Research Article
Year 2023,
Volume: 24 Issue: 1, 13 - 18, 30.03.2023
Abstract
References
- 1. Parirokh M, Torabinejad M. Mineral trioxide aggregate: a comprehensive literature review--Part I: chemical, physical, and antibacterial properties. J Endod 2010; 36: 16-27.
- 2. Zhang H, Shen Y, Ruse ND, Haapasalo M. Antibacterial activity of endodontic sealers by modified direct contact test against Enterococcus faecalis. J Endod 2009; 35: 1051-5.
- 3. Shin M, Chen JW, Tsai CY, Aprecio R, Zhang W, Yochim JM, et al. Cytotoxicity and Antimicrobial Effects of a New Fast-Set MTA. Biomed Res Int 2017; 2017: 2071247.
- 4. Torshabi M, Amid R, Kadkhodazadeh M, Shahrbabaki SE, Tabatabaei FS. Cytotoxicity of two available mineral trioxide aggregate cements and a new formulation on human gingival fibroblasts. J Conserv Dent 2016; 19: 522-6.
- 5. Bozkurt AP, Ünlü Ö, Demirci M. Comparison of microbial adhesion and biofilm formation on orthodontic wax materials; an in vitro study. J Dent Sci 2020; 15: 493-9.
- 6. Atasever-Arslan B, Yilancioglu K, Kalkan Z, Timucin AC, Gür H, Isik FB, et al. Screening of new antileukemic agents from essential oils of algae extracts and computational modeling of their interactions with intracellular signaling nodes. Eur J Pharm Sci 2016; 83: 120-31.
- 7. Hilton TJ, Ferracane JL, Mancl L; Northwest Practice-based Research Collaborative in Evidence-based Dentistry (NWP). Comparison of CaOH with MTA for direct pulp capping: a PBRN randomized clinical trial. J Dent Res 2013; 92(7 Suppl): 16S-22S.
- 8. Mente J, Hufnagel S, Leo M, Michel A, Gehrig H, Panagidis D, et al. Treatment outcome of mineral trioxide aggregate or calcium hydroxide direct pulp capping: long-term results. J Endod 2014; 40: 1746-51.
- 9. Luczaj-Cepowicz E, Pawińska M, Marczuk-Kolada G, Leszczyńska K, Waszkiel D. Antibacterial activity of two Mineral Trioxide Aggregate materials in vitro evaluation. Ann Acad Med Stetin 2008; 54: 147-50; discussion 150-1.
- 10. Donyavi Z, Heidari H, Khoshbin K, Shahriari S, Farhadian M, Yousefi Mashouf R. Antibacterial activity of mineral trioxide aggregate, new endodontic cement, Retro MTA and Ortho MTA against common endodontic pathogens. Indo American Journal of Pharmaceutical Sciences 2017; 4: 4720–8.
- 11. Stuart CH, Schwartz SA, Beeson TJ, Owatz CB. Enterococcus faecalis: its role in root canal treatment failure and current concepts in retreatment. J Endod 2006; 32: 93-8.
- 12. Asgary S, Kamrani FA. Antibacterial effects of five different root canal sealing materials. J Oral Sci 2008; 50: 469-74.
- 13. Al-Hezaimi K, Al-Shalan TA, Naghshbandi J, Oglesby S, Simon JH, Rotstein I. Antibacterial effect of two mineral trioxide aggregate (MTA) preparations against Enterococcus faecalis and Streptococcus sanguis in vitro. J Endod 2006; 32: 1053-6.
- 14. Koçak MM, Koçak S, Oktay EA, Kiliç A, Yaman SD. In vitro evaluation of the minimum bactericidal concentrations of different root-end filling materials. J Contemp Dent Pract 2013; 14: 371-4.
- 15. Camilleri J, Montesin FE, Brady K, Sweeney R, Curtis RV, Ford TR. The constitution of mineral trioxide aggregate. Dent Mater 2005; 21: 297-303.
- 16. Sarkar NK, Caicedo R, Ritwik P, Moiseyeva R, Kawashima I. Physicochemical basis of the biologic properties of mineral trioxide aggregate. J Endod 2005; 31: 97-100.
- 17. Yoshimine Y, Ono M, Akamine A. In vitro comparison of the biocompatibility of mineral trioxide aggregate, 4META/MMA-TBB resin, and intermediate restorative material as root-end-filling materials. J Endod 2007; 33: 1066-9.
