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Crocin suppressed cold allodynia and anxiety through α2- adrenoceptors in the anterior cingulate cortex following chronic constriction injury of sciatic nerve in rats

Year 2020, Volume: 24 Issue: 6, 833 - 841, 27.06.2025

Mohammad Reza Zohrehvand Reza Kazemi Mohammad Hassan Mirasheh Farideh Bahrami Mohammad Ali Zabihian Gholam Hossein Meftahi Mehdi Rahimi-nasrabadi Zahra Bahari

Abstract

: It is believed that α-adrenoceptors have critical contribution in the process of pain information. Anterior cingulate of cortex (ACC) is a key area of brain associated with pain perception. Pharmacological studies demonstrated that crocin, as a potent antioxidant, has analgesic effects. The underlying analgesic mechanism of the crocin is far from clear. Therefore, the present study was design to examine the interaction of anti-nociceptive effects of crocin with α1- and α2-adrenoceptors of ACC in chronic constriction injury (CCI) model of neuropathic pain. Intra-ACC injection of crocin significantly decreased cold allodynia (using acetone test) and anxiety (using elevated plus maze test) in neuropathic rats from 2 days to 6 days’ post-surgery. Co-injection of crocin and prazosin (α1-adrenoceptors antagonist, 30 μg/5μl) had no effect on the allodynia. However, co-injection of crocin and yohimbine (α2-adrenoceptors antagonist, 30 μg/5μl) significantly increased the allodynia on days 4 and 6 post-surgery as compared with CCI+crocin rats. Moreover, our data identified that neuropathy decreased open arm entries and locomotor activity. Additionally, crocin increased entries to open arms; but this increase was not significant as compared to CCI group. There was no significant difference between CCI+Crocin and CCI+crocin+prazosin groups. However, co-injection of crocin and yohimbine significantly decreased entries to open arms as compared with CCI+crocin group. Furthermore, co-injection of crocin with prazosin or yohimbine did not cause significant changes in locomotor activity. The present study suggested that the anti-nociceptive and anxiolytic effects of crocin appear to be mediated through α2-, and not α1-adrenoceptors in the ACC.

Keywords

Neuropathic pain anterior cingulate cortex crocin prazosin yohimbine

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There are 47 citations in total.

Details

Primary Language English
Subjects Pharmacology and Pharmaceutical Sciences (Other)
Journal Section Articles
Authors

Mohammad Reza Zohrehvand

Reza Kazemi

Mohammad Hassan Mirasheh

Farideh Bahrami

Mohammad Ali Zabihian

Gholam Hossein Meftahi

Mehdi Rahimi-nasrabadi

Zahra Bahari

Publication Date June 27, 2025
Published in Issue Year 2020 Volume: 24 Issue: 6

Cite

APA Zohrehvand, M. R., Kazemi, R., Mirasheh, M. H., Bahrami, F., et al. (2025). Crocin suppressed cold allodynia and anxiety through α2- adrenoceptors in the anterior cingulate cortex following chronic constriction injury of sciatic nerve in rats. Journal of Research in Pharmacy, 24(6), 833-841.
AMA Zohrehvand MR, Kazemi R, Mirasheh MH, Bahrami F, Zabihian MA, Meftahi GH, Rahimi-nasrabadi M, Bahari Z. Crocin suppressed cold allodynia and anxiety through α2- adrenoceptors in the anterior cingulate cortex following chronic constriction injury of sciatic nerve in rats. J. Res. Pharm. June 2025;24(6):833-841.
Chicago Zohrehvand, Mohammad Reza, Reza Kazemi, Mohammad Hassan Mirasheh, Farideh Bahrami, Mohammad Ali Zabihian, Gholam Hossein Meftahi, Mehdi Rahimi-nasrabadi, and Zahra Bahari. “Crocin Suppressed Cold Allodynia and Anxiety through α2- Adrenoceptors in the Anterior Cingulate Cortex Following Chronic Constriction Injury of Sciatic Nerve in Rats”. Journal of Research in Pharmacy 24, no. 6 (June 2025): 833-41.
EndNote Zohrehvand MR, Kazemi R, Mirasheh MH, Bahrami F, Zabihian MA, Meftahi GH, Rahimi-nasrabadi M, Bahari Z (June 1, 2025) Crocin suppressed cold allodynia and anxiety through α2- adrenoceptors in the anterior cingulate cortex following chronic constriction injury of sciatic nerve in rats. Journal of Research in Pharmacy 24 6 833–841.
IEEE M. R. Zohrehvand, R. Kazemi, M. H. Mirasheh, F. Bahrami, M. A. Zabihian, G. H. Meftahi, M. Rahimi-nasrabadi, and Z. Bahari, “Crocin suppressed cold allodynia and anxiety through α2- adrenoceptors in the anterior cingulate cortex following chronic constriction injury of sciatic nerve in rats”, J. Res. Pharm., vol. 24, no. 6, pp. 833–841, 2025.
ISNAD Zohrehvand, Mohammad Reza et al. “Crocin Suppressed Cold Allodynia and Anxiety through α2- Adrenoceptors in the Anterior Cingulate Cortex Following Chronic Constriction Injury of Sciatic Nerve in Rats”. Journal of Research in Pharmacy 24/6 (June 2025), 833-841.
JAMA Zohrehvand MR, Kazemi R, Mirasheh MH, Bahrami F, Zabihian MA, Meftahi GH, Rahimi-nasrabadi M, Bahari Z. Crocin suppressed cold allodynia and anxiety through α2- adrenoceptors in the anterior cingulate cortex following chronic constriction injury of sciatic nerve in rats. J. Res. Pharm. 2025;24:833–841.
MLA Zohrehvand, Mohammad Reza et al. “Crocin Suppressed Cold Allodynia and Anxiety through α2- Adrenoceptors in the Anterior Cingulate Cortex Following Chronic Constriction Injury of Sciatic Nerve in Rats”. Journal of Research in Pharmacy, vol. 24, no. 6, 2025, pp. 833-41.
Vancouver Zohrehvand MR, Kazemi R, Mirasheh MH, Bahrami F, Zabihian MA, Meftahi GH, Rahimi-nasrabadi M, Bahari Z. Crocin suppressed cold allodynia and anxiety through α2- adrenoceptors in the anterior cingulate cortex following chronic constriction injury of sciatic nerve in rats. J. Res. Pharm. 2025;24(6):833-41.