ex CARRIÈRE) ethyl acetate fraction extracted from needles. J King Saud Univ – Sci. 2020; 32: 3321–3327. [CrossRef]
  • [11] Belhadj-Mostefa M, Abedini A, Voutquenne-Nazabadioko L, Gangloff SC, Kabouche A, Kabouche Z. Abietane Diterpenes from the Cones of Abies numidica de Lannoy ex Carrière (Pinaceae) and In Vitro Evaluation of Their Antimicrobial Properties. Nat Prod Res. 2016; 31(5): 568-571. [CrossRef]
  • [12] Benmerache A, Benteldjoune M, Alabdulmagid A, Abedini A, Berrehal D, Kabouche A, Gangloff SC, Voutquenne-Nazabadioko L, Kabouche Z. Chemical Composition, Antioxidant and Antibacterial Activities of Tamarix balansae J. Gay Aerial parts. Nat Prod Res. 2017; 31(24): 2828-2835. [CrossRef]
  • [13] Bouratoua A, Khalfallah A, Bensouici C, Kabouche Z, Alabdul Magid A, Harakat D, Voutquenne-Nazabadioko L, Kabouche A. Chemical Composition and Antioxidant Activity of Aerial Parts of Ferula longipes Coss. ex Bonnier & Maury. Nat Prod Res. 2018; 32(16): 1873-1880. [CrossRef]
  • [14] Cherchar H, Lehbili M, Berrehal D, Morjani H, Alabdul Magid A, Voutquenne-Nazabadioko L, Kabouche A, Kabouche A. A New 2-Alkylhydroquinone Glucoside from Phagnalon saxatile (L.) Cass. Nat Prod Res. 2018; 32(9):1010-1016. [CrossRef]
  • [15] Lehbili MA, AlabdulMagid A, Kabouche A, Voutquenne-Nazabadioko L, Abedini A, Morjani H, Gangloff SC, Kabouche Z. Antibacterial, Antioxidant and Cytotoxic Activities of Triterpenes and Flavonoids from the Aerial Parts of Salvia barrelieri Etl. Nat Prod Res. 2018; 32(22): 2683-2691. [CrossRef]
  • [16] Bensouici C, Kabouche A, Karioti A, Öztürk M, Duru ME, Bilia AR, Kabouche Z. Compounds From Sedum caeruleumwith Antioxidant, Anticholinesterase, and Antibacterial Activities. Pharm Biol. 2016; 54(1): 174-179. [CrossRef]
  • [17] Labed-Zouad I, Ferhat M, Öztürk M, Abaza I, Nadeem S, Kabouche A, Kabouche Z. Essential Oils Composition, Anticholinesterase and Antioxidant Activities of Pistacia atlantica Desf. Rec Nat Prod. 2017; 11(4): 411-415.
  • [18] Jiang Y, Han W, Shen T, Wang M-H. Antioxidant Activity and Protection from DNA Damage by Water Extract From Pine (Pinus densiflora) Bark. Prev Nutr Food Sci. 2012; 17(2): 116-121. [CrossRef]
  • [19] Tel G, Öztürk M, Duru ME, Dogan B, Harmandar M. Fatty Acid Composition, Antioxidant, Anticholinesterase and Tyrosinase inhibitory Activities of Four Serratula Species from Anatolia. Rec Nat Prod. 2013; 7(2): 86-95.
  • [20] Ertas A, Boga M, Yılmaz MA, Yesil Y, Tel G, Temel H, Hasimi N, Gazioglu I, Ozturk M, Ugurlu P. A Detailed Study on the Chemical and Biological Profiles of Essential Oil and Methanol Extract of Thymus nummularius (Anzer tea): Rosmarinic Acid. Ind Crop Prod. 2015; 67: 336-345. [CrossRef]
  • [21] Miller HE. A Simplified Method for the Evaluation of Antioxidants. J Am Oil Chem Soc. 1971; 48: 91-105. [CrossRef]
  • [22] Kabouche A, Kabouche Z, Öztürk M, Kolak U, Topçu G. Antioxidant Abietane Diterpenoids from Salvia barrelieri. Food Chem. 2007; 102(4): 1281-1287. [CrossRef]
  • [23] Apak R, Güçlü K, Özyürek M, Karademir SE. Novel Total Antioxidant Capacity Index for Dietary Polyphenols and Vitamins C and E, Using Their Cupric Ion Reducing Capability in the Presence of Neocuproine: CUPRAC Method. J Agric Food Chem. 2004; 52(26): 7970-7981. [CrossRef]
  • [24] Blois MS. Antioxidant Determinations by the Use of a Stable Free Radical. Nature 1958; 181: 1199-1200.
  • [25] Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant Activity Applying an Improved ABTS Radical Cation Decolorization Assay. Free Radic Biol Med. 1989; 26(9-10): 1231-1237. [CrossRef]
  • [26] Demirkiran O, Sabudak T, Ozturk M, Topcu G. Antioxidant and Tyrosinase Inhibitory Activities of Flavonoids from Trifolium nigrescens subsp. petrisavi. J Agric Food Chem. 2013; 61(51): 12598-12603. [CrossRef]
  • [27] Ellman GL, Courtney KD, Andres V, Featherston RM. A New and Rapid Colorimetric Determination of Acetylcholinesterase Activity. Biochem Pharmacol. 1961; 7(2): 88-95. [CrossRef]
  • [28] Topcu G, Kolak U, Ozturk M, Boga M, Hatipoglu SD, Bahadori F, Culhaoglu B, Dirmenci T. Investigation of Anticholinesterase Activity of a Series of Salvia Extracts and the Constituents of Salvia staminea. The Nat Prod J. 2013; 3(1): 3-9.
  • [29] Slinkard K, Singleton VL. Total Phenol Analyses: Automation and Comparison with Manual Methods. Am J Enol Vitic. 1977; 28: 49-55.
There are 29 citations in total.

