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Year 2020, Volume: 24 Issue: 6, 855 - 864, 27.06.2025

Suvarna Ingale Prasad Upadhye Pramod Ingale

https://doi.org/10.35333/jrp.2020.244

Abstract

References

  • [1] Kelly HW and Sorknes CA. Asthma. In: Dipiro JT, Talbert RL, Yee GC, Matzke TR, Wells BG, Posey LM. (Eds). Pharmacotherapy - A Pathophysiological Approach. The McGraw-Hill, New York, 2005, pp. 504-509.
  • [2] WHO Chronic respiratory diseases: Asthma. http://www.who.int/respiratory/asthma/en/ (accessed on April 20, 2019).
  • [3] Clarke R, Lundy FT, McGarvey L. Herbal treatment in asthma and COPD – current evidence. Clin Phytosci. 2015; 1:4. [CrossRef]
  • [4] Anonymous. Charaka Samhita, Vol 4. Shri Gulabkunverba Ayurvedic Society, Ayurvedic Mundranalaya, Jamangar 1949.
  • [5] Velmurugan V, Shiny G, Surya SP. Phytochemical and Biological Screening of Luffa cylindrica Linn. Fruit. Int J Pharm Tech Res. 2011; 3(3): 1582-1585.
  • [6] Muthumani P, Meera R, Subin M, Jeenamathew, Devi P, Kameswari B, Eswarapriya B. Phytochemical Screening and Anti-inflammatory, Bronchodilator and Antimicrobial activities of the Seeds of Luffa cylindrica. Res J Pharm Biol Chem Sci. 2010; 1(4): 11-22.
  • [7] Zhou X, Wang F, Zhou R, Song X, Xie M. Apigenin: A current review on its beneficial biological activities. J Food Biochem. 2017; 41: e12376. [CrossRef]
  • [8] Shete RV, Pawashe PM, Kore KJ, Otari KV. Protective Role of Luffa cylindrica Linn against Erythromycin Estolate Induced Hepatotoxicity. Current Pharma Research. 2011; 1(4): 315-319. [CrossRef]
  • [9] Harborne JB. Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis. Third ed., Chapman and Hall, London 1998.
  • [10] Marinova D, Ribarova F, Atanassova M. Total phenolics and total flavonoids in bulgarian fruits and vegetables. J Univ Chem Technol Metallurgy. 2005; 40(3): 255-60.
  • [11] Shamsa F, Monsef HR, Ghamooshi R. Spectrophotometric determination of total alkaloids in Peganum harmala L. using bromocresol green. Res J Phytochem. 2007; 1(2): 79-82.
  • [12] OECD, Test No. 423: Acute Oral toxicity - Acute Toxic Class Method, OECD Guidelines for the Testing of Chemicals, Section 4, OECD Publishing, Paris 2002.
  • [13] Nagore DH, Ghosh VK, Patil MJ. Evaluation of antiasthmatic activity of Cassia sophera Linn. Pharmacogn Mag. 2009; 5(19): 109-118.
  • [14] Kumar D, Prasad DN, Parkash J, Bhatnagar SP, Kumar D. Antiasthmatic activity of ethanolic extract of Aerva lanata Linn. Pharmacologyonline. 2009; 2: 1075-1081.
  • [15] Gokhale AB, Saraf MN. Studies on antiallergic activity of ethanolic extract of Tephrosia purpurea Linn. Indian Drugs. 2000; 37(5): 228-232.
  • [16] Lee DS, Park WS, Heo SJ, Cha SH, Kim D, Jeon YJ, Park SG, Seo SK, Choi JS, Park SJ, Shim EB, Choi IW, Jung WK. Polyopes affinis alleviates airway inflammation in a murine model of allergic asthma. J Biosci. 2011; 36(5): 869–877. [CrossRef]
  • [17] National Asthma Education and Prevention Program, Third Expert Panel on the Diagnosis and Management of Asthma. Expert Panel Report 3: Guidelines for the Diagnosis and Management of Asthma. Bethesda (MD): National Heart, Lung, and Blood Institute (US); 2007 Aug. Section 2, Definition, Pathophysiology and Pathogenesis of Asthma, and Natural History of Asthma. Available from: https://www.ncbi.nlm.nih.gov/books/NBK7223/
  • [18] Yamauchi K, Ogasawara M. The Role of Histamine in the Pathophysiology of Asthma and the Clinical Efficacy of Antihistamines in Asthma Therapy. Int J Mol Sci. 2019; 20(7): 1733. [CrossRef]
  • [19] Gosens R, Gross N. The mode of action of anticholinergics in asthma. Eur Respir J. 2018; 52 (4): 1701247. [CrossRef]
  • [20] Jadhav JH, Balsara JJ, Chandorkar AG. Involvement of histaminergic mechanisms in the cataleptogenic effect of clonidine in mice. J Pharm Pharmacol. 1983; 35: 671–673. [CrossRef]
  • [21] Bakakos A, Loukides S, Bakakos P. Severe Eosinophilic Asthma. J Clin Med. 2019; 8(9):1375. [CrossRef]
  • [22] Cockcroft DW. Allergen-induced asthma. Can Respir J. 2014; 21 (5): 279–282. [CrossRef]
  • [23] Aun MV, Bonamichi-Santos R, Arantes-Costa FM, Kalil J, Giavina-Bianchi P. Animal models of asthma: utility and limitations. J Asthma Allergy. 2017; 10: 293–301. [CrossRef]
  • [24] Mathew JE, Srinivasan KK, Dinakaran V, Joseph A. Mast cell stabilizing effects of Sphaeranthus indicus. J Ethnopharmacol. 2009; 122(2): 394–396. [CrossRef]
  • [25] Pungle P, Banavalikar M, Suthar A, Biyani M, Mengi S. Immunomodulatory activity of boswellic acids of Boswellia serrata Roxb. Indian J Exp Biol. 2003; 41(12): 1460-1462.
  • [26] Galli SJ, Grimbaldeston M, Tsai M. Immunomodulatory mast cells: negative, as well as positive, regulators of immunity. Nat Rev Immunol. 2008; 8(6): 478–486. [CrossRef]
  • [27] Busse WW, Rosenwasser LJ. Mechanisms of asthma. J Allergy Clin Immunol. 2003; 111(3): 799–804. [CrossRef]

