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Tükürük Kinürenin Yolu Metabolitleri Periodontitiste Psikolojik Stresin Bir Biyobelirteci Olabilir mi?

Year 2024, Volume: 3 Issue: 1, 369 - 379, 28.01.2025

Ebru Afşar , Serdar Sütcü , Fatma Bilgesu Erden

https://doi.org/10.58241/ksbd.30

Abstract

Amaç:Bu çalışmada, periodontitisli (PD) bireylerin tükürük örneklerinde kortizol seviyeleri ve kinürenin (KYN) metabolizması değişiklikleri ile periodontal parametreler arasındaki ilişki araştırıldı.
Yöntem: 20 periodontitis hastasından ve 20 periodontal olarak sağlıklı kontrol bireyinden tükürük örnekleri toplandı. Örnekler kortizol, interferon-gama (IFN-γ), Triptofan (TRP), KYN, kinürenik asit (KYNA) ve kinolinik asit (QA) açısından Elisa kiti ile ölçülerek analiz edildi. İndolamin-pirol 2,3-dioksijenaz (IDO) aktivitesi KYN/TRP oranı kullanılarak hesaplandı. Klinik periodontal parametreler olan (plak indeksi (PI), sondalama cebi derinliği (PPD), dişeti çekilmesi (GR), klinik ataşman kaybı (CAL) ve sondalama sırasında kanama (BOP) kaydedildi. Bulgular: Klinik parametreler periodontitis grubunda anlamlı derecede daha yüksekti (p<0,001). Tükürük Kortizol, IFN-γ, TRP, KYN, KYNA, QA düzeyleri ve KYN/TRP oranı periodontitis grubunda kontrol grubuna göre anlamlı derecede daha yüksekti (p<0,001). PPD, BOP, PI ve CAL tükürük kortizol düzeyleriyle anlamlı derecede pozitif korelasyon gösterdi. Ayrıca tükürük kortizol düzeyleri tükürük KYN/TRP oranıyla anlamlı derecede pozitif korelasyon gösterdi.
Sonuç: Bulgularımız stres belirteci kortizolün periodontal inflamasyon ve triptofan-kinürenin metabolizmasında rol oynadığını göstermektedir

Keywords

Kinürenin Kortizol Periodontitis Stres

Ethical Statement

Çalışma, Kapadokya Üniversitesi Girişimsel Olmayan Klinik Etik Kurulu'ndan (14.08.2024 tarihli 24.15 sayılı) onay alındıktan sonra yürütüldü. Tüm katılımcılara çalışma hakkında bilgi verildi ve bilgilendirilmiş onamları sağlandı.