Details

Primary Language English
Subjects Pharmaceutical Sciences, Pharmacognosy, Pharmacology and Pharmaceutical Sciences (Other)
Journal Section Articles
Authors

Habiba Mokaddem-daroui

Maya Belhadj Mostefa

Fatma Aydogmus-ozturk

Ebru Erol

Mehmet Öztürk

Abdulselam Ertaş

Mehmet Emin Duru

Ahmed Kabouche

Zahia Kabouche Kabouche

Publication Date June 27, 2025
Published in Issue Year 2021 Volume: 25 Issue: 3

Cite

APA Mokaddem-daroui, H., Belhadj Mostefa, M., Aydogmus-ozturk, F., Erol, E., et al. (2025). Antioxidant, anticholinesterase activities and polyphenolic constituents of cones of algerian fir (Abies numidica) by LC- ESI-MS/MS with chemometric approach. Journal of Research in Pharmacy, 25(3), 230-237. https://doi.org/10.29228/jrp.13
AMA Mokaddem-daroui H, Belhadj Mostefa M, Aydogmus-ozturk F, Erol E, Öztürk M, Ertaş A, Duru ME, Kabouche A, Kabouche ZK. Antioxidant, anticholinesterase activities and polyphenolic constituents of cones of algerian fir (Abies numidica) by LC- ESI-MS/MS with chemometric approach. J. Res. Pharm. June 2025;25(3):230-237. doi:10.29228/jrp.13
Chicago Mokaddem-daroui, Habiba, Maya Belhadj Mostefa, Fatma Aydogmus-ozturk, Ebru Erol, Mehmet Öztürk, Abdulselam Ertaş, Mehmet Emin Duru, Ahmed Kabouche, and Zahia Kabouche Kabouche. “Antioxidant, Anticholinesterase Activities and Polyphenolic Constituents of Cones of Algerian Fir (Abies Numidica) by LC- ESI-MS/MS With Chemometric Approach”. Journal of Research in Pharmacy 25, no. 3 (June 2025): 230-37. https://doi.org/10.29228/jrp.13.
EndNote Mokaddem-daroui H, Belhadj Mostefa M, Aydogmus-ozturk F, Erol E, Öztürk M, Ertaş A, Duru ME, Kabouche A, Kabouche ZK (June 1, 2025) Antioxidant, anticholinesterase activities and polyphenolic constituents of cones of algerian fir (Abies numidica) by LC- ESI-MS/MS with chemometric approach. Journal of Research in Pharmacy 25 3 230–237.
IEEE H. Mokaddem-daroui, M. Belhadj Mostefa, F. Aydogmus-ozturk, E. Erol, M. Öztürk, A. Ertaş, M. E. Duru, A. Kabouche, and Z. K. Kabouche, “Antioxidant, anticholinesterase activities and polyphenolic constituents of cones of algerian fir (Abies numidica) by LC- ESI-MS/MS with chemometric approach”, J. Res. Pharm., vol. 25, no. 3, pp. 230–237, 2025, doi: 10.29228/jrp.13.
ISNAD Mokaddem-daroui, Habiba et al. “Antioxidant, Anticholinesterase Activities and Polyphenolic Constituents of Cones of Algerian Fir (Abies Numidica) by LC- ESI-MS/MS With Chemometric Approach”. Journal of Research in Pharmacy 25/3 (June 2025), 230-237. https://doi.org/10.29228/jrp.13.
JAMA Mokaddem-daroui H, Belhadj Mostefa M, Aydogmus-ozturk F, Erol E, Öztürk M, Ertaş A, Duru ME, Kabouche A, Kabouche ZK. Antioxidant, anticholinesterase activities and polyphenolic constituents of cones of algerian fir (Abies numidica) by LC- ESI-MS/MS with chemometric approach. J. Res. Pharm. 2025;25:230–237.
MLA Mokaddem-daroui, Habiba et al. “Antioxidant, Anticholinesterase Activities and Polyphenolic Constituents of Cones of Algerian Fir (Abies Numidica) by LC- ESI-MS/MS With Chemometric Approach”. Journal of Research in Pharmacy, vol. 25, no. 3, 2025, pp. 230-7, doi:10.29228/jrp.13.
Vancouver Mokaddem-daroui H, Belhadj Mostefa M, Aydogmus-ozturk F, Erol E, Öztürk M, Ertaş A, Duru ME, Kabouche A, Kabouche ZK. Antioxidant, anticholinesterase activities and polyphenolic constituents of cones of algerian fir (Abies numidica) by LC- ESI-MS/MS with chemometric approach. J. Res. Pharm. 2025;25(3):230-7.

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