Evaluation anti-asthmatic activity of hydroalcoholic extract of Luffa cylindrica leaves

Year 2020, Volume: 24 Issue: 6, 855 - 864, 27.06.2025

Suvarna Ingale Prasad Upadhye Pramod Ingale

https://doi.org/10.35333/jrp.2020.244

Abstract

Asthma is an allergic chronic inflammatory disorder of the lung presented clinically in the form bronchial obstruction due to hyper-responsive bronchial wall inflammation and bronchial smooth muscle constriction. Conventional anti-asthmatic drugs are associated with many adverse effcts as well as non-compliance and nonadherence to complicated drug regimens. Thus, to inhibit these adverse effects and to improve patient compliance, there is an unmet medical need for complementary therapies for asthma. Ayurveda has recommended a number of drugs from indigenous plant sources for the treatment of bronchial asthma and allergic disorders. Luffa cylindrica Linn has been traditionally claimed to be used in treatment of bronchitis, bronchial asthma, skin diseases, rheumatoid arthritis, fever, etc. Considering phytochemical profile & traditional claims of Luffa cylindrica, the present study was designed to study anti-asthmatic activity of hydroalcoholic extract of Luffa cylindrica leaves (HAELC) using histamine induced contraction of goat tracheal chain, Clonidine induced catalepsy, Milk induced eosinophilia, Passive paw anaphylaxis and OVA induced airway inflammation. The total phenolic content, total flavonoid content and total alkaloid content was measured by colorimetric assay. In the present study, pretreatment with HAELC (250, 500 and 1000 mg/kg p.o.) significantly inhibited clonidine induced catalepsy, decreased milk induced eosinophilia, inhibited passive paw anaphylaxis and decreased number of eosinophil and macrophage count in the BALF in OVA induced airway inflammation Moreover, histopathological analysis revealed that HAELC treatment suppressed the infiltration of inflammatory cells and the hyperplasia of goblet cells. Thus, the results confirmed anti-asthmatic potential of HAELC. From the results of present investigation, it can be concluded that HAELC possess significant anti-asthmatic activity which may be ascribed to its anti-allergic, bronchodilating, anti-histaminic, adaptogenic, and anti-inflammatory potential, confirming the traditional claim about Luffa cylindrica.

