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Evaluation of in vitro antioxidants activities, hepatoprotective and haematological effects of ethanol extract of Anthocleista vogelii stem bark (AVSB) on carbon tetrachloride (CCl4) induced rats

Year 2021, Volume: 25 Issue: 3, 258 - 270, 27.06.2025

Robert Uroko Chinedu Nwuke Amarachi Agbafor Josephat Okwor

Abstract

Anthocleista vogelii stem bark extract (AVSB) is used as a hepatoprotective protective and blood-boosting agent locally without any scientific evidence. This study evaluated the in vitro antioxidants activity, hepatoprotective and haematological effects of ethanol extract of AVSB on carbon tetrachloride (CCl4) induced rats. Thirty rats distributed into 5 groups (n = 6) were used. Group 1 was the normal control rats without CCl4 induction, group 2 was the CCl4 control (CCl4 induced untreated rats) while groups 3 – 6 were CCl4 induced rats treated with 100 mg/kg/day of silymarin, 100, 200 and 500 mg/kg/day of AVSB respectively for 14 days. The results indicated high levels of total phenols, flavonoid, vitamin E, vitamin C, -carotene and lycopene in the AVSB. The AVSB exhibited a dose-dependent increase in ferric reducing antioxidant power, DPPH and nitric oxide radicals scavenging activities similar to vitamin C, rutin and curcumin respectively. The CCl4 induction significantly (P<0.05) elevated total bilirubin concentrations, serum ALT, AST and ALP activities and significantly (P<0.05) decreased PCV, RBC, WBC, platelet counts, and Hb, total protein and albumin concentrations and caused liver necrosis in the untreated rats relative to the normal control. Treatment with AVSB significantly reversed the altered serum ALT, AST and ALP activities, total bilirubin, total protein, albumin and Hb concentrations including haematological indices and liver histomorphology to normal compared with the CCl4 control. The findings of this study indicated that the AVSB is rich in antioxidant components, in vitro antioxidant activity, and possesses hepatoprotective and improves haematological indices

Keywords

Anthocleista vogelii antioxidant activity carbon tetrachloride hepatoprotective haematological indices.

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There are 37 citations in total.

Details

Primary Language English
Subjects Pharmaceutical Biochemistry, Pharmacognosy
Journal Section Articles
Authors

Robert Uroko

Chinedu Nwuke

Amarachi Agbafor

Josephat Okwor

Publication Date June 27, 2025
Published in Issue Year 2021 Volume: 25 Issue: 3

Cite

APA Uroko, R., Nwuke, C., Agbafor, A., Okwor, J. (2025). Evaluation of in vitro antioxidants activities, hepatoprotective and haematological effects of ethanol extract of Anthocleista vogelii stem bark (AVSB) on carbon tetrachloride (CCl4) induced rats. Journal of Research in Pharmacy, 25(3), 258-270.
AMA Uroko R, Nwuke C, Agbafor A, Okwor J. Evaluation of in vitro antioxidants activities, hepatoprotective and haematological effects of ethanol extract of Anthocleista vogelii stem bark (AVSB) on carbon tetrachloride (CCl4) induced rats. J. Res. Pharm. June 2025;25(3):258-270.
Chicago Uroko, Robert, Chinedu Nwuke, Amarachi Agbafor, and Josephat Okwor. “Evaluation of in Vitro Antioxidants Activities, Hepatoprotective and Haematological Effects of Ethanol Extract of Anthocleista Vogelii Stem Bark (AVSB) on Carbon Tetrachloride (CCl4) Induced Rats”. Journal of Research in Pharmacy 25, no. 3 (June 2025): 258-70.
EndNote Uroko R, Nwuke C, Agbafor A, Okwor J (June 1, 2025) Evaluation of in vitro antioxidants activities, hepatoprotective and haematological effects of ethanol extract of Anthocleista vogelii stem bark (AVSB) on carbon tetrachloride (CCl4) induced rats. Journal of Research in Pharmacy 25 3 258–270.
IEEE R. Uroko, C. Nwuke, A. Agbafor, and J. Okwor, “Evaluation of in vitro antioxidants activities, hepatoprotective and haematological effects of ethanol extract of Anthocleista vogelii stem bark (AVSB) on carbon tetrachloride (CCl4) induced rats”, J. Res. Pharm., vol. 25, no. 3, pp. 258–270, 2025.
ISNAD Uroko, Robert et al. “Evaluation of in Vitro Antioxidants Activities, Hepatoprotective and Haematological Effects of Ethanol Extract of Anthocleista Vogelii Stem Bark (AVSB) on Carbon Tetrachloride (CCl4) Induced Rats”. Journal of Research in Pharmacy 25/3 (June 2025), 258-270.
JAMA Uroko R, Nwuke C, Agbafor A, Okwor J. Evaluation of in vitro antioxidants activities, hepatoprotective and haematological effects of ethanol extract of Anthocleista vogelii stem bark (AVSB) on carbon tetrachloride (CCl4) induced rats. J. Res. Pharm. 2025;25:258–270.
MLA Uroko, Robert et al. “Evaluation of in Vitro Antioxidants Activities, Hepatoprotective and Haematological Effects of Ethanol Extract of Anthocleista Vogelii Stem Bark (AVSB) on Carbon Tetrachloride (CCl4) Induced Rats”. Journal of Research in Pharmacy, vol. 25, no. 3, 2025, pp. 258-70.
Vancouver Uroko R, Nwuke C, Agbafor A, Okwor J. Evaluation of in vitro antioxidants activities, hepatoprotective and haematological effects of ethanol extract of Anthocleista vogelii stem bark (AVSB) on carbon tetrachloride (CCl4) induced rats. J. Res. Pharm. 2025;25(3):258-70.