- 18. Huang FM, Tai KW, Chou MY, Chang YC. Cytotoxicity of resin-, zinc oxide-eugenol-, and calcium hydroxide-based root canal sealers on human periodontal ligament cells and permanent V79 cells. Int Endod J 2002; 35: 153-8.
- 19. Smadi L, Khraisat A, Al-Tarawneh SK, Mahafzah A, Salem A. In vitro evaluation of the antimicrobial activity of nine root canal sealers: direct contact test. Odontostomatol Trop 2008; 31: 11-8.
- 20. Lü L, Zhang L, Wai MS, Yew DT, Xu J. Exocytosis of MTT formazan could exacerbate cell injury. Toxicol In Vitro 2012; 26: 636-44.
- 21. van Tonder A, Joubert AM, Cromarty AD. Limitations of the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay when compared to three commonly used cell enumeration assays. BMC Res Notes 2015;8:47.
- 22. Bowey-Dellinger K, Dixon L, Ackerman K, Vigueira C, Suh YK, Lyda T, et al. Introducing Mammalian Cell Culture and Cell Viability Techniques in the Undergraduate Biology Laboratory. J Microbiol Biol Educ 2017; 18: 18.2.38.
- 23. Cartwright T and Shah P. Culture media In: Basic cell culture: Ed. Davis JM, New York: Oxford Univercity Press, 1998: 57-91.
- 24. Kouchak Dezfouli N, Asnaashari E, Khalilak Z. Comparison of the Biocompatibility of Pro Root MTA, Retro MTA and MTA Plus Using an MTT Assay Study. EC Dental Science 2017; 11: 83-7.
Evaluation of Cytotoxicity and Antibacterial Effect of Different Types of Mineral Trioxide Aggregate
Abstract
Objective: Mineral trioxide aggregate (MTA) has been used as a filling material in endodontic procedures over decades. The newer formulations of MTA were launched in the dental market and their cytotoxic, proliferative and antimicrobial effects need to be revealed. This study compared the possible cytotoxic and proliferative effects on fibroblasts and the antimicrobial activity against Streptococcus mutans, Lactobacillus acidophilus, and Enterococcus faecalis of four different MTAs in the dental market.
Materials and Methods: Cytotoxicity assay was performed on 3T3 fibroblast cell lines were determined using yellow tetrazolium 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, while the antimicrobial activity was tested with the broth microdilution method.
Results: RetroMTA, AngelusMTA, and NeoMTA demonstrated a proliferative effect on 3T3 cells, suggesting induction in tissue repair. Moreover, NeoMTA showed the highest antimicrobial activity against all strains tested.
Conclusion: According to our study, NeoMTA and RetroMTA may be recommended for clinical applications in comparison with the conventional AngelusMTA.
References
- 1. Parirokh M, Torabinejad M. Mineral trioxide aggregate: a comprehensive literature review--Part I: chemical, physical, and antibacterial properties. J Endod 2010; 36: 16-27.
- 2. Zhang H, Shen Y, Ruse ND, Haapasalo M. Antibacterial activity of endodontic sealers by modified direct contact test against Enterococcus faecalis. J Endod 2009; 35: 1051-5.
- 3. Shin M, Chen JW, Tsai CY, Aprecio R, Zhang W, Yochim JM, et al. Cytotoxicity and Antimicrobial Effects of a New Fast-Set MTA. Biomed Res Int 2017; 2017: 2071247.
- 4. Torshabi M, Amid R, Kadkhodazadeh M, Shahrbabaki SE, Tabatabaei FS. Cytotoxicity of two available mineral trioxide aggregate cements and a new formulation on human gingival fibroblasts. J Conserv Dent 2016; 19: 522-6.
- 5. Bozkurt AP, Ünlü Ö, Demirci M. Comparison of microbial adhesion and biofilm formation on orthodontic wax materials; an in vitro study. J Dent Sci 2020; 15: 493-9.
- 6. Atasever-Arslan B, Yilancioglu K, Kalkan Z, Timucin AC, Gür H, Isik FB, et al. Screening of new antileukemic agents from essential oils of algae extracts and computational modeling of their interactions with intracellular signaling nodes. Eur J Pharm Sci 2016; 83: 120-31.
- 7. Hilton TJ, Ferracane JL, Mancl L; Northwest Practice-based Research Collaborative in Evidence-based Dentistry (NWP). Comparison of CaOH with MTA for direct pulp capping: a PBRN randomized clinical trial. J Dent Res 2013; 92(7 Suppl): 16S-22S.