References

  • Aggarwal, K., Gupta, J., Kaur, R. K., Bansal, D., & Jain, A. (2022). Effect of anxiety and psychologic stress on periodontal health: a systematic review and meta-analysis. Quintessence International,53(2), 144-154. https://doi.org/10.3290/j.qi.b2091191
  • Badawy, A. A. (2020). Kynurenine pathway and human systems. Exp Gerontol,129, 110770. https://doi.org/10.1016/j.exger.2019.110770
  • Ball, J., & Darby, I. (2022). Mental health and periodontal and peri-implant diseases. Periodontol 2000,90(1), 106-124. https://doi.org/10.1111/prd.12452
  • Bellavance, M. A., & Rivest, S. (2014). The HPA -Immune Axis and the Immunomodulatory Actions of Glucocorticoids in the Brain. Front Immunol,5, 136. https://doi.org/10.3389/fimmu.2014.00136
  • Botelho, J., Machado, V., Mascarenhas, P., Rua, J., Alves, R., Cavacas, M. A., Delgado, A., & Mendes, J. J. (2018). Stress, salivary cortisol and periodontitis: A systematic review and meta-analysis of observational studies. Archives of Oral Biology,96, 58-65. https://doi.org/10.1016/j.archoralbio.2018.08.016
  • Chen, Y., & Guillemin, G. J. (2009). Kynurenine pathway metabolites in humans: disease and healthy States. Int J Tryptophan Res,2, 1-19. https://doi.org/10.4137/ijtr.s2097
  • Comai, S., Costa, C. V., Ragazzi, E., Bertazzo, A., & Allegri, G. (2005). The effect of age on the enzyme activities of tryptophan metabolism along the kynurenine pathway in rats. Clin Chim Acta,360(1-2), 67-80. https://doi.org/10.1016/j.cccn.2005.04.013
  • Decker, A., Askar, H., Tattan, M., Taichman, R., & Wang, H. L. (2020). The assessment of stress, depression, and inflammation as a collective risk factor for periodontal diseases: a systematic review. Clinical Oral Investigations,24(1), 1-12. https://doi.org/10.1007/s00784-019-03089-3
  • Dornbierer, D. A., Boxler, M., Voegel, C. D., Stucky, B., Steuer, A. E., Binz, T. M., Baumgartner, M. R., Baur, D. M., Quednow, B. B., ...,& Bosch, O. G. (2019). Nocturnal Gamma-Hydroxybutyrate Reduces Cortisol-Awakening Response and Morning Kynurenine Pathway Metabolites in Healthy Volunteers. International Journal of Neuropsychopharmacology,22(10), 631-639. https://doi.org/10.1093/ijnp/pyz047
  • Hunter, R. G., McCarthy, K. J., Milne, T. A., Pfaff, D. W., & McEwen, B. S. (2009). Regulation of hippocampal H3 histone methylation by acute and chronic stress. Proc Natl Acad Sci U S A,106(49), 20912-20917. https://doi.org/10.1073/pnas.0911143106
  • Ishisaka, A., Ansai, T., Soh, I., Inenaga, K., Awano, S., Yoshida, A., Hamasaki, T., Sonoki, K., Takata, Y.,..., & Takehara, T. (2008). Association of cortisol and dehydroepiandrosterone sulphate levels in serum with periodontal status in older Japanese adults. Journal of Clinical Periodontology,35(10), 853-861. https://doi.org/10.1111/j.1600-051X.2008.01309.x
  • Kapoor, V., Kapoor, R., & Chalmers, J. (1994). Kynurenic Acid, an Endogenous Glutamate Antagonist, in Shr and Wky Rats -Possible Role in Central Blood-Pressure Regulation. Clinical and Experimental Pharmacology and Physiology,21(11), 891-896. https://doi.org/DOI10.1111/j.1440-1681.1994.tb02460.x
  • Kelly, J. R., Borre, Y., C, O. B., Patterson, E., El Aidy, S., Deane, J., Kennedy, P. J., Beers, S., Scott, K., Moloney, G., ..., & Dinan, T. G. (2016). Transferring the blues: Depression-associated gut microbiota induces neurobehavioural changes in the rat. J Psychiatr Res,82, 109-118. https://doi.org/10.1016/j.jpsychires.2016.07.019
  • Kinane, D. F., Stathopoulou, P. G., & Papapanou, P. N. (2017). Periodontal diseases. Nat Rev Dis Primers,3, 17038. https://doi.org/10.1038/nrdp.2017.38
  • Koziel, K., & Urbanska, E. M. (2023). Kynurenine Pathway in Diabetes Mellitus-Novel Pharmacological Target? Cells,12(3). https://doi.org/ARTN46010.3390/cells12030460
