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Bortezomib synergizes with nocodazole in p53 mediated DNA damage response signalling in A549 lung cancer cells

Year 2021, Volume: 25 Issue: 3, 287 - 291, 27.06.2025

Gülşah Albayrak

Abstract

The adoption of new treatment modalities is required to improve outcomes in lung cancer treatment as lung cancer has lowest survival rates, along with liver and pancreatic cancer. Bortezomib is a proteasome inhibitor that has higher anticancer effect in combination therapies. The aim of this study was to investigate whether bortezomib could have additional anticancer effect with antineoplastic tubulin binding agent -nocodazole in A549 lung cancer cells. Apoptosis related gene expression levels of Noxa, Bcl-xL, Casp3 and Casp7 were measured by real-time PCR after treatment with 30 nM bortezomib, 0.3 μg/ml nocodazole and with their combination for 24 hours. Synergistic effect on DNA damage response was investigated at protein levels by checking p53 and cleaved PARP expressions. Induction of apoptotis was determined at protein expression level by western blotting of XIAP, Bcl-X and Bim. It was found that nocodazole combined bortezomib treatment induced apoptosis via p53 mediated DNA damage response signalling. P53 and cleaved PARP protein expressions were increased significantly after combination treatment. Apoptosis related genes Noxa, Casp3 and Casp7 mRNA expressions were elevated significantly after combination treatment. This study concludes that bortezomib potentiates the effect of nocodazole via DNA damage induced apoptosis in A549 lung cancer cells.

Keywords

Bortezomib Nocodazole DNA damage lung cancer

References

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There are 20 citations in total.

Details

Primary Language English
Subjects Pharmacology and Pharmaceutical Sciences (Other)
Journal Section Articles
Authors

Gülşah Albayrak

Publication Date June 27, 2025
Published in Issue Year 2021 Volume: 25 Issue: 3

Cite

APA Albayrak, G. (2025). Bortezomib synergizes with nocodazole in p53 mediated DNA damage response signalling in A549 lung cancer cells. Journal of Research in Pharmacy, 25(3), 287-291.
AMA Albayrak G. Bortezomib synergizes with nocodazole in p53 mediated DNA damage response signalling in A549 lung cancer cells. J. Res. Pharm. June 2025;25(3):287-291.
Chicago Albayrak, Gülşah. “Bortezomib Synergizes With Nocodazole in p53 Mediated DNA Damage Response Signalling in A549 Lung Cancer Cells”. Journal of Research in Pharmacy 25, no. 3 (June 2025): 287-91.
EndNote Albayrak G (June 1, 2025) Bortezomib synergizes with nocodazole in p53 mediated DNA damage response signalling in A549 lung cancer cells. Journal of Research in Pharmacy 25 3 287–291.
IEEE G. Albayrak, “Bortezomib synergizes with nocodazole in p53 mediated DNA damage response signalling in A549 lung cancer cells”, J. Res. Pharm., vol. 25, no. 3, pp. 287–291, 2025.
ISNAD Albayrak, Gülşah. “Bortezomib Synergizes With Nocodazole in p53 Mediated DNA Damage Response Signalling in A549 Lung Cancer Cells”. Journal of Research in Pharmacy 25/3 (June 2025), 287-291.
JAMA Albayrak G. Bortezomib synergizes with nocodazole in p53 mediated DNA damage response signalling in A549 lung cancer cells. J. Res. Pharm. 2025;25:287–291.
MLA Albayrak, Gülşah. “Bortezomib Synergizes With Nocodazole in p53 Mediated DNA Damage Response Signalling in A549 Lung Cancer Cells”. Journal of Research in Pharmacy, vol. 25, no. 3, 2025, pp. 287-91.
Vancouver Albayrak G. Bortezomib synergizes with nocodazole in p53 mediated DNA damage response signalling in A549 lung cancer cells. J. Res. Pharm. 2025;25(3):287-91.