Objective: This study aimed to evaluate the effects of NADPH oxidase-2 (NOX-2) inhibitor apocynin (APC) on high-fat diet (HFD)-
induced renal and bladder injury.
Materials and Methods: Wistar albino rats were divided into 4 groups: Control, HFD, HFD+dimetyl sulfoxide (DMSO), and HFD+APC.
Rats in HFD, HFD+DMSO, and HFD+APC groups were fed with HFD for sixteen weeks. In the last 4 weeks of the experiment, either
DMSO or APC (25 mg/kg, dissolved in DMSO) was applied to the HFD+DMSO or HFD+APC groups. Lipid profiles and leptin
values were measured in blood serum. Renal and bladder oxidant/antioxidant parameters, histological changes in the tissues, NOX-2-,
nuclear factor kappa B (NF-ĸB)-immunopositive and apoptotic cells were evaluated.
Results: At the end of the experiment, leptin, cholesterol, and triglyceride levels were higher and high-density lipoprotein levels were
lower in the HFD and HFD+DMSO groups compared to controls. In these experimental groups, an increase in malondialdehyde,
8-hydroxy-deoxyguanosine and myeloperoxidase levels and a decrease in glutathione levels, as well as an increase in collagen, NOX-2
– and NFĸ-B-immunopositive and apoptotic cells were found. Also, a deterioration in kidney and bladder morphology was observed.
All these biochemical and histopathological findings improved in the HFD+APC group.
Conclusion: High-fed diet causes renal and bladder injury by increasing NOX-2 activity and inflammation via oxidative stress. APC
might alleviate tissue injury by inhibiting oxidative stress.
Primary Language | English |
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Subjects | Surgery (Other) |
Journal Section | Original Research |
Authors | |
Publication Date | May 30, 2025 |
Submission Date | August 8, 2024 |
Acceptance Date | February 7, 2025 |
Published in Issue | Year 2025 Volume: 38 Issue: 2 |