  • Kubacka, J., Staniszewska, M., Sadok, I., Sypniewska, G., & Stefanska, A. (2022). The Kynurenine Pathway in Obese Middle-Aged Women with Normoglycemia and Type 2 Diabetes. Metabolites,12(6). https://doi.org/10.3390/metabo12060492
  • Kurgan, S., Önder, C., Balci, N., Akdogan, N., Altingöz, S. M., Serdar, M. A., & Günhan, M. (2022). Influence of periodontal inflammation on tryptophan-kynurenine metabolism: a cross-sectional study. Clinical Oral Investigations,26(9), 5721-5732. https://doi.org/10.1007/s00784-022-04528-4
  • Lee, Y. H., Suk, C., Shin, S. I., & Hong, J. Y. (2023). Salivary cortisol, dehydroepiandrosterone, and chromogranin A levels in patients with gingivitis and periodontitis and a novel biomarker for psychological stress. Front Endocrinol (Lausanne),14, 1147739. https://doi.org/10.3389/fendo.2023.1147739
  • Lennartsson, A. K., Theorell, T., Kushnir, M. M., Bergquist, J., & Jonsdottir, I. H. (2013). Perceived stress at work is associated with attenuated DHEA-S response during acute psychosocial stress. Psychoneuroendocrinology,38(9), 1650-1657. https://doi.org/10.1016/j.psyneuen.2013.01.010
  • Liccardo, D., Cannavo, A., Spagnuolo, G., Ferrara, N., Cittadini, A., Rengo, C., & Rengo, G. (2019). Periodontal Disease: A Risk Factor for Diabetes and Cardiovascular Disease. Int J Mol Sci,20(6). https://doi.org/10.3390/ijms20061414
  • Messaoud, A., Rym, M., Wahiba, D., Neffati, F., Najjar, M. F., Gobbi, G., Manchia, M., Valtorta, F., Lotfi, G., & Comai, S. (2022). Investigation of the Relationship among Cortisol, Pro-inflammatory Cytokines, and the Degradation of Tryptophan into Kynurenine in Patients with Major Depression and Suicidal Behavior. Current Topics in Medicinal Chemistry,22(25), 2119-2125. https://doi.org/10.2174/1568026621666210909160210
  • Moon, J. S., Cheong, N. R., Yang, S. Y., Kim, I. S., Chung, H. J., Jeong, Y. W., Park, J. C., Kim, M. S., ..., & Ko, H. M. (2013). Lipopolysaccharide-induced indoleamine 2,3-dioxygenase expression in the periodontal ligament. J Periodontal Res,48(6), 733-739. https://doi.org/10.1111/jre.12063
  • Nikolaus, S., Schulte, B., Al-Massad, N., Thieme, F., Schulte, D. M., Bethge, J., Rehman, A., Tran, F., Aden, K., ..., & Schreiber, S. (2017). Increased Tryptophan Metabolism Is Associated With Activity of Inflammatory Bowel Diseases. Gastroenterology,153(6), 1504-+. https://doi.org/10.1053/j.gastro.2017.08.028
  • Nisapakultorn, K., Makrudthong, J., Sa-Ard-Iam, N., Rerkyen, P., Mahanonda, R., & Takikawa, O. (2009). Indoleamine 2,3-dioxygenase expression and regulation in chronic periodontitis. J Periodontol,80(1), 114-121. https://doi.org/10.1902/jop.2009.080315
  • Obulareddy, V. T., Chava, V. K., & Nagarakanti, S. (2018). Association of Stress, Salivary Cortisol, and Chronic Periodontitis: A Clinico-biochemical Study. Contemp Clin Dent,9(Suppl 2), S299-S304. https://doi.org/10.4103/ccd.ccd_289_18
  • Strasser, B., Berger, K., & Fuchs, D. (2015). Effects of a caloric restriction weight loss diet on tryptophan metabolism and inflammatory biomarkers in overweight adults. Eur J Nutr,54(1), 101-107. https://doi.org/10.1007/s00394-014-0690-3
  • Tonetti, M. S., Greenwell, H., & Kornman, K. S. (2018). Staging and grading of periodontitis: Framework and proposal of a new classification and case definition. J Periodontol,89 Suppl 1, S159-S172. https://doi.org/10.1002/JPER.18-0006
  • Vernerová, A., Krcmová, L. K., Heneberk, O., Radochová, V., & Svec, F. (2022). Liquid chromatography method with tandem mass spectrometry and fluorescence detection for determination of inflammatory biomarkers in gingival crevicular fluid as a tool for diagnosis of periodontal disease. Journal of Pharmaceutical and Biomedical Analysis,212.https://doi.org/10.1016/j.jpba.2022.114644

Tükürük Kinürenin Yolu Metabolitleri Periodontitiste Psikolojik Stresin Bir Biyobelirteci Olabilir mi?

Year 2024, Volume: 3 Issue: 1, 369 - 379, 28.01.2025

Ebru Afşar , Serdar Sütcü , Fatma Bilgesu Erden

https://doi.org/10.58241/ksbd.30

Abstract

Amaç:Bu çalışmada, periodontitisli (PD) bireylerin tükürük örneklerinde kortizol seviyeleri ve kinürenin (KYN) metabolizması değişiklikleri ile periodontal parametreler arasındaki ilişki araştırıldı.
Yöntem: 20 periodontitis hastasından ve 20 periodontal olarak sağlıklı kontrol bireyinden tükürük örnekleri toplandı. Örnekler kortizol, interferon-gama (IFN-γ), Triptofan (TRP), KYN, kinürenik asit (KYNA) ve kinolinik asit (QA) açısından Elisa kiti ile ölçülerek analiz edildi. İndolamin-pirol 2,3-dioksijenaz (IDO) aktivitesi KYN/TRP oranı kullanılarak hesaplandı. Klinik periodontal parametreler olan (plak indeksi (PI), sondalama cebi derinliği (PPD), dişeti çekilmesi (GR), klinik ataşman kaybı (CAL) ve sondalama sırasında kanama (BOP) kaydedildi. Bulgular: Klinik parametreler periodontitis grubunda anlamlı derecede daha yüksekti (p<0,001). Tükürük Kortizol, IFN-γ, TRP, KYN, KYNA, QA düzeyleri ve KYN/TRP oranı periodontitis grubunda kontrol grubuna göre anlamlı derecede daha yüksekti (p<0,001). PPD, BOP, PI ve CAL tükürük kortizol düzeyleriyle anlamlı derecede pozitif korelasyon gösterdi. Ayrıca tükürük kortizol düzeyleri tükürük KYN/TRP oranıyla anlamlı derecede pozitif korelasyon gösterdi.
Sonuç: Bulgularımız stres belirteci kortizolün periodontal inflamasyon ve triptofan-kinürenin metabolizmasında rol oynadığını göstermektedir.

Keywords

Kinürenin Kortizol Periodontitis Stres

Ethical Statement

Çalışma, Kapadokya Üniversitesi Girişimsel Olmayan Klinik Etik Kurulu'ndan (14.08.2024 tarihli 24.15 sayılı) onay alındıktan sonra yürütüldü. Tüm katılımcılara çalışma hakkında bilgi verildi ve bilgilendirilmiş onamları sağlandı.

References

  • Aggarwal, K., Gupta, J., Kaur, R. K., Bansal, D., & Jain, A. (2022). Effect of anxiety and psychologic stress on periodontal health: a systematic review and meta-analysis. Quintessence International,53(2), 144-154. https://doi.org/10.3290/j.qi.b2091191
  • Badawy, A. A. (2020). Kynurenine pathway and human systems. Exp Gerontol,129, 110770. https://doi.org/10.1016/j.exger.2019.110770
  • Ball, J., & Darby, I. (2022). Mental health and periodontal and peri-implant diseases. Periodontol 2000,90(1), 106-124. https://doi.org/10.1111/prd.12452
  • Bellavance, M. A., & Rivest, S. (2014). The HPA -Immune Axis and the Immunomodulatory Actions of Glucocorticoids in the Brain. Front Immunol,5, 136. https://doi.org/10.3389/fimmu.2014.00136
  • Botelho, J., Machado, V., Mascarenhas, P., Rua, J., Alves, R., Cavacas, M. A., Delgado, A., & Mendes, J. J. (2018). Stress, salivary cortisol and periodontitis: A systematic review and meta-analysis of observational studies. Archives of Oral Biology,96, 58-65. https://doi.org/10.1016/j.archoralbio.2018.08.016
  • Chen, Y., & Guillemin, G. J. (2009). Kynurenine pathway metabolites in humans: disease and healthy States. Int J Tryptophan Res,2, 1-19. https://doi.org/10.4137/ijtr.s2097
  • Comai, S., Costa, C. V., Ragazzi, E., Bertazzo, A., & Allegri, G. (2005). The effect of age on the enzyme activities of tryptophan metabolism along the kynurenine pathway in rats. Clin Chim Acta,360(1-2), 67-80. https://doi.org/10.1016/j.cccn.2005.04.013
  • Decker, A., Askar, H., Tattan, M., Taichman, R., & Wang, H. L. (2020). The assessment of stress, depression, and inflammation as a collective risk factor for periodontal diseases: a systematic review. Clinical Oral Investigations,24(1), 1-12. https://doi.org/10.1007/s00784-019-03089-3
  • Dornbierer, D. A., Boxler, M., Voegel, C. D., Stucky, B., Steuer, A. E., Binz, T. M., Baumgartner, M. R., Baur, D. M., Quednow, B. B., ...,& Bosch, O. G. (2019). Nocturnal Gamma-Hydroxybutyrate Reduces Cortisol-Awakening Response and Morning Kynurenine Pathway Metabolites in Healthy Volunteers. International Journal of Neuropsychopharmacology,22(10), 631-639. https://doi.org/10.1093/ijnp/pyz047
  • Hunter, R. G., McCarthy, K. J., Milne, T. A., Pfaff, D. W., & McEwen, B. S. (2009). Regulation of hippocampal H3 histone methylation by acute and chronic stress. Proc Natl Acad Sci U S A,106(49), 20912-20917. https://doi.org/10.1073/pnas.0911143106
  • Ishisaka, A., Ansai, T., Soh, I., Inenaga, K., Awano, S., Yoshida, A., Hamasaki, T., Sonoki, K., Takata, Y.,..., & Takehara, T. (2008). Association of cortisol and dehydroepiandrosterone sulphate levels in serum with periodontal status in older Japanese adults. Journal of Clinical Periodontology,35(10), 853-861. https://doi.org/10.1111/j.1600-051X.2008.01309.x
  • Kapoor, V., Kapoor, R., & Chalmers, J. (1994). Kynurenic Acid, an Endogenous Glutamate Antagonist, in Shr and Wky Rats -Possible Role in Central Blood-Pressure Regulation. Clinical and Experimental Pharmacology and Physiology,21(11), 891-896. https://doi.org/DOI10.1111/j.1440-1681.1994.tb02460.x
  • Kelly, J. R., Borre, Y., C, O. B., Patterson, E., El Aidy, S., Deane, J., Kennedy, P. J., Beers, S., Scott, K., Moloney, G., ..., & Dinan, T. G. (2016). Transferring the blues: Depression-associated gut microbiota induces neurobehavioural changes in the rat. J Psychiatr Res,82, 109-118. https://doi.org/10.1016/j.jpsychires.2016.07.019
  • Kinane, D. F., Stathopoulou, P. G., & Papapanou, P. N. (2017). Periodontal diseases. Nat Rev Dis Primers,3, 17038. https://doi.org/10.1038/nrdp.2017.38
  • Koziel, K., & Urbanska, E. M. (2023). Kynurenine Pathway in Diabetes Mellitus-Novel Pharmacological Target? Cells,12(3). https://doi.org/ARTN46010.3390/cells12030460
  • Kubacka, J., Staniszewska, M., Sadok, I., Sypniewska, G., & Stefanska, A. (2022). The Kynurenine Pathway in Obese Middle-Aged Women with Normoglycemia and Type 2 Diabetes. Metabolites,12(6). https://doi.org/10.3390/metabo12060492