Keywords

Luffa cylindrica anti-asthmatic anti-allergic total phenolic content adaptogenic

References

  • [1] Kelly HW and Sorknes CA. Asthma. In: Dipiro JT, Talbert RL, Yee GC, Matzke TR, Wells BG, Posey LM. (Eds). Pharmacotherapy - A Pathophysiological Approach. The McGraw-Hill, New York, 2005, pp. 504-509.
  • [2] WHO Chronic respiratory diseases: Asthma. http://www.who.int/respiratory/asthma/en/ (accessed on April 20, 2019).
  • [3] Clarke R, Lundy FT, McGarvey L. Herbal treatment in asthma and COPD – current evidence. Clin Phytosci. 2015; 1:4. [CrossRef]
  • [4] Anonymous. Charaka Samhita, Vol 4. Shri Gulabkunverba Ayurvedic Society, Ayurvedic Mundranalaya, Jamangar 1949.
  • [5] Velmurugan V, Shiny G, Surya SP. Phytochemical and Biological Screening of Luffa cylindrica Linn. Fruit. Int J Pharm Tech Res. 2011; 3(3): 1582-1585.
  • [6] Muthumani P, Meera R, Subin M, Jeenamathew, Devi P, Kameswari B, Eswarapriya B. Phytochemical Screening and Anti-inflammatory, Bronchodilator and Antimicrobial activities of the Seeds of Luffa cylindrica. Res J Pharm Biol Chem Sci. 2010; 1(4): 11-22.
  • [7] Zhou X, Wang F, Zhou R, Song X, Xie M. Apigenin: A current review on its beneficial biological activities. J Food Biochem. 2017; 41: e12376. [CrossRef]
  • [8] Shete RV, Pawashe PM, Kore KJ, Otari KV. Protective Role of Luffa cylindrica Linn against Erythromycin Estolate Induced Hepatotoxicity. Current Pharma Research. 2011; 1(4): 315-319. [CrossRef]
  • [9] Harborne JB. Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis. Third ed., Chapman and Hall, London 1998.
  • [10] Marinova D, Ribarova F, Atanassova M. Total phenolics and total flavonoids in bulgarian fruits and vegetables. J Univ Chem Technol Metallurgy. 2005; 40(3): 255-60.
  • [11] Shamsa F, Monsef HR, Ghamooshi R. Spectrophotometric determination of total alkaloids in Peganum harmala L. using bromocresol green. Res J Phytochem. 2007; 1(2): 79-82.
  • [12] OECD, Test No. 423: Acute Oral toxicity - Acute Toxic Class Method, OECD Guidelines for the Testing of Chemicals, Section 4, OECD Publishing, Paris 2002.
  • [13] Nagore DH, Ghosh VK, Patil MJ. Evaluation of antiasthmatic activity of Cassia sophera Linn. Pharmacogn Mag. 2009; 5(19): 109-118.
  • [14] Kumar D, Prasad DN, Parkash J, Bhatnagar SP, Kumar D. Antiasthmatic activity of ethanolic extract of Aerva lanata Linn. Pharmacologyonline. 2009; 2: 1075-1081.
  • [15] Gokhale AB, Saraf MN. Studies on antiallergic activity of ethanolic extract of Tephrosia purpurea Linn. Indian Drugs. 2000; 37(5): 228-232.
  • [16] Lee DS, Park WS, Heo SJ, Cha SH, Kim D, Jeon YJ, Park SG, Seo SK, Choi JS, Park SJ, Shim EB, Choi IW, Jung WK. Polyopes affinis alleviates airway inflammation in a murine model of allergic asthma. J Biosci. 2011; 36(5): 869–877. [CrossRef]
  • [17] National Asthma Education and Prevention Program, Third Expert Panel on the Diagnosis and Management of Asthma. Expert Panel Report 3: Guidelines for the Diagnosis and Management of Asthma. Bethesda (MD): National Heart, Lung, and Blood Institute (US); 2007 Aug. Section 2, Definition, Pathophysiology and Pathogenesis of Asthma, and Natural History of Asthma. Available from: https://www.ncbi.nlm.nih.gov/books/NBK7223/
  • [18] Yamauchi K, Ogasawara M. The Role of Histamine in the Pathophysiology of Asthma and the Clinical Efficacy of Antihistamines in Asthma Therapy. Int J Mol Sci. 2019; 20(7): 1733. [CrossRef]
  • [19] Gosens R, Gross N. The mode of action of anticholinergics in asthma. Eur Respir J. 2018; 52 (4): 1701247. [CrossRef]
  • [20] Jadhav JH, Balsara JJ, Chandorkar AG. Involvement of histaminergic mechanisms in the cataleptogenic effect of clonidine in mice. J Pharm Pharmacol. 1983; 35: 671–673. [CrossRef]
  • [21] Bakakos A, Loukides S, Bakakos P. Severe Eosinophilic Asthma. J Clin Med. 2019; 8(9):1375. [CrossRef]
  • [22] Cockcroft DW. Allergen-induced asthma. Can Respir J. 2014; 21 (5): 279–282. [CrossRef]
  • [23] Aun MV, Bonamichi-Santos R, Arantes-Costa FM, Kalil J, Giavina-Bianchi P. Animal models of asthma: utility and limitations. J Asthma Allergy. 2017; 10: 293–301. [CrossRef]
  • [24] Mathew JE, Srinivasan KK, Dinakaran V, Joseph A. Mast cell stabilizing effects of Sphaeranthus indicus. J Ethnopharmacol. 2009; 122(2): 394–396. [CrossRef]
  • [25] Pungle P, Banavalikar M, Suthar A, Biyani M, Mengi S. Immunomodulatory activity of boswellic acids of Boswellia serrata Roxb. Indian J Exp Biol. 2003; 41(12): 1460-1462.
  • [26] Galli SJ, Grimbaldeston M, Tsai M. Immunomodulatory mast cells: negative, as well as positive, regulators of immunity. Nat Rev Immunol. 2008; 8(6): 478–486. [CrossRef]
  • [27] Busse WW, Rosenwasser LJ. Mechanisms of asthma. J Allergy Clin Immunol. 2003; 111(3): 799–804. [CrossRef]
There are 27 citations in total.