- 8. Mente J, Hufnagel S, Leo M, Michel A, Gehrig H, Panagidis D, et al. Treatment outcome of mineral trioxide aggregate or calcium hydroxide direct pulp capping: long-term results. J Endod 2014; 40: 1746-51.
- 9. Luczaj-Cepowicz E, Pawińska M, Marczuk-Kolada G, Leszczyńska K, Waszkiel D. Antibacterial activity of two Mineral Trioxide Aggregate materials in vitro evaluation. Ann Acad Med Stetin 2008; 54: 147-50; discussion 150-1.
- 10. Donyavi Z, Heidari H, Khoshbin K, Shahriari S, Farhadian M, Yousefi Mashouf R. Antibacterial activity of mineral trioxide aggregate, new endodontic cement, Retro MTA and Ortho MTA against common endodontic pathogens. Indo American Journal of Pharmaceutical Sciences 2017; 4: 4720–8.
- 11. Stuart CH, Schwartz SA, Beeson TJ, Owatz CB. Enterococcus faecalis: its role in root canal treatment failure and current concepts in retreatment. J Endod 2006; 32: 93-8.
- 12. Asgary S, Kamrani FA. Antibacterial effects of five different root canal sealing materials. J Oral Sci 2008; 50: 469-74.
- 13. Al-Hezaimi K, Al-Shalan TA, Naghshbandi J, Oglesby S, Simon JH, Rotstein I. Antibacterial effect of two mineral trioxide aggregate (MTA) preparations against Enterococcus faecalis and Streptococcus sanguis in vitro. J Endod 2006; 32: 1053-6.
- 14. Koçak MM, Koçak S, Oktay EA, Kiliç A, Yaman SD. In vitro evaluation of the minimum bactericidal concentrations of different root-end filling materials. J Contemp Dent Pract 2013; 14: 371-4.
- 15. Camilleri J, Montesin FE, Brady K, Sweeney R, Curtis RV, Ford TR. The constitution of mineral trioxide aggregate. Dent Mater 2005; 21: 297-303.
- 16. Sarkar NK, Caicedo R, Ritwik P, Moiseyeva R, Kawashima I. Physicochemical basis of the biologic properties of mineral trioxide aggregate. J Endod 2005; 31: 97-100.
- 17. Yoshimine Y, Ono M, Akamine A. In vitro comparison of the biocompatibility of mineral trioxide aggregate, 4META/MMA-TBB resin, and intermediate restorative material as root-end-filling materials. J Endod 2007; 33: 1066-9.
- 18. Huang FM, Tai KW, Chou MY, Chang YC. Cytotoxicity of resin-, zinc oxide-eugenol-, and calcium hydroxide-based root canal sealers on human periodontal ligament cells and permanent V79 cells. Int Endod J 2002; 35: 153-8.
- 19. Smadi L, Khraisat A, Al-Tarawneh SK, Mahafzah A, Salem A. In vitro evaluation of the antimicrobial activity of nine root canal sealers: direct contact test. Odontostomatol Trop 2008; 31: 11-8.
- 20. Lü L, Zhang L, Wai MS, Yew DT, Xu J. Exocytosis of MTT formazan could exacerbate cell injury. Toxicol In Vitro 2012; 26: 636-44.
- 21. van Tonder A, Joubert AM, Cromarty AD. Limitations of the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay when compared to three commonly used cell enumeration assays. BMC Res Notes 2015;8:47.
- 22. Bowey-Dellinger K, Dixon L, Ackerman K, Vigueira C, Suh YK, Lyda T, et al. Introducing Mammalian Cell Culture and Cell Viability Techniques in the Undergraduate Biology Laboratory. J Microbiol Biol Educ 2017; 18: 18.2.38.
- 23. Cartwright T and Shah P. Culture media In: Basic cell culture: Ed. Davis JM, New York: Oxford Univercity Press, 1998: 57-91.
- 24. Kouchak Dezfouli N, Asnaashari E, Khalilak Z. Comparison of the Biocompatibility of Pro Root MTA, Retro MTA and MTA Plus Using an MTT Assay Study. EC Dental Science 2017; 11: 83-7.
There are 24 citations in total.
Details
| Primary Language | English |
|---|---|
| Subjects | Clinical Microbiology |
| Journal Section | Research Article |
| Authors | |
| Publication Date | March 30, 2023 |
| Published in Issue | Year 2023 Volume: 24 Issue: 1 |