  • Kurgan, S., Önder, C., Balci, N., Akdogan, N., Altingöz, S. M., Serdar, M. A., & Günhan, M. (2022). Influence of periodontal inflammation on tryptophan-kynurenine metabolism: a cross-sectional study. Clinical Oral Investigations,26(9), 5721-5732. https://doi.org/10.1007/s00784-022-04528-4
  • Lee, Y. H., Suk, C., Shin, S. I., & Hong, J. Y. (2023). Salivary cortisol, dehydroepiandrosterone, and chromogranin A levels in patients with gingivitis and periodontitis and a novel biomarker for psychological stress. Front Endocrinol (Lausanne),14, 1147739. https://doi.org/10.3389/fendo.2023.1147739
  • Lennartsson, A. K., Theorell, T., Kushnir, M. M., Bergquist, J., & Jonsdottir, I. H. (2013). Perceived stress at work is associated with attenuated DHEA-S response during acute psychosocial stress. Psychoneuroendocrinology,38(9), 1650-1657. https://doi.org/10.1016/j.psyneuen.2013.01.010
  • Liccardo, D., Cannavo, A., Spagnuolo, G., Ferrara, N., Cittadini, A., Rengo, C., & Rengo, G. (2019). Periodontal Disease: A Risk Factor for Diabetes and Cardiovascular Disease. Int J Mol Sci,20(6). https://doi.org/10.3390/ijms20061414
  • Messaoud, A., Rym, M., Wahiba, D., Neffati, F., Najjar, M. F., Gobbi, G., Manchia, M., Valtorta, F., Lotfi, G., & Comai, S. (2022). Investigation of the Relationship among Cortisol, Pro-inflammatory Cytokines, and the Degradation of Tryptophan into Kynurenine in Patients with Major Depression and Suicidal Behavior. Current Topics in Medicinal Chemistry,22(25), 2119-2125. https://doi.org/10.2174/1568026621666210909160210
  • Moon, J. S., Cheong, N. R., Yang, S. Y., Kim, I. S., Chung, H. J., Jeong, Y. W., Park, J. C., Kim, M. S., ..., & Ko, H. M. (2013). Lipopolysaccharide-induced indoleamine 2,3-dioxygenase expression in the periodontal ligament. J Periodontal Res,48(6), 733-739. https://doi.org/10.1111/jre.12063
  • Nikolaus, S., Schulte, B., Al-Massad, N., Thieme, F., Schulte, D. M., Bethge, J., Rehman, A., Tran, F., Aden, K., ..., & Schreiber, S. (2017). Increased Tryptophan Metabolism Is Associated With Activity of Inflammatory Bowel Diseases. Gastroenterology,153(6), 1504-+. https://doi.org/10.1053/j.gastro.2017.08.028
  • Nisapakultorn, K., Makrudthong, J., Sa-Ard-Iam, N., Rerkyen, P., Mahanonda, R., & Takikawa, O. (2009). Indoleamine 2,3-dioxygenase expression and regulation in chronic periodontitis. J Periodontol,80(1), 114-121. https://doi.org/10.1902/jop.2009.080315
  • Obulareddy, V. T., Chava, V. K., & Nagarakanti, S. (2018). Association of Stress, Salivary Cortisol, and Chronic Periodontitis: A Clinico-biochemical Study. Contemp Clin Dent,9(Suppl 2), S299-S304. https://doi.org/10.4103/ccd.ccd_289_18
  • Strasser, B., Berger, K., & Fuchs, D. (2015). Effects of a caloric restriction weight loss diet on tryptophan metabolism and inflammatory biomarkers in overweight adults. Eur J Nutr,54(1), 101-107. https://doi.org/10.1007/s00394-014-0690-3
  • Tonetti, M. S., Greenwell, H., & Kornman, K. S. (2018). Staging and grading of periodontitis: Framework and proposal of a new classification and case definition. J Periodontol,89 Suppl 1, S159-S172. https://doi.org/10.1002/JPER.18-0006
  • Vernerová, A., Krcmová, L. K., Heneberk, O., Radochová, V., & Svec, F. (2022). Liquid chromatography method with tandem mass spectrometry and fluorescence detection for determination of inflammatory biomarkers in gingival crevicular fluid as a tool for diagnosis of periodontal disease. Journal of Pharmaceutical and Biomedical Analysis,212.https://doi.org/10.1016/j.jpba.2022.114644
There are 28 citations in total.

Details

Primary Language Turkish
Subjects Clinical Sciences (Other)
Journal Section Kapadokya Sağlık Bilimleri Dergisi
Authors

Ebru Afşar 0000-0002-7817-855X

Serdar Sütcü 0000-0002-6255-1428

Fatma Bilgesu Erden 0009-0001-8422-2617

Publication Date January 28, 2025
Submission Date October 30, 2024
Acceptance Date December 2, 2024
Published in Issue Year 2024 Volume: 3 Issue: 1

Cite

APA Afşar, E., Sütcü, S., & Erden, F. B. (2025). Tükürük Kinürenin Yolu Metabolitleri Periodontitiste Psikolojik Stresin Bir Biyobelirteci Olabilir mi?. Kapadokya Sağlık Bilimleri Dergisi, 3(1), 369-379. https://doi.org/10.58241/ksbd.30
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