Details

Primary Language English
Subjects Pharmacology and Pharmaceutical Sciences (Other)
Journal Section Articles
Authors

Suvarna Ingale

Prasad Upadhye

Pramod Ingale

Publication Date June 27, 2025
Published in Issue Year 2020 Volume: 24 Issue: 6

Cite

APA Ingale, S., Upadhye, P., & Ingale, P. (2025). Evaluation anti-asthmatic activity of hydroalcoholic extract of Luffa cylindrica leaves. Journal of Research in Pharmacy, 24(6), 855-864. https://doi.org/10.35333/jrp.2020.244
AMA Ingale S, Upadhye P, Ingale P. Evaluation anti-asthmatic activity of hydroalcoholic extract of Luffa cylindrica leaves. J. Res. Pharm. June 2025;24(6):855-864. doi:10.35333/jrp.2020.244
Chicago Ingale, Suvarna, Prasad Upadhye, and Pramod Ingale. “Evaluation Anti-Asthmatic Activity of Hydroalcoholic Extract of Luffa Cylindrica Leaves”. Journal of Research in Pharmacy 24, no. 6 (June 2025): 855-64. https://doi.org/10.35333/jrp.2020.244.
EndNote Ingale S, Upadhye P, Ingale P (June 1, 2025) Evaluation anti-asthmatic activity of hydroalcoholic extract of Luffa cylindrica leaves. Journal of Research in Pharmacy 24 6 855–864.
IEEE S. Ingale, P. Upadhye, and P. Ingale, “Evaluation anti-asthmatic activity of hydroalcoholic extract of Luffa cylindrica leaves”, J. Res. Pharm., vol. 24, no. 6, pp. 855–864, 2025, doi: 10.35333/jrp.2020.244.
ISNAD Ingale, Suvarna et al. “Evaluation Anti-Asthmatic Activity of Hydroalcoholic Extract of Luffa Cylindrica Leaves”. Journal of Research in Pharmacy 24/6 (June 2025), 855-864. https://doi.org/10.35333/jrp.2020.244.
JAMA Ingale S, Upadhye P, Ingale P. Evaluation anti-asthmatic activity of hydroalcoholic extract of Luffa cylindrica leaves. J. Res. Pharm. 2025;24:855–864.
MLA Ingale, Suvarna et al. “Evaluation Anti-Asthmatic Activity of Hydroalcoholic Extract of Luffa Cylindrica Leaves”. Journal of Research in Pharmacy, vol. 24, no. 6, 2025, pp. 855-64, doi:10.35333/jrp.2020.244.
Vancouver Ingale S, Upadhye P, Ingale P. Evaluation anti-asthmatic activity of hydroalcoholic extract of Luffa cylindrica leaves. J. Res. Pharm. 2025;24(6